(Circular) (Six-base) MAPSORT of: pFN21ASDA0552.seq Check: 7756 from: 1 to: 6748 pFN21ASDA0552 6748 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3142 3187 3635 3142 Size: 45 448 6255 Fragments arranged by size: 6255 448 45 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4558 278 Size: 53 83 186 936 3022 2468 Fragments arranged by size: 3022 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4091 1937 Size: 2154 4594 Acc65I G'GTAC_C Cuts at: 2189 4069 2189 Size: 1880 4868 AclI AA'CG_TT Cuts at: 1563 4875 5248 1563 Size: 3312 373 3063 Fragments arranged by size: 3312 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3398 3410 3499 4811 6195 6463 1632 Size: 1766 12 89 1312 1384 268 1917 Fragments arranged by size: 1917 1766 1384 1312 268 89 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6551 AflIII A'CryG_T Cuts at: 1203 5647 6624 1203 Size: 4444 977 1327 Fragments arranged by size: 4444 1327 977 AgeI A'CCGG_T Cuts at: 5579 5579 Size: 6748 AhdI GACnn_n'nnGTC Cuts at: 5477 5477 Size: 6748 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6748 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6748 AlwNI CAG_nnn'CTG Cuts at: 2734 2839 2992 3079 3388 3919 4491 6063 2734 Size: 105 153 87 309 531 572 1572 3419 Fragments arranged by size: 3419 1572 572 531 309 153 105 87 ApaI G_GGCC'C Cuts at: 2600 3739 3793 2600 Size: 1139 54 5555 Fragments arranged by size: 5555 1139 54 ApaLI G'TGCA_C Cuts at: 2976 4805 5961 2976 Size: 1829 1156 3763 Fragments arranged by size: 3763 1829 1156 ApoI r'AATT_y Cuts at: 1493 2533 2551 4057 4283 4435 6544 1493 Size: 1040 18 1506 226 152 2109 1697 Fragments arranged by size: 2109 1697 1506 1040 226 152 18 AseI AT'TA_AT Cuts at: 160 1032 5302 160 Size: 872 4270 1606 Fragments arranged by size: 4270 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6748 AvaI C'yCGr_G Cuts at: 1870 1943 2235 3792 4016 4073 1870 Size: 73 292 1557 224 57 4545 Fragments arranged by size: 4545 1557 292 224 73 57 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2408 2441 2408 Size: 33 6715 BamHI G'GATC_C Cuts at: 4078 4445 4078 Size: 367 6381 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2189 2737 3806 4069 5524 Size: 325 217 627 402 548 1069 263 1455 Cuts at: 5524 618 Size: 1842 Fragments arranged by size: 1842 1455 1069 627 548 402 325 263 217 BanII G_rGCy'C Cuts at: 1391 2436 2600 2918 3739 3793 4067 1391 Size: 1045 164 318 821 54 274 4072 Fragments arranged by size: 4072 1045 821 318 274 164 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5777 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3542 3576 4939 4973 1903 Size: 34 1605 34 1363 34 3678 Fragments arranged by size: 3678 1605 1363 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4640 5850 4640 Size: 1210 5538 BclI T'GATC_A Cuts at: 1548 1548 Size: 6748 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2238 2604 2613 Size: 107 122 71 631 605 566 366 9 Cuts at: 2613 2913 3668 3773 5359 136 Size: 300 755 105 1586 1525 Fragments arranged by size: 1586 1525 755 631 605 566 366 300 122 107 105 71 9 BglII A'GATC_T Cuts at: 3366 6743 3366 Size: 3377 3371 BlpI GC'TnA_GC Cuts at: 3497 3780 3884 4165 3497 Size: 283 104 281 6080 Fragments arranged by size: 6080 283 281 104 Bme1580I G_kGCm'C Cuts at: 1790 2561 2600 2980 3169 3739 3793 4809 5965 Size: 771 39 380 189 570 54 1016 1156 Cuts at: 5965 1790 Size: 2573 Fragments arranged by size: 2573 1156 1016 771 570 380 189 54 39 BmgBI CAC'GTC Cuts at: 1318 3164 1318 Size: 1846 4902 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2967 4479 5437 Size: 416 53 83 399 24 1550 1512 958 Cuts at: 5437 442 Size: 1753 Fragments arranged by size: 1753 1550 1512 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6748 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2628 2701 2851 3014 3068 3101 3293 Size: 815 574 73 150 163 54 33 192 Cuts at: 3293 3557 3860 3941 5408 1239 Size: 264 303 81 1467 2579 Fragments arranged by size: 2579 1467 815 574 303 264 192 163 150 81 73 54 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3557 4873 5738 6036 3557 Size: 1316 865 298 4269 Fragments arranged by size: 4269 1316 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2303 5411 882 Size: 617 804 3108 2219 Fragments arranged by size: 3108 2219 804 617 BsaAI yAC'GTr Cuts at: 493 1204 3892 4045 4539 493 Size: 711 2688 153 494 2702 Fragments arranged by size: 2702 2688 711 494 153 BsaBI GATnn'nnATC Cuts at: 6742 6742 Size: 6748 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4555 4937 5572 275 Size: 53 83 186 936 3022 382 635 1451 Fragments arranged by size: 3022 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5181 5579 5853 6000 6411 5181 Size: 398 274 147 411 5518 Fragments arranged by size: 5518 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2436 2466 2626 2647 2656 Size: 30 165 30 1033 30 160 21 9 Cuts at: 2656 2677 2990 3020 3955 3985 1178 Size: 21 313 30 935 30 3941 Fragments arranged by size: 3941 1033 935 313 165 160 30 30 30 30 30 21 21 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3011 3098 3251 3500 3836 3857 4001 1186 Size: 1825 87 153 249 336 21 144 3933 Fragments arranged by size: 3933 1825 336 249 153 144 87 21 BseYI C'CCAG_C Cuts at: 2435 3168 3177 3541 3975 5951 6447 2435 Size: 733 9 364 434 1976 496 2736 Fragments arranged by size: 2736 1976 733 496 434 364 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6175 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2504 3603 4198 4959 5987 1540 Size: 275 186 503 1099 595 761 1028 2301 Fragments arranged by size: 2301 1099 1028 761 595 503 275 186 BsiHKAI G_wGCw'C Cuts at: 2486 2980 4809 4894 5965 2486 Size: 494 1829 85 1071 3269 Fragments arranged by size: 3269 1829 1071 494 85 BsmI GAATG_Cn' Cuts at: 4259 4352 4259 Size: 93 6655 BsmBI CGTCTCn'nnnn_ Cuts at: 2030 2030 Size: 6748 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2436 2486 2561 2600 2918 2980 3169 Size: 399 646 50 75 39 318 62 189 Cuts at: 3169 3739 3793 4067 4809 4894 5965 1391 Size: 570 54 274 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 646 570 399 318 274 189 85 75 62 54 50 39 BspEI T'CCGG_A Cuts at: 6411 6411 Size: 6748 BspHI T'CATG_A Cuts at: 4637 6367 4637 Size: 1730 5018 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3142 3187 3635 4103 844 Size: 2298 45 448 468 3489 Fragments arranged by size: 3489 2298 468 448 45 BsrBI CCG'CTC Cuts at: 1394 1823 3001 3088 3106 3726 3784 4635 1394 Size: 429 1178 87 18 620 58 851 3507 Fragments arranged by size: 3507 1178 851 620 429 87 58 18 BsrDI GCAATG_nn' Cuts at: 65 1248 5243 5417 65 Size: 1183 3995 174 1396 Fragments arranged by size: 3995 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3577 5392 5579 1700 Size: 110 1767 1815 187 2869 Fragments arranged by size: 2869 1815 1767 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6748 BssHII G'CGCG_C Cuts at: 1925 2050 3172 1925 Size: 125 1122 5501 Fragments arranged by size: 5501 1122 125 BssSI C'ACGA_G Cuts at: 4808 5820 4808 Size: 1012 5736 BstAPI GCAn_nnn'nTGC Cuts at: 794 3202 3956 794 Size: 2408 754 3586 Fragments arranged by size: 3586 2408 754 BstBI TT'CG_AA Cuts at: 2007 5552 2007 Size: 3545 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6748 BstXI CCAn_nnnn'nTGG Cuts at: 2455 3359 2455 Size: 904 5844 BstYI r'GATC_y Cuts at: 1972 3366 4078 4445 4830 4847 6299 6385 6397 Size: 1394 712 367 385 17 1452 86 12 Cuts at: 6397 6743 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1394 712 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2583 2583 Size: 6748 BtgI C'CryG_G Cuts at: 513 1065 2022 2090 2130 3591 3693 513 Size: 552 957 68 40 1461 102 3568 Fragments arranged by size: 3568 1461 957 552 102 68 40 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6009 Fragments arranged by size: 6009 683 56 BtsI GCAGTG_nn' Cuts at: 2152 2248 2254 2344 2401 2410 2778 4264 5058 Size: 96 6 90 57 9 368 1486 794 Cuts at: 5058 5078 2152 Size: 20 3822 Fragments arranged by size: 3822 1486 794 368 96 90 57 20 9 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6748 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6713 DraI TTT'AAA Cuts at: 4052 4052 Size: 6748 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5755 809 Size: 507 4439 1802 Fragments arranged by size: 4439 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2457 2501 Size: 54 1425 50 275 18 297 330 44 Cuts at: 2501 2900 3114 3285 3600 3621 3669 4195 5084 Size: 399 214 171 315 21 48 526 889 Cuts at: 5084 6713 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 526 399 330 315 297 275 214 171 54 50 48 44 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2501 3600 4195 1537 Size: 275 689 1099 595 4090 Fragments arranged by size: 4090 1099 689 595 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2174 3261 4678 1353 Size: 821 1087 1417 3423 Fragments arranged by size: 3423 1417 1087 821 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2631 2943 3419 3722 5335 5709 Size: 1064 138 1042 312 476 303 1613 374 Cuts at: 5709 5855 387 Size: 146 1280 Fragments arranged by size: 1613 1280 1064 1042 476 374 312 303 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2628 2701 2851 3014 3068 3101 Size: 393 422 574 73 150 163 54 33 Cuts at: 3101 3293 3398 3410 3499 3557 3860 3941 4811 Size: 192 105 12 89 58 303 81 870 Cuts at: 4811 5408 6195 6463 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 870 787 597 574 422 393 303 268 192 163 150 105 89 81 73 58 54 33 12 EcoO109I rG'GnC_Cy Cuts at: 2068 2596 2597 2836 2844 3184 3736 2068 Size: 528 1 239 8 340 552 5080 Fragments arranged by size: 5080 552 528 340 239 8 1 EcoRI G'AATT_C Cuts at: 4057 4435 4057 Size: 378 6370 FspI TGC'GCA Cuts at: 5254 5254 Size: 6748 HaeII r_GCGC'y Cuts at: 1678 3584 3802 3829 5895 1678 Size: 1906 218 27 2066 2531 Fragments arranged by size: 2531 2066 1906 218 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2468 2500 2626 2658 3640 Size: 32 135 32 896 32 126 32 982 Cuts at: 3640 3672 5389 5421 5463 5495 1373 Size: 32 1717 32 42 32 2626 Fragments arranged by size: 2626 1717 982 896 135 126 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4092 4338 669 Size: 988 281 2154 246 3079 Fragments arranged by size: 3079 2154 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6748 HpaI GTT'AAC Cuts at: 4338 4338 Size: 6748 KpnI G_GTAC'C Cuts at: 2193 4073 2193 Size: 1880 4868 MfeI C'AATT_G Cuts at: 4347 4347 Size: 6748 MluI A'CGCG_T Cuts at: 6624 6624 Size: 6748 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5862 6046 1305 Size: 4557 184 2007 Fragments arranged by size: 4557 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2129 2459 3623 10 Size: 54 1425 343 297 330 1164 3135 Fragments arranged by size: 3135 1425 1164 343 330 297 54 MslI CAynn'nnrTG Cuts at: 518 1166 2349 4706 5065 5224 518 Size: 648 1183 2357 359 159 2042 Fragments arranged by size: 2357 2042 1183 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2379 2648 2814 3138 3193 3229 3262 3388 Size: 773 269 166 324 55 36 33 126 Cuts at: 3388 3451 3493 3674 3877 3883 3919 4164 4841 Size: 63 42 181 203 6 36 245 677 Cuts at: 4841 5989 6234 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 773 677 324 269 245 245 203 181 166 126 63 55 42 36 36 33 6 NaeI GCC'GGC Cuts at: 3579 3579 Size: 6748 NcoI C'CATG_G Cuts at: 513 1065 2022 2130 513 Size: 552 957 108 5131 Fragments arranged by size: 5131 957 552 108 NdeI CA'TA_TG Cuts at: 387 387 Size: 6748 NgoMIV G'CCGG_C Cuts at: 3577 3577 Size: 6748 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6748 NotI GC'GGCC_GC Cuts at: 4195 4195 Size: 6748 NspI r_CATG'y Cuts at: 1132 1229 4106 5651 1132 Size: 97 2877 1545 2229 Fragments arranged by size: 2877 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2060 1348 Size: 712 6036 PciI A'CATG_T Cuts at: 5647 5647 Size: 6748 PflMI CCAn_nnn'nTGG Cuts at: 1108 2651 2734 2839 2992 3079 3919 1108 Size: 1543 83 105 153 87 840 3937 Fragments arranged by size: 3937 1543 840 153 105 87 83 PfoI T'CCnGG_A Cuts at: 2985 2985 Size: 6748 PmeI GTTT'AAAC Cuts at: 4052 4052 Size: 6748 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4817 4849 6330 6362 4817 Size: 32 1481 32 5203 Fragments arranged by size: 5203 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2068 2836 2844 2068 Size: 768 8 5972 Fragments arranged by size: 5972 768 8 PshAI GACnn'nnGTC Cuts at: 1613 2295 1613 Size: 682 6066 PsiI TTA'TAA Cuts at: 4318 4318 Size: 6748 PspOMI G'GGCC_C Cuts at: 2596 3735 3789 2596 Size: 1139 54 5555 Fragments arranged by size: 5555 1139 54 PspXI vC'TCGA_Gb Cuts at: 4016 4016 Size: 6748 PstI C_TGCA'G Cuts at: 830 3137 3198 3213 3234 3765 3882 4100 830 Size: 2307 61 15 21 531 117 218 3478 Fragments arranged by size: 3478 2307 531 218 117 61 21 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6748 PvuII CAG'CTG Cuts at: 3193 3229 3388 3451 3493 3877 3883 3919 3193 Size: 36 159 63 42 384 6 36 6022 Fragments arranged by size: 6022 384 159 63 42 36 36 6 SalI G'TCGA_C Cuts at: 1936 4090 1936 Size: 2154 4594 SanDI GG'GwC_CC Cuts at: 2068 2068 Size: 6748 SapI GCTCTTCn'nnn_ Cuts at: 2174 3261 2174 Size: 1087 5661 SbfI CC_TGCA'GG Cuts at: 4100 4100 Size: 6748 ScaI AGT'ACT Cuts at: 4996 6708 4996 Size: 1712 5036 SfcI C'TryA_G Cuts at: 826 1045 2710 2722 3133 3146 3188 3194 3209 Size: 219 1665 12 411 13 42 6 15 Cuts at: 3209 3230 3761 3878 4096 5231 5912 6103 826 Size: 21 531 117 218 1135 681 191 1471 Fragments arranged by size: 1665 1471 1135 681 531 411 219 218 191 117 42 21 15 13 12 6 SfiI GGCCn_nnn'nGGCC Cuts at: 2604 3668 2604 Size: 1064 5684 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6748 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6748 SmaI CCC'GGG Cuts at: 1872 3794 4075 1872 Size: 1922 281 4545 Fragments arranged by size: 4545 1922 281 SmlI C'TyrA_G Cuts at: 820 1017 1943 3572 4016 4852 5753 6015 820 Size: 197 926 1629 444 836 901 262 1553 Fragments arranged by size: 1629 1553 926 901 836 444 262 197 SnaBI TAC'GTA Cuts at: 493 4045 4539 493 Size: 3552 494 2702 Fragments arranged by size: 3552 2702 494 SpeI A'CTAG_T Cuts at: 152 2494 152 Size: 2342 4406 SphI G_CATG'C Cuts at: 1132 4106 1132 Size: 2974 3774 SspI AAT'ATT Cuts at: 5 52 4672 5 Size: 47 4620 2081 Fragments arranged by size: 4620 2081 47 StuI AGG'CCT Cuts at: 1517 2940 3998 1517 Size: 1423 1058 4267 Fragments arranged by size: 4267 1423 1058 StyI C'CwwG_G Cuts at: 513 1065 2022 2130 3684 4187 513 Size: 552 957 108 1554 503 3074 Fragments arranged by size: 3074 1554 957 552 503 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2457 2461 4775 4792 4945 5130 5575 Size: 1255 877 4 2314 17 153 185 445 Cuts at: 5575 325 Size: 1498 Fragments arranged by size: 2314 1498 1255 877 445 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4994 6706 96 Size: 274 80 33 51 1443 3017 1712 138 Fragments arranged by size: 3017 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2436 2468 2647 2679 2990 3022 3323 3355 3955 Size: 32 179 32 311 32 301 32 600 Cuts at: 3955 3987 6708 6740 2436 Size: 32 2721 32 2444 Fragments arranged by size: 2721 2444 600 311 301 179 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6748 XbaI T'CTAG_A Cuts at: 4084 4084 Size: 6748 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2137 2182 2915 3538 2029 Size: 108 45 733 623 5239 Fragments arranged by size: 5239 733 623 108 45 XhoI C'TCGA_G Cuts at: 1943 4016 1943 Size: 2073 4675 XmaI C'CCGG_G Cuts at: 1870 3792 4073 1870 Size: 1922 281 4545 Fragments arranged by size: 4545 1922 281 XmnI GAAnn'nnTTC Cuts at: 4877 4877 Size: 6748 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4556 276 Size: 53 83 186 936 3022 2468 Fragments arranged by size: 3022 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacI SacII SexAI SfoI SrfI SwaI