Restriction map for
pFN21ASDA0525
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII AgeI AscI AseI AsnI
AspI AviII AvrII BalI BamHI
BbrPI BbvCI BcgI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII Cfr10I CpoI
DrdI EaeI EagI EclXI Eco52I
EcoO65I EcoRI FauI FbaI FseI
FspI HpaI KspI MamI MluI
MluNI MroI MscI MspA1I MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SapI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI SspI SwaI Tth111I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 1679 GGTACC G^GTACC
AcyI 1663 GGCGCC G[GA]^CG[CT]C
AhdI 937 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2209 GTGCAC G^TGCAC
Aor51HI 1357 AGCGCT AGC^GCT
ApaI 2324 GGGCCC GGGCC^C
ApaLI 2209 GTGCAC G^TGCAC
Asp700I 2434 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1679 GGTACC G^GTACC
AspEI 937 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 406 CCCGAG C^[CT]CG[GA]G
BbeI 1663 GGCGCC GGCGC^C
BglII 918 AGATCT A^GATCT
BsaHI 1663 GGCGCC G[GA]^CG[CT]C
BseRI 2197 CTCCTC GAGGAG(10/8)
BsoBI 406 CCCGAG C^[CT]CG[GA]G
BspDI 1733 ATCGAT AT^CGAT
BsrDI 1219 GCAATG GCAATG(2/0)
ClaI 1733 ATCGAT AT^CGAT
DraIII 1249 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 477 CCACGG C^C[GA][CT]GG
Eam1105I 937 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1357 AGCGCT AGC^GCT
EcoNI 318 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 303 GATATC GAT^ATC
EcoT22I 1925 ATGCAT ATGCA^T
Hin1I 1663 GGCGCC G[GA]^CG[CT]C
KasI 1663 GGCGCC G^GCGCC
KpnI 1679 GGTACC GGTAC^C
MfeI 2732 CAATTG C^AATTG
MunI 2732 CAATTG C^AATTG
NarI 1663 GGCGCC GG^CGCC
NsiI 1925 ATGCAT ATGCA^T
NspI 1689 GCATGT [GA]CATG^[CT]
PflMI 1056 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI 2293 GTCGAC G^TCGAC
SbfI 357 CCTGCAGG CCTGCA^GG
ScaI 2383 AGTACT AGT^ACT
SexAI 1085 ACCTGGT A^CC[AT]GGT
SfoI 1663 GGCGCC GGC^GCC
SmlI 2280 CTTGAG C^T[CT][GA]AG
Sse8387I 357 CCTGCAGG CCTGCA^GG
Van91I 1056 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2434 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1257 GTCTAC GT^[AC][GT]AC
2293 GTCGAC
AciI 856 GCGG CCGC(-3/-1)
2181 GCGG
AlwNI 361 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1043 CAGTGACTG
AocI 318 CCTCAGG CC^T[ACGT]AGG
379 CCTCAGG
BbsI 697 GAAGAC GAAGAC(2/6)
1014 GTCTTC
BcnI 1287 CCGGG CC^[GC]GG
1668 CCCGG
BpmI 1393 CTCCAG CTGGAG(16/14)
332 CTGGAG
BpuAI 697 GAAGAC GAAGAC(2/6)
1014 GTCTTC
BstYI 918 AGATCT [GA]^GATC[CT]
1283 AGATCC
Bsu36I 318 CCTCAGG CC^T[ACGT]AGG
379 CCTCAGG
CfoI 1358 GCGC GCG^C
1664 GCGC
Eco81I 318 CCTCAGG CC^T[ACGT]AGG
379 CCTCAGG
HaeII 1357 AGCGCT [GA]GCGC^[CT]
1663 GGCGCC
HapII 1287 CCGG C^CGG
1669 CCGG
HhaI 1358 GCGC GCG^C
1664 GCGC
HinP1I 1358 GCGC G^CGC
1664 GCGC
HincII 1502 GTCAAC GT[CT]^[GA]AC
2293 GTCGAC
HindII 1502 GTCAAC GT[CT]^[GA]AC
2293 GTCGAC
HpaII 1287 CCGG C^CGG
1669 CCGG
MflI 918 AGATCT [GA]^GATC[CT]
1283 AGATCC
MspI 1287 CCGG C^CGG
1669 CCGG
NciI 1287 CCGGG CC^[GC]GG
1668 CCCGG
StuI 1967 AGGCCT AGG^CCT
2099 AGGCCT
TaqI 1734 TCGA T^CGA
2294 TCGA
TthHB8I 1734 TCGA T^CGA
2294 TCGA
XbaI 867 TCTAGA T^CTAGA
1495 TCTAGA
XhoII 918 AGATCT [GA]^GATC[CT]
1283 AGATCC
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI AvaII BanI BanII BbvI
BciVI BfaI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspMI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HgaI HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI