(Circular) (Six-base) MAPSORT of: pFN21ASDA0525.seq Check: 7560 from: 1 to: 7573 pFN21ASDA0525 7573 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2328 2328 Size: 7573 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5383 278 Size: 53 83 186 936 3847 2468 Fragments arranged by size: 3847 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3281 4317 4916 1937 Size: 1344 1036 599 4594 Fragments arranged by size: 4594 1344 1036 599 Acc65I G'GTAC_C Cuts at: 3702 4894 3702 Size: 1192 6381 AclI AA'CG_TT Cuts at: 1563 5700 6073 1563 Size: 4137 373 3063 Fragments arranged by size: 4137 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2148 2727 4690 4869 5636 7020 7288 1632 Size: 516 579 1963 179 767 1384 268 1917 Fragments arranged by size: 1963 1917 1384 767 579 516 268 179 AfeI AGC'GCT Cuts at: 3382 3382 Size: 7573 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7376 AflIII A'CryG_T Cuts at: 1203 6472 7449 1203 Size: 5269 977 1327 Fragments arranged by size: 5269 1327 977 AgeI A'CCGG_T Cuts at: 6404 6404 Size: 7573 AhdI GACnn_n'nnGTC Cuts at: 2965 6302 2965 Size: 3337 4236 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7573 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3493 1184 Size: 2309 5264 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4203 4235 4203 Size: 32 7541 AlwNI CAG_nnn'CTG Cuts at: 2389 3071 5316 6888 2389 Size: 682 2245 1572 3074 Fragments arranged by size: 3074 2245 1572 682 ApaI G_GGCC'C Cuts at: 4351 4351 Size: 7573 ApaLI G'TGCA_C Cuts at: 4232 5630 6786 4232 Size: 1398 1156 5019 Fragments arranged by size: 5019 1398 1156 ApoI r'AATT_y Cuts at: 1493 2894 3161 3262 3818 4427 4524 4795 4882 Size: 1401 267 101 556 609 97 271 87 Cuts at: 4882 5108 5260 7369 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1401 609 556 271 267 226 152 101 97 87 AseI AT'TA_AT Cuts at: 160 1032 6127 160 Size: 872 5095 1606 Fragments arranged by size: 5095 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7573 AvaI C'yCGr_G Cuts at: 1870 1943 2429 4898 1870 Size: 73 486 2469 4545 Fragments arranged by size: 4545 2469 486 73 BamHI G'GATC_C Cuts at: 4903 5270 4903 Size: 367 7206 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2658 3686 3702 4894 6349 Size: 325 217 627 871 1028 16 1192 1455 Cuts at: 6349 618 Size: 1842 Fragments arranged by size: 1842 1455 1192 1028 871 627 325 217 16 BanII G_rGCy'C Cuts at: 1391 2436 2451 3680 4184 4351 4892 1391 Size: 1045 15 1229 504 167 541 4072 Fragments arranged by size: 4072 1229 1045 541 504 167 15 BbeI G_GCGC'C Cuts at: 3690 3690 Size: 7573 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2727 3030 928 Size: 971 828 303 5471 Fragments arranged by size: 5471 971 828 303 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5764 5798 1903 Size: 34 3827 34 3678 Fragments arranged by size: 3827 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2541 2934 4550 4859 5465 6675 2541 Size: 393 1616 309 606 1210 3439 Fragments arranged by size: 3439 1616 1210 606 393 309 BclI T'GATC_A Cuts at: 1548 1548 Size: 7573 BfrBI ATG'CAT Cuts at: 3950 3950 Size: 7573 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6184 136 Size: 107 122 71 631 605 4512 1525 Fragments arranged by size: 4512 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2941 7568 2941 Size: 4627 2946 BlpI GC'TnA_GC Cuts at: 4990 4990 Size: 7573 Bme1580I G_kGCm'C Cuts at: 1790 4236 4351 5634 6790 1790 Size: 2446 115 1283 1156 2573 Fragments arranged by size: 2573 2446 1283 1156 115 BmgBI CAC'GTC Cuts at: 1318 4321 1318 Size: 3003 4570 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2195 2995 3708 Size: 416 53 83 399 24 778 800 713 Cuts at: 3708 4544 5304 6262 442 Size: 836 760 958 1753 Fragments arranged by size: 1753 958 836 800 778 760 713 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7573 