Restriction map for
pFN21ASDA0517
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI AscI AspEI AspI
AvrII BbeI BbrPI BcgI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspEI
BspHI BspLU11I BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI HaeII Hin1I HindIII KasI
MamI MfeI MluI MroI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 1510 GGTACC G^GTACC
Alw44I 501 GTGCAC G^TGCAC
ApaLI 501 GTGCAC G^TGCAC
AseI 1581 ATTAAT AT^TAAT
AsnI 1581 ATTAAT AT^TAAT
Asp718I 1510 GGTACC G^GTACC
AviII 731 TGCGCA TGC^GCA
BamHI 2156 GGATCC G^GATCC
BanII 709 GAGCTC G[GA]GC[CT]^C
BbvCI 373 GCTGAGG CCTCAGC(-5/-2)
BciVI 804 GGATAC GTATCC(6/5)
BfaI 77 CTAG C^TAG
BglI 2225 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 880 GAGACC GGTCTC(1/5)
BspDI 2300 ATCGAT AT^CGAT
BspMI 1948 GCAGGT ACCTGC(4/8)
BsrBI 1407 GAGCGG CCGCTC(-3/-3)
BstAPI 149 GCAGTATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 2300 ATCGAT AT^CGAT
DraI 723 TTTAAA TTT^AAA
EcoRI 2041 GAATTC G^AATTC
FspI 731 TGCGCA TGC^GCA
HgaI 1661 GACGC GACGC(5/10)
HpaI 657 GTTAAC GTT^AAC
KpnI 1510 GGTACC GGTAC^C
KspI 958 CCGCGG CCGC^GG
MaeI 77 CTAG C^TAG
NaeI 55 GCCGGC GCC^GGC
NcoI 870 CCATGG C^CATGG
NgoMIV 55 GCCGGC G^CCGGC
NspI 1448 GCATGT [GA]CATG^[CT]
PshAI 882 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1581 ATTAAT AT^TAAT
SacI 709 GAGCTC GAGCT^C
SacII 958 CCGCGG CCGC^GG
SapI 625 GCTCTTC GCTCTTC(1/4)
SexAI 1909 ACCAGGT A^CC[AT]GGT
SfiI 2224 GGCCCCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1401 CCCGGG CCC^GGG
SmlI 541 CTCAAG C^T[CT][GA]AG
XcmI 2160 CCAGGTTTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1401 CCCGGG C^CCGGG
XmaI 1401 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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Asp700I 315 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
643 GAAATCATTC
BseRI 1761 CTCCTC GAGGAG(10/8)
376 GAGGAG
BsrDI 1725 CATTGC GCAATG(2/0)
1835 GCAATG
BsrFI 55 GCCGGC [GA]^CCGG[CT]
1159 ACCGGC
BstYI 1220 AGATCC [GA]^GATC[CT]
2156 GGATCC
Cfr10I 55 GCCGGC [GA]^CCGG[CT]
1159 ACCGGC
EarI 626 CTCTTC CTCTTC(1/4)
1383 GAAGAG
Ksp632I 626 CTCTTC CTCTTC(1/4)
1383 GAAGAG
MaeII 344 ACGT A^CGT
1415 ACGT
MflI 1220 AGATCC [GA]^GATC[CT]
2156 GGATCC
PflMI 2215 CCACTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2229 CCAAGGCCTGG
StuI 566 AGGCCT AGG^CCT
2232 AGGCCT
Van91I 2215 CCACTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2229 CCAAGGCCTGG
XhoII 1220 AGATCC [GA]^GATC[CT]
2156 GGATCC
XmnI 315 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
643 GAAATCATTC
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I