(Circular) (Six-base) MAPSORT of: pFN21ASDA0554.seq Check: 4978 from: 1 to: 6580 pFN21ASDA0554 6580 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2731 2731 Size: 6580 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4390 278 Size: 53 83 186 936 2854 2468 Fragments arranged by size: 2854 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2222 3345 3923 1937 Size: 285 1123 578 4594 Fragments arranged by size: 4594 1123 578 285 Acc65I G'GTAC_C Cuts at: 3901 3901 Size: 6580 AclI AA'CG_TT Cuts at: 1563 4707 5080 1563 Size: 3144 373 3063 Fragments arranged by size: 3144 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3157 4643 6027 6295 1632 Size: 1525 1486 1384 268 1917 Fragments arranged by size: 1917 1525 1486 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6383 AflIII A'CryG_T Cuts at: 1203 5479 6456 1203 Size: 4276 977 1327 Fragments arranged by size: 4276 1327 977 AgeI A'CCGG_T Cuts at: 5411 5411 Size: 6580 AhdI GACnn_n'nnGTC Cuts at: 5309 5309 Size: 6580 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6580 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6580 AlwNI CAG_nnn'CTG Cuts at: 2347 4323 5895 2347 Size: 1976 1572 3032 Fragments arranged by size: 3032 1976 1572 ApaLI G'TGCA_C Cuts at: 4637 5793 4637 Size: 1156 5424 ApoI r'AATT_y Cuts at: 1493 2609 2870 2926 2957 3294 3443 3889 4115 Size: 1116 261 56 31 337 149 446 226 Cuts at: 4115 4267 6376 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1116 446 337 261 226 152 149 56 31 AscI GG'CGCG_CC Cuts at: 3500 3500 Size: 6580 AseI AT'TA_AT Cuts at: 160 1032 5134 160 Size: 872 4102 1606 Fragments arranged by size: 4102 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 AvaI C'yCGr_G Cuts at: 1870 1943 3905 1870 Size: 73 1962 4545 Fragments arranged by size: 4545 1962 73 BamHI G'GATC_C Cuts at: 3910 4277 3910 Size: 367 6213 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 3901 5356 618 Size: 325 217 627 246 1868 1455 1842 Fragments arranged by size: 1868 1842 1455 627 325 246 217 BanII G_rGCy'C Cuts at: 1391 2043 3575 3666 3899 1391 Size: 652 1532 91 233 4072 Fragments arranged by size: 4072 1532 652 233 91 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2723 3774 928 Size: 971 824 1051 3734 Fragments arranged by size: 3734 1051 971 824 BbvCI CC'TCA_GC Cuts at: 3462 3462 Size: 6580 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4771 4805 1903 Size: 34 2834 34 3678 Fragments arranged by size: 3678 2834 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4472 5682 4472 Size: 1210 5370 BclI T'GATC_A Cuts at: 1548 2800 1548 Size: 1252 5328 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3458 3579 5191 Size: 107 122 71 631 605 1786 121 1612 Cuts at: 5191 136 Size: 1525 Fragments arranged by size: 1786 1612 1525 631 605 122 121 107 71 BglII A'GATC_T Cuts at: 6575 6575 Size: 6580 BlpI GC'TnA_GC Cuts at: 3997 3997 Size: 6580 Bme1580I G_kGCm'C Cuts at: 1790 2036 4641 5797 1790 Size: 246 2605 1156 2573 Fragments arranged by size: 2605 2573 1156 246 BmgBI CAC'GTC Cuts at: 1318 3684 1318 Size: 2366 4214 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3088 4311 5269 Size: 416 53 83 399 24 1671 1223 958 Cuts at: 5269 442 Size: 1753 Fragments arranged by size: 1753 1671 1223 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6580 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2588 2880 5240 1239 Size: 1349 292 2360 2579 Fragments arranged by size: 2579 2360 1349 292 Bpu10I CC'TnA_GC Cuts at: 3462 3462 Size: 6580 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2117 3185 4705 5570 5868 2117 Size: 1068 1520 865 298 2829 Fragments arranged by size: 2829 1520 1068 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3363 3428 5243 882 Size: 617 1864 65 1815 2219 Fragments arranged by size: 2219 1864 1815 617 65 BsaAI yAC'GTr Cuts at: 493 1204 3877 4371 493 Size: 711 2673 494 2702 Fragments arranged by size: 2702 2673 711 494 BsaBI GATnn'nnATC Cuts at: 2750 6574 2750 Size: 3824 2756 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4387 4769 5404 275 Size: 53 83 186 936 2854 382 635 1451 Fragments arranged by size: 2854 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3217 5013 5411 5685 5832 6243 3217 Size: 1796 398 274 147 411 3554 Fragments arranged by size: 3554 1796 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6355 Fragments arranged by size: 6355 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2949 3608 3674 1186 Size: 1763 659 66 4092 Fragments arranged by size: 4092 1763 659 66 BseYI C'CCAG_C Cuts at: 2028 3205 3373 3481 5783 6279 2028 Size: 1177 168 108 2302 496 2329 Fragments arranged by size: 2329 2302 1177 496 168 108 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6007 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4030 4791 5819 1540 Size: 275 186 2029 761 1028 2301 Fragments arranged by size: 2301 2029 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2043 4641 4726 5797 2043 Size: 2598 85 1071 2826 Fragments arranged by size: 2826 2598 1071 85 BsmI GAATG_Cn' Cuts at: 3792 4091 4184 3792 Size: 299 93 6188 Fragments arranged by size: 6188 299 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2043 3575 3666 3899 4641 4726 Size: 399 246 7 1532 91 233 742 85 Cuts at: 4726 5797 1391 Size: 1071 2174 Fragments arranged by size: 2174 1532 1071 742 399 246 233 91 85 7 BspEI T'CCGG_A Cuts at: 6243 6243 Size: 6580 BspHI T'CATG_A Cuts at: 3144 4469 6199 3144 Size: 1325 1730 3525 Fragments arranged by size: 3525 1730 1325 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2731 3935 844 Size: 1887 1204 3489 Fragments arranged by size: 3489 1887 1204 BsrBI CCG'CTC Cuts at: 1394 1823 3513 4467 1394 Size: 429 1690 954 3507 Fragments arranged by size: 3507 1690 954 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5075 5249 65 Size: 1183 3827 174 1396 Fragments arranged by size: 3827 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3474 3504 5224 5411 1700 Size: 110 1664 30 1720 187 2869 Fragments arranged by size: 2869 1720 1664 187 110 30 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6580 BssHII G'CGCG_C Cuts at: 1925 3500 1925 Size: 1575 5005 BssSI C'ACGA_G Cuts at: 4640 5652 4640 Size: 1012 5568 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6580 BstBI TT'CG_AA Cuts at: 2007 5384 2007 Size: 3377 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6580 BstYI r'GATC_y Cuts at: 1972 3910 4277 4662 4679 6131 6217 6229 6575 Size: 1938 367 385 17 1452 86 12 346 Cuts at: 6575 1972 Size: 1977 Fragments arranged by size: 1977 1938 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2223 2223 Size: 6580 BtgI C'CryG_G Cuts at: 513 1065 3630 513 Size: 552 2565 3463 Fragments arranged by size: 3463 2565 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3792 487 Size: 683 56 2566 3275 Fragments arranged by size: 3275 2566 683 56 BtsI GCAGTG_nn' Cuts at: 3166 4096 4890 4910 3166 Size: 930 794 20 4836 Fragments arranged by size: 4836 930 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6580 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2944 2979 3619 3654 529 Size: 35 2380 35 640 35 3455 Fragments arranged by size: 3455 2380 640 35 35 35 DraI TTT'AAA Cuts at: 3884 3884 Size: 6580 DraIII CAC_nnn'GTG Cuts at: 2079 2079 Size: 6580 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2510 5587 809 Size: 507 1194 3077 1802 Fragments arranged by size: 3077 1802 1194 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2380 2987 3627 Size: 54 1425 50 275 18 550 607 640 Cuts at: 3627 4027 4916 6545 8 Size: 400 889 1629 43 Fragments arranged by size: 1629 1425 889 640 607 550 400 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4027 1537 Size: 275 2215 4090 Fragments arranged by size: 4090 2215 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3807 4510 1353 Size: 2454 703 3423 Fragments arranged by size: 3423 2454 703 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2480 5167 5541 5687 387 Size: 1064 138 891 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 891 374 146 138 EcoICRI GAG'CTC Cuts at: 2041 2041 Size: 6580 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2588 2880 3157 4643 5240 6027 6295 Size: 393 956 292 277 1486 597 787 268 Cuts at: 6295 1239 Size: 1524 Fragments arranged by size: 1524 1486 956 787 597 393 292 277 268 EcoNI CCTnn'n_nnAGG Cuts at: 3675 3675 Size: 6580 EcoRI G'AATT_C Cuts at: 3889 4267 3889 Size: 378 6202 FspI TGC'GCA Cuts at: 5086 5086 Size: 6580 HaeII r_GCGC'y Cuts at: 1678 5727 1678 Size: 4049 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2348 2380 2579 2611 5221 Size: 32 135 32 776 32 199 32 2610 Cuts at: 5221 5253 5295 5327 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2610 776 199 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3547 3924 4170 669 Size: 988 281 1609 377 246 3079 Fragments arranged by size: 3079 1609 988 377 281 246 HindIII A'AGCT_T Cuts at: 748 2235 2832 3008 748 Size: 1487 597 176 4320 Fragments arranged by size: 4320 1487 597 176 HpaI GTT'AAC Cuts at: 4170 4170 Size: 6580 KpnI G_GTAC'C Cuts at: 3905 3905 Size: 6580 MfeI C'AATT_G Cuts at: 2796 4179 2796 Size: 1383 5197 MluI A'CGCG_T Cuts at: 6456 6456 Size: 6580 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2271 5694 5878 1305 Size: 966 3423 184 2007 Fragments arranged by size: 3423 2007 966 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3629 10 Size: 54 1425 343 1797 2961 Fragments arranged by size: 2961 1797 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4538 4897 5056 518 Size: 648 3372 359 159 2042 Fragments arranged by size: 3372 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2821 3996 4673 5821 6066 1606 Size: 1215 1175 677 1148 245 2120 Fragments arranged by size: 2120 1215 1175 1148 677 245 NaeI GCC'GGC Cuts at: 3476 3506 3476 Size: 30 6550 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6028 NdeI CA'TA_TG Cuts at: 387 2722 3835 387 Size: 2335 1113 3132 Fragments arranged by size: 3132 2335 1113 NgoMIV G'CCGG_C Cuts at: 3474 3504 3474 Size: 30 6550 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6580 NotI GC'GGCC_GC Cuts at: 4027 4027 Size: 6580 NspI r_CATG'y Cuts at: 1132 1229 3938 5483 1132 Size: 97 2709 1545 2229 Fragments arranged by size: 2709 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6580 PciI A'CATG_T Cuts at: 5479 5479 Size: 6580 PflMI CCAn_nnn'nTGG Cuts at: 1108 2028 2756 1108 Size: 920 728 4932 Fragments arranged by size: 4932 920 728 PfoI T'CCnGG_A Cuts at: 2857 2857 Size: 6580 PmeI GTTT'AAAC Cuts at: 3884 3884 Size: 6580 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4649 4681 6162 6194 4649 Size: 32 1481 32 5035 Fragments arranged by size: 5035 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6580 PsiI TTA'TAA Cuts at: 2838 4150 2838 Size: 1312 5268 PstI C_TGCA'G Cuts at: 830 3267 3932 830 Size: 2437 665 3478 Fragments arranged by size: 3478 2437 665 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6580 PvuII CAG'CTG Cuts at: 2821 2821 Size: 6580 SacI G_AGCT'C Cuts at: 2043 2043 Size: 6580 SalI G'TCGA_C Cuts at: 1936 3922 1936 Size: 1986 4594 SbfI CC_TGCA'GG Cuts at: 3932 3932 Size: 6580 ScaI AGT'ACT Cuts at: 2483 4828 6540 2483 Size: 2345 1712 2523 Fragments arranged by size: 2523 2345 1712 SfcI C'TryA_G Cuts at: 826 1045 3263 3928 5063 5744 5935 826 Size: 219 2218 665 1135 681 191 1471 Fragments arranged by size: 2218 1471 1135 681 665 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6580 SmaI CCC'GGG Cuts at: 1872 3907 1872 Size: 2035 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2096 3200 4684 5585 5847 820 Size: 197 926 153 1104 1484 901 262 1553 Fragments arranged by size: 1553 1484 1104 926 901 262 197 153 SnaBI TAC'GTA Cuts at: 493 3877 4371 493 Size: 3384 494 2702 Fragments arranged by size: 3384 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6580 SphI G_CATG'C Cuts at: 1132 3938 1132 Size: 2806 3774 SspI AAT'ATT Cuts at: 5 52 2633 4504 5 Size: 47 2581 1871 2081 Fragments arranged by size: 2581 2081 1871 47 StuI AGG'CCT Cuts at: 1517 3452 1517 Size: 1935 4645 StyI C'CwwG_G Cuts at: 513 1065 4019 513 Size: 552 2954 3074 Fragments arranged by size: 3074 2954 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3197 3553 4607 4624 4777 4962 5407 Size: 1255 1617 356 1054 17 153 185 445 Cuts at: 5407 325 Size: 1498 Fragments arranged by size: 1617 1498 1255 1054 445 356 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2481 4826 6538 Size: 274 80 33 51 1443 504 2345 1712 Cuts at: 6538 96 Size: 138 Fragments arranged by size: 2345 1712 1443 504 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6540 6572 6540 Size: 32 6548 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6580 XbaI T'CTAG_A Cuts at: 3916 3916 Size: 6580 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6580 XmaI C'CCGG_G Cuts at: 1870 3905 1870 Size: 2035 4545 XmnI GAAnn'nnTTC Cuts at: 3727 4709 3727 Size: 982 5598 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4388 276 Size: 53 83 186 936 2854 2468 Fragments arranged by size: 2854 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI Bsu36I EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI