(Circular) (Six-base) MAPSORT of: pFN21ASDA0482.seq Check: 2156 from: 1 to: 8599 pFN21ASDA0482 8599 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2533 2533 Size: 8599 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6409 278 Size: 53 83 186 936 4873 2468 Fragments arranged by size: 4873 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2718 5942 1937 Size: 781 3224 4594 Fragments arranged by size: 4594 3224 781 Acc65I G'GTAC_C Cuts at: 2774 2958 3930 5920 2774 Size: 184 972 1990 5453 Fragments arranged by size: 5453 1990 972 184 AclI AA'CG_TT Cuts at: 1563 6726 7099 1563 Size: 5163 373 3063 Fragments arranged by size: 5163 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2648 4177 4593 4872 5510 6662 8046 8314 Size: 1016 1529 416 279 638 1152 1384 268 Cuts at: 8314 1632 Size: 1917 Fragments arranged by size: 1917 1529 1384 1152 1016 638 416 279 268 AfeI AGC'GCT Cuts at: 4077 4544 4077 Size: 467 8132 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8402 AflIII A'CryG_T Cuts at: 1203 2610 2763 3429 7498 8475 1203 Size: 1407 153 666 4069 977 1327 Fragments arranged by size: 4069 1407 1327 977 666 153 AgeI A'CCGG_T Cuts at: 7430 7430 Size: 8599 AhdI GACnn_n'nnGTC Cuts at: 2377 7328 2377 Size: 4951 3648 AleI CACnn'nnGTG Cuts at: 1166 3025 4733 1166 Size: 1859 1708 5032 Fragments arranged by size: 5032 1859 1708 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3043 5899 1184 Size: 1859 2856 3884 Fragments arranged by size: 3884 2856 1859 AlwNI CAG_nnn'CTG Cuts at: 2391 2690 3487 4010 4043 6342 7914 2391 Size: 299 797 523 33 2299 1572 3076 Fragments arranged by size: 3076 2299 1572 797 523 299 33 ApaI G_GGCC'C Cuts at: 2112 3016 3196 3730 5052 2112 Size: 904 180 534 1322 5659 Fragments arranged by size: 5659 1322 904 534 180 ApaLI G'TGCA_C Cuts at: 3356 5573 6656 7812 3356 Size: 2217 1083 1156 4143 Fragments arranged by size: 4143 2217 1156 1083 ApoI r'AATT_y Cuts at: 1493 5908 6134 6286 8395 1493 Size: 4415 226 152 2109 1697 Fragments arranged by size: 4415 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7153 160 Size: 872 6121 1606 Fragments arranged by size: 6121 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8599 AvaI C'yCGr_G Cuts at: 1870 1943 2412 3723 4566 4695 4909 4948 5200 Size: 73 469 1311 843 129 214 39 252 Cuts at: 5200 5355 5604 5924 1870 Size: 155 249 320 4545 Fragments arranged by size: 4545 1311 843 469 320 252 249 214 155 129 73 39 BamHI G'GATC_C Cuts at: 2944 5404 5929 6296 2944 Size: 2460 525 367 5247 Fragments arranged by size: 5247 2460 525 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2774 2958 3560 3930 4124 Size: 325 217 627 987 184 602 370 194 Cuts at: 4124 5461 5920 7375 618 Size: 1337 459 1455 1842 Fragments arranged by size: 1842 1455 1337 987 627 602 459 370 325 217 194 184 BanII G_rGCy'C Cuts at: 1391 2112 2231 2315 2457 2793 3016 3196 3730 Size: 721 119 84 142 336 223 180 534 Cuts at: 3730 4136 4998 5052 5207 5216 5228 5611 5712 Size: 406 862 54 155 9 12 383 101 Cuts at: 5712 5852 5918 1391 Size: 140 66 4072 Fragments arranged by size: 4072 862 721 534 406 383 336 223 180 155 142 140 119 101 84 66 54 12 9 BbeI G_GCGC'C Cuts at: 3564 3564 Size: 8599 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2809 2905 928 Size: 971 910 96 6622 Fragments arranged by size: 6622 971 910 96 BbvCI CC'TCA_GC Cuts at: 2869 4003 4406 4465 5007 5266 5340 2869 Size: 1134 403 59 542 259 74 6128 Fragments arranged by size: 6128 1134 542 403 259 74 59 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4506 4540 5516 5550 6790 6824 1903 Size: 34 2569 34 976 34 1240 34 3678 Fragments arranged by size: 3678 2569 1240 976 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2668 3072 6491 7701 2668 Size: 404 3419 1210 3566 Fragments arranged by size: 3566 3419 1210 404 BclI T'GATC_A Cuts at: 1548 1548 Size: 8599 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4154 4494 5093 Size: 107 122 71 631 605 2482 340 599 Cuts at: 5093 7210 136 Size: 2117 1525 Fragments arranged by size: 2482 2117 1525 631 605 599 340 122 107 71 BglII A'GATC_T Cuts at: 3666 8594 3666 Size: 4928 3671 BlpI GC'TnA_GC Cuts at: 5076 5367 6016 5076 Size: 291 649 7659 Fragments arranged by size: 7659 649 291 Bme1580I G_kGCm'C Cuts at: 1790 2112 2861 3016 3196 3310 3360 3730 4740 Size: 322 749 155 180 114 50 370 1010 Cuts at: 4740 4837 5052 5466 5577 6660 7816 1790 Size: 97 215 414 111 1083 1156 2573 Fragments arranged by size: 2573 1156 1083 1010 749 414 370 322 215 180 155 114 111 97 50 BmgBI CAC'GTC Cuts at: 1318 2649 2764 3430 1318 Size: 1331 115 666 6487 Fragments arranged by size: 6487 1331 666 115 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3191 4676 5055 Size: 416 53 83 399 24 1774 1485 379 Cuts at: 5055 5573 5708 6330 7288 442 Size: 518 135 622 958 1753 Fragments arranged by size: 1774 1753 1485 958 622 518 416 399 379 135 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8599 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2617 4935 2617 Size: 2318 6281 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2294 2603 2648 2657 3385 3586 3833 3868 Size: 1055 309 45 9 728 201 247 35 Cuts at: 3868 3956 4823 4952 5009 5122 5384 5452 5726 Size: 88 867 129 57 113 262 68 274 Cuts at: 5726 7259 1239 Size: 1533 2579 Fragments arranged by size: 2579 1533 1055 867 728 309 274 262 247 201 129 113 88 68 57 45 35 9 Bpu10I CC'TnA_GC Cuts at: 2869 4003 4130 4406 4465 5007 5266 5340 2869 Size: 1134 127 276 59 542 259 74 6128 Fragments arranged by size: 6128 1134 542 276 259 127 74 59 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2441 6724 7589 7887 2441 Size: 4283 865 298 3153 Fragments arranged by size: 4283 3153 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2270 3007 4413 7262 882 Size: 617 771 737 1406 2849 2219 Fragments arranged by size: 2849 2219 1406 771 737 617 BsaAI yAC'GTr Cuts at: 493 1204 5395 5896 6390 493 Size: 711 4191 501 494 2702 Fragments arranged by size: 4191 2702 711 501 494 BsaBI GATnn'nnATC Cuts at: 3681 8593 3681 Size: 4912 3687 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2094 3561 4335 6406 Size: 53 83 186 936 561 1467 774 2071 Cuts at: 6406 6788 7423 275 Size: 382 635 1451 Fragments arranged by size: 2071 1467 1451 936 774 635 561 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5586 7032 7430 7704 7851 8262 5586 Size: 1446 398 274 147 411 5923 Fragments arranged by size: 5923 1446 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2225 2255 5162 5192 1178 Size: 30 165 30 822 30 2907 30 4585 Fragments arranged by size: 4585 2907 822 