(Circular) (Six-base) MAPSORT of: pFN21ASDA0526.seq Check: 238 from: 1 to: 6415 pFN21ASDA0526 6415 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4225 278 Size: 53 83 186 936 2689 2468 Fragments arranged by size: 2689 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2507 3758 1937 Size: 570 1251 4594 Fragments arranged by size: 4594 1251 570 Acc65I G'GTAC_C Cuts at: 3655 3736 3655 Size: 81 6334 AclI AA'CG_TT Cuts at: 1563 3149 4542 4915 1563 Size: 1586 1393 373 3063 Fragments arranged by size: 3063 1586 1393 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3027 3503 3653 4478 5862 6130 1632 Size: 1395 476 150 825 1384 268 1917 Fragments arranged by size: 1917 1395 1384 825 476 268 150 AfeI AGC'GCT Cuts at: 2122 2122 Size: 6415 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6218 AflIII A'CryG_T Cuts at: 1203 5314 6291 1203 Size: 4111 977 1327 Fragments arranged by size: 4111 1327 977 AgeI A'CCGG_T Cuts at: 5246 5246 Size: 6415 AhdI GACnn_n'nnGTC Cuts at: 2356 5144 2356 Size: 2788 3627 AleI CACnn'nnGTG Cuts at: 1166 2246 1166 Size: 1080 5335 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6415 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2979 3011 2979 Size: 32 6383 AlwNI CAG_nnn'CTG Cuts at: 4158 5730 4158 Size: 1572 4843 ApaI G_GGCC'C Cuts at: 3083 3083 Size: 6415 ApaLI G'TGCA_C Cuts at: 4472 5628 4472 Size: 1156 5259 ApoI r'AATT_y Cuts at: 1493 2746 2949 3724 3950 4102 6211 1493 Size: 1253 203 775 226 152 2109 1697 Fragments arranged by size: 2109 1697 1253 775 226 203 152 AseI AT'TA_AT Cuts at: 160 1032 4969 160 Size: 872 3937 1606 Fragments arranged by size: 3937 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6415 AvaI C'yCGr_G Cuts at: 1870 1943 3122 3740 1870 Size: 73 1179 618 4545 Fragments arranged by size: 4545 1179 618 73 BamHI G'GATC_C Cuts at: 3118 3745 4112 3118 Size: 627 367 5421 Fragments arranged by size: 5421 627 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3071 3314 3461 3655 3736 Size: 325 217 627 1284 243 147 194 81 Cuts at: 3736 5191 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1284 627 325 243 217 194 147 81 BanII G_rGCy'C Cuts at: 1391 2037 3083 3734 1391 Size: 646 1046 651 4072 Fragments arranged by size: 4072 1046 651 646 BbeI G_GCGC'C Cuts at: 3075 3465 3075 Size: 390 6025 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2993 3420 3711 928 Size: 971 1094 427 291 3632 Fragments arranged by size: 3632 1094 971 427 291 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4606 4640 1903 Size: 34 2669 34 3678 Fragments arranged by size: 3678 2669 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4307 5517 4307 Size: 1210 5205 BclI T'GATC_A Cuts at: 1548 1548 Size: 6415 BfrBI ATG'CAT Cuts at: 2813 2813 Size: 6415 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3087 5026 136 Size: 107 122 71 631 605 1415 1939 1525 Fragments arranged by size: 1939 1525 1415 631 605 122 107 71 BglII A'GATC_T Cuts at: 6410 6410 Size: 6415 BlpI GC'TnA_GC Cuts at: 3832 3832 Size: 6415 Bme1580I G_kGCm'C Cuts at: 1790 3083 4476 5632 1790 Size: 1293 1393 1156 2573 Fragments arranged by size: 2573 1393 1293 1156 BmgBI CAC'GTC Cuts at: 1318 3249 1318 Size: 1931 4484 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4146 5104 442 Size: 416 53 83 399 24 2729 958 1753 Fragments arranged by size: 2729 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6415 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2496 2496 Size: 6415 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2469 