(Circular) (Six-base) MAPSORT of: pFN21ASDA0517.seq Check: 3745 from: 1 to: 7042 pFN21ASDA0517 7042 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4852 278 Size: 53 83 186 936 3316 2468 Fragments arranged by size: 3316 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4385 1937 Size: 2448 4594 Acc65I G'GTAC_C Cuts at: 3533 4363 3533 Size: 830 6212 AclI AA'CG_TT Cuts at: 1563 5169 5542 1563 Size: 3606 373 3063 Fragments arranged by size: 3606 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2636 3047 3554 4254 5105 6489 6757 1632 Size: 1004 411 507 700 851 1384 268 1917 Fragments arranged by size: 1917 1384 1004 851 700 507 411 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6845 AflIII A'CryG_T Cuts at: 1203 5941 6918 1203 Size: 4738 977 1327 Fragments arranged by size: 4738 1327 977 AgeI A'CCGG_T Cuts at: 5873 5873 Size: 7042 AhdI GACnn_n'nnGTC Cuts at: 5771 5771 Size: 7042 AleI CACnn'nnGTG Cuts at: 1166 2031 2429 3716 1166 Size: 865 398 1287 4492 Fragments arranged by size: 4492 1287 865 398 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2076 2780 4128 1184 Size: 892 704 1348 4098 Fragments arranged by size: 4098 1348 892 704 AlwNI CAG_nnn'CTG Cuts at: 2164 2426 4298 4785 6357 2164 Size: 262 1872 487 1572 2849 Fragments arranged by size: 2849 1872 1572 487 262 ApaLI G'TGCA_C Cuts at: 2524 5099 6255 2524 Size: 2575 1156 3311 Fragments arranged by size: 3311 2575 1156 ApoI r'AATT_y Cuts at: 1493 2483 3797 4064 4351 4577 4729 6838 1493 Size: 990 1314 267 287 226 152 2109 1697 Fragments arranged by size: 2109 1697 1314 990 287 267 226 152 AseI AT'TA_AT Cuts at: 160 1032 3605 5596 160 Size: 872 2573 1991 1606 Fragments arranged by size: 2573 1991 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7042 AvaI C'yCGr_G Cuts at: 1870 1943 2312 3047 3424 4367 1870 Size: 73 369 735 377 943 4545 Fragments arranged by size: 4545 943 735 377 369 73 BamHI G'GATC_C Cuts at: 4179 4372 4739 4179 Size: 193 367 6482 Fragments arranged by size: 6482 367 193 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2117 2238 3263 3533 4363 Size: 325 217 627 330 121 1025 270 830 Cuts at: 4363 5818 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1025 830 627 330 325 270 217 121 BanII G_rGCy'C Cuts at: 1391 2736 4361 1391 Size: 1345 1625 4072 Fragments arranged by size: 4072 1625 1345 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2397 2747 3516 928 Size: 971 498 350 769 4454 Fragments arranged by size: 4454 971 769 498 350 BbvCI CC'TCA_GC Cuts at: 2397 2397 Size: 7042 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5233 5267 1903 Size: 34 3296 34 3678 Fragments arranged by size: 3678 3296 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2821 4934 6144 2821 Size: 2113 1210 3719 Fragments arranged by size: 3719 2113 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7042 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4254 5653 136 Size: 107 122 71 631 605 2582 1399 1525 Fragments arranged by size: 2582 1525 1399 631 605 122 107 71 BglII A'GATC_T Cuts at: 7037 7037 Size: 7042 BlpI GC'TnA_GC Cuts at: 4459 4459 Size: 7042 Bme1580I G_kGCm'C Cuts at: 1790 2528 3867 5103 6259 1790 Size: 738 1339 1236 1156 2573 Fragments arranged by size: 2573 1339 1236 1156 738 BmgBI CAC'GTC Cuts at: 1318 2368 3439 1318 Size: 1050 1071 4921 Fragments arranged by size: 4921 1071 1050 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2279 3726 4174 Size: 416 53 83 399 24 862 1447 448 Cuts at: 4174 4773 5731 442 Size: 599 958 1753 Fragments arranged by size: 1753 1447 958 862 599 448 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7042 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2318 2367 2435 2582 2720 2798 2822 5702 Size: 1079 49 68 147 138 78 24 2880 Cuts at: 5702 1239 Size: 2579 Fragments arranged by size: 2880 2579 1079 147 138 78 68 49 24 Bpu10I CC'TnA_GC Cuts at: 2397 2397 Size: 7042 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2549 5167 6032 6330 2549 Size: 2618 865 298 3261 Fragments arranged by size: 3261 2618 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2897 5705 882 Size: 617 1398 2808 2219 Fragments arranged by size: 2808 2219 1398 617 BsaAI yAC'GTr Cuts at: 493 1204 4339 4833 493 Size: 711 3135 494 2702 Fragments arranged by size: 3135 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7036 7036 Size: 7042 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4849 5231 5866 275 Size: 53 83 186 936 3316 382 635 1451 Fragments arranged by size: 3316 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5475 5873 6147 6294 6705 5475 Size: 398 274 147 411 5812 Fragments arranged by size: 5812 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2023 2053 3708 3738 3834 Size: 30 165 30 620 30 1655 30 96 Cuts at: 3834 3864 1178 Size: 30 4356 Fragments arranged by size: 4356 1655 620 165 96 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2414 3775 1186 Size: 1228 1361 4453 Fragments arranged by size: 4453 1361 1228 BseYI C'CCAG_C Cuts at: 6245 6741 6245 Size: 496 6546 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2082 2204 3653 1163 Size: 573 346 122 1449 4552 Fragments arranged by size: 4552 1449 573 346 122 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4492 5253 6281 1540 Size: 275 186 2491 761 1028 2301 Fragments arranged by size: 2491 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2528 2541 2550 2736 3215 5103 5188 6259 2528 Size: 13 9 186 479 1888 85 1071 3311 Fragments arranged by size: 3311 1888 1071 479 186 85 13 9 BsmI GAATG_Cn' Cuts at: 4553 4646 4553 Size: 93 6949 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2528 2541 2550 2736 3215 3867 4361 Size: 399 738 13 9 186 479 652 494 Cuts at: 4361 5103 5188 6259 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 738 652 494 479 399 186 85 13 9 BspEI T'CCGG_A Cuts at: 6705 6705 Size: 7042 BspHI T'CATG_A Cuts at: 4931 6661 4931 Size: 1730 5312 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3962 4397 844 Size: 3118 435 3489 Fragments arranged by size: 3489 3118 435 BsrBI CCG'CTC Cuts at: 1394 1823 3432 4929 1394 Size: 429 1609 1497 3507 Fragments arranged by size: 3507 1609 1497 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3747 3865 5537 5711 65 Size: 1183 2499 118 1672 174 1396 Fragments arranged by size: 2499 1672 1396 1183 174 118 BsrFI r'CCGG_y Cuts at: 1700 1810 2078 3182 5686 5873 1700 Size: 110 268 1104 2504 187 2869 Fragments arranged by size: 2869 2504 1104 268 187 110 BsrGI T'GTAC_A Cuts at: 96 3279 3474 3724 96 Size: 3183 195 250 3414 Fragments arranged by size: 3414 3183 250 195 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7042 BssSI C'ACGA_G Cuts at: 5102 6114 5102 Size: 1012 6030 BstAPI GCAn_nnn'nTGC Cuts at: 794 2178 794 Size: 1384 5658 BstBI TT'CG_AA Cuts at: 2007 5846 2007 Size: 3839 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7042 BstXI CCAn_nnnn'nTGG Cuts at: 2094 2704 3718 2094 Size: 610 1014 5418 Fragments arranged by size: 5418 1014 610 BstYI r'GATC_y Cuts at: 1972 3243 4179 4372 4739 5124 5141 6593 6679 Size: 1271 936 193 367 385 17 1452 86 Cuts at: 6679 6691 7037 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1271 936 385 367 346 193 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2893 2981 3093 513 Size: 552 1828 88 112 4462 Fragments arranged by size: 4462 1828 552 112 88 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6303 Fragments arranged by size: 6303 683 56 BtsI GCAGTG_nn' Cuts at: 2424 4137 4558 5352 5372 2424 Size: 1713 421 794 20 4094 Fragments arranged by size: 4094 1713 794 421 20 ClaI AT'CG_AT Cuts at: 1554 4324 1554 Size: 2770 4272 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2118 2153 529 Size: 35 1554 35 5418 Fragments arranged by size: 5418 1554 35 35 DraI TTT'AAA Cuts at: 2748 4346 2748 Size: 1598 5444 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6049 809 Size: 507 4733 1802 Fragments arranged by size: 4733 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2035 2106 2325 Size: 54 1425 50 275 18 205 71 219 Cuts at: 2325 2955 3090 3433 3652 4158 4489 5378 7007 Size: 630 135 343 219 506 331 889 1629 Cuts at: 7007 8 Size: 43 Fragments arranged by size: 1629 1425 889 630 506 343 331 275 219 219 205 135 71 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4489 1537 Size: 275 2677 4090 Fragments arranged by size: 4090 2677 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2655 3401 4972 1353 Size: 1302 746 1571 3423 Fragments arranged by size: 3423 1571 1302 746 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5629 6003 6149 387 Size: 1064 138 4040 374 146 1280 Fragments arranged by size: 4040 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2734 2734 Size: 7042 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2318 2367 2435 2582 2636 2720 2798 Size: 393 686 49 68 147 54 84 78 Cuts at: 2798 2822 3047 3554 4254 5105 5702 6489 6757 Size: 24 225 507 700 851 597 787 268 Cuts at: 6757 1239 Size: 1524 Fragments arranged by size: 1524 851 787 700 686 597 507 393 268 225 147 84 78 68 54 49 24 EcoO109I rG'GnC_Cy Cuts at: 2884 2911 3833 3974 2884 Size: 27 922 141 5952 Fragments arranged by size: 5952 922 141 27 EcoRI G'AATT_C Cuts at: 4064 4351 4729 4064 Size: 287 378 6377 Fragments arranged by size: 6377 378 287 FspI TGC'GCA Cuts at: 2756 5548 2756 Size: 2792 4250 HaeII r_GCGC'y Cuts at: 1678 6189 1678 Size: 4511 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5683 5715 5757 5789 1373 Size: 32 135 32 4111 32 42 32 2626 Fragments arranged by size: 4111 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2620 2682 2788 4386 4632 669 Size: 988 281 682 62 106 1598 246 3079 Fragments arranged by size: 3079 1598 988 682 281 246 106 62 HindIII A'AGCT_T Cuts at: 748 748 Size: 7042 HpaI GTT'AAC Cuts at: 2682 4632 2682 Size: 1950 5092 KpnI G_GTAC'C Cuts at: 3537 4367 3537 Size: 830 6212 MfeI C'AATT_G Cuts at: 4641 4641 Size: 7042 MluI A'CGCG_T Cuts at: 6918 6918 Size: 7042 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4142 6156 6340 1305 Size: 2837 2014 184 2007 Fragments arranged by size: 2837 2014 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2108 3092 3654 10 Size: 54 1425 343 276 984 562 3398 Fragments arranged by size: 3398 1425 984 562 343 276 54 MslI CAynn'nnrTG Cuts at: 518 1166 2031 2092 2429 3716 3732 4154 5000 Size: 648 865 61 337 1287 16 422 846 Cuts at: 5000 5359 5518 518 Size: 359 159 2042 Fragments arranged by size: 2042 1287 865 846 648 422 359 337 159 61 16 MspA1I CmG'CkG Cuts at: 1606 2608 2824 2983 2998 3377 3700 3706 3989 Size: 1002 216 159 15 379 323 6 283 Cuts at: 3989 4458 5135 6283 6528 1606 Size: 469 677 1148 245 2120 Fragments arranged by size: 2120 1148 1002 677 469 379 323 283 245 216 159 15 6 NaeI GCC'GGC Cuts at: 2080 2080 Size: 7042 NcoI C'CATG_G Cuts at: 513 1065 2893 513 Size: 552 1828 4662 Fragments arranged by size: 4662 1828 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7042 NgoMIV G'CCGG_C Cuts at: 2078 2078 Size: 7042 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7042 NotI GC'GGCC_GC Cuts at: 4489 4489 Size: 7042 NspI r_CATG'y Cuts at: 1132 1229 3475 4400 5945 1132 Size: 97 2246 925 1545 2229 Fragments arranged by size: 2246 2229 1545 925 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7042 PciI A'CATG_T Cuts at: 5941 5941 Size: 7042 PflMI CCAn_nnn'nTGG Cuts at: 1108 4244 4258 1108 Size: 3136 14 3892 Fragments arranged by size: 3892 3136 14 PfoI T'CCnGG_A Cuts at: 2412 3246 2412 Size: 834 6208 PmeI GTTT'AAAC Cuts at: 4346 4346 Size: 7042 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5111 5143 6624 6656 5111 Size: 32 1481 32 5497 Fragments arranged by size: 5497 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2911 3833 3974 2911 Size: 922 141 5979 Fragments arranged by size: 5979 922 141 PshAI GACnn'nnGTC Cuts at: 1613 2909 1613 Size: 1296 5746 PsiI TTA'TAA Cuts at: 4612 4612 Size: 7042 PstI C_TGCA'G Cuts at: 830 2247 2376 2867 3705 4394 830 Size: 1417 129 491 838 689 3478 Fragments arranged by size: 3478 1417 838 689 491 129 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7042 PvuII CAG'CTG Cuts at: 2608 2824 2998 3700 3989 2608 Size: 216 174 702 289 5661 Fragments arranged by size: 5661 702 289 216 174 SacI G_AGCT'C Cuts at: 2736 2736 Size: 7042 SacII CC_GC'GG Cuts at: 2984 2984 Size: 7042 SalI G'TCGA_C Cuts at: 1936 4384 1936 Size: 2448 4594 SanDI GG'GwC_CC Cuts at: 3833 3833 Size: 7042 SapI GCTCTTCn'nnn_ Cuts at: 2655 2655 Size: 7042 SbfI CC_TGCA'GG Cuts at: 4394 4394 Size: 7042 ScaI AGT'ACT Cuts at: 5290 7002 5290 Size: 1712 5330 SexAI A'CCwGG_T Cuts at: 3932 3932 Size: 7042 SfcI C'TryA_G Cuts at: 826 1045 2243 2372 2863 3701 4390 5525 6206 Size: 219 1198 129 491 838 689 1135 681 Cuts at: 6206 6397 826 Size: 191 1471 Fragments arranged by size: 1471 1198 1135 838 689 681 491 219 191 129 SfiI GGCCn_nnn'nGGCC Cuts at: 4254 4254 Size: 7042 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7042 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7042 SmaI CCC'GGG Cuts at: 1872 3426 4369 1872 Size: 1554 943 4545 Fragments arranged by size: 4545 1554 943 SmlI C'TyrA_G Cuts at: 820 1017 1943 2564 5146 6047 6309 820 Size: 197 926 621 2582 901 262 1553 Fragments arranged by size: 2582 1553 926 901 621 262 197 SnaBI TAC'GTA Cuts at: 493 4339 4833 493 Size: 3846 494 2702 Fragments arranged by size: 3846 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7042 SphI G_CATG'C Cuts at: 1132 4400 1132 Size: 3268 3774 SspI AAT'ATT Cuts at: 5 52 4966 5 Size: 47 4914 2081 Fragments arranged by size: 4914 2081 47 StuI AGG'CCT Cuts at: 1517 2591 4257 1517 Size: 1074 1666 4302 Fragments arranged by size: 4302 1666 1074 StyI C'CwwG_G Cuts at: 513 1065 2202 2893 3153 4252 4481 513 Size: 552 1137 691 260 1099 229 3074 Fragments arranged by size: 3074 1137 1099 691 552 260 229 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2921 5069 5086 5239 5424 5869 325 Size: 1255 1341 2148 17 153 185 445 1498 Fragments arranged by size: 2148 1498 1341 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3279 3474 3724 Size: 274 80 33 51 1443 1302 195 250 Cuts at: 3724 5288 7000 96 Size: 1564 1712 138 Fragments arranged by size: 1712 1564 1443 1302 274 250 195 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2095 2127 3834 3866 7002 7034 2095 Size: 32 1707 32 3136 32 2103 Fragments arranged by size: 3136 2103 1707 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7042 XbaI T'CTAG_A Cuts at: 4378 4378 Size: 7042 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4190 4190 Size: 7042 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7042 XmaI C'CCGG_G Cuts at: 1870 3424 4367 1870 Size: 1554 943 4545 Fragments arranged by size: 4545 1554 943 XmnI GAAnn'nnTTC Cuts at: 2342 2670 5171 2342 Size: 328 2501 4213 Fragments arranged by size: 4213 2501 328 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4850 276 Size: 53 83 186 936 3316 2468 Fragments arranged by size: 3316 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SfoI SrfI SwaI