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2376 3401 6233 1239 Size: 1137 1025 2832 2579 Fragments arranged by size: 2832 2579 1137 1025 Bpu10I CC'TnA_GC Cuts at: 3365 4142 3365 Size: 777 6796 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4324 5698 6563 6861 4324 Size: 1374 865 298 5036 Fragments arranged by size: 5036 1374 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6236 882 Size: 617 4737 2219 Fragments arranged by size: 4737 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4870 5364 493 Size: 711 3666 494 2702 Fragments arranged by size: 3666 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7567 7567 Size: 7573 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3687 5380 5762 6397 Size: 53 83 186 936 2154 1693 382 635 Cuts at: 6397 275 Size: 1451 Fragments arranged by size: 2154 1693 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6006 6404 6678 6825 7236 6006 Size: 398 274 147 411 6343 Fragments arranged by size: 6343 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2805 2835 3161 3191 3399 Size: 30 165 30 1402 30 326 30 208 Cuts at: 3399 3429 4203 4233 1178 Size: 30 774 30 4518 Fragments arranged by size: 4518 1402 774 326 208 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4211 1186 Size: 3025 4548 BseYI C'CCAG_C Cuts at: 4365 6776 7272 4365 Size: 2411 496 4666 Fragments arranged by size: 4666 2411 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7000 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5023 5784 6812 1540 Size: 275 186 3022 761 1028 2301 Fragments arranged by size: 3022 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2126 2753 3666 3789 4236 4358 5634 5719 6790 Size: 627 913 123 447 122 1276 85 1071 Cuts at: 6790 2126 Size: 2909 Fragments arranged by size: 2909 1276 1071 913 627 447 123 122 85 BsmI GAATG_Cn' Cuts at: 5084 5177 5084 Size: 93 7480 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2126 2436 2451 2753 3666 3680 3789 Size: 399 336 310 15 302 913 14 109 Cuts at: 3789 4184 4236 4351 4358 4892 5634 5719 6790 Size: 395 52 115 7 534 742 85 1071 Cuts at: 6790 1391 Size: 2174 Fragments arranged by size: 2174 1071 913 742 534 399 395 336 310 302 115 109 85 52 15 14 7 BspEI T'CCGG_A Cuts at: 7236 7236 Size: 7573 BspHI T'CATG_A Cuts at: 5462 7192 5462 Size: 1730 5843 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2328 2374 2620 4928 844 Size: 1484 46 246 2308 3489 Fragments arranged by size: 3489 2308 1484 246 46 BsrBI CCG'CTC Cuts at: 1394 1823 5460 1394 Size: 429 3637 3507 Fragments arranged by size: 3637 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3249 6068 6242 65 Size: 1183 2001 2819 174 1396 Fragments arranged by size: 2819 2001 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6217 6404 1700 Size: 110 4407 187 2869 Fragments arranged by size: 4407 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3406 3646 4603 96 Size: 3310 240 957 3066 Fragments arranged by size: 3310 3066 957 240 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7573 BssSI C'ACGA_G Cuts at: 5633 6645 5633 Size: 1012 6561 BstAPI GCAn_nnn'nTGC Cuts at: 794 2864 2873 4201 4337 794 Size: 2070 9 1328 136 4030 Fragments arranged by size: 4030 2070 1328 136 9 BstBI TT'CG_AA Cuts at: 2007 6377 2007 Size: 4370 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7573 BstXI CCAn_nnnn'nTGG Cuts at: 2166 3101 3166 4569 2166 Size: 935 65 1403 5170 Fragments arranged by size: 5170 1403 935 65 BstYI r'GATC_y Cuts at: 1972 2941 3306 4903 5270 5655 5672 7124 7210 Size: 969 365 1597 367 385 17 1452 86 Cuts at: 7210 7222 7568 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1597 1452 969 385 367 365 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2342 2403 2342 Size: 61 7512 BtgI C'CryG_G Cuts at: 513 1065 2022 2500 513 Size: 552 957 478 5586 Fragments arranged by size: 5586 957 552 478 