2646 3195 3405 5075 1239 Size: 1230 177 549 210 1670 2579 Fragments arranged by size: 2579 1670 1230 549 210 177 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2314 2630 3005 4540 5405 5703 2314 Size: 316 375 1535 865 298 3026 Fragments arranged by size: 3026 1535 865 375 316 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5078 882 Size: 617 3579 2219 Fragments arranged by size: 3579 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2110 2244 3712 4206 493 Size: 711 906 134 1468 494 2702 Fragments arranged by size: 2702 1468 906 711 494 134 BsaBI GATnn'nnATC Cuts at: 6409 6409 Size: 6415 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3072 3376 3462 4222 Size: 53 83 186 936 1539 304 86 760 Cuts at: 4222 4604 5239 275 Size: 382 635 1451 Fragments arranged by size: 1539 1451 936 760 635 382 304 186 86 83 53 BsaWI w'CCGG_w Cuts at: 4848 5246 5520 5667 6078 4848 Size: 398 274 147 411 5185 Fragments arranged by size: 5185 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2442 2462 2472 2492 2613 Size: 30 165 30 1039 20 10 20 121 Cuts at: 2613 2643 1178 Size: 30 4950 Fragments arranged by size: 4950 1039 165 121 30 30 30 20 20 10 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2128 2627 2696 1186 Size: 942 499 69 4905 Fragments arranged by size: 4905 942 499 69 BseYI C'CCAG_C Cuts at: 5618 6114 5618 Size: 496 5919 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2653 3255 1163 Size: 573 917 602 4323 Fragments arranged by size: 4323 917 602 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2030 3865 4626 5654 1540 Size: 275 186 29 1835 761 1028 2301 Fragments arranged by size: 2301 1835 1028 761 275 186 29 BsiHKAI G_wGCw'C Cuts at: 2241 4476 4561 5632 2241 Size: 2235 85 1071 3024 Fragments arranged by size: 3024 2235 1071 85 BsmI GAATG_Cn' Cuts at: 3926 4019 3926 Size: 93 6322 BsmBI CGTCTCn'nnnn_ Cuts at: 3367 3677 3367 Size: 310 6105 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2241 3083 3734 4476 4561 5632 Size: 399 247 204 842 651 742 85 1071 Cuts at: 5632 1391 Size: 2174 Fragments arranged by size: 2174 1071 842 742 651 399 247 204 85 BspEI T'CCGG_A Cuts at: 6078 6078 Size: 6415 BspHI T'CATG_A Cuts at: 2482 4304 6034 2482 Size: 1822 1730 2863 Fragments arranged by size: 2863 1822 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3222 3770 844 Size: 2378 548 3489 Fragments arranged by size: 3489 2378 548 BsrBI CCG'CTC Cuts at: 1394 1823 4302 1394 Size: 429 2479 3507 Fragments arranged by size: 3507 2479 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4910 5084 65 Size: 1183 3662 174 1396 Fragments arranged by size: 3662 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2147 5059 5246 1700 Size: 110 337 2912 187 2869 Fragments arranged by size: 2912 2869 337 187 110 BsrGI T'GTAC_A Cuts at: 96 2400 3337 96 Size: 2304 937 3174 Fragments arranged by size: 3174 2304 937 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6415 BssSI C'ACGA_G Cuts at: 4475 5487 4475 Size: 1012 5403 BstAPI GCAn_nnn'nTGC Cuts at: 794 2781 3242 794 Size: 1987 461 3967 Fragments arranged by size: 3967 1987 461 BstBI TT'CG_AA Cuts at: 2007 5219 2007 Size: 3212 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6415 BstYI r'GATC_y Cuts at: 1972 2154 2987 3118 3745 4112 4497 4514 5966 Size: 182 833 131 627 367 385 17 1452 Cuts at: 5966 6052 6064 6410 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 833 627 385 367 346 182 131 86 17 12 BstZ17I GTA'TAC Cuts at: 2508 2508 Size: 6415 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5863 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5676 Fragments arranged by size: 5676 683 56 BtsI GCAGTG_nn' Cuts at: 3243 3931 4725 4745 3243 Size: 688 794 20 4913 Fragments arranged by size: 4913 794 688 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6415 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6380 DraI TTT'AAA Cuts at: 3597 3719 3597 Size: 122 6293 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5422 809 Size: 507 4106 1802 Fragments arranged by size: 4106 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2027 2149 2429 Size: 54 1425 50 275 18 197 122 280 Cuts at: 2429 3862 4751 6380 8 Size: 1433 889 1629 43 Fragments arranged by size: 1629 1433 1425 889 280 275 197 122 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2027 3862 1537 Size: 275 215 1835 4090 Fragments arranged by size: 4090 1835 275 215 EarI CTCTTCn'nnn_ Cuts at: 1353 4345 1353 Size: 2992 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5002 5376 5522 387 Size: 1064 138 3413 374 146 1280 Fragments arranged by size: 3413 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2469 2646 3027 3195 3405 3503 3653 Size: 393 837 177 381 168 210 98 150 Cuts at: 3653 4478 5075 5862 6130 1239 Size: 825 597 787 268 1524 Fragments arranged by size: 1524 837 825 787 597 393 381 268 210 177 168 150 98 EcoO109I rG'GnC_Cy Cuts at: 2937 3080 3619 3672 2937 Size: 143 539 53 5680 Fragments arranged by size: 5680 539 143 53 EcoRI G'AATT_C Cuts at: 2746 3724 4102 2746 Size: 978 378 5059 Fragments arranged by size: 5059 978 378 EcoRV GAT'ATC Cuts at: 2279 2279 Size: 6415 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3058 3599 3058 Size: 541 5874 FspI TGC'GCA Cuts at: 4921 4921 Size: 6415 HaeII r_GCGC'y Cuts at: 1678 2124 3075 3465 5562 1678 Size: 446 951 390 2097 2531 Fragments arranged by size: 2531 2097 951 446 390 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2342 2374 2462 2494 5056 Size: 32 135 32 770 32 88 32 2562 Cuts at: 5056 5088 5130 5162 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2562 770 135 88 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3759 4005 669 Size: 988 281 1821 246 3079 Fragments arranged by size: 3079 1821 988 281 246 HindIII A'AGCT_T Cuts at: 748 2205 748 Size: 1457 4958 HpaI GTT'AAC Cuts at: 4005 4005 Size: 6415 KasI G'GCGC_C Cuts at: 3071 3461 3071 Size: 390 6025 KpnI G_GTAC'C Cuts at: 3659 3740 3659 Size: 81 6334 MfeI C'AATT_G Cuts at: 4014 4014 Size: 6415 MluI A'CGCG_T Cuts at: 6291 6291 Size: 6415 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3490 5529 5713 1305 Size: 2185 2039 184 2007 Fragments arranged by size: 2185 2039 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4593 Fragments arranged by size: 4593 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2246 2960 3287 4373 4732 4891 518 Size: 648 1080 714 327 1086 359 159 2042 Fragments arranged by size: 2042 1086 1080 714 648 359 327 159 MspA1I CmG'CkG Cuts at: 1606 3631 3831 4508 5656 5901 1606 Size: 2025 200 677 1148 245 2120 Fragments arranged by size: 2120 2025 1148 677 245 200 NaeI GCC'GGC Cuts at: 2149 2149 Size: 6415 NarI GG'CG_CC Cuts at: 3072 3462 3072 Size: 390 6025 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5863 NdeI CA'TA_TG Cuts at: 387 387 Size: 6415 NgoMIV G'CCGG_C Cuts at: 2147 2147 Size: 6415 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6415 NotI GC'GGCC_GC Cuts at: 3862 3862 Size: 6415 NsiI A_TGCA'T Cuts at: 2815 2815 Size: 6415 NspI r_CATG'y Cuts at: 1132 1229 3448 3773 5318 1132 Size: 97 2219 325 1545 2229 Fragments arranged by size: 2229 2219 1545 325 97 PasI CC'CwG_GG Cuts at: 1348 3076 1348 Size: 1728 4687 PciI A'CATG_T Cuts at: 5314 5314 Size: 6415 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6415 PmeI GTTT'AAAC Cuts at: 3719 3719 Size: 6415 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3211 3243 4484 4516 5997 6029 3211 Size: 32 1241 32 1481 32 3597 Fragments arranged by size: 3597 1481 1241 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3619 3619 Size: 6415 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6415 PsiI TTA'TAA Cuts at: 2490 2534 3985 2490 Size: 44 1451 4920 Fragments arranged by size: 4920 1451 44 PspOMI G'GGCC_C Cuts at: 3079 3079 Size: 6415 PstI C_TGCA'G Cuts at: 830 2128 3767 830 Size: 1298 1639 3478 Fragments arranged by size: 3478 1639 1298 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6415 PvuII CAG'CTG Cuts at: 3631 3631 Size: 6415 SalI G'TCGA_C Cuts at: 1936 3757 1936 Size: 1821 4594 SbfI CC_TGCA'GG Cuts at: 3767 3767 Size: 6415 ScaI AGT'ACT Cuts at: 2638 3107 4663 6375 2638 Size: 469 1556 1712 2678 Fragments arranged by size: 2678 1712 1556 469 SexAI A'CCwGG_T Cuts at: 3359 3359 Size: 6415 SfcI C'TryA_G Cuts at: 826 1045 2124 2437 3264 3763 4898 5579 5770 Size: 219 1079 313 827 499 1135 681 191 Cuts at: 5770 826 Size: 1471 Fragments arranged by size: 1471 1135 1079 827 681 499 313 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3087 3087 Size: 6415 SfoI GGC'GCC Cuts at: 3073 3463 3073 Size: 390 6025 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6415 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6415 SmaI CCC'GGG Cuts at: 1872 3742 1872 Size: 1870 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2293 2609 3020 4519 5420 5682 Size: 197 926 350 316 411 1499 901 262 Cuts at: 5682 820 Size: 1553 Fragments arranged by size: 1553 1499 926 901 411 350 316 262 197 SnaBI TAC'GTA Cuts at: 493 3712 4206 493 Size: 3219 494 2702 Fragments arranged by size: 3219 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6415 SphI G_CATG'C Cuts at: 1132 3773 1132 Size: 2641 3774 SspI AAT'ATT Cuts at: 5 52 4339 5 Size: 47 4287 2081 Fragments arranged by size: 4287 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6415 StyI C'CwwG_G Cuts at: 513 1065 3322 3854 513 Size: 552 2257 532 3074 Fragments arranged by size: 3074 2257 552 532 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4442 4459 4612 4797 5242 325 Size: 1255 2862 17 153 185 445 1498 Fragments arranged by size: 2862 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2400 2636 3105 Size: 274 80 33 51 1443 423 236 469 Cuts at: 3105 3337 4661 6373 96 Size: 232 1324 1712 138 Fragments arranged by size: 1712 1443 1324 469 423 274 236 232 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2442 2474 2613 2645 3481 3513 6375 6407 2442 Size: 32 139 32 836 32 2862 32 2450 Fragments arranged by size: 2862 2450 836 139 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6415 XbaI T'CTAG_A Cuts at: 3751 3751 Size: 6415 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6415 XmaI C'CCGG_G Cuts at: 1870 3740 1870 Size: 1870 4545 XmnI GAAnn'nnTTC Cuts at: 3223 4544 3223 Size: 1321 5094 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4223 276 Size: 53 83 186 936 2689 2468 Fragments arranged by size: 2689 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbvCI Bpu10I BsiWI BstXI Bsu36I DraIII EcoICRI EcoNI FseI FspAI NruI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SrfI SwaI XcmI