(Circular) (Six-base) MAPSORT of: pFN21ASDA0513.seq Check: 9475 from: 1 to: 5962 pFN21ASDA0513 5962 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3772 278 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2279 3305 1937 Size: 342 1026 4594 Fragments arranged by size: 4594 1026 342 Acc65I G'GTAC_C Cuts at: 3283 3283 Size: 5962 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2735 2798 2927 3194 4025 5409 5677 1632 Size: 1103 63 129 267 831 1384 268 1917 Fragments arranged by size: 1917 1384 1103 831 268 267 129 63 AfeI AGC'GCT Cuts at: 2453 2453 Size: 5962 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 4861 5838 1203 Size: 3658 977 1327 Fragments arranged by size: 3658 1327 977 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 4691 4691 Size: 5962 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5962 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2523 1184 Size: 1339 4623 AlwNI CAG_nnn'CTG Cuts at: 2611 3705 5277 2611 Size: 1094 1572 3296 Fragments arranged by size: 3296 1572 1094 ApaI G_GGCC'C Cuts at: 2787 2787 Size: 5962 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 3271 3497 3649 5758 1493 Size: 1778 226 152 2109 1697 Fragments arranged by size: 2109 1778 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 2625 2861 3287 1870 Size: 73 682 236 426 4545 Fragments arranged by size: 4545 682 426 236 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2428 2461 2428 Size: 33 5929 BamHI G'GATC_C Cuts at: 3292 3659 3292 Size: 367 5595 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2074 2098 3042 3283 4738 Size: 325 217 627 287 24 944 241 1455 Cuts at: 4738 618 Size: 1842 Fragments arranged by size: 1842 1455 944 627 325 287 241 217 24 BanII G_rGCy'C Cuts at: 1391 2051 2787 3281 1391 Size: 660 736 494 4072 Fragments arranged by size: 4072 736 660 494 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2814 928 Size: 971 915 4076 Fragments arranged by size: 4076 971 915 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4153 4187 1903 Size: 34 2216 34 3678 Fragments arranged by size: 3678 2216 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3854 5064 3854 Size: 1210 4752 BclI T'GATC_A Cuts at: 1548 1548 Size: 5962 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2923 4573 136 Size: 107 122 71 631 605 1251 1650 1525 Fragments arranged by size: 1650 1525 1251 631 605 122 107 71 BglII A'GATC_T Cuts at: 2337 2886 5957 2337 Size: 549 3071 2342 Fragments arranged by size: 3071 2342 549 BlpI GC'TnA_GC Cuts at: 3379 3379 Size: 5962 Bme1580I G_kGCm'C Cuts at: 1790 2449 2787 3116 4023 5179 1790 Size: 659 338 329 907 1156 2573 Fragments arranged by size: 2573 1156 907 659 338 329 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5962 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3693 4651 442 Size: 416 53 83 399 24 2276 958 1753 Fragments arranged by size: 2276 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5962 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2221 2272 2221 Size: 51 5911 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 2367 2786 3033 4622 1239 Size: 875 253 419 247 1589 2579 Fragments arranged by size: 2579 1589 875 419 253 247 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4087 4952 5250 4087 Size: 865 298 4799 Fragments arranged by size: 4799 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2021 2153 2288 2762 4625 882 Size: 617 522 132 135 474 1863 2219 Fragments arranged by size: 2219 1863 617 522 474 135 132 BsaAI yAC'GTr Cuts at: 493 1204 2329 2440 3259 3753 493 Size: 711 1125 111 819 494 2702 Fragments arranged by size: 2702 1125 819 711 494 111 BsaBI GATnn'nnATC Cuts at: 5956 5956 Size: 5962 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3769 4151 4786 275 Size: 53 83 186 936 2236 382 635 1451 Fragments arranged by size: 2236 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3078 4395 4793 5067 5214 5625 3078 Size: 1317 398 274 147 411 3415 Fragments arranged by size: 3415 1317 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2071 2101 3026 3056 1178 Size: 30 165 30 668 30 925 30 4084 Fragments arranged by size: 4084 925 668 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2230 2248 2384 3032 3035 3038 3074 1186 Size: 1044 18 136 648 3 3 36 4074 Fragments arranged by size: 4074 1044 648 136 36 18 3 3 BseYI C'CCAG_C Cuts at: 2448 3109 5165 5661 2448 Size: 661 2056 496 2749 Fragments arranged by size: 2749 2056 661 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2519 1163 Size: 573 783 4606 Fragments arranged by size: 4606 783 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3412 4173 5201 1540 Size: 275 186 1411 761 1028 2301 Fragments arranged by size: 2301 1411 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2273 4023 4108 5179 2273 Size: 1750 85 1071 3056 Fragments arranged by size: 3056 1750 1071 85 BsmI GAATG_Cn' Cuts at: 2950 3473 3566 2950 Size: 523 93 5346 Fragments arranged by size: 5346 523 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2273 2449 2787 3116 3281 4023 Size: 399 261 222 176 338 329 165 742 Cuts at: 4023 4108 5179 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 338 329 261 222 176 165 85 BspEI T'CCGG_A Cuts at: 5625 5625 Size: 5962 BspHI T'CATG_A Cuts at: 2565 3851 5581 2565 Size: 1286 1730 2946 Fragments arranged by size: 2946 1730 1286 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3317 844 Size: 2473 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2707 3849 1394 Size: 429 884 1142 3507 Fragments arranged by size: 3507 1142 884 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3169 4457 4631 65 Size: 1183 1921 1288 174 1396 Fragments arranged by size: 1921 1396 1288 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4606 4793 1700 Size: 110 2796 187 2869 Fragments arranged by size: 2869 2796 187 110 BsrGI T'GTAC_A Cuts at: 96 2895 96 Size: 2799 3163 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 4022 5034 4022 Size: 1012 4950 BstAPI GCAn_nnn'nTGC Cuts at: 794 3130 794 Size: 2336 3626 BstBI TT'CG_AA Cuts at: 2007 4766 2007 Size: 2759 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5962 BstYI r'GATC_y Cuts at: 1972 2337 2886 3292 3659 4044 4061 5513 5599 Size: 365 549 406 367 385 17 1452 86 Cuts at: 5599 5611 5957 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 549 406 385 367 365 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2070 2070 Size: 5962 BtgI C'CryG_G Cuts at: 513 1065 2022 2043 2637 513 Size: 552 957 21 594 3838 Fragments arranged by size: 3838 957 594 552 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2139 2683 487 Size: 683 56 913 544 3766 Fragments arranged by size: 3766 913 683 544 56 BtsI GCAGTG_nn' Cuts at: 2407 2517 3478 4272 4292 2407 Size: 110 961 794 20 4077 Fragments arranged by size: 4077 961 794 110 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5962 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5927 DraI TTT'AAA Cuts at: 3266 3266 Size: 5962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2167 4969 809 Size: 507 851 2802 1802 Fragments arranged by size: 2802 1802 851 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2682 2727 2790 Size: 54 1425 50 275 18 852 45 63 Cuts at: 2790 3246 3409 4298 5927 8 Size: 456 163 889 1629 43 Fragments arranged by size: 1629 1425 889 852 456 275 163 63 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3409 1537 Size: 275 1597 4090 Fragments arranged by size: 4090 1597 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2089 2286 3023 3201 3892 1353 Size: 736 197 737 178 691 3423 Fragments arranged by size: 3423 737 736 691 197 178 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4549 4923 5069 387 Size: 1064 138 2960 374 146 1280 Fragments arranged by size: 2960 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2367 2735 2786 2798 2927 3033 Size: 393 482 253 368 51 12 129 106 Cuts at: 3033 3194 4025 4622 5409 5677 1239 Size: 161 831 597 787 268 1524 Fragments arranged by size: 1524 831 787 597 482 393 368 268 253 161 129 106 51 12 EcoNI CCTnn'n_nnAGG Cuts at: 2728 2728 Size: 5962 EcoO109I rG'GnC_Cy Cuts at: 2038 2783 2784 2038 Size: 745 1 5216 Fragments arranged by size: 5216 745 1 EcoRI G'AATT_C Cuts at: 3271 3649 3271 Size: 378 5584 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2770 2770 Size: 5962 FspI TGC'GCA Cuts at: 4468 4468 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 2455 5109 1678 Size: 777 2654 2531 Fragments arranged by size: 2654 2531 777 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2132 2164 4603 4635 4677 Size: 32 135 32 560 32 2439 32 42 Cuts at: 4677 4709 1373 Size: 32 2626 Fragments arranged by size: 2626 2439 560 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3306 3552 669 Size: 988 281 1368 246 3079 Fragments arranged by size: 3079 1368 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5962 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KpnI G_GTAC'C Cuts at: 3287 3287 Size: 5962 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2268 2286 5076 5260 1305 Size: 963 18 2790 184 2007 Fragments arranged by size: 2790 2007 963 184 18 MscI TGG'CCA Cuts at: 10 64 1489 1832 2792 3248 10 Size: 54 1425 343 960 456 2724 Fragments arranged by size: 2724 1425 960 456 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2570 3920 4279 4438 518 Size: 648 1404 1350 359 159 2042 Fragments arranged by size: 2042 1404 1350 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2608 2639 2676 3109 3378 4055 5203 5448 Size: 1002 31 37 433 269 677 1148 245 Cuts at: 5448 1606 Size: 2120 Fragments arranged by size: 2120 1148 1002 677 433 269 245 37 31 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4453 Fragments arranged by size: 4453 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5962 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 3133 3320 4865 1132 Size: 97 1904 187 1545 2229 Fragments arranged by size: 2229 1904 1545 187 97 PasI CC'CwG_GG Cuts at: 1348 2134 2838 1348 Size: 786 704 4472 Fragments arranged by size: 4472 786 704 PciI A'CATG_T Cuts at: 4861 4861 Size: 5962 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5962 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4031 4063 5544 5576 4031 Size: 32 1481 32 4417 Fragments arranged by size: 4417 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2038 2038 Size: 5962 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5962 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PspOMI G'GGCC_C Cuts at: 2783 2783 Size: 5962 PstI C_TGCA'G Cuts at: 830 2118 2399 3314 830 Size: 1288 281 915 3478 Fragments arranged by size: 3478 1288 915 281 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 PvuII CAG'CTG Cuts at: 2608 2676 3109 2608 Size: 68 433 5461 Fragments arranged by size: 5461 433 68 SacII CC_GC'GG Cuts at: 2640 2640 Size: 5962 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SapI GCTCTTCn'nnn_ Cuts at: 3201 3201 Size: 5962 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 2393 4210 5922 2393 Size: 1817 1712 2433 Fragments arranged by size: 2433 1817 1712 SfcI C'TryA_G Cuts at: 826 1045 2114 2395 3310 4445 5126 5317 826 Size: 219 1069 281 915 1135 681 191 1471 Fragments arranged by size: 1471 1135 1069 915 681 281 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 2627 3289 1872 Size: 755 662 4545 Fragments arranged by size: 4545 755 662 SmlI C'TyrA_G Cuts at: 820 1017 1943 4066 4967 5229 820 Size: 197 926 2123 901 262 1553 Fragments arranged by size: 2123 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3259 3753 493 Size: 2766 494 2702 Fragments arranged by size: 2766 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 3320 1132 Size: 2188 3774 SspI AAT'ATT Cuts at: 5 52 3886 5 Size: 47 3834 2081 Fragments arranged by size: 3834 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5962 StyI C'CwwG_G Cuts at: 513 1065 2022 3401 513 Size: 552 957 1379 3074 Fragments arranged by size: 3074 1379 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2092 3989 4006 4159 4344 4789 325 Size: 1255 512 1897 17 153 185 445 1498 Fragments arranged by size: 1897 1498 1255 512 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2391 2895 4208 Size: 274 80 33 51 1443 414 504 1313 Cuts at: 4208 5920 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1313 504 414 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2071 2103 3026 3058 5922 5954 2071 Size: 32 923 32 2864 32 2079 Fragments arranged by size: 2864 2079 923 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2963 1316 Size: 1647 4315 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 2625 3287 1870 Size: 755 662 4545 Fragments arranged by size: 4545 755 662 XmnI GAAnn'nnTTC Cuts at: 2339 4091 2339 Size: 1752 4210 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3770 276 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI