Restriction map for
pFN21ASDA0513
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BlnI
BlpI Bpu1102I BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspLU11I BspMI BsrFI BsrI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI MaeI MamI MfeI
MluI MroI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI TfiI Tru9I
Tsp509I Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 255 GTCTAC GT^[AC][GT]AC
AlwI 339 GGATC GGATC(4/5)
AlwNI 583 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 46 CCTGAGG CC^T[ACGT]AGG
Aor51HI 428 AGCGCT AGC^GCT
ApaI 760 GGGCCC GGGCC^C
Asp700I 312 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 246 GAGCAC G[AT]GC[AT]^C
AspI 937 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 784 GAAGAC GAAGAC(2/6)
BglI 894 GCCCATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 784 GAAGAC GAAGAC(2/6)
BsaWI 1055 TCCGGT [AT]^CCGG[AT]
BsgI 475 GTGCAG GTGCAG(16/14)
BsiHKAI 246 GAGCAC G[AT]GC[AT]^C
BsmI 921 GAATGC GAATGC(1/-1)
Bsp1407I 872 TGTACA T^GTACA
BspHI 542 TCATGA T^CATGA
BsrBI 682 GAGCGG CCGCTC(-3/-3)
BsrDI 1139 GCAATG GCAATG(2/0)
BsrGI 872 TGTACA T^GTACA
BstAPI 1101 GCACAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 46 CCTGAGG CC^T[ACGT]AGG
DrdI 138 GACCACTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 428 AGCGCT AGC^GCT
Eco81I 46 CCTGAGG CC^T[ACGT]AGG
EcoNI 701 CCTCGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 428 AGCGCT [GA]GCGC^[CT]
HgaI 937 GACGC GACGC(5/10)
KspI 614 CCGCGG CCGC^GG
MslI 543 CATGACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 1106 ACATGC [GA]CATG^[CT]
PflFI 937 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 14 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 542 TCATGA T^CATGA
SacII 614 CCGCGG CCGC^GG
SapI 1183 GAAGAGC GCTCTTC(1/4)
ScaI 368 AGTACT AGT^ACT
SmaI 602 CCCGGG CCC^GGG
SspBI 872 TGTACA T^GTACA
Tth111I 937 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 602 CCCGGG C^CCGGG
XmaI 602 CCCGGG C^CCGGG
XmnI 312 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AvaI 602 CCCGGG C^[CT]CG[GA]G
838 CCCGAG
AvaII 15 GGTCC G^G[AT]CC
198 GGACC
BalI 767 TGGCCA TGG^CCA
1223 TGGCCA
BanII 24 GGGCTC G[GA]GC[CT]^C
760 GGGCCC
BglII 314 AGATCT A^GATCT
863 AGATCT
BsaAI 304 TACGTG [CT]AC^GT[GA]
415 TACGTG
BsoBI 602 CCCGGG C^[CT]CG[GA]G
838 CCCGAG
BstYI 314 AGATCT [GA]^GATC[CT]
863 AGATCT
CfoI 429 GCGC GCG^C
1041 GCGC
DsaI 20 CCGTGG C^C[GA][CT]GG
614 CCGCGG
FauI 613 CCCGC CCCGC(4/6)
616 GCGGG
HhaI 429 GCGC GCG^C
1041 GCGC
HinP1I 429 GCGC G^CGC
1041 GCGC
HphI 557 TCACC GGTGA(8/7)
1073 TCACC
MaeII 305 ACGT A^CGT
416 ACGT
MaeIII 823 GTGAC ^GT[ACGT]AC
1205 GTGAC
MflI 314 AGATCT [GA]^GATC[CT]
863 AGATCT
MluNI 767 TGGCCA TGG^CCA
1223 TGGCCA
MscI 767 TGGCCA TGG^CCA
1223 TGGCCA
PstI 91 CTGCAG CTGCA^G
372 CTGCAG
SfcI 91 CTGCAG C^T[GA][CT]AG
372 CTGCAG
Tsp45I 823 GTGAC ^GT[GC]AC
1205 GTGAC
XhoII 314 AGATCT [GA]^GATC[CT]
863 AGATCT
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AfaI AluI BanI
BbvI BcnI BmyI BpmI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI Ksp632I MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI TspRI
TthHB8I