Restriction map for
pFN21ASDA0525
(Linear) (Six-base) MAPSORT of: KIAA0525.seq Check: 9102 from: 1 to: 2844
KIAA0525.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 305 2844
Size: 305 2539
AccI GT'mk_AC
Cuts at: 0 1258 2294 2844
Size: 1258 1036 550
Fragments arranged by size:
1258 1036 550
Acc65I G'GTAC_C
Cuts at: 0 1679 2844
Size: 1679 1165
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 704 2667 2844
Size: 125 579 1963 177
Fragments arranged by size:
1963 579 177 125
AfeI AGC'GCT
Cuts at: 0 1359 2844
Size: 1359 1485
AhdI GACnn_n'nnGTC
Cuts at: 0 942 2844
Size: 942 1902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1470 2844
Size: 1470 1374
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2180 2212 2844
Size: 2180 32 632
Fragments arranged by size:
2180 632 32
AlwNI CAG_nnn'CTG
Cuts at: 0 366 1048 2844
Size: 366 682 1796
Fragments arranged by size:
1796 682 366
ApaI G_GGCC'C
Cuts at: 0 2328 2844
Size: 2328 516
ApaLI G'TGCA_C
Cuts at: 0 2209 2844
Size: 2209 635
ApoI r'AATT_y
Cuts at: 0 871 1138 1239 1795 2404 2501 2772 2844
Size: 871 267 101 556 609 97 271 72
Fragments arranged by size:
871 609 556 271 267 101 97 72
AvaI C'yCGr_G
Cuts at: 0 406 2844
Size: 406 2438
BanI G'GyrC_C
Cuts at: 0 635 1663 1679 2844
Size: 635 1028 16 1165
Fragments arranged by size:
1165 1028 635 16
BanII G_rGCy'C
Cuts at: 0 413 428 1657 2161 2328 2844
Size: 413 15 1229 504 167 516
Fragments arranged by size:
1229 516 504 413 167 15
BbeI G_GCGC'C
Cuts at: 0 1667 2844
Size: 1667 1177
BbsI GAAGACnn'nnnn_
Cuts at: 0 704 1007 2844
Size: 704 303 1837
Fragments arranged by size:
1837 704 303
BciVI GTATCCnnnnn_n'
Cuts at: 0 518 911 2527 2844
Size: 518 393 1616 317
Fragments arranged by size:
1616 518 393 317
BfrBI ATG'CAT
Cuts at: 0 1927 2844
Size: 1927 917
BglII A'GATC_T
Cuts at: 0 918 2844
Size: 918 1926
Bme1580I G_kGCm'C
Cuts at: 0 2213 2328 2844
Size: 2213 115 516
Fragments arranged by size:
2213 516 115
BmgBI CAC'GTC
Cuts at: 0 2298 2844
Size: 2298 546
BmrI ACTGGGnnnn_n'
Cuts at: 0 172 972 1685 2521 2844
Size: 172 800 713 836 323
Fragments arranged by size:
836 800 713 323 172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 1378 2844
Size: 353 1025 1466
Fragments arranged by size:
1466 1025 353
Bpu10I CC'TnA_GC
Cuts at: 0 1342 2119 2844
Size: 1342 777 725
Fragments arranged by size:
1342 777 725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2301 2844
Size: 2301 543
BsaHI Gr'CG_yC
Cuts at: 0 1664 2844
Size: 1664 1180
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 782 812 1138 1168 1376 1406 2180 2210
Size: 782 30 326 30 208 30 774 30
Cuts at: 2210 2844
Size: 634
Fragments arranged by size:
782 774 634 326 208 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2188 2844
Size: 2188 656
BseYI C'CCAG_C
Cuts at: 0 2342 2844
Size: 2342 502
BsiHKAI G_wGCw'C
Cuts at: 0 103 730 1643 1766 2213 2335 2844
Size: 103 627 913 123 447 122 509
Fragments arranged by size:
913 627 509 447 123 122 103
Bsp1286I G_dGCh'C
Cuts at: 0 103 413 428 730 1643 1657 1766 2161
Size: 103 310 15 302 913 14 109 395
Cuts at: 2161 2213 2328 2335 2844
Size: 52 115 7 509
Fragments arranged by size:
913 509 395 310 302 115 109 103
52 15 14 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 305 351 597 2844
Size: 305 46 246 2247
Fragments arranged by size:
2247 305 246 46
BsrDI GCAATG_nn'
Cuts at: 0 1226 2844
Size: 1226 1618
BsrGI T'GTAC_A
Cuts at: 0 1383 1623 2580 2844
Size: 1383 240 957 264
Fragments arranged by size:
1383 957 264 240
BstAPI GCAn_nnn'nTGC
Cuts at: 0 841 850 2178 2314 2844
Size: 841 9 1328 136 530
Fragments arranged by size:
1328 841 530 136 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 143 1078 1143 2546 2844
Size: 143 935 65 1403 298
Fragments arranged by size:
1403 935 298 143 65
BstYI r'GATC_y
Cuts at: 0 918 1283 2844
Size: 918 365 1561
Fragments arranged by size:
1561 918 365
Bsu36I CC'TnA_GG
Cuts at: 0 319 380 2844
Size: 319 61 2464
Fragments arranged by size:
2464 319 61
BtgI C'CryG_G
Cuts at: 0 477 2844
Size: 477 2367
BtsI GCAGTG_nn'
Cuts at: 0 394 551 1573 2315 2844
Size: 394 157 1022 742 529
Fragments arranged by size:
1022 742 529 394 157
ClaI AT'CG_AT
Cuts at: 0 1734 2844
Size: 1734 1110
DraI TTT'AAA
Cuts at: 0 1238 1371 1871 2844
Size: 1238 133 500 973
Fragments arranged by size:
1238 973 500 133
DraIII CAC_nnn'GTG
Cuts at: 0 1254 2844
Size: 1254 1590
EarI CTCTTCn'nnn_
Cuts at: 0 618 869 1772 2660 2844
Size: 618 251 903 888 184
Fragments arranged by size:
903 888 618 251 184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 353 704 1378 2667 2844
Size: 125 228 351 674 1289 177
Fragments arranged by size:
1289 674 351 228 177 125
EcoNI CCTnn'n_nnAGG
Cuts at: 0 322 2844
Size: 322 2522
EcoO109I rG'GnC_Cy
Cuts at: 0 363 1428 2124 2324 2844
Size: 363 1065 696 200 520
Fragments arranged by size:
1065 696 520 363 200
EcoRV GAT'ATC
Cuts at: 0 305 2844
Size: 305 2539
HaeII r_GCGC'y
Cuts at: 0 1361 1667 2844
Size: 1361 306 1177
Fragments arranged by size:
1361 1177 306
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 328 360 717 748 749 780 928 960
Size: 328 32 357 31 1 31 148 32
Cuts at: 960 1138 1170 2143 2175 2844
Size: 178 32 973 32 669
Fragments arranged by size:
973 669 357 328 178 148 32 32
32 32 31 31 1
HincII GTy'rAC
Cuts at: 0 1504 2295 2844
Size: 1504 791 549
Fragments arranged by size:
1504 791 549
HindIII A'AGCT_T
Cuts at: 0 1030 2437 2460 2809 2844
Size: 1030 1407 23 349 35
Fragments arranged by size:
1407 1030 349 35 23
KasI G'GCGC_C
Cuts at: 0 1663 2844
Size: 1663 1181
KpnI G_GTAC'C
Cuts at: 0 1683 2844
Size: 1683 1161
MfeI C'AATT_G
Cuts at: 0 2732 2844
Size: 2732 112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1167 1745 2740 2748 2844
Size: 1167 578 995 8 96
Fragments arranged by size:
1167 995 578 96 8
MslI CAynn'nnrTG
Cuts at: 0 707 1042 1606 2623 2844
Size: 707 335 564 1017 221
Fragments arranged by size:
1017 707 564 335 221
NarI GG'CG_CC
Cuts at: 0 1664 2844
Size: 1664 1180
NsiI A_TGCA'T
Cuts at: 0 1929 2844
Size: 1929 915
NspI r_CATG'y
Cuts at: 0 1693 2844
Size: 1693 1151
PflMI CCAn_nnn'nTGG
Cuts at: 0 1062 2844
Size: 1062 1782
PfoI T'CCnGG_A
Cuts at: 0 365 1286 2844
Size: 365 921 1558
Fragments arranged by size:
1558 921 365
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2180 2212 2844
Size: 2180 32 632
Fragments arranged by size:
2180 632 32
PpuMI rG'GwC_Cy
Cuts at: 0 363 1428 2124 2844
Size: 363 1065 696 720
Fragments arranged by size:
1065 720 696 363
PspOMI G'GGCC_C
Cuts at: 0 2324 2844
Size: 2324 520
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 509 541 2844
Size: 509 32 2303
Fragments arranged by size:
2303 509 32
PstI C_TGCA'G
Cuts at: 0 302 362 558 1580 2419 2844
Size: 302 60 196 1022 839 425
Fragments arranged by size:
1022 839 425 302 196 60
SalI G'TCGA_C
Cuts at: 0 2293 2844
Size: 2293 551
SbfI CC_TGCA'GG
Cuts at: 0 362 2844
Size: 362 2482
ScaI AGT'ACT
Cuts at: 0 2385 2844
Size: 2385 459
SexAI A'CCwGG_T
Cuts at: 0 1085 2844
Size: 1085 1759
SfcI C'TryA_G
Cuts at: 0 298 358 498 554 1460 1576 2415 2844
Size: 298 60 140 56 906 116 839 429
Fragments arranged by size:
906 839 429 298 140 116 60 56
SfoI GGC'GCC
Cuts at: 0 1665 2844
Size: 1665 1179
SmlI C'TyrA_G
Cuts at: 0 2280 2844
Size: 2280 564
StuI AGG'CCT
Cuts at: 0 1969 2101 2844
Size: 1969 132 743
Fragments arranged by size:
1969 743 132
StyI C'CwwG_G
Cuts at: 0 142 506 631 1970 2303 2844
Size: 142 364 125 1339 333 541
Fragments arranged by size:
1339 541 364 333 142 125
TatI w'GTAC_w
Cuts at: 0 1383 1623 1985 2383 2580 2844
Size: 1383 240 362 398 197 264
Fragments arranged by size:
1383 398 362 264 240 197
XbaI T'CTAG_A
Cuts at: 0 867 1495 2844
Size: 867 628 1349
Fragments arranged by size:
1349 867 628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 657 1075 2616 2844
Size: 657 418 1541 228
Fragments arranged by size:
1541 657 418 228
XmnI GAAnn'nnTTC
Cuts at: 0 2438 2844
Size: 2438 406
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AhdI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI
BbsI BciVI BfrBI BglII Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsiHKAI Bsp1286I
BspMI BsrDI BsrGI BstAPI BstXI BstYI Bsu36I BtgI
BtsI ClaI DraI DraIII EarI Eco57MI EcoNI EcoO109I
EcoRV HaeII Hin4I HincII HindIII KasI KpnI MfeI
MmeI MslI NarI NsiI NspI PflMI PfoI PpiI
PpuMI PspOMI PsrI PstI SalI SbfI ScaI SexAI
SfcI SfoI SmlI StuI StyI TatI XbaI XcmI
XmnI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AleI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BclI BglI
BlpI BmtI BplI BsaI BsaAI BsaBI BsaWI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI
BssHII BssSI BstBI BstEII BstZ17I BtgZI CspCI DrdI
EaeI EagI EciI EcoICRI EcoRI FalI FseI FspI
FspAI HpaI MluI MscI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PmeI
PmlI PshAI PsiI PspXI PvuI PvuII RsrII SacI
SacII SanDI SapI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I
XhoI XmaI ZraI