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6834 Fragments arranged by size: 6834 683 56 BtsI GCAGTG_nn' Cuts at: 2417 2574 3596 4338 5089 5883 5903 2417 Size: 157 1022 742 751 794 20 4087 Fragments arranged by size: 4087 1022 794 751 742 157 20 ClaI AT'CG_AT Cuts at: 1554 3757 1554 Size: 2203 5370 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7538 DraI TTT'AAA Cuts at: 3261 3394 3894 4877 3261 Size: 133 500 983 5957 Fragments arranged by size: 5957 983 500 133 DraIII CAC_nnn'GTG Cuts at: 3277 3277 Size: 7573 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6580 809 Size: 507 5264 1802 Fragments arranged by size: 5264 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5020 5909 7538 Size: 54 1425 50 275 18 3190 889 1629 Cuts at: 7538 8 Size: 43 Fragments arranged by size: 3190 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5020 1537 Size: 275 3208 4090 Fragments arranged by size: 4090 3208 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2641 2892 3795 4683 5503 1353 Size: 1288 251 903 888 820 3423 Fragments arranged by size: 3423 1288 903 888 820 251 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6160 6534 6680 387 Size: 1064 138 4571 374 146 1280 Fragments arranged by size: 4571 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2148 2376 2727 3401 4690 4869 5636 Size: 393 516 228 351 674 1289 179 767 Cuts at: 5636 6233 7020 7288 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1289 787 767 674 597 516 393 351 268 228 179 EcoNI CCTnn'n_nnAGG Cuts at: 2345 2345 Size: 7573 EcoO109I rG'GnC_Cy Cuts at: 2386 3451 4147 4347 2386 Size: 1065 696 200 5612 Fragments arranged by size: 5612 1065 696 200 EcoRI G'AATT_C Cuts at: 4882 5260 4882 Size: 378 7195 EcoRV GAT'ATC Cuts at: 2328 2328 Size: 7573 FspI TGC'GCA Cuts at: 6079 6079 Size: 7573 HaeII r_GCGC'y Cuts at: 1678 3384 3690 6720 1678 Size: 1706 306 3030 2531 Fragments arranged by size: 3030 2531 1706 306 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2351 2383 2740 2771 2772 Size: 32 135 32 779 32 357 31 1 Cuts at: 2772 2803 2951 2983 3161 3193 4166 4198 6214 Size: 31 148 32 178 32 973 32 2016 Cuts at: 6214 6246 6288 6320 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2016 973 779 357 178 148 135 42 32 32 32 32 32 32 32 32 31 31 1 HincII GTy'rAC Cuts at: 669 1657 1938 3527 4318 4917 5163 669 Size: 988 281 1589 791 599 246 3079 Fragments arranged by size: 3079 1589 988 791 599 281 246 HindIII A'AGCT_T Cuts at: 748 3053 4460 4483 4832 748 Size: 2305 1407 23 349 3489 Fragments arranged by size: 3489 2305 1407 349 23 HpaI GTT'AAC Cuts at: 5163 5163 Size: 7573 KasI G'GCGC_C Cuts at: 3686 3686 Size: 7573 KpnI G_GTAC'C Cuts at: 3706 4898 3706 Size: 1192 6381 MfeI C'AATT_G Cuts at: 4755 5172 4755 Size: 417 7156 MluI A'CGCG_T Cuts at: 7449 7449 Size: 7573 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3190 3768 4763 4771 6687 6871 1305 Size: 1885 578 995 8 1916 184 2007 Fragments arranged by size: 2007 1916 1885 995 578 184 8 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5751 Fragments arranged by size: 5751 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2730 3065 3629 4646 5531 5890 6049 Size: 648 1564 335 564 1017 885 359 159 Cuts at: 6049 518 Size: 2042 Fragments arranged by size: 2042 1564 1017 885 648 564 359 335 159 MspA1I CmG'CkG Cuts at: 1606 4989 5666 6814 7059 1606 Size: 3383 677 1148 245 2120 Fragments arranged by size: 3383 2120 1148 677 245 NarI GG'CG_CC Cuts at: 3687 3687 Size: 7573 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 6064 Fragments arranged by size: 6064 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7573 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7573 NotI GC'GGCC_GC Cuts at: 5020 5020 Size: 7573 NsiI A_TGCA'T Cuts at: 3952 3952 Size: 7573 NspI r_CATG'y Cuts at: 1132 1229 3716 4931 6476 1132 Size: 97 2487 1215 1545 2229 Fragments arranged by size: 2487 2229 1545 1215 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7573 PciI A'CATG_T Cuts at: 6472 6472 Size: 7573 PflMI CCAn_nnn'nTGG Cuts at: 1108 3085 1108 Size: 1977 5596 PfoI T'CCnGG_A Cuts at: 2388 3309 2388 Size: 921 6652 PmeI GTTT'AAAC Cuts at: 4877 4877 Size: 7573 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4203 4235 5642 5674 7155 7187 4203 Size: 32 1407 32 1481 32 4589 Fragments arranged by size: 4589 1481 1407 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2386 3451 4147 2386 Size: 1065 696 5812 Fragments arranged by size: 5812 1065 696 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7573 PsiI TTA'TAA Cuts at: 5143 5143 Size: 7573 PspOMI G'GGCC_C Cuts at: 4347 4347 Size: 7573 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2532 2564 2532 Size: 32 7541 PstI C_TGCA'G Cuts at: 830 2325 2385 2581 3603 4442 4925 830 Size: 1495 60 196 1022 839 483 3478 Fragments arranged by size: 3478 1495 1022 839 483 196 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7573 SalI G'TCGA_C Cuts at: 1936 4316 4915 1936 Size: 2380 599 4594 Fragments arranged by size: 4594 2380 599 SbfI CC_TGCA'GG Cuts at: 2385 4925 2385 Size: 2540 5033 ScaI AGT'ACT Cuts at: 4408 5821 7533 4408 Size: 1413 1712 4448 Fragments arranged by size: 4448 1712 1413 SexAI A'CCwGG_T Cuts at: 3108 3108 Size: 7573 SfcI C'TryA_G Cuts at: 826 1045 2321 2381 2521 2577 3483 3599 4438 Size: 219 1276 60 140 56 906 116 839 Cuts at: 4438 4921 6056 6737 6928 826 Size: 483 1135 681 191 1471 Fragments arranged by size: 1471 1276 1135 906 839 681 483 219 191 140 116 60 56 SfoI GGC'GCC Cuts at: 3688 3688 Size: 7573 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7573 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7573 SmaI CCC'GGG Cuts at: 1872 4900 1872 Size: 3028 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4303 5677 6578 6840 820 Size: 197 926 2360 1374 901 262 1553 Fragments arranged by size: 2360 1553 1374 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4870 5364 493 Size: 4377 494 2702 Fragments arranged by size: 4377 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7573 SphI G_CATG'C Cuts at: 1132 4931 1132 Size: 3799 3774 SspI AAT'ATT Cuts at: 5 52 5497 5 Size: 47 5445 2081 Fragments arranged by size: 5445 2081 47 StuI AGG'CCT Cuts at: 1517 3992 4124 1517 Size: 2475 132 4966 Fragments arranged by size: 4966 2475 132 StyI C'CwwG_G Cuts at: 513 1065 2022 2165 2529 2654 3993 4326 5012 Size: 552 957 143 364 125 1339 333 686 Cuts at: 5012 513 Size: 3074 Fragments arranged by size: 3074 1339 957 686 552 364 333 143 125 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5600 5617 5770 5955 6400 325 Size: 1255 4020 17 153 185 445 1498 Fragments arranged by size: 4020 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3406 3646 4008 Size: 274 80 33 51 1443 1429 240 362 Cuts at: 4008 4406 4603 5819 7531 96 Size: 398 197 1216 1712 138 Fragments arranged by size: 1712 1443 1429 1216 398 362 274 240 197 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7533 7565 7533 Size: 32 7541 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7573 XbaI T'CTAG_A Cuts at: 2890 3518 4909 2890 Size: 628 1391 5554 Fragments arranged by size: 5554 1391 628 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2680 3098 4639 2680 Size: 418 1541 5614 Fragments arranged by size: 5614 1541 418 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7573 XmaI C'CCGG_G Cuts at: 1870 4898 1870 Size: 3028 4545 XmnI GAAnn'nnTTC Cuts at: 4461 5702 4461 Size: 1241 6332 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5381 276 Size: 53 83 186 936 3847 2468 Fragments arranged by size: 3847 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstZ17I EcoICRI FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PvuII RsrII SacI SacII SanDI SapI SfiI SrfI SwaI