Restriction map for
pFN21ASDA0482
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
ApoI AscI AseI AsnI Asp700I
AspI AviII AvrII BbrPI BclI
BfrI BlnI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DraI DraIII EagI
EclXI Eco52I EcoRI EcoT22I FbaI
FseI FspI HincII HindII HpaI
KspI MfeI MluI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp509I Tth111I XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AccI 694 GTCTAC GT^[AC][GT]AC
AccIII 3563 TCCGGA T^CCGGA
AhdI 349 GACAACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 349 GACAACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1537 GGCGCC GGCGC^C
BglII 1643 AGATCT A^GATCT
BsaAI 3370 TACGTG [CT]AC^GT[GA]
BsaBI 1654 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3563 TCCGGA [AT]^CCGG[AT]
BseAI 3563 TCCGGA T^CCGGA
BspEI 3563 TCCGGA T^CCGGA
BspLU11I 587 ACATGT A^CATGT
DrdI 3412 GACCAGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 349 GACAACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1453 GATATC GAT^ATC
HindIII 436 AAGCTT A^AGCTT
KasI 1537 GGCGCC G^GCGCC
MamI 1654 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3563 TCCGGA T^CCGGA
MseI 3365 TTAA T^TAA
NarI 1537 GGCGCC GG^CGCC
NspI 587 ACATGT [GA]CATG^[CT]
SfoI 1537 GGCGCC GGC^GCC
SmaI 389 CCCGGG CCC^GGG
Tru9I 3365 TTAA T^TAA
XbaI 184 TCTAGA T^CTAGA
XmaCI 389 CCCGGG C^CCGGG
XmaI 389 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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Alw44I 1333 GTGCAC G^TGCAC
3550 GTGCAC
Aor51HI 2052 AGCGCT AGC^GCT
2519 AGCGCT
ApaLI 1333 GTGCAC G^TGCAC
3550 GTGCAC
BamHI 921 GGATCC G^GATCC
3381 GGATCC
BbsI 793 GTCTTC GAAGAC(2/6)
889 GTCTTC
BcgI 2494 CGACACCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3504 GCAGCAGCCTCG
BciVI 651 GGATAC GTATCC(6/5)
1055 GGATAC
BlpI 3052 GCTGAGC GC^T[ACGT]AGC
3343 GCTGAGC
Bpu1102I 3052 GCTGAGC GC^T[ACGT]AGC
3343 GCTGAGC
BpuAI 793 GTCTTC GAAGAC(2/6)
889 GTCTTC
BsrFI 98 ACCGGC [GA]^CCGG[CT]
1481 ACCGGC
BstEII 2742 GGTGACC G^GT[ACGT]ACC
3637 GGTCACC
BstPI 2742 GGTGACC G^GT[ACGT]ACC
3637 GGTCACC
BstXI 1563 CCACAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3627 CCAAGATCCTGG
CelII 3052 GCTGAGC GC^T[ACGT]AGC
3343 GCTGAGC
Cfr10I 98 ACCGGC [GA]^CCGG[CT]
1481 ACCGGC
Eco47III 2052 AGCGCT AGC^GCT
2519 AGCGCT
EcoO65I 2742 GGTGACC G^GT[ACGT]ACC
3637 GGTCACC
PaeR7I 2672 CTCGAG C^TCGAG
2925 CTCGAG
SapI 2368 GAAGAGC GCTCTTC(1/4)
2426 GCTCTTC
SexAI 3356 ACCAGGT A^CC[AT]GGT
3434 ACCAGGT
StuI 43 AGGCCT AGG^CCT
858 AGGCCT
XcmI 593 CCACCTATACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2180 CCATCGTCCATGTGG
XhoI 2672 CTCGAG C^TCGAG
2925 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI Asp718I AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I
XhoII