Restriction map for
pFN21ASDA0554
(Linear) (Six-base) MAPSORT of: KIAA0554.seq Check: 3387 from: 1 to: 1851
KIAA0554.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 708 1851
Size: 708 1143
AccI GT'mk_AC
Cuts at: 0 199 1322 1851
Size: 199 1123 529
Fragments arranged by size:
1123 529 199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1134 1851
Size: 1134 717
AlwNI CAG_nnn'CTG
Cuts at: 0 324 1851
Size: 324 1527
ApoI r'AATT_y
Cuts at: 0 586 847 903 934 1271 1420 1851
Size: 586 261 56 31 337 149 431
Fragments arranged by size:
586 431 337 261 149 56 31
AscI GG'CGCG_CC
Cuts at: 0 1477 1851
Size: 1477 374
BanI G'GyrC_C
Cuts at: 0 10 1851
Size: 10 1841
BanII G_rGCy'C
Cuts at: 0 20 1552 1643 1851
Size: 20 1532 91 208
Fragments arranged by size:
1532 208 91 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 700 1751 1851
Size: 700 1051 100
Fragments arranged by size:
1051 700 100
BbvCI CC'TCA_GC
Cuts at: 0 1439 1851
Size: 1439 412
BclI T'GATC_A
Cuts at: 0 777 1851
Size: 777 1074
BglI GCCn_nnn'nGGC
Cuts at: 0 1435 1556 1851
Size: 1435 121 295
Fragments arranged by size:
1435 295 121
Bme1580I G_kGCm'C
Cuts at: 0 13 1851
Size: 13 1838
BmgBI CAC'GTC
Cuts at: 0 1661 1851
Size: 1661 190
BmrI ACTGGGnnnn_n'
Cuts at: 0 1065 1851
Size: 1065 786
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 857 1851
Size: 565 292 994
Fragments arranged by size:
994 565 292
Bpu10I CC'TnA_GC
Cuts at: 0 1439 1851
Size: 1439 412
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 1162 1851
Size: 94 1068 689
Fragments arranged by size:
1068 689 94
BsaI GGTCTCn'nnnn_
Cuts at: 0 1340 1405 1851
Size: 1340 65 446
Fragments arranged by size:
1340 446 65
BsaBI GATnn'nnATC
Cuts at: 0 727 1851
Size: 727 1124
BsaWI w'CCGG_w
Cuts at: 0 1194 1851
Size: 1194 657
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 926 1585 1651 1851
Size: 926 659 66 200
Fragments arranged by size:
926 659 200 66
BseYI C'CCAG_C
Cuts at: 0 5 1182 1350 1458 1851
Size: 5 1177 168 108 393
Fragments arranged by size:
1177 393 168 108 5
BsiHKAI G_wGCw'C
Cuts at: 0 20 1851
Size: 20 1831
BsmI GAATG_Cn'
Cuts at: 0 1769 1851
Size: 1769 82
Bsp1286I G_dGCh'C
Cuts at: 0 13 20 1552 1643 1851
Size: 13 7 1532 91 208
Fragments arranged by size:
1532 208 91 13 7
BspHI T'CATG_A
Cuts at: 0 1121 1851
Size: 1121 730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 708 1851
Size: 708 1143
BsrBI CCG'CTC
Cuts at: 0 1490 1851
Size: 1490 361
BsrFI r'CCGG_y
Cuts at: 0 1451 1481 1851
Size: 1451 30 370
Fragments arranged by size:
1451 370 30
BssHII G'CGCG_C
Cuts at: 0 1477 1851
Size: 1477 374
BstZ17I GTA'TAC
Cuts at: 0 200 1851
Size: 200 1651
BtgI C'CryG_G
Cuts at: 0 1607 1851
Size: 1607 244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1769 1851
Size: 1769 82
BtsI GCAGTG_nn'
Cuts at: 0 1143 1851
Size: 1143 708
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 921 956 1596 1631 1851
Size: 921 35 640 35 220
Fragments arranged by size:
921 640 220 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 56 1851
Size: 56 1795
DrdI GACnn_nn'nnGTC
Cuts at: 0 487 1851
Size: 487 1364
EaeI y'GGCC_r
Cuts at: 0 357 964 1604 1851
Size: 357 607 640 247
Fragments arranged by size:
640 607 357 247
EarI CTCTTCn'nnn_
Cuts at: 0 1784 1851
Size: 1784 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 457 1851
Size: 457 1394
EcoICRI GAG'CTC
Cuts at: 0 18 1851
Size: 18 1833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 857 1134 1851
Size: 565 292 277 717
Fragments arranged by size:
717 565 292 277
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1652 1851
Size: 1652 199
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 325 357 556 588 1851
Size: 325 32 199 32 1263
Fragments arranged by size:
1263 325 199 32 32
HincII GTy'rAC
Cuts at: 0 1524 1851
Size: 1524 327
HindIII A'AGCT_T
Cuts at: 0 212 809 985 1851
Size: 212 597 176 866
Fragments arranged by size:
866 597 212 176
MfeI C'AATT_G
Cuts at: 0 773 1851
Size: 773 1078
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 1851
Size: 248 1603
MscI TGG'CCA
Cuts at: 0 1606 1851
Size: 1606 245
MspA1I CmG'CkG
Cuts at: 0 798 1851
Size: 798 1053
NaeI GCC'GGC
Cuts at: 0 1453 1483 1851
Size: 1453 30 368
Fragments arranged by size:
1453 368 30
NdeI CA'TA_TG
Cuts at: 0 699 1812 1851
Size: 699 1113 39
Fragments arranged by size:
1113 699 39
NgoMIV G'CCGG_C
Cuts at: 0 1451 1481 1851
Size: 1451 30 370
Fragments arranged by size:
1451 370 30
PflMI CCAn_nnn'nTGG
Cuts at: 0 733 1851
Size: 733 1118
PfoI T'CCnGG_A
Cuts at: 0 834 1851
Size: 834 1017
PsiI TTA'TAA
Cuts at: 0 815 1851
Size: 815 1036
PstI C_TGCA'G
Cuts at: 0 1244 1851
Size: 1244 607
PvuII CAG'CTG
Cuts at: 0 798 1851
Size: 798 1053
SacI G_AGCT'C
Cuts at: 0 20 1851
Size: 20 1831
ScaI AGT'ACT
Cuts at: 0 460 1851
Size: 460 1391
SfcI C'TryA_G
Cuts at: 0 1240 1851
Size: 1240 611
SmlI C'TyrA_G
Cuts at: 0 73 1177 1851
Size: 73 1104 674
Fragments arranged by size:
1104 674 73
SspI AAT'ATT
Cuts at: 0 610 1851
Size: 610 1241
StuI AGG'CCT
Cuts at: 0 1429 1851
Size: 1429 422
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1174 1530 1851
Size: 1174 356 321
Fragments arranged by size:
1174 356 321
TatI w'GTAC_w
Cuts at: 0 458 1851
Size: 458 1393
XmnI GAAnn'nnTTC
Cuts at: 0 1704 1851
Size: 1704 147
Enzymes that do cut:
AarI AccI AcuI AlwNI ApoI AscI BanI BanII
BbsI BbvCI BclI BglI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaBI BsaWI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BstZ17I
BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI Hin4I HincII HindIII MfeI
MmeI MscI MspA1I NaeI NdeI NgoMIV PflMI PfoI
PsiI PstI PvuII SacI ScaI SfcI SmlI SspI
StuI TaqII TatI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BciVI BfrBI BglII
BlpI BmtI BplI BsaAI BsaHI BsaXI BsgI BsiEI
BsiWI BsmBI BspEI BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I ClaI DraI EagI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII HpaI
KasI KpnI MluI MslI NarI NcoI NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StyI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI