(Circular) (Six-base) MAPSORT of: pFN21ASDA0576.seq Check: 5804 from: 1 to: 7912 pFN21ASDA0576 7912 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4409 4409 Size: 7912 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5722 278 Size: 53 83 186 936 4186 2468 Fragments arranged by size: 4186 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5255 1937 Size: 3318 4594 Acc65I G'GTAC_C Cuts at: 5233 5233 Size: 7912 AclI AA'CG_TT Cuts at: 1563 6039 6412 1563 Size: 4476 373 3063 Fragments arranged by size: 4476 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2632 5975 7359 7627 1632 Size: 1000 3343 1384 268 1917 Fragments arranged by size: 3343 1917 1384 1000 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7715 AflIII A'CryG_T Cuts at: 1203 2505 3667 6811 7788 1203 Size: 1302 1162 3144 977 1327 Fragments arranged by size: 3144 1327 1302 1162 977 AgeI A'CCGG_T Cuts at: 6743 6743 Size: 7912 AhdI GACnn_n'nnGTC Cuts at: 2035 3182 6641 2035 Size: 1147 3459 3306 Fragments arranged by size: 3459 3306 1147 AleI CACnn'nnGTG Cuts at: 1166 2510 3340 1166 Size: 1344 830 5738 Fragments arranged by size: 5738 1344 830 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7912 AlwNI CAG_nnn'CTG Cuts at: 5655 7227 5655 Size: 1572 6340 ApaLI G'TGCA_C Cuts at: 4346 4431 5969 7125 4346 Size: 85 1538 1156 5133 Fragments arranged by size: 5133 1538 1156 85 ApoI r'AATT_y Cuts at: 1493 2378 2681 3092 3169 3290 3370 3677 4312 Size: 885 303 411 77 121 80 307 635 Cuts at: 4312 4938 5144 5221 5447 5599 7708 1493 Size: 626 206 77 226 152 2109 1697 Fragments arranged by size: 2109 1697 885 635 626 411 307 303 226 206 152 121 80 77 77 AseI AT'TA_AT Cuts at: 160 1032 2466 6466 160 Size: 872 1434 4000 1606 Fragments arranged by size: 4000 1606 1434 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7912 AvaI C'yCGr_G Cuts at: 1870 1943 2032 5237 1870 Size: 73 89 3205 4545 Fragments arranged by size: 4545 3205 89 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3919 3952 3919 Size: 33 7879 BamHI G'GATC_C Cuts at: 3250 5242 5609 3250 Size: 1992 367 5553 Fragments arranged by size: 5553 1992 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2571 4397 5117 5233 6688 Size: 325 217 627 784 1826 720 116 1455 Cuts at: 6688 618 Size: 1842 Fragments arranged by size: 1842 1826 1455 784 720 627 325 217 116 BanII G_rGCy'C Cuts at: 1391 3012 5231 1391 Size: 1621 2219 4072 Fragments arranged by size: 4072 2219 1621 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2112 5006 928 Size: 971 213 2894 3834 Fragments arranged by size: 3834 2894 971 213 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6103 6137 1903 Size: 34 4166 34 3678 Fragments arranged by size: 4166 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5804 7014 5804 Size: 1210 6702 BclI T'GATC_A Cuts at: 1548 3802 1548 Size: 2254 5658 BfrBI ATG'CAT Cuts at: 4903 4903 Size: 7912 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6523 136 Size: 107 122 71 631 605 4851 1525 Fragments arranged by size: 4851 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7907 7907 Size: 7912 BlpI GC'TnA_GC Cuts at: 5329 5329 Size: 7912 Bme1580I G_kGCm'C Cuts at: 1790 2574 3595 4350 4435 5973 7129 1790 Size: 784 1021 755 85 1538 1156 2573 Fragments arranged by size: 2573 1538 1156 1021 784 755 85 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7912 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2882 4470 5643 Size: 416 53 83 399 24 1465 1588 1173 Cuts at: 5643 6601 442 Size: 958 1753 Fragments arranged by size: 1753 1588 1465 1173 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7912 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5031 5031 Size: 7912 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2046 3359 4997 6572 1239 Size: 807 1313 1638 1575 2579 Fragments arranged by size: 2579 1638 1575 1313 807 Bpu10I CC'TnA_GC Cuts at: 4613 4613 Size: 7912 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5216 6037 6902 7200 5216 Size: 821 865 298 5928 Fragments arranged by size: 5928 865 821 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2022 4160 6575 882 Size: 617 523 2138 2415 2219 Fragments arranged by size: 2415 2219 2138 617 523 BsaAI yAC'GTr Cuts at: 493 1204 2508 5209 5703 493 Size: 711 1304 2701 494 2702 Fragments arranged by size: 2702 2701 1304 711 494 BsaBI GATnn'nnATC Cuts at: 3123 7906 3123 Size: 4783 3129 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5719 6101 6736 275 Size: 53 83 186 936 4186 382 635 1451 Fragments arranged by size: 4186 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6345 6743 7017 7164 7575 6345 Size: 398 274 147 411 6682 Fragments arranged by size: 6682 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4364 4394 1178 Size: 30 165 30 2961 30 4696 Fragments arranged by size: 4696 2961 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2511 2834 4097 4382 4385 1186 Size: 1325 323 1263 285 3 4713 Fragments arranged by size: 4713 1325 1263 323 285 3 BseYI C'CCAG_C Cuts at: 7115 7611 7115 Size: 496 7416 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4387 1163 Size: 573 2651 4688 Fragments arranged by size: 4688 2651 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5362 6123 7151 1540 Size: 275 186 3361 761 1028 2301 Fragments arranged by size: 3361 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3012 3940 4089 4350 4435 5973 6058 7129 3012 Size: 928 149 261 85 1538 85 1071 3795 Fragments arranged by size: 3795 1538 1071 928 261 149 85 85 BsmI GAATG_Cn' Cuts at: 2327 3452 5423 5516 2327 Size: 1125 1971 93 4723 Fragments arranged by size: 4723 1971 1125 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2574 3012 3595 3940 4089 4350 4435 Size: 399 784 438 583 345 149 261 85 Cuts at: 4435 5231 5973 6058 7129 1391 Size: 796 742 85 1071 2174 Fragments arranged by size: 2174 1071 796 784 742 583 438 399 345 261 149 85 85 BspEI T'CCGG_A Cuts at: 7575 7575 Size: 7912 BspHI T'CATG_A Cuts at: 4417 5801 7531 4417 Size: 1384 1730 4798 Fragments arranged by size: 4798 1730 1384 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4409 4674 5267 844 Size: 3565 265 593 3489 Fragments arranged by size: 3565 3489 593 265 BsrBI CCG'CTC Cuts at: 1394 1823 4801 5799 1394 Size: 429 2978 998 3507 Fragments arranged by size: 3507 2978 998 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3345 3848 3982 4456 6407 6581 65 Size: 1183 2097 503 134 474 1951 174 1396 Fragments arranged by size: 2097 1951 1396 1183 503 474 174 134 BsrFI r'CCGG_y Cuts at: 1700 1810 6556 6743 1700 Size: 110 4746 187 2869 Fragments arranged by size: 4746 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4512 96 Size: 4416 3496 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7912 BssSI C'ACGA_G Cuts at: 5972 6984 5972 Size: 1012 6900 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7912 BstBI TT'CG_AA Cuts at: 2007 6716 2007 Size: 4709 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7912 BstYI r'GATC_y Cuts at: 1972 3250 4493 5242 5609 5994 6011 7463 7549 Size: 1278 1243 749 367 385 17 1452 86 Cuts at: 7549 7561 7907 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1278 1243 749 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2073 2073 Size: 7912 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 6403 Fragments arranged by size: 6403 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7173 Fragments arranged by size: 7173 683 56 BtsI GCAGTG_nn' Cuts at: 2179 2990 3012 3892 3940 5428 6222 6242 2179 Size: 811 22 880 48 1488 794 20 3849 Fragments arranged by size: 3849 1488 880 811 794 48 22 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7912 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7877 DraI TTT'AAA Cuts at: 3375 3600 5216 3375 Size: 225 1616 6071 Fragments arranged by size: 6071 1616 225 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6919 809 Size: 507 5603 1802 Fragments arranged by size: 5603 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4909 5359 6248 Size: 54 1425 50 275 18 3079 450 889 Cuts at: 6248 7877 8 Size: 1629 43 Fragments arranged by size: 3079 1629 1425 889 450 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5359 1537 Size: 275 3547 4090 Fragments arranged by size: 4090 3547 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2178 2307 2488 3062 5842 1353 Size: 825 129 181 574 2780 3423 Fragments arranged by size: 3423 2780 825 574 181 129 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6499 6873 7019 387 Size: 1064 138 4910 374 146 1280 Fragments arranged by size: 4910 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3010 3010 Size: 7912 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2046 2632 3359 4997 5975 6572 7359 Size: 393 414 586 727 1638 978 597 787 Cuts at: 7359 7627 1239 Size: 268 1524 Fragments arranged by size: 1638 1524 978 787 727 597 586 414 393 268 EcoRI G'AATT_C Cuts at: 3169 5221 5599 3169 Size: 2052 378 5482 Fragments arranged by size: 5482 2052 378 FspI TGC'GCA Cuts at: 6418 6418 Size: 7912 HaeII r_GCGC'y Cuts at: 1678 7059 1678 Size: 5381 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 3168 3200 4694 Size: 32 135 32 449 32 1115 32 1494 Cuts at: 4694 4726 6553 6585 6627 6659 1373 Size: 32 1827 32 42 32 2626 Fragments arranged by size: 2626 1827 1494 1115 449 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2088 3642 4182 4219 5256 5502 Size: 988 281 150 1554 540 37 1037 246 Cuts at: 5502 669 Size: 3079 Fragments arranged by size: 3079 1554 1037 988 540 281 246 150 37 HindIII A'AGCT_T Cuts at: 748 748 Size: 7912 HpaI GTT'AAC Cuts at: 3642 4182 5502 3642 Size: 540 1320 6052 Fragments arranged by size: 6052 1320 540 KpnI G_GTAC'C Cuts at: 5237 5237 Size: 7912 MfeI C'AATT_G Cuts at: 4786 5511 4786 Size: 725 7187 MluI A'CGCG_T Cuts at: 7788 7788 Size: 7912 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2019 2645 3258 3602 7026 7210 1305 Size: 714 626 613 344 3424 184 2007 Fragments arranged by size: 3424 2007 714 626 613 344 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6090 Fragments arranged by size: 6090 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2358 2510 3340 3393 4002 4898 5870 Size: 648 1192 152 830 53 609 896 972 Cuts at: 5870 6229 6388 518 Size: 359 159 2042 Fragments arranged by size: 2042 1192 972 896 830 648 609 359 159 152 53 MspA1I CmG'CkG Cuts at: 1606 2066 3933 5328 6005 7153 7398 1606 Size: 460 1867 1395 677 1148 245 2120 Fragments arranged by size: 2120 1867 1395 1148 677 460 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 6403 Fragments arranged by size: 6403 957 552 NdeI CA'TA_TG Cuts at: 387 2835 4895 387 Size: 2448 2060 3404 Fragments arranged by size: 3404 2448 2060 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7912 NotI GC'GGCC_GC Cuts at: 5359 5359 Size: 7912 NsiI A_TGCA'T Cuts at: 4905 4905 Size: 7912 NspI r_CATG'y Cuts at: 1132 1229 2357 2787 3491 3630 3671 3901 4907 Size: 97 1128 430 704 139 41 230 1006 Cuts at: 4907 5270 6815 1132 Size: 363 1545 2229 Fragments arranged by size: 2229 1545 1128 1006 704 430 363 230 139 97 41 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7912 PciI A'CATG_T Cuts at: 3667 6811 3667 Size: 3144 4768 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7912 PmeI GTTT'AAAC Cuts at: 5216 5216 Size: 7912 PmlI CAC'GTG Cuts at: 2508 2508 Size: 7912 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4663 4695 5981 6013 7494 7526 4663 Size: 32 1286 32 1481 32 5049 Fragments arranged by size: 5049 1481 1286 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7912 PsiI TTA'TAA Cuts at: 2676 3516 4818 5482 2676 Size: 840 1302 664 5106 Fragments arranged by size: 5106 1302 840 664 PstI C_TGCA'G Cuts at: 830 3947 4289 5264 830 Size: 3117 342 975 3478 Fragments arranged by size: 3478 3117 975 342 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7912 PvuII CAG'CTG Cuts at: 2066 3933 2066 Size: 1867 6045 SacI G_AGCT'C Cuts at: 3012 3012 Size: 7912 SalI G'TCGA_C Cuts at: 1936 5254 1936 Size: 3318 4594 SapI GCTCTTCn'nnn_ Cuts at: 2178 3062 2178 Size: 884 7028 SbfI CC_TGCA'GG Cuts at: 5264 5264 Size: 7912 ScaI AGT'ACT Cuts at: 6160 7872 6160 Size: 1712 6200 SexAI A'CCwGG_T Cuts at: 3434 3434 Size: 7912 SfcI C'TryA_G Cuts at: 826 1045 3051 3057 3943 4285 4861 5260 6395 Size: 219 2006 6 886 342 576 399 1135 Cuts at: 6395 7076 7267 826 Size: 681 191 1471 Fragments arranged by size: 2006 1471 1135 886 681 576 399 342 219 191 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7912 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7912 SmaI CCC'GGG Cuts at: 1872 2034 5239 1872 Size: 162 3205 4545 Fragments arranged by size: 4545 3205 162 SmlI C'TyrA_G Cuts at: 820 1017 1943 5195 6016 6917 7179 820 Size: 197 926 3252 821 901 262 1553 Fragments arranged by size: 3252 1553 926 901 821 262 197 SnaBI TAC'GTA Cuts at: 493 5209 5703 493 Size: 4716 494 2702 Fragments arranged by size: 4716 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7912 SphI G_CATG'C Cuts at: 1132 2357 4907 5270 1132 Size: 1225 2550 363 3774 Fragments arranged by size: 3774 2550 1225 363 SspI AAT'ATT Cuts at: 5 52 2215 5836 5 Size: 47 2163 3621 2081 Fragments arranged by size: 3621 2163 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7912 StyI C'CwwG_G Cuts at: 513 1065 2022 4747 5351 513 Size: 552 957 2725 604 3074 Fragments arranged by size: 3074 2725 957 604 552 SwaI ATTT'AAAT Cuts at: 3600 3600 Size: 7912 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2500 2864 3167 5105 5939 5956 6109 Size: 1255 920 364 303 1938 834 17 153 Cuts at: 6109 6294 6739 325 Size: 185 445 1498 Fragments arranged by size: 1938 1498 1255 920 834 445 364 303 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4512 6158 7870 Size: 274 80 33 51 1443 2535 1646 1712 Cuts at: 7870 96 Size: 138 Fragments arranged by size: 2535 1712 1646 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7872 7904 7872 Size: 32 7880 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7912 XbaI T'CTAG_A Cuts at: 4915 5248 4915 Size: 333 7579 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4184 5161 4184 Size: 977 6935 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7912 XmaI C'CCGG_G Cuts at: 1870 2032 5237 1870 Size: 162 3205 4545 Fragments arranged by size: 4545 3205 162 XmnI GAAnn'nnTTC Cuts at: 2382 2597 2852 4065 6041 2382 Size: 215 255 1213 1976 4253 Fragments arranged by size: 4253 1976 1213 255 215 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5720 276 Size: 53 83 186 936 4186 2468 Fragments arranged by size: 4186 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI