(Circular) (Six-base) MAPSORT of: pFN21ASDA0535.seq Check: 3542 from: 1 to: 7870 pFN21ASDA0535 7870 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5680 278 Size: 53 83 186 936 4144 2468 Fragments arranged by size: 4144 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5213 1937 Size: 3276 4594 Acc65I G'GTAC_C Cuts at: 5191 5191 Size: 7870 AclI AA'CG_TT Cuts at: 1563 5997 6370 1563 Size: 4434 373 3063 Fragments arranged by size: 4434 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2058 2169 2757 4761 4856 5933 7317 7585 Size: 426 111 588 2004 95 1077 1384 268 Cuts at: 7585 1632 Size: 1917 Fragments arranged by size: 2004 1917 1384 1077 588 426 268 111 95 AflII C'TTAA_G Cuts at: 820 1017 3868 4319 820 Size: 197 2851 451 4371 Fragments arranged by size: 4371 2851 451 197 AflIII A'CryG_T Cuts at: 1203 3154 6769 7746 1203 Size: 1951 3615 977 1327 Fragments arranged by size: 3615 1951 1327 977 AgeI A'CCGG_T Cuts at: 6701 6701 Size: 7870 AhdI GACnn_n'nnGTC Cuts at: 6599 6599 Size: 7870 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7870 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2536 2967 1184 Size: 1352 431 6087 Fragments arranged by size: 6087 1352 431 AlwNI CAG_nnn'CTG Cuts at: 2036 3746 3749 3817 4535 5613 7185 2036 Size: 1710 3 68 718 1078 1572 2721 Fragments arranged by size: 2721 1710 1572 1078 718 68 3 ApaLI G'TGCA_C Cuts at: 5927 7083 5927 Size: 1156 6714 ApoI r'AATT_y Cuts at: 1493 2426 2464 2747 2926 3225 3446 3641 4099 Size: 933 38 283 179 299 221 195 458 Cuts at: 4099 5044 5179 5405 5557 7666 1493 Size: 945 135 226 152 2109 1697 Fragments arranged by size: 2109 1697 945 933 458 299 283 226 221 195 179 152 135 38 AseI AT'TA_AT Cuts at: 160 1032 6424 160 Size: 872 5392 1606 Fragments arranged by size: 5392 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7870 AvaI C'yCGr_G Cuts at: 1870 1943 4348 5195 1870 Size: 73 2405 847 4545 Fragments arranged by size: 4545 2405 847 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2368 2401 2368 Size: 33 7837 BamHI G'GATC_C Cuts at: 5200 5567 5200 Size: 367 7503 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2077 3195 3668 5191 6646 Size: 325 217 627 290 1118 473 1523 1455 Cuts at: 6646 618 Size: 1842 Fragments arranged by size: 1842 1523 1455 1118 627 473 325 290 217 BanII G_rGCy'C Cuts at: 1391 2106 2397 3168 3686 4091 5189 1391 Size: 715 291 771 518 405 1098 4072 Fragments arranged by size: 4072 1098 771 715 518 405 291 BbeI G_GCGC'C Cuts at: 3672 3672 Size: 7870 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2244 2324 2376 3076 3732 4061 4458 Size: 971 345 80 52 700 656 329 397 Cuts at: 4458 928 Size: 4340 Fragments arranged by size: 4340 971 700 656 397 345 329 80 52 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6061 6095 1903 Size: 34 4124 34 3678 Fragments arranged by size: 4124 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5165 5762 6972 5165 Size: 597 1210 6063 Fragments arranged by size: 6063 1210 597 BclI T'GATC_A Cuts at: 1548 1548 Size: 7870 BfrBI ATG'CAT Cuts at: 3238 4241 3238 Size: 1003 6867 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6481 136 Size: 107 122 71 631 605 4809 1525 Fragments arranged by size: 4809 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4168 7865 4168 Size: 3697 4173 BlpI GC'TnA_GC Cuts at: 2964 5287 2964 Size: 2323 5547 Bme1580I G_kGCm'C Cuts at: 1790 3128 3200 4356 5931 7087 1790 Size: 1338 72 1156 1575 1156 2573 Fragments arranged by size: 2573 1575 1338 1156 1156 72 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7870 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3405 4076 4782 Size: 416 53 83 399 24 1988 671 706 Cuts at: 4782 5601 6559 442 Size: 819 958 1753 Fragments arranged by size: 1988 1753 958 819 706 671 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7870 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4607 4607 Size: 7870 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2341 2960 3038 4143 6530 1239 Size: 1102 619 78 1105 2387 2579 Fragments arranged by size: 2579 2387 1105 1102 619 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3236 5995 6860 7158 3236 Size: 2759 865 298 3948 Fragments arranged by size: 3948 2759 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3110 6533 882 Size: 617 1611 3423 2219 Fragments arranged by size: 3423 2219 1611 617 BsaAI yAC'GTr Cuts at: 493 1204 3157 4225 4357 5167 5661 493 Size: 711 1953 1068 132 810 494 2702 Fragments arranged by size: 2702 1953 1068 810 711 494 132 BsaBI GATnn'nnATC Cuts at: 3372 7864 3372 Size: 4492 3378 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3669 5677 6059 6694 Size: 53 83 186 936 2136 2008 382 635 Cuts at: 6694 275 Size: 1451 Fragments arranged by size: 2136 2008 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4391 6303 6701 6975 7122 7533 4391 Size: 1912 398 274 147 411 4728 Fragments arranged by size: 4728 1912 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2624 2654 3107 3137 3782 Size: 30 165 30 1221 30 453 30 645 Cuts at: 3782 3812 4307 4337 1178 Size: 30 495 30 4711 Fragments arranged by size: 4711 1221 645 495 453 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2575 2873 4076 4079 4931 4934 1186 Size: 1389 298 1203 3 852 3 4122 Fragments arranged by size: 4122 1389 1203 852 298 3 3 BseYI C'CCAG_C Cuts at: 2628 2835 4017 4651 7073 7569 2628 Size: 207 1182 634 2422 496 2929 Fragments arranged by size: 2929 2422 1182 634 496 207 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2328 2637 3684 3888 1163 Size: 573 592 309 1047 204 5145 Fragments arranged by size: 5145 1047 592 573 309 204 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5320 6081 7109 1540 Size: 275 186 3319 761 1028 2301 Fragments arranged by size: 3319 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2106 2397 3024 3252 4091 5931 6016 7087 2106 Size: 291 627 228 839 1840 85 1071 2889 Fragments arranged by size: 2889 1840 1071 839 627 291 228 85 BsmI GAATG_Cn' Cuts at: 2809 3969 3986 5117 5381 5474 2809 Size: 1160 17 1131 264 93 5205 Fragments arranged by size: 5205 1160 1131 264 93 17 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2397 3024 3128 3168 3200 3252 Size: 399 316 291 627 104 40 32 52 Cuts at: 3252 3686 4091 4356 5189 5931 6016 7087 1391 Size: 434 405 265 833 742 85 1071 2174 Fragments arranged by size: 2174 1071 833 742 627 434 405 399 316 291 265 104 85 52 40 32 BspEI T'CCGG_A Cuts at: 4391 7533 4391 Size: 3142 4728 BspHI T'CATG_A Cuts at: 2307 4749 5759 7489 2307 Size: 2442 1010 1730 2688 Fragments arranged by size: 2688 2442 1730 1010 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4961 5225 844 Size: 4117 264 3489 Fragments arranged by size: 4117 3489 264 BsrBI CCG'CTC Cuts at: 1394 1823 5757 1394 Size: 429 3934 3507 Fragments arranged by size: 3934 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2836 3363 4651 6365 6539 65 Size: 1183 1588 527 1288 1714 174 1396 Fragments arranged by size: 1714 1588 1396 1288 1183 527 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6514 6701 1700 Size: 110 4704 187 2869 Fragments arranged by size: 4704 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 5079 96 Size: 4983 2887 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7870 BssSI C'ACGA_G Cuts at: 4401 5930 6942 4401 Size: 1529 1012 5329 Fragments arranged by size: 5329 1529 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3746 3749 794 Size: 2952 3 4915 Fragments arranged by size: 4915 2952 3 BstBI TT'CG_AA Cuts at: 2007 6674 2007 Size: 4667 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7870 BstXI CCAn_nnnn'nTGG Cuts at: 3686 3686 Size: 7870 BstYI r'GATC_y Cuts at: 1972 4168 5200 5567 5952 5969 7421 7507 7519 Size: 2196 1032 367 385 17 1452 86 12 Cuts at: 7519 7865 1972 Size: 346 1977 Fragments arranged by size: 2196 1977 1452 1032 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2127 513 Size: 552 957 105 6256 Fragments arranged by size: 6256 957 552 105 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2520 4232 4356 487 Size: 683 56 1294 1712 124 4001 Fragments arranged by size: 4001 1712 1294 683 124 56 BtsI GCAGTG_nn' Cuts at: 2251 3407 4258 5151 5386 6180 6200 2251 Size: 1156 851 893 235 794 20 3921 Fragments arranged by size: 3921 1156 893 851 794 235 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7870 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7835 DraI TTT'AAA Cuts at: 2469 2493 5174 2469 Size: 24 2681 5165 Fragments arranged by size: 5165 2681 24 DraIII CAC_nnn'GTG Cuts at: 3208 3208 Size: 7870 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6877 809 Size: 507 5561 1802 Fragments arranged by size: 5561 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3688 4219 4489 Size: 54 1425 50 275 18 1858 531 270 Cuts at: 4489 5317 6206 7835 8 Size: 828 889 1629 43 Fragments arranged by size: 1858 1629 1425 889 828 531 275 270 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5317 1537 Size: 275 3505 4090 Fragments arranged by size: 4090 3505 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2132 2542 2889 3952 5800 1353 Size: 779 410 347 1063 1848 3423 Fragments arranged by size: 3423 1848 1063 779 410 347 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6457 6831 6977 387 Size: 1064 138 4868 374 146 1280 Fragments arranged by size: 4868 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2104 2395 4089 2104 Size: 291 1694 5885 Fragments arranged by size: 5885 1694 291 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2058 2169 2341 2757 2960 3038 4143 Size: 393 426 111 172 416 203 78 1105 Cuts at: 4143 4761 4856 5933 6530 7317 7585 1239 Size: 618 95 1077 597 787 268 1524 Fragments arranged by size: 1524 1105 1077 787 618 597 426 416 393 268 203 172 111 95 78 EcoNI CCTnn'n_nnAGG Cuts at: 2912 3663 4476 2912 Size: 751 813 6306 Fragments arranged by size: 6306 813 751 EcoO109I rG'GnC_Cy Cuts at: 5027 5027 Size: 7870 EcoRI G'AATT_C Cuts at: 3446 5179 5557 3446 Size: 1733 378 5759 Fragments arranged by size: 5759 1733 378 FspI TGC'GCA Cuts at: 6376 6376 Size: 7870 HaeII r_GCGC'y Cuts at: 1678 3672 7017 1678 Size: 1994 3345 2531 Fragments arranged by size: 3345 2531 1994 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2363 2395 2624 2656 3782 Size: 32 135 32 791 32 229 32 1126 Cuts at: 3782 3814 4689 4721 6511 6543 6585 6617 1373 Size: 32 875 32 1790 32 42 32 2626 Fragments arranged by size: 2626 1790 1126 875 791 229 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2176 5214 5460 669 Size: 988 281 238 3038 246 3079 Fragments arranged by size: 3079 3038 988 281 246 238 HindIII A'AGCT_T Cuts at: 748 4154 4384 4687 748 Size: 3406 230 303 3931 Fragments arranged by size: 3931 3406 303 230 HpaI GTT'AAC Cuts at: 5460 5460 Size: 7870 KasI G'GCGC_C Cuts at: 3668 3668 Size: 7870 KpnI G_GTAC'C Cuts at: 5195 5195 Size: 7870 MfeI C'AATT_G Cuts at: 2949 3333 5469 2949 Size: 384 2136 5350 Fragments arranged by size: 5350 2136 384 MluI A'CGCG_T Cuts at: 7746 7746 Size: 7870 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2667 3825 3959 4220 4352 4863 6984 7168 Size: 1362 1158 134 261 132 511 2121 184 Cuts at: 7168 1305 Size: 2007 Fragments arranged by size: 2121 2007 1362 1158 511 261 184 134 132 MscI TGG'CCA Cuts at: 10 64 1489 1832 4221 4491 10 Size: 54 1425 343 2389 270 3389 Fragments arranged by size: 3389 2389 1425 343 270 54 MslI CAynn'nnrTG Cuts at: 518 1166 2538 3243 4204 4251 4809 4889 5075 Size: 648 1372 705 961 47 558 80 186 Cuts at: 5075 5828 6187 6346 518 Size: 753 359 159 2042 Fragments arranged by size: 2042 1372 961 753 705 648 558 359 186 159 80 47 MspA1I CmG'CkG Cuts at: 1606 2147 3344 3746 3774 5286 5963 7111 7356 Size: 541 1197 402 28 1512 677 1148 245 Cuts at: 7356 1606 Size: 2120 Fragments arranged by size: 2120 1512 1197 1148 677 541 402 245 28 NarI GG'CG_CC Cuts at: 3669 3669 Size: 7870 NcoI C'CATG_G Cuts at: 513 1065 2022 2127 513 Size: 552 957 105 6256 Fragments arranged by size: 6256 957 552 105 NdeI CA'TA_TG Cuts at: 387 5056 387 Size: 4669 3201 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7870 NotI GC'GGCC_GC Cuts at: 5317 5317 Size: 7870 NsiI A_TGCA'T Cuts at: 3240 4243 3240 Size: 1003 6867 NspI r_CATG'y Cuts at: 1132 1229 3824 5228 6773 1132 Size: 97 2595 1404 1545 2229 Fragments arranged by size: 2595 2229 1545 1404 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7870 PciI A'CATG_T Cuts at: 6769 6769 Size: 7870 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7870 PfoI T'CCnGG_A Cuts at: 2097 3891 4120 4595 2097 Size: 1794 229 475 5372 Fragments arranged by size: 5372 1794 475 229 PmeI GTTT'AAAC Cuts at: 5174 5174 Size: 7870 PmlI CAC'GTG Cuts at: 3157 4225 4357 3157 Size: 1068 132 6670 Fragments arranged by size: 6670 1068 132 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5939 5971 7452 7484 5939 Size: 32 1481 32 6325 Fragments arranged by size: 6325 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5027 5027 Size: 7870 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7870 PsiI TTA'TAA Cuts at: 5440 5440 Size: 7870 PstI C_TGCA'G Cuts at: 830 2961 3343 3779 5222 830 Size: 2131 382 436 1443 3478 Fragments arranged by size: 3478 2131 1443 436 382 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7870 PvuII CAG'CTG Cuts at: 2147 3344 3746 2147 Size: 1197 402 6271 Fragments arranged by size: 6271 1197 402 SacI G_AGCT'C Cuts at: 2106 2397 4091 2106 Size: 291 1694 5885 Fragments arranged by size: 5885 1694 291 SalI G'TCGA_C Cuts at: 1936 5212 1936 Size: 3276 4594 SbfI CC_TGCA'GG Cuts at: 5222 5222 Size: 7870 ScaI AGT'ACT Cuts at: 6118 7830 6118 Size: 1712 6158 SfcI C'TryA_G Cuts at: 826 1045 2212 2957 3339 3775 4036 5218 6353 Size: 219 1167 745 382 436 261 1182 1135 Cuts at: 6353 7034 7225 826 Size: 681 191 1471 Fragments arranged by size: 1471 1182 1167 1135 745 681 436 382 261 219 191 SfoI GGC'GCC Cuts at: 3670 3670 Size: 7870 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7870 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7870 SmaI CCC'GGG Cuts at: 1872 5197 1872 Size: 3325 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3251 3868 4319 5974 6875 7137 Size: 197 926 1308 617 451 1655 901 262 Cuts at: 7137 820 Size: 1553 Fragments arranged by size: 1655 1553 1308 926 901 617 451 262 197 SnaBI TAC'GTA Cuts at: 493 5167 5661 493 Size: 4674 494 2702 Fragments arranged by size: 4674 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7870 SphI G_CATG'C Cuts at: 1132 3824 5228 1132 Size: 2692 1404 3774 Fragments arranged by size: 3774 2692 1404 SspI AAT'ATT Cuts at: 5 52 5794 5 Size: 47 5742 2081 Fragments arranged by size: 5742 2081 47 StuI AGG'CCT Cuts at: 1517 3103 4130 1517 Size: 1586 1027 5257 Fragments arranged by size: 5257 1586 1027 StyI C'CwwG_G Cuts at: 513 1065 2022 2127 2271 2919 3098 3825 4437 Size: 552 957 105 144 648 179 727 612 Cuts at: 4437 4492 5309 513 Size: 55 817 3074 Fragments arranged by size: 3074 957 817 727 648 612 552 179 144 105 55 SwaI ATTT'AAAT Cuts at: 2469 2469 Size: 7870 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3053 3323 3692 3746 4694 5897 5914 Size: 1255 1473 270 369 54 948 1203 17 Cuts at: 5914 6067 6252 6697 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1473 1255 1203 948 445 369 270 185 153 54 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5079 6116 7828 Size: 274 80 33 51 1443 3102 1037 1712 Cuts at: 7828 96 Size: 138 Fragments arranged by size: 3102 1712 1443 1037 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2061 2093 2658 2690 3107 3139 3916 3948 4307 Size: 32 565 32 417 32 777 32 359 Cuts at: 4307 4339 4402 4434 7830 7862 2061 Size: 32 63 32 3396 32 2069 Fragments arranged by size: 3396 2069 777 565 417 359 63 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7870 XbaI T'CTAG_A Cuts at: 4093 5206 4093 Size: 1113 6757 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7870 XmaI C'CCGG_G Cuts at: 1870 5195 1870 Size: 3325 4545 XmnI GAAnn'nnTTC Cuts at: 2331 5999 2331 Size: 3668 4202 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5678 276 Size: 53 83 186 936 4144 2468 Fragments arranged by size: 4144 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI XcmI