Restriction map for
pFN21ASDA0554
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstPI BstXI BstYI Bsu36I
CelII ClaI CpoI DraI DraII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FseI FspI HaeII
Hin1I HpaI KasI KpnI KspI
MflI MluI MroI MslI NarI
NciI NcoI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StyI SwaI Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AlwNI 319 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AscI 1476 GGCGCGCC GG^CGCGCC
Asp700I 1700 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 16 GAGCTC G[AT]GC[AT]^C
BalI 1604 TGGCCA TGG^CCA
BanI 10 GGCACC G^G[CT][GA]CC
BbvCI 1438 GCTGAGG CCTCAGC(-5/-2)
BclI 777 TGATCA T^GATCA
BfaI 407 CTAG C^TAG
BsaBI 723 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1194 ACCGGA [AT]^CCGG[AT]
BsiHKAI 16 GAGCTC G[AT]GC[AT]^C
BsmI 1769 GCATTC GAATGC(1/-1)
BspHI 1121 TCATGA T^CATGA
BspMI 717 GCAGGT ACCTGC(4/8)
BsrBI 1488 GAGCGG CCGCTC(-3/-3)
BssHII 1477 GCGCGC G^CGCGC
Bst1107I 198 GTATAC GTA^TAC
BstZ17I 198 GTATAC GTA^TAC
DraIII 51 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 481 GACATCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1607 CCACGG C^C[GA][CT]GG
EarI 1789 GAAGAG CTCTTC(1/4)
EcoNI 1648 CCTGCCATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 1766 CCCGC CCCGC(4/6)
FbaI 777 TGATCA T^GATCA
HgaI 475 GACGC GACGC(5/10)
HincII 1522 GTCAAC GT[CT]^[GA]AC
HindII 1522 GTCAAC GT[CT]^[GA]AC
Ksp632I 1789 GAAGAG CTCTTC(1/4)
MaeI 407 CTAG C^TAG
MamI 723 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 773 CAATTG C^AATTG
MluNI 1604 TGGCCA TGG^CCA
MscI 1604 TGGCCA TGG^CCA
MspA1I 796 CAGCTG C[AC]G^C[GT]G
MunI 773 CAATTG C^AATTG
PflMI 727 CCAATCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1240 CTGCAG CTGCA^G
PvuII 796 CAGCTG CAG^CTG
RcaI 1121 TCATGA T^CATGA
SacI 16 GAGCTC GAGCT^C
ScaI 458 AGTACT AGT^ACT
SfcI 1240 CTGCAG C^T[GA][CT]AG
SspI 608 AATATT AAT^ATT
StuI 1427 AGGCCT AGG^CCT
Van91I 727 CCAATCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1700 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 198 GTATAC GT^[AC][GT]AC
1321 GTCTAC
AccII 427 CGCG CG^CG
1478 CGCG
AvaII 1539 GGACC G^G[AT]CC
1763 GGACC
BbsI 693 GAAGAC GAAGAC(2/6)
1744 GAAGAC
BglI 1429 GCCTGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1550 GCCCAGATGGC
BpmI 580 CTCCAG CTGGAG(16/14)
836 CTGGAG
BpuAI 693 GAAGAC GAAGAC(2/6)
1744 GAAGAC
BsaI 1346 GAGACC GGTCTC(1/5)
1411 GAGACC
BsaJI 1535 CCCAGG C^C[ACGT][ACGT]GG
1607 CCACGG
BsrFI 1451 GCCGGC [GA]^CCGG[CT]
1481 GCCGGC
BsrI 1071 CCAGT ACTGG(1/-1)
1355 CCAGT
BstUI 427 CGCG CG^CG
1478 CGCG
Cfr10I 1451 GCCGGC [GA]^CCGG[CT]
1481 GCCGGC
MseI 1000 TTAA T^TAA
1261 TTAA
MvnI 427 CGCG CG^CG
1478 CGCG
NaeI 1451 GCCGGC GCC^GGC
1481 GCCGGC
NdeI 698 CATATG CA^TATG
1811 CATATG
NgoMIV 1451 GCCGGC G^CCGGC
1481 GCCGGC
PleI 685 GAGTC GAGTC(4/5)
1578 GAGTC
SfaNI 1297 GATGC GCATC(5/9)
283 GCATC
SmlI 73 CTTGAG C^T[CT][GA]AG
1177 CTCAAG
Tru9I 1000 TTAA T^TAA
1261 TTAA
Tsp45I 490 GTCAC ^GT[GC]AC
536 GTCAC
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanII BbvI BmyI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TaqI TfiI TseI Tsp509I
TspRI TthHB8I