Restriction map for
pFN21ASDA0517
(Linear) (Six-base) MAPSORT of: KIAA0517.seq Check: 8180 from: 1 to: 2313
KIAA0517.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
Acc65I G'GTAC_C
Cuts at: 0 1510 2313
Size: 1510 803
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1024 1531 2231 2313
Size: 613 411 507 700 82
Fragments arranged by size:
700 613 507 411 82
AleI CACnn'nnGTG
Cuts at: 0 8 406 1693 2313
Size: 8 398 1287 620
Fragments arranged by size:
1287 620 398 8
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 53 757 2105 2313
Size: 53 704 1348 208
Fragments arranged by size:
1348 704 208 53
AlwNI CAG_nnn'CTG
Cuts at: 0 141 403 2275 2313
Size: 141 262 1872 38
Fragments arranged by size:
1872 262 141 38
ApaLI G'TGCA_C
Cuts at: 0 501 2313
Size: 501 1812
ApoI r'AATT_y
Cuts at: 0 460 1774 2041 2313
Size: 460 1314 267 272
Fragments arranged by size:
1314 460 272 267
AseI AT'TA_AT
Cuts at: 0 1582 2313
Size: 1582 731
AvaI C'yCGr_G
Cuts at: 0 289 1024 1401 2313
Size: 289 735 377 912
Fragments arranged by size:
912 735 377 289
BamHI G'GATC_C
Cuts at: 0 2156 2313
Size: 2156 157
BanI G'GyrC_C
Cuts at: 0 94 215 1240 1510 2313
Size: 94 121 1025 270 803
Fragments arranged by size:
1025 803 270 121 94
BanII G_rGCy'C
Cuts at: 0 713 2313
Size: 713 1600
BbsI GAAGACnn'nnnn_
Cuts at: 0 374 724 1493 2313
Size: 374 350 769 820
Fragments arranged by size:
820 769 374 350
BbvCI CC'TCA_GC
Cuts at: 0 374 2313
Size: 374 1939
BciVI GTATCCnnnnn_n'
Cuts at: 0 798 2313
Size: 798 1515
BglI GCCn_nnn'nGGC
Cuts at: 0 2231 2313
Size: 2231 82
Bme1580I G_kGCm'C
Cuts at: 0 505 1844 2313
Size: 505 1339 469
Fragments arranged by size:
1339 505 469
BmgBI CAC'GTC
Cuts at: 0 345 1416 2313
Size: 345 1071 897
Fragments arranged by size:
1071 897 345
BmrI ACTGGGnnnn_n'
Cuts at: 0 256 1703 2151 2313
Size: 256 1447 448 162
Fragments arranged by size:
1447 448 256 162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 344 412 559 697 775 799 2313
Size: 295 49 68 147 138 78 24 1514
Fragments arranged by size:
1514 295 147 138 78 68 49 24
Bpu10I CC'TnA_GC
Cuts at: 0 374 2313
Size: 374 1939
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 2313
Size: 526 1787
BsaI GGTCTCn'nnnn_
Cuts at: 0 874 2313
Size: 874 1439
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 30 1685 1715 1811 1841 2313
Size: 30 1655 30 96 30 472
Fragments arranged by size:
1655 472 96 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 391 1752 2313
Size: 391 1361 561
Fragments arranged by size:
1361 561 391
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 181 1630 2313
Size: 59 122 1449 683
Fragments arranged by size:
1449 683 122 59
BsiHKAI G_wGCw'C
Cuts at: 0 505 518 527 713 1192 2313
Size: 505 13 9 186 479 1121
Fragments arranged by size:
1121 505 479 186 13 9
Bsp1286I G_dGCh'C
Cuts at: 0 505 518 527 713 1192 1844 2313
Size: 505 13 9 186 479 652 469
Fragments arranged by size:
652 505 479 469 186 13 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1939 2313
Size: 1939 374
BsrBI CCG'CTC
Cuts at: 0 1409 2313
Size: 1409 904
BsrDI GCAATG_nn'
Cuts at: 0 1724 1842 2313
Size: 1724 118 471
Fragments arranged by size:
1724 471 118
BsrFI r'CCGG_y
Cuts at: 0 55 1159 2313
Size: 55 1104 1154
Fragments arranged by size:
1154 1104 55
BsrGI T'GTAC_A
Cuts at: 0 1256 1451 1701 2313
Size: 1256 195 250 612
Fragments arranged by size:
1256 612 250 195
BstAPI GCAn_nnn'nTGC
Cuts at: 0 155 2313
Size: 155 2158
BstXI CCAn_nnnn'nTGG
Cuts at: 0 71 681 1695 2313
Size: 71 610 1014 618
Fragments arranged by size:
1014 618 610 71
BstYI r'GATC_y
Cuts at: 0 1220 2156 2313
Size: 1220 936 157
Fragments arranged by size:
1220 936 157
BtgI C'CryG_G
Cuts at: 0 870 958 1070 2313
Size: 870 88 112 1243
Fragments arranged by size:
1243 870 112 88
BtsI GCAGTG_nn'
Cuts at: 0 401 2114 2313
Size: 401 1713 199
Fragments arranged by size:
1713 401 199
ClaI AT'CG_AT
Cuts at: 0 2301 2313
Size: 2301 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 95 130 2313
Size: 95 35 2183
Fragments arranged by size:
2183 95 35
DraI TTT'AAA
Cuts at: 0 725 2313
Size: 725 1588
EaeI y'GGCC_r
Cuts at: 0 12 83 302 932 1067 1410 1629 2135
Size: 12 71 219 630 135 343 219 506
Cuts at: 2135 2313
Size: 178
Fragments arranged by size:
630 506 343 219 219 178 135 71
12
EarI CTCTTCn'nnn_
Cuts at: 0 632 1378 2313
Size: 632 746 935
Fragments arranged by size:
935 746 632
EcoICRI GAG'CTC
Cuts at: 0 711 2313
Size: 711 1602
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 344 412 559 613 697 775 799
Size: 295 49 68 147 54 84 78 24
Cuts at: 799 1024 1531 2231 2313
Size: 225 507 700 82
Fragments arranged by size:
700 507 295 225 147 84 82 78
68 54 49 24
EcoO109I rG'GnC_Cy
Cuts at: 0 861 888 1810 1951 2313
Size: 861 27 922 141 362
Fragments arranged by size:
922 861 362 141 27
EcoRI G'AATT_C
Cuts at: 0 2041 2313
Size: 2041 272
FspI TGC'GCA
Cuts at: 0 733 2313
Size: 733 1580
HincII GTy'rAC
Cuts at: 0 597 659 765 2313
Size: 597 62 106 1548
Fragments arranged by size:
1548 597 106 62
HpaI GTT'AAC
Cuts at: 0 659 2313
Size: 659 1654
KpnI G_GTAC'C
Cuts at: 0 1514 2313
Size: 1514 799
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2119 2313
Size: 2119 194
MscI TGG'CCA
Cuts at: 0 85 1069 1631 2313
Size: 85 984 562 682
Fragments arranged by size:
984 682 562 85
MslI CAynn'nnrTG
Cuts at: 0 8 69 406 1693 1709 2131 2313
Size: 8 61 337 1287 16 422 182
Fragments arranged by size:
1287 422 337 182 61 16 8
MspA1I CmG'CkG
Cuts at: 0 585 801 960 975 1354 1677 1683 1966
Size: 585 216 159 15 379 323 6 283
Cuts at: 1966 2313
Size: 347
Fragments arranged by size:
585 379 347 323 283 216 159 15
6
NaeI GCC'GGC
Cuts at: 0 57 2313
Size: 57 2256
NcoI C'CATG_G
Cuts at: 0 870 2313
Size: 870 1443
NgoMIV G'CCGG_C
Cuts at: 0 55 2313
Size: 55 2258
NspI r_CATG'y
Cuts at: 0 1452 2313
Size: 1452 861
PflMI CCAn_nnn'nTGG
Cuts at: 0 2221 2235 2313
Size: 2221 14 78
Fragments arranged by size:
2221 78 14
PfoI T'CCnGG_A
Cuts at: 0 389 1223 2313
Size: 389 834 1090
Fragments arranged by size:
1090 834 389
PpuMI rG'GwC_Cy
Cuts at: 0 888 1810 1951 2313
Size: 888 922 141 362
Fragments arranged by size:
922 888 362 141
PshAI GACnn'nnGTC
Cuts at: 0 886 2313
Size: 886 1427
PstI C_TGCA'G
Cuts at: 0 224 353 844 1682 2313
Size: 224 129 491 838 631
Fragments arranged by size:
838 631 491 224 129
PvuII CAG'CTG
Cuts at: 0 585 801 975 1677 1966 2313
Size: 585 216 174 702 289 347
Fragments arranged by size:
702 585 347 289 216 174
SacI G_AGCT'C
Cuts at: 0 713 2313
Size: 713 1600
SacII CC_GC'GG
Cuts at: 0 961 2313
Size: 961 1352
SanDI GG'GwC_CC
Cuts at: 0 1810 2313
Size: 1810 503
SapI GCTCTTCn'nnn_
Cuts at: 0 632 2313
Size: 632 1681
SexAI A'CCwGG_T
Cuts at: 0 1909 2313
Size: 1909 404
SfcI C'TryA_G
Cuts at: 0 220 349 840 1678 2313
Size: 220 129 491 838 635
Fragments arranged by size:
838 635 491 220 129
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2231 2313
Size: 2231 82
SmaI CCC'GGG
Cuts at: 0 1403 2313
Size: 1403 910
SmlI C'TyrA_G
Cuts at: 0 541 2313
Size: 541 1772
StuI AGG'CCT
Cuts at: 0 568 2234 2313
Size: 568 1666 79
Fragments arranged by size:
1666 568 79
StyI C'CwwG_G
Cuts at: 0 179 870 1130 2229 2313
Size: 179 691 260 1099 84
Fragments arranged by size:
1099 691 260 179 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 898 2313
Size: 898 1415
TatI w'GTAC_w
Cuts at: 0 1256 1451 1701 2313
Size: 1256 195 250 612
Fragments arranged by size:
1256 612 250 195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 72 104 1811 1843 2313
Size: 72 32 1707 32 470
Fragments arranged by size:
1707 470 72 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2167 2313
Size: 2167 146
XmaI C'CCGG_G
Cuts at: 0 1401 2313
Size: 1401 912
XmnI GAAnn'nnTTC
Cuts at: 0 319 647 2313
Size: 319 328 1666
Fragments arranged by size:
1666 328 319
Enzymes that do cut:
Acc65I AcuI AleI AlfI AlwNI ApaLI ApoI AseI
AvaI BamHI BanI BanII BbsI BbvCI BciVI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaXI
BseRI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstXI BstYI BtgI BtsI ClaI CspCI
DraI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FspI
HincII HpaI KpnI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PflMI PfoI PpuMI PshAI PstI
PvuII SacI SacII SanDI SapI SexAI SfcI SfiI
SmaI SmlI StuI StyI TaqII TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI AscI AsiSI AvrII BaeI BbeI
BcgI BclI BfrBI BglII BlpI BmtI BplI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI DraIII DrdI EagI EciI EcoNI EcoRV FalI
FseI FspAI HaeII Hin4I HindIII KasI MfeI MluI
NarI NdeI NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI
PvuI RsrII SalI SbfI ScaI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XhoI ZraI