(Circular) (Six-base) MAPSORT of: pFN21ASDA0573.seq Check: 4439 from: 1 to: 5947 pFN21ASDA0573 5947 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3757 278 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3212 3290 1937 Size: 1275 78 4594 Fragments arranged by size: 4594 1275 78 Acc65I G'GTAC_C Cuts at: 3268 3268 Size: 5947 AclI AA'CG_TT Cuts at: 1563 4074 4447 1563 Size: 2511 373 3063 Fragments arranged by size: 3063 2511 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4010 5394 5662 1632 Size: 2378 1384 268 1917 Fragments arranged by size: 2378 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5750 AflIII A'CryG_T Cuts at: 1203 4846 5823 1203 Size: 3643 977 1327 Fragments arranged by size: 3643 1327 977 AgeI A'CCGG_T Cuts at: 4778 4778 Size: 5947 AhdI GACnn_n'nnGTC Cuts at: 4676 4676 Size: 5947 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5947 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5947 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2011 2043 2011 Size: 32 5915 AlwNI CAG_nnn'CTG Cuts at: 3690 5262 3690 Size: 1572 4375 ApaLI G'TGCA_C Cuts at: 4004 5160 4004 Size: 1156 4791 ApoI r'AATT_y Cuts at: 1493 2145 2176 2255 2460 2707 2930 3098 3256 Size: 652 31 79 205 247 223 168 158 Cuts at: 3256 3482 3634 5743 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 652 247 226 223 205 168 158 152 79 31 AseI AT'TA_AT Cuts at: 160 1032 2882 3038 4501 160 Size: 872 1850 156 1463 1606 Fragments arranged by size: 1850 1606 1463 872 156 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 AvaI C'yCGr_G Cuts at: 1870 1943 3272 1870 Size: 73 1329 4545 Fragments arranged by size: 4545 1329 73 BamHI G'GATC_C Cuts at: 3277 3644 3277 Size: 367 5580 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3268 4723 618 Size: 325 217 627 1481 1455 1842 Fragments arranged by size: 1842 1481 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3179 3266 1391 Size: 1788 87 4072 Fragments arranged by size: 4072 1788 87 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2029 928 Size: 971 130 4846 Fragments arranged by size: 4846 971 130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2557 2591 4138 4172 1903 Size: 34 620 34 1547 34 3678 Fragments arranged by size: 3678 1547 620 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3839 5049 3839 Size: 1210 4737 BclI T'GATC_A Cuts at: 1548 2299 1548 Size: 751 5196 BfrBI ATG'CAT Cuts at: 2026 2026 Size: 5947 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4558 136 Size: 107 122 71 631 605 2886 1525 Fragments arranged by size: 2886 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5942 5942 Size: 5947 BlpI GC'TnA_GC Cuts at: 3364 3364 Size: 5947 Bme1580I G_kGCm'C Cuts at: 1790 4008 5164 1790 Size: 2218 1156 2573 Fragments arranged by size: 2573 2218 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5947 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3192 3678 4636 Size: 416 53 83 399 24 1775 486 958 Cuts at: 4636 442 Size: 1753 Fragments arranged by size: 1775 1753 958 486 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5947 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2949 4607 1239 Size: 1710 1658 2579 Fragments arranged by size: 2579 1710 1658 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3038 4072 4937 5235 3038 Size: 1034 865 298 3750 Fragments arranged by size: 3750 1034 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4610 882 Size: 617 3111 2219 Fragments arranged by size: 3111 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3244 3738 493 Size: 711 2040 494 2702 Fragments arranged by size: 2702 2040 711 494 BsaBI GATnn'nnATC Cuts at: 2298 5941 2298 Size: 3643 2304 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3754 4136 4771 275 Size: 53 83 186 936 2221 382 635 1451 Fragments arranged by size: 2221 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2623 2677 4380 4778 5052 5199 5610 2623 Size: 54 1703 398 274 147 411 2960 Fragments arranged by size: 2960 1703 411 398 274 147 54 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5722 Fragments arranged by size: 5722 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5947 BseYI C'CCAG_C Cuts at: 5150 5646 5150 Size: 496 5451 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2933 3074 1163 Size: 573 1197 141 4036 Fragments arranged by size: 4036 1197 573 141 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3397 4158 5186 1540 Size: 275 186 1396 761 1028 2301 Fragments arranged by size: 2301 1396 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3179 4008 4093 5164 3179 Size: 829 85 1071 3962 Fragments arranged by size: 3962 1071 829 85 BsmI GAATG_Cn' Cuts at: 2668 3458 3551 2668 Size: 790 93 5064 Fragments arranged by size: 5064 790 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3179 3266 4008 4093 5164 1391 Size: 399 1389 87 742 85 1071 2174 Fragments arranged by size: 2174 1389 1071 742 399 87 85 BspEI T'CCGG_A Cuts at: 2677 5610 2677 Size: 2933 3014 BspHI T'CATG_A Cuts at: 3836 5566 3836 Size: 1730 4217 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3302 844 Size: 2458 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3834 1394 Size: 429 2011 3507 Fragments arranged by size: 3507 2011 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4442 4616 65 Size: 1183 3194 174 1396 Fragments arranged by size: 3194 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4591 4778 1700 Size: 110 2781 187 2869 Fragments arranged by size: 2869 2781 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5947 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5947 BssSI C'ACGA_G Cuts at: 4007 5019 4007 Size: 1012 4935 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5947 BstBI TT'CG_AA Cuts at: 2007 4751 2007 Size: 2744 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5947 BstYI r'GATC_y Cuts at: 1972 3277 3644 4029 4046 5498 5584 5596 5942 Size: 1305 367 385 17 1452 86 12 346 Cuts at: 5942 1972 Size: 1977 Fragments arranged by size: 1977 1452 1305 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3213 3213 Size: 5947 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5395 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5208 Fragments arranged by size: 5208 683 56 BtsI GCAGTG_nn' Cuts at: 3463 4257 4277 3463 Size: 794 20 5133 Fragments arranged by size: 5133 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5947 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5912 DraI TTT'AAA Cuts at: 2151 3251 2151 Size: 1100 4847 DraIII CAC_nnn'GTG Cuts at: 3200 3200 Size: 5947 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4954 809 Size: 507 3638 1802 Fragments arranged by size: 3638 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3394 4283 5912 Size: 54 1425 50 275 18 1564 889 1629 Cuts at: 5912 8 Size: 43 Fragments arranged by size: 1629 1564 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3394 1537 Size: 275 1582 4090 Fragments arranged by size: 4090 1582 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2375 2949 3877 1353 Size: 1022 574 928 3423 Fragments arranged by size: 3423 1022 928 574 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4534 4908 5054 387 Size: 1064 138 2945 374 146 1280 Fragments arranged by size: 2945 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3177 3177 Size: 5947 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2949 4010 4607 5394 5662 1239 Size: 393 1317 1061 597 787 268 1524 Fragments arranged by size: 1524 1317 1061 787 597 393 268 EcoRI G'AATT_C Cuts at: 3098 3256 3634 3098 Size: 158 378 5411 Fragments arranged by size: 5411 378 158 FspI TGC'GCA Cuts at: 4453 4453 Size: 5947 HaeII r_GCGC'y Cuts at: 1678 5094 1678 Size: 3416 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2200 2232 2291 2323 4588 Size: 32 135 32 628 32 59 32 2265 Cuts at: 4588 4620 4662 4694 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2265 628 135 59 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3291 3537 669 Size: 988 281 1353 246 3079 Fragments arranged by size: 3079 1353 988 281 246 HindIII A'AGCT_T Cuts at: 748 2232 748 Size: 1484 4463 HpaI GTT'AAC Cuts at: 3537 3537 Size: 5947 KpnI G_GTAC'C Cuts at: 3272 3272 Size: 5947 MfeI C'AATT_G Cuts at: 2725 3546 2725 Size: 821 5126 MluI A'CGCG_T Cuts at: 5823 5823 Size: 5947 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5061 5245 1305 Size: 3756 184 2007 Fragments arranged by size: 3756 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4125 Fragments arranged by size: 4125 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3009 3905 4264 4423 518 Size: 648 1843 896 359 159 2042 Fragments arranged by size: 2042 1843 896 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3363 4040 5188 5433 1606 Size: 1757 677 1148 245 2120 Fragments arranged by size: 2120 1757 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5395 NdeI CA'TA_TG Cuts at: 387 3006 387 Size: 2619 3328 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5947 NotI GC'GGCC_GC Cuts at: 3394 3394 Size: 5947 NsiI A_TGCA'T Cuts at: 2028 2028 Size: 5947 NspI r_CATG'y Cuts at: 1132 1229 2918 3305 4850 1132 Size: 97 1689 387 1545 2229 Fragments arranged by size: 2229 1689 1545 387 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5947 PciI A'CATG_T Cuts at: 4846 4846 Size: 5947 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5947 PfoI T'CCnGG_A Cuts at: 3043 3043 Size: 5947 PmeI GTTT'AAAC Cuts at: 3251 3251 Size: 5947 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4016 4048 5529 5561 4016 Size: 32 1481 32 4402 Fragments arranged by size: 4402 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2193 1613 Size: 580 5367 PsiI TTA'TAA Cuts at: 3517 3517 Size: 5947 PstI C_TGCA'G Cuts at: 830 3299 830 Size: 2469 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5947 SacI G_AGCT'C Cuts at: 3179 3179 Size: 5947 SalI G'TCGA_C Cuts at: 1936 3289 1936 Size: 1353 4594 SbfI CC_TGCA'GG Cuts at: 3299 3299 Size: 5947 ScaI AGT'ACT Cuts at: 4195 5907 4195 Size: 1712 4235 SfcI C'TryA_G Cuts at: 826 1045 2545 3295 4430 5111 5302 826 Size: 219 1500 750 1135 681 191 1471 Fragments arranged by size: 1500 1471 1135 750 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5947 SmaI CCC'GGG Cuts at: 1872 3274 1872 Size: 1402 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3053 4051 4952 5214 820 Size: 197 926 1110 998 901 262 1553 Fragments arranged by size: 1553 1110 998 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3244 3738 493 Size: 2751 494 2702 Fragments arranged by size: 2751 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5947 SphI G_CATG'C Cuts at: 1132 3305 1132 Size: 2173 3774 SspI AAT'ATT Cuts at: 5 52 2572 2856 3871 5 Size: 47 2520 284 1015 2081 Fragments arranged by size: 2520 2081 1015 284 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5947 StyI C'CwwG_G Cuts at: 513 1065 3386 513 Size: 552 2321 3074 Fragments arranged by size: 3074 2321 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3211 3974 3991 4144 4329 4774 325 Size: 1255 1631 763 17 153 185 445 1498 Fragments arranged by size: 1631 1498 1255 763 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2688 4193 5905 Size: 274 80 33 51 1443 711 1505 1712 Cuts at: 5905 96 Size: 138 Fragments arranged by size: 1712 1505 1443 711 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5907 5939 5907 Size: 32 5915 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5947 XbaI T'CTAG_A Cuts at: 3283 3283 Size: 5947 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5947 XmaI C'CCGG_G Cuts at: 1870 3272 1870 Size: 1402 4545 XmnI GAAnn'nnTTC Cuts at: 2256 4076 2256 Size: 1820 4127 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3755 276 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI