(Circular) (Six-base) MAPSORT of: pFN21ASDA0582.seq Check: 2148 from: 1 to: 7000 pFN21ASDA0582 7000 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4810 278 Size: 53 83 186 936 3274 2468 Fragments arranged by size: 3274 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3024 4343 1937 Size: 1087 1319 4594 Fragments arranged by size: 4594 1319 1087 Acc65I G'GTAC_C Cuts at: 4321 4321 Size: 7000 AclI AA'CG_TT Cuts at: 1563 5127 5500 1563 Size: 3564 373 3063 Fragments arranged by size: 3564 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2082 4085 5063 6447 6715 1632 Size: 450 2003 978 1384 268 1917 Fragments arranged by size: 2003 1917 1384 978 450 268 AflII C'TTAA_G Cuts at: 820 1017 3146 820 Size: 197 2129 4674 Fragments arranged by size: 4674 2129 197 AflIII A'CryG_T Cuts at: 1203 5899 6876 1203 Size: 4696 977 1327 Fragments arranged by size: 4696 1327 977 AgeI A'CCGG_T Cuts at: 3816 5831 3816 Size: 2015 4985 AhdI GACnn_n'nnGTC Cuts at: 5729 5729 Size: 7000 AleI CACnn'nnGTG Cuts at: 1166 4281 1166 Size: 3115 3885 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7000 AlwNI CAG_nnn'CTG Cuts at: 2425 2512 4063 4108 4743 6315 2425 Size: 87 1551 45 635 1572 3110 Fragments arranged by size: 3110 1572 1551 635 87 45 ApaI G_GGCC'C Cuts at: 2130 3132 2130 Size: 1002 5998 ApaLI G'TGCA_C Cuts at: 5057 6213 5057 Size: 1156 5844 ApoI r'AATT_y Cuts at: 1493 4309 4535 4687 6796 1493 Size: 2816 226 152 2109 1697 Fragments arranged by size: 2816 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5554 160 Size: 872 4522 1606 Fragments arranged by size: 4522 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7000 AvaI C'yCGr_G Cuts at: 1870 1943 2954 3761 4325 1870 Size: 73 1011 807 564 4545 Fragments arranged by size: 4545 1011 807 564 73 BamHI G'GATC_C Cuts at: 3521 3670 4330 4697 3521 Size: 149 660 367 5824 Fragments arranged by size: 5824 660 367 149 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3405 4321 5776 618 Size: 325 217 627 1618 916 1455 1842 Fragments arranged by size: 1842 1618 1455 916 627 325 217 BanII G_rGCy'C Cuts at: 1391 2130 2175 2257 2292 2582 3132 3232 3326 Size: 739 45 82 35 290 550 100 94 Cuts at: 3326 3575 4319 1391 Size: 249 744 4072 Fragments arranged by size: 4072 744 739 550 290 249 100 94 82 45 35 BbeI G_GCGC'C Cuts at: 3409 3409 Size: 7000 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2070 2393 3911 4002 928 Size: 971 171 323 1518 91 3926 Fragments arranged by size: 3926 1518 971 323 171 91 BbvCI CC'TCA_GC Cuts at: 2163 2519 2961 2163 Size: 356 442 6202 Fragments arranged by size: 6202 442 356 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3452 3486 5191 5225 1903 Size: 34 1515 34 1705 34 3678 Fragments arranged by size: 3678 1705 1515 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2196 4892 6102 2196 Size: 2696 1210 3094 Fragments arranged by size: 3094 2696 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7000 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5611 136 Size: 107 122 71 631 605 3939 1525 Fragments arranged by size: 3939 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2923 6995 2923 Size: 4072 2928 BlpI GC'TnA_GC Cuts at: 2576 2595 4417 2576 Size: 19 1822 5159 Fragments arranged by size: 5159 1822 19 Bme1580I G_kGCm'C Cuts at: 1790 2130 3132 3403 3718 5061 6217 1790 Size: 340 1002 271 315 1343 1156 2573 Fragments arranged by size: 2573 1343 1156 1002 340 315 271 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7000 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2071 2755 2851 Size: 416 53 83 399 24 654 684 96 Cuts at: 2851 3561 4118 4731 5689 442 Size: 710 557 613 958 1753 Fragments arranged by size: 1753 958 710 684 654 613 557 416 399 96 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7000 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3671 3671 Size: 7000 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2370 2627 2726 3033 3145 3607 3666 4193 Size: 1131 257 99 307 112 462 59 527 Cuts at: 4193 5660 1239 Size: 1467 2579 Fragments arranged by size: 2579 1467 1131 527 462 307 257 112 99 59 Bpu10I CC'TnA_GC Cuts at: 2163 2455 2519 2539 2961 3335 2163 Size: 292 64 20 422 374 5828 Fragments arranged by size: 5828 422 374 292 64 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3989 5125 5990 6288 3989 Size: 1136 865 298 4701 Fragments arranged by size: 4701 1136 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2346 2549 2698 3772 5663 882 Size: 617 847 203 149 1074 1891 2219 Fragments arranged by size: 2219 1891 1074 847 617 203 149 BsaAI yAC'GTr Cuts at: 493 1204 4297 4791 493 Size: 711 3093 494 2702 Fragments arranged by size: 3093 2702 711 494 BsaBI GATnn'nnATC Cuts at: 4053 6994 4053 Size: 2941 4059 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2818 3406 4807 5189 Size: 53 83 186 936 1285 588 1401 382 Cuts at: 5189 5824 275 Size: 635 1451 Fragments arranged by size: 1451 1401 1285 936 635 588 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3816 5433 5831 6105 6252 6663 3816 Size: 1617 398 274 147 411 4153 Fragments arranged by size: 4153 1617 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2873 2903 1178 Size: 30 165 30 1470 30 5275 Fragments arranged by size: 5275 1470 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2423 2686 2689 3760 3882 1186 Size: 1237 263 3 1071 122 4304 Fragments arranged by size: 4304 1237 1071 263 122 3 BseYI C'CCAG_C Cuts at: 3330 6203 6699 3330 Size: 2873 496 3631 Fragments arranged by size: 3631 2873 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2298 3400 4219 1163 Size: 573 562 1102 819 3944 Fragments arranged by size: 3944 1102 819 573 562 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2715 2884 4450 5211 6239 1540 Size: 275 186 714 169 1566 761 1028 2301 Fragments arranged by size: 2301 1566 1028 761 714 275 186 169 BsiHKAI G_wGCw'C Cuts at: 4278 5061 5146 6217 4278 Size: 783 85 1071 5061 Fragments arranged by size: 5061 1071 783 85 BsmI GAATG_Cn' Cuts at: 4511 4604 4511 Size: 93 6907 BsmBI CGTCTCn'nnnn_ Cuts at: 2809 2809 Size: 7000 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2130 2175 2257 2292 2582 3132 3232 Size: 399 340 45 82 35 290 550 100 Cuts at: 3232 3326 3403 3575 3718 4278 4319 5061 5146 Size: 94 77 172 143 560 41 742 85 Cuts at: 5146 6217 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 560 550 399 340 290 172 143 100 94 85 82 77 45 41 35 BspEI T'CCGG_A Cuts at: 6663 6663 Size: 7000 BspHI T'CATG_A Cuts at: 4889 6619 4889 Size: 1730 5270 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2215 3422 4355 844 Size: 1371 1207 933 3489 Fragments arranged by size: 3489 1371 1207 933 BsrBI CCG'CTC Cuts at: 1394 1823 2107 3482 4887 1394 Size: 429 284 1375 1405 3507 Fragments arranged by size: 3507 1405 1375 429 284 BsrDI GCAATG_nn' Cuts at: 65 1248 3902 4275 5495 5669 65 Size: 1183 2654 373 1220 174 1396 Fragments arranged by size: 2654 1396 1220 1183 373 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2710 3291 3816 4026 5644 5831 1700 Size: 110 900 581 525 210 1618 187 2869 Fragments arranged by size: 2869 1618 900 581 525 210 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7000 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7000 BssSI C'ACGA_G Cuts at: 2670 5060 6072 2670 Size: 2390 1012 3598 Fragments arranged by size: 3598 2390 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7000 BstBI TT'CG_AA Cuts at: 2007 5804 2007 Size: 3797 3203 BstEII G'GTnAC_C Cuts at: 1184 2648 2766 3538 1184 Size: 1464 118 772 4646 Fragments arranged by size: 4646 1464 772 118 BstXI CCAn_nnnn'nTGG Cuts at: 2029 2084 3665 2029 Size: 55 1581 5364 Fragments arranged by size: 5364 1581 55 BstYI r'GATC_y Cuts at: 1972 2923 3521 3662 3670 4330 4697 5082 5099 Size: 951 598 141 8 660 367 385 17 Cuts at: 5099 6551 6637 6649 6995 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 951 660 598 385 367 346 141 86 17 12 8 Bsu36I CC'TnA_GG Cuts at: 3275 3675 3275 Size: 400 6600 BtgI C'CryG_G Cuts at: 513 1065 2022 2340 3970 4260 513 Size: 552 957 318 1630 290 3253 Fragments arranged by size: 3253 1630 957 552 318 290 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6261 Fragments arranged by size: 6261 683 56 BtsI GCAGTG_nn' Cuts at: 3108 3158 4516 5310 5330 3108 Size: 50 1358 794 20 4778 Fragments arranged by size: 4778 1358 794 50 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7000 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6965 DraI TTT'AAA Cuts at: 4304 4304 Size: 7000 DraIII CAC_nnn'GTG Cuts at: 2321 2321 Size: 7000 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6007 809 Size: 507 4691 1802 Fragments arranged by size: 4691 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2685 3821 4447 Size: 54 1425 50 275 18 855 1136 626 Cuts at: 4447 5336 6965 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1136 889 855 626 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4447 1537 Size: 275 2635 4090 Fragments arranged by size: 4090 2635 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3377 3435 3921 4930 1353 Size: 2024 58 486 1009 3423 Fragments arranged by size: 3423 2024 1009 486 58 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2678 5587 5961 6107 387 Size: 1064 138 1089 2909 374 146 1280 Fragments arranged by size: 2909 1280 1089 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2082 2370 2627 2726 3033 3145 3607 Size: 393 450 288 257 99 307 112 462 Cuts at: 3607 3666 4085 4193 5063 5660 6447 6715 1239 Size: 59 419 108 870 597 787 268 1524 Fragments arranged by size: 1524 870 787 597 462 450 419 393 307 288 268 257 112 108 99 59 EcoNI CCTnn'n_nnAGG Cuts at: 3364 3364 Size: 7000 EcoO109I rG'GnC_Cy Cuts at: 2126 2750 3757 2126 Size: 624 1007 5369 Fragments arranged by size: 5369 1007 624 EcoRI G'AATT_C Cuts at: 4309 4687 4309 Size: 378 6622 EcoRV GAT'ATC Cuts at: 3559 3559 Size: 7000 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2945 3869 2945 Size: 924 6076 FspI TGC'GCA Cuts at: 5506 5506 Size: 7000 HaeII r_GCGC'y Cuts at: 1678 3409 6147 1678 Size: 1731 2738 2531 Fragments arranged by size: 2738 2531 1731 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2160 2192 2471 2503 2873 Size: 32 135 32 588 32 279 32 370 Cuts at: 2873 2905 3023 3055 3308 3340 5641 5673 5715 Size: 32 118 32 253 32 2301 32 42 Cuts at: 5715 5747 1373 Size: 32 2626 Fragments arranged by size: 2626 2301 588 370 279 253 135 118 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4344 4590 669 Size: 988 281 2406 246 3079 Fragments arranged by size: 3079 2406 988 281 246 HindIII A'AGCT_T Cuts at: 748 2393 748 Size: 1645 5355 HpaI GTT'AAC Cuts at: 4590 4590 Size: 7000 KasI G'GCGC_C Cuts at: 3405 3405 Size: 7000 KpnI G_GTAC'C Cuts at: 4325 4325 Size: 7000 MfeI C'AATT_G Cuts at: 4599 4599 Size: 7000 MluI A'CGCG_T Cuts at: 6876 6876 Size: 7000 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3792 4092 6114 6298 1305 Size: 2487 300 2022 184 2007 Fragments arranged by size: 2487 2022 2007 300 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2687 3823 10 Size: 54 1425 343 855 1136 3187 Fragments arranged by size: 3187 1425 1136 855 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4281 4958 5317 5476 518 Size: 648 3115 677 359 159 2042 Fragments arranged by size: 3115 2042 677 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2607 2996 3922 4416 5093 6241 6486 1606 Size: 1001 389 926 494 677 1148 245 2120 Fragments arranged by size: 2120 1148 1001 926 677 494 389 245 NarI GG'CG_CC Cuts at: 3406 3406 Size: 7000 NcoI C'CATG_G Cuts at: 513 1065 2022 2340 4260 513 Size: 552 957 318 1920 3253 Fragments arranged by size: 3253 1920 957 552 318 NdeI CA'TA_TG Cuts at: 387 387 Size: 7000 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7000 NotI GC'GGCC_GC Cuts at: 4447 4447 Size: 7000 NspI r_CATG'y Cuts at: 1132 1229 4225 4358 5903 1132 Size: 97 2996 133 1545 2229 Fragments arranged by size: 2996 2229 1545 133 97 PasI CC'CwG_GG Cuts at: 1348 2029 2123 1348 Size: 681 94 6225 Fragments arranged by size: 6225 681 94 PciI A'CATG_T Cuts at: 5899 5899 Size: 7000 PflMI CCAn_nnn'nTGG Cuts at: 1108 2512 3125 1108 Size: 1404 613 4983 Fragments arranged by size: 4983 1404 613 PmeI GTTT'AAAC Cuts at: 4304 4304 Size: 7000 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2569 2601 5069 5101 6582 6614 2569 Size: 32 2468 32 1481 32 2955 Fragments arranged by size: 2955 2468 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2750 2750 Size: 7000 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7000 PsiI TTA'TAA Cuts at: 4570 4570 Size: 7000 PspOMI G'GGCC_C Cuts at: 2126 3128 2126 Size: 1002 5998 PstI C_TGCA'G Cuts at: 830 2102 2612 3012 3982 4020 4352 830 Size: 1272 510 400 970 38 332 3478 Fragments arranged by size: 3478 1272 970 510 400 332 38 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7000 PvuII CAG'CTG Cuts at: 2607 2996 3922 2607 Size: 389 926 5685 Fragments arranged by size: 5685 926 389 SalI G'TCGA_C Cuts at: 1936 4342 1936 Size: 2406 4594 SapI GCTCTTCn'nnn_ Cuts at: 3921 3921 Size: 7000 SbfI CC_TGCA'GG Cuts at: 3012 4352 3012 Size: 1340 5660 ScaI AGT'ACT Cuts at: 2840 5248 6960 2840 Size: 2408 1712 2880 Fragments arranged by size: 2880 2408 1712 SfcI C'TryA_G Cuts at: 826 1045 2098 2608 3008 3300 3978 4016 4348 Size: 219 1053 510 400 292 678 38 332 Cuts at: 4348 5483 6164 6355 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1053 681 678 510 400 332 292 219 191 38 SfoI GGC'GCC Cuts at: 3407 3407 Size: 7000 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7000 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7000 SmaI CCC'GGG Cuts at: 1872 2956 4327 1872 Size: 1084 1371 4545 Fragments arranged by size: 4545 1371 1084 SmlI C'TyrA_G Cuts at: 820 1017 1943 3146 4004 5104 6005 6267 820 Size: 197 926 1203 858 1100 901 262 1553 Fragments arranged by size: 1553 1203 1100 926 901 858 262 197 SnaBI TAC'GTA Cuts at: 493 4297 4791 493 Size: 3804 494 2702 Fragments arranged by size: 3804 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7000 SphI G_CATG'C Cuts at: 1132 4358 1132 Size: 3226 3774 SrfI GCCC'GGGC Cuts at: 2956 2956 Size: 7000 SspI AAT'ATT Cuts at: 5 52 4924 5 Size: 47 4872 2081 Fragments arranged by size: 4872 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7000 StyI C'CwwG_G Cuts at: 513 1065 2022 2340 2688 3534 3576 3658 4141 Size: 552 957 318 348 846 42 82 483 Cuts at: 4141 4260 4439 513 Size: 119 179 3074 Fragments arranged by size: 3074 957 846 552 483 348 318 179 119 82 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2021 4293 5027 5044 5197 5382 5827 Size: 1255 441 2272 734 17 153 185 445 Cuts at: 5827 325 Size: 1498 Fragments arranged by size: 2272 1498 1255 734 445 441 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2838 5246 6958 Size: 274 80 33 51 1443 861 2408 1712 Cuts at: 6958 96 Size: 138 Fragments arranged by size: 2408 1712 1443 861 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3338 3370 6960 6992 3338 Size: 32 3590 32 3346 Fragments arranged by size: 3590 3346 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3002 1316 Size: 1686 5314 XbaI T'CTAG_A Cuts at: 2249 4336 2249 Size: 2087 4913 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3179 3532 3641 4267 3179 Size: 353 109 626 5912 Fragments arranged by size: 5912 626 353 109 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7000 XmaI C'CCGG_G Cuts at: 1870 2954 4325 1870 Size: 1084 1371 4545 Fragments arranged by size: 4545 1371 1084 XmnI GAAnn'nnTTC Cuts at: 5129 5129 Size: 7000 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4808 276 Size: 53 83 186 936 3274 2468 Fragments arranged by size: 3274 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SwaI