Restriction map for
pFN21ASDA0552
(Linear) (Six-base) MAPSORT of: KIAA0552.seq Check: 4352 from: 1 to: 2019
KIAA0552.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1119 1164 1612 2019
Size: 1119 45 448 407
Fragments arranged by size:
1119 448 407 45
Acc65I G'GTAC_C
Cuts at: 0 166 2019
Size: 166 1853
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1375 1387 1476 2019
Size: 1375 12 89 543
Fragments arranged by size:
1375 543 89 12
AlwNI CAG_nnn'CTG
Cuts at: 0 711 816 969 1056 1365 1896 2019
Size: 711 105 153 87 309 531 123
Fragments arranged by size:
711 531 309 153 123 105 87
ApaI G_GGCC'C
Cuts at: 0 577 1716 1770 2019
Size: 577 1139 54 249
Fragments arranged by size:
1139 577 249 54
ApaLI G'TGCA_C
Cuts at: 0 953 2019
Size: 953 1066
ApoI r'AATT_y
Cuts at: 0 510 528 2019
Size: 510 18 1491
Fragments arranged by size:
1491 510 18
AvaI C'yCGr_G
Cuts at: 0 212 1769 1993 2019
Size: 212 1557 224 26
Fragments arranged by size:
1557 224 212 26
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 385 418 2019
Size: 385 33 1601
Fragments arranged by size:
1601 385 33
BanI G'GyrC_C
Cuts at: 0 166 714 1783 2019
Size: 166 548 1069 236
Fragments arranged by size:
1069 548 236 166
BanII G_rGCy'C
Cuts at: 0 413 577 895 1716 1770 2019
Size: 413 164 318 821 54 249
Fragments arranged by size:
821 413 318 249 164 54
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1519 1553 2019
Size: 1519 34 466
Fragments arranged by size:
1519 466 34
BglI GCCn_nnn'nGGC
Cuts at: 0 215 581 590 890 1645 1750 2019
Size: 215 366 9 300 755 105 269
Fragments arranged by size:
755 366 300 269 215 105 9
BglII A'GATC_T
Cuts at: 0 1343 2019
Size: 1343 676
BlpI GC'TnA_GC
Cuts at: 0 1474 1757 1861 2019
Size: 1474 283 104 158
Fragments arranged by size:
1474 283 158 104
Bme1580I G_kGCm'C
Cuts at: 0 538 577 957 1146 1716 1770 2019
Size: 538 39 380 189 570 54 249
Fragments arranged by size:
570 538 380 249 189 54 39
BmgBI CAC'GTC
Cuts at: 0 1141 2019
Size: 1141 878
BmrI ACTGGGnnnn_n'
Cuts at: 0 944 2019
Size: 944 1075
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 605 678 828 991 1045 1078 1270
Size: 31 574 73 150 163 54 33 192
Cuts at: 1270 1534 1837 1918 2019
Size: 264 303 81 101
Fragments arranged by size:
574 303 264 192 163 150 101 81
73 54 33 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1534 2019
Size: 1534 485
BsaI GGTCTCn'nnnn_
Cuts at: 0 280 2019
Size: 280 1739
BsaAI yAC'GTr
Cuts at: 0 1869 2019
Size: 1869 150
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 413 443 603 624 633 654 967 997
Size: 413 30 160 21 9 21 313 30
Cuts at: 997 1932 1962 2019
Size: 935 30 57
Fragments arranged by size:
935 413 313 160 57 30 30 30
21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 988 1075 1228 1477 1813 1834 1978 2019
Size: 988 87 153 249 336 21 144 41
Fragments arranged by size:
988 336 249 153 144 87 41 21
BseYI C'CCAG_C
Cuts at: 0 412 1145 1154 1518 1952 2019
Size: 412 733 9 364 434 67
Fragments arranged by size:
733 434 412 364 67 9
BsiEI CG_ry'CG
Cuts at: 0 481 1580 2019
Size: 481 1099 439
Fragments arranged by size:
1099 481 439
BsiHKAI G_wGCw'C
Cuts at: 0 463 957 2019
Size: 463 494 1062
Fragments arranged by size:
1062 494 463
BsmBI CGTCTCn'nnnn_
Cuts at: 0 7 2019
Size: 7 2012
Bsp1286I G_dGCh'C
Cuts at: 0 413 463 538 577 895 957 1146 1716
Size: 413 50 75 39 318 62 189 570
Cuts at: 1716 1770 2019
Size: 54 249
Fragments arranged by size:
570 413 318 249 189 75 62 54
50 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1119 1164 1612 2019
Size: 1119 45 448 407
Fragments arranged by size:
1119 448 407 45
BsrBI CCG'CTC
Cuts at: 0 978 1065 1083 1703 1761 2019
Size: 978 87 18 620 58 258
Fragments arranged by size:
978 620 258 87 58 18
BsrFI r'CCGG_y
Cuts at: 0 1554 2019
Size: 1554 465
BssHII G'CGCG_C
Cuts at: 0 27 1149 2019
Size: 27 1122 870
Fragments arranged by size:
1122 870 27
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1179 1933 2019
Size: 1179 754 86
Fragments arranged by size:
1179 754 86
BstXI CCAn_nnnn'nTGG
Cuts at: 0 432 1336 2019
Size: 432 904 683
Fragments arranged by size:
904 683 432
BstYI r'GATC_y
Cuts at: 0 1343 2019
Size: 1343 676
Bsu36I CC'TnA_GG
Cuts at: 0 560 2019
Size: 560 1459
BtgI C'CryG_G
Cuts at: 0 67 107 1568 1670 2019
Size: 67 40 1461 102 349
Fragments arranged by size:
1461 349 102 67 40
BtsI GCAGTG_nn'
Cuts at: 0 129 225 231 321 378 387 755 2019
Size: 129 96 6 90 57 9 368 1264
Fragments arranged by size:
1264 368 129 96 90 57 9 6
EaeI y'GGCC_r
Cuts at: 0 104 434 478 877 1091 1262 1577 1598
Size: 104 330 44 399 214 171 315 21
Cuts at: 1598 1646 2019
Size: 48 373
Fragments arranged by size:
399 373 330 315 214 171 104 48
44 21
EagI C'GGCC_G
Cuts at: 0 478 1577 2019
Size: 478 1099 442
Fragments arranged by size:
1099 478 442
EarI CTCTTCn'nnn_
Cuts at: 0 151 1238 2019
Size: 151 1087 781
Fragments arranged by size:
1087 781 151
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 608 920 1396 1699 2019
Size: 608 312 476 303 320
Fragments arranged by size:
608 476 320 312 303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 605 678 828 991 1045 1078 1270
Size: 31 574 73 150 163 54 33 192
Cuts at: 1270 1375 1387 1476 1534 1837 1918 2019
Size: 105 12 89 58 303 81 101
Fragments arranged by size:
574 303 192 163 150 105 101 89
81 73 58 54 33 31 12
EcoO109I rG'GnC_Cy
Cuts at: 0 45 573 574 813 821 1161 1713 2019
Size: 45 528 1 239 8 340 552 306
Fragments arranged by size:
552 528 340 306 239 45 8 1
HaeII r_GCGC'y
Cuts at: 0 1561 1779 1806 2019
Size: 1561 218 27 213
Fragments arranged by size:
1561 218 213 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 445 477 603 635 1617 1649 2019
Size: 445 32 126 32 982 32 370
Fragments arranged by size:
982 445 370 126 32 32 32
KpnI G_GTAC'C
Cuts at: 0 170 2019
Size: 170 1849
MscI TGG'CCA
Cuts at: 0 106 436 1600 2019
Size: 106 330 1164 419
Fragments arranged by size:
1164 419 330 106
MslI CAynn'nnrTG
Cuts at: 0 326 2019
Size: 326 1693
MspA1I CmG'CkG
Cuts at: 0 356 625 791 1115 1170 1206 1239 1365
Size: 356 269 166 324 55 36 33 126
Cuts at: 1365 1428 1470 1651 1854 1860 1896 2019
Size: 63 42 181 203 6 36 123
Fragments arranged by size:
356 324 269 203 181 166 126 123
63 55 42 36 36 33 6
NaeI GCC'GGC
Cuts at: 0 1556 2019
Size: 1556 463
NcoI C'CATG_G
Cuts at: 0 107 2019
Size: 107 1912
NgoMIV G'CCGG_C
Cuts at: 0 1554 2019
Size: 1554 465
PasI CC'CwG_GG
Cuts at: 0 37 2019
Size: 37 1982
PflMI CCAn_nnn'nTGG
Cuts at: 0 628 711 816 969 1056 1896 2019
Size: 628 83 105 153 87 840 123
Fragments arranged by size:
840 628 153 123 105 87 83
PfoI T'CCnGG_A
Cuts at: 0 962 2019
Size: 962 1057
PpuMI rG'GwC_Cy
Cuts at: 0 45 813 821 2019
Size: 45 768 8 1198
Fragments arranged by size:
1198 768 45 8
PshAI GACnn'nnGTC
Cuts at: 0 272 2019
Size: 272 1747
PspOMI G'GGCC_C
Cuts at: 0 573 1712 1766 2019
Size: 573 1139 54 253
Fragments arranged by size:
1139 573 253 54
PspXI vC'TCGA_Gb
Cuts at: 0 1993 2019
Size: 1993 26
PstI C_TGCA'G
Cuts at: 0 1114 1175 1190 1211 1742 1859 2019
Size: 1114 61 15 21 531 117 160
Fragments arranged by size:
1114 531 160 117 61 21 15
PvuII CAG'CTG
Cuts at: 0 1170 1206 1365 1428 1470 1854 1860 1896
Size: 1170 36 159 63 42 384 6 36
Cuts at: 1896 2019
Size: 123
Fragments arranged by size:
1170 384 159 123 63 42 36 36
6
SanDI GG'GwC_CC
Cuts at: 0 45 2019
Size: 45 1974
SapI GCTCTTCn'nnn_
Cuts at: 0 151 1238 2019
Size: 151 1087 781
Fragments arranged by size:
1087 781 151
SfcI C'TryA_G
Cuts at: 0 687 699 1110 1123 1165 1171 1186 1207
Size: 687 12 411 13 42 6 15 21
Cuts at: 1207 1738 1855 2019
Size: 531 117 164
Fragments arranged by size:
687 531 411 164 117 42 21 15
13 12 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 581 1645 2019
Size: 581 1064 374
Fragments arranged by size:
1064 581 374
SmaI CCC'GGG
Cuts at: 0 1771 2019
Size: 1771 248
SmlI C'TyrA_G
Cuts at: 0 1549 1993 2019
Size: 1549 444 26
Fragments arranged by size:
1549 444 26
SpeI A'CTAG_T
Cuts at: 0 471 2019
Size: 471 1548
StuI AGG'CCT
Cuts at: 0 917 1975 2019
Size: 917 1058 44
Fragments arranged by size:
1058 917 44
StyI C'CwwG_G
Cuts at: 0 107 1661 2019
Size: 107 1554 358
Fragments arranged by size:
1554 358 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 434 438 2019
Size: 434 4 1581
Fragments arranged by size:
1581 434 4
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 413 445 624 656 967 999 1300 1332
Size: 413 32 179 32 311 32 301 32
Cuts at: 1332 1932 1964 2019
Size: 600 32 55
Fragments arranged by size:
600 413 311 301 179 55 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 114 159 892 1515 2019
Size: 114 45 733 623 504
Fragments arranged by size:
733 623 504 114 45
XhoI C'TCGA_G
Cuts at: 0 1993 2019
Size: 1993 26
XmaI C'CCGG_G
Cuts at: 0 1769 2019
Size: 1769 250
Enzymes that do cut:
AarI Acc65I AcuI AlwNI ApaI ApaLI ApoI AvaI
BaeI BanI BanII BcgI BglI BglII BlpI Bme1580I
BmgBI BmrI BpmI BpuEI BsaI BsaAI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI
BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI
EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I KpnI
MscI MslI MspA1I NaeI NcoI NgoMIV PasI PflMI
PfoI PpuMI PshAI PspOMI PspXI PstI PvuII SanDI
SapI SfcI SfiI SmaI SmlI SpeI StuI StyI
TaqII TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AscI AseI AsiSI AvrII BamHI
BbeI BbsI BbvCI BciVI BclI BfrBI BmtI BplI
Bpu10I BsaBI BsaHI BsaWI BsgI BsiWI BsmI BspEI
BspHI BsrDI BsrGI BssSI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI MfeI MluI MmeI NarI NdeI NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PsiI PsrI PvuI RsrII SacI SacII SalI SbfI
ScaI SexAI SfoI SgfI SgrAI SnaBI SphI SrfI
SspI SwaI TatI Tth111I XbaI XmnI ZraI