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2230 2233 2600 2645 2943 3875 3970 3973 Size: 1044 3 367 45 298 932 95 3 Cuts at: 3973 3976 3991 4683 4713 4908 5006 5792 5843 Size: 3 15 692 30 195 98 786 51 Cuts at: 5843 5846 1186 Size: 3 3939 Fragments arranged by size: 3939 1044 932 786 692 367 298 195 98 95 51 45 30 15 3 3 3 3 BseYI C'CCAG_C Cuts at: 2138 2324 3119 3163 3342 3449 3454 3533 3953 Size: 186 795 44 179 107 5 79 420 Cuts at: 3953 4282 4289 4294 4301 4324 4654 4707 4741 Size: 329 7 5 7 23 330 53 34 Cuts at: 4741 5345 7802 8298 2138 Size: 604 2457 496 2439 Fragments arranged by size: 2457 2439 795 604 496 420 330 329 186 179 107 79 53 44 34 23 7 7 5 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2567 3003 4361 5869 1163 Size: 573 831 436 1358 1508 3893 Fragments arranged by size: 3893 1508 1358 831 573 436 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6049 6810 7838 1540 Size: 275 186 4048 761 1028 2301 Fragments arranged by size: 4048 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2231 2300 2457 2643 2793 3204 3360 4791 4998 Size: 69 157 186 150 411 156 1431 207 Cuts at: 4998 5400 5577 5852 6660 6745 7816 2231 Size: 402 177 275 808 85 1071 3014 Fragments arranged by size: 3014 1431 1071 808 411 402 275 207 186 177 157 156 150 85 69 BsmI GAATG_Cn' Cuts at: 6110 6203 6110 Size: 93 8506 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2231 2300 2315 2457 2643 2793 Size: 399 322 119 69 15 142 186 150 Cuts at: 2793 2861 3016 3196 3204 3310 3360 3730 4136 Size: 68 155 180 8 106 50 370 406 Cuts at: 4136 4740 4791 4837 4998 5052 5207 5216 5228 Size: 604 51 46 161 54 155 9 12 Cuts at: 5228 5400 5466 5577 5611 5712 5852 5918 6660 Size: 172 66 111 34 101 140 66 742 Cuts at: 6660 6745 7816 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 604 406 399 370 322 186 180 172 161 155 155 150 142 140 119 111 106 101 85 69 68 66 66 54 51 50 46 34 15 12 9 8 BspEI T'CCGG_A Cuts at: 5586 8262 5586 Size: 2676 5923 BspHI T'CATG_A Cuts at: 2436 4248 5445 6488 8218 2436 Size: 1812 1197 1043 1730 2817 Fragments arranged by size: 2817 1812 1730 1197 1043 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2533 2864 3180 4269 5154 5954 844 Size: 1689 331 316 1089 885 800 3489 Fragments arranged by size: 3489 1689 1089 885 800 331 316 BsrBI CCG'CTC Cuts at: 1394 1823 2496 4138 5506 6486 1394 Size: 429 673 1642 1368 980 3507 Fragments arranged by size: 3507 1642 1368 980 673 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2176 2191 2334 7094 7268 65 Size: 1183 928 15 143 4760 174 1396 Fragments arranged by size: 4760 1396 1183 928 174 143 15 BsrFI r'CCGG_y Cuts at: 1700 1810 2121 3504 7243 7430 1700 Size: 110 311 1383 3739 187 2869 Fragments arranged by size: 3739 2869 1383 311 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8599 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8599 BssSI C'ACGA_G Cuts at: 6659 7671 6659 Size: 1012 7587 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8599 BstBI TT'CG_AA Cuts at: 2007 7403 2007 Size: 5396 3203 BstEII G'GTnAC_C Cuts at: 1184 4765 5660 1184 Size: 3581 895 4123 Fragments arranged by size: 4123 3581 895 BstXI CCAn_nnnn'nTGG Cuts at: 3593 5657 3593 Size: 2064 6535 BstYI r'GATC_y Cuts at: 1972 2944 2994 3498 3666 4060 5404 5653 5929 Size: 972 50 504 168 394 1344 249 276 Cuts at: 5929 6296 6681 6698 8150 8236 8248 8594 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1344 972 504 394 385 367 346 276 249 168 86 50 17 12 Bsu36I CC'TnA_GG Cuts at: 2245 2885 3219 3858 3926 2245 Size: 640 334 639 68 6918 Fragments arranged by size: 6918 640 639 334 68 BtgI C'CryG_G Cuts at: 513 1065 2604 3330 3540 4100 4492 5712 513 Size: 552 1539 726 210 560 392 1220 3400 Fragments arranged by size: 3400 1539 1220 726 560 552 392 210 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7860 Fragments arranged by size: 7860 683 56 BtsI GCAGTG_nn' Cuts at: 2404 2579 2829 4522 5047 5761 6115 6909 6929 Size: 175 250 1693 525 714 354 794 20 Cuts at: 6929 2404 Size: 4074 Fragments arranged by size: 4074 1693 794 714 525 354 250 175 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8599 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3831 3866 529 Size: 35 3267 35 5262 Fragments arranged by size: 5262 3267 35 35 DraI TTT'AAA Cuts at: 5903 5903 Size: 8599 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5441 7606 809 Size: 507 4125 2165 1802 Fragments arranged by size: 4125 2165 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2508 4146 Size: 54 1425 50 275 18 315 363 1638 Cuts at: 4146 5424 6046 6935 8564 8 Size: 1278 622 889 1629 43 Fragments arranged by size: 1638 1629 1425 1278 889 622 363 315 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6046 1537 Size: 275 4234 4090 Fragments arranged by size: 4234 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3134 4386 4456 4719 5335 5684 5712 6529 Size: 1781 1252 70 263 616 349 28 817 Cuts at: 6529 1353 Size: 3423 Fragments arranged by size: 3423 1781 1252 817 616 349 263 70 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2960 4053 4172 5111 7186 7560 Size: 1064 138 1371 1093 119 939 2075 374 Cuts at: 7560 7706 387 Size: 146 1280 Fragments arranged by size: 2075 1371 1280 1093 1064 939 374 146 138 119 EcoICRI GAG'CTC Cuts at: 2229 2455 2791 4996 5850 2229 Size: 226 336 2205 854 4978 Fragments arranged by size: 4978 2205 854 336 226 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2294 2603 2648 2657 3385 3586 3833 Size: 393 662 309 45 9 728 201 247 Cuts at: 3833 3868 3956 4177 4593 4823 4872 4952 5009 Size: 35 88 221 416 230 49 80 57 Cuts at: 5009 5122 5384 5452 5510 5726 6662 7259 8046 Size: 113 262 68 58 216 936 597 787 Cuts at: 8046 8314 1239 Size: 268 1524 Fragments arranged by size: 1524 936 787 728 662 597 416 393 309 268 262 247 230 221 216 201 113 88 80 68 58 57 49 45 35 9 EcoNI CCTnn'n_nnAGG Cuts at: 2130 2585 3131 3176 3422 3759 4698 4851 5874 Size: 455 546 45 246 337 939 153 1023 Cuts at: 5874 2130 Size: 4855 Fragments arranged by size: 4855 1023 939 546 455 337 246 153 45 EcoO109I rG'GnC_Cy Cuts at: 2059 2109 2135 2502 2865 2933 2951 3012 3013 Size: 50 26 367 363 68 18 61 1 Cuts at: 3013 3181 3192 3193 3578 3597 3632 3781 3838 Size: 168 11 1 385 19 35 149 57 Cuts at: 3838 4258 4703 4856 5030 5049 2059 Size: 420 445 153 174 19 5609 Fragments arranged by size: 5609 445 420 385 367 363 174 168 153 149 68 61 57 50 35 26 19 19 18 11 1 1 EcoRI G'AATT_C Cuts at: 5908 6286 5908 Size: 378 8221 EcoRV GAT'ATC Cuts at: 3478 3478 Size: 8599 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2929 3397 2929 Size: 468 8131 FspI TGC'GCA Cuts at: 7105 7105 Size: 8599 HaeII r_GCGC'y Cuts at: 1678 2482 3564 4079 4546 4580 7746 1678 Size: 804 1082 515 467 34 3166 2531 Fragments arranged by size: 3166 2531 1082 804 515 467 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2363 2395 2666 2698 4018 Size: 32 135 32 791 32 271 32 1320 Cuts at: 4018 4050 4052 4084 5162 5194 5465 5497 7240 Size: 32 2 32 1078 32 271 32 1743 Cuts at: 7240 7272 7314 7346 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1743 1320 1078 791 271 271 135 42 32 32 32 32 32 32 32 32 32 32 2 HincII GTy'rAC Cuts at: 669 1657 1938 5943 6189 669 Size: 988 281 4005 246 3079 Fragments arranged by size: 4005 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2459 748 Size: 1711 6888 HpaI GTT'AAC Cuts at: 6189 6189 Size: 8599 KasI G'GCGC_C Cuts at: 3560 3560 Size: 8599 KpnI G_GTAC'C Cuts at: 2778 2962 3934 5924 2778 Size: 184 972 1990 5453 Fragments arranged by size: 5453 1990 972 184 MfeI C'AATT_G Cuts at: 6198 6198 Size: 8599 MluI A'CGCG_T Cuts at: 8475 8475 Size: 8599 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2217 4218 7713 7897 1305 Size: 912 2001 3495 184 2007 Fragments arranged by size: 3495 2007 2001 912 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2510 4148 5426 10 Size: 54 1425 343 678 1638 1278 3183 Fragments arranged by size: 3183 1638 1425 1278 678 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2609 3025 4247 4250 4733 5667 6557 Size: 648 1443 416 1222 3 483 934 890 Cuts at: 6557 6916 7075 518 Size: 359 159 2042 Fragments arranged by size: 2042 1443 1222 934 890 648 483 416 359 159 3 MspA1I CmG'CkG Cuts at: 1606 2224 3123 3342 3888 4658 4745 4987 5554 Size: 618 899 219 546 770 87 242 567 Cuts at: 5554 5745 6015 6692 7840 8085 1606 Size: 191 270 677 1148 245 2120 Fragments arranged by size: 2120 1148 899 770 677 618 567 546 270 245 242 219 191 87 NarI GG'CG_CC Cuts at: 3561 3561 Size: 8599 NcoI C'CATG_G Cuts at: 513 1065 2604 3330 5712 513 Size: 552 1539 726 2382 3400 Fragments arranged by size: 3400 2382 1539 726 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8599 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8599 NotI GC'GGCC_GC Cuts at: 6046 6046 Size: 8599 NspI r_CATG'y Cuts at: 1132 1229 2614 5957 7502 1132 Size: 97 1385 3343 1545 2229 Fragments arranged by size: 3343 2229 1545 1385 97 PasI CC'CwG_GG Cuts at: 1348 2105 3593 5033 1348 Size: 757 1488 1440 4914 Fragments arranged by size: 4914 1488 1440 757 PciI A'CATG_T Cuts at: 2610 7498 2610 Size: 4888 3711 PflMI CCAn_nnn'nTGG Cuts at: 1108 2105 2809 3139 4623 1108 Size: 997 704 330 1484 5084 Fragments arranged by size: 5084 1484 997 704 330 PfoI T'CCnGG_A Cuts at: 3132 3480 5400 3132 Size: 348 1920 6331 Fragments arranged by size: 6331 1920 348 PmeI GTTT'AAAC Cuts at: 5903 5903 Size: 8599 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3416 3448 6668 6700 8181 8213 3416 Size: 32 3220 32 1481 32 3802 Fragments arranged by size: 3802 3220 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2059 2502 2933 3181 3597 4258 2059 Size: 443 431 248 416 661 6400 Fragments arranged by size: 6400 661 443 431 416 248 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8599 PsiI TTA'TAA Cuts at: 6169 6169 Size: 8599 PspOMI G'GGCC_C Cuts at: 2108 3012 3192 3726 5048 2108 Size: 904 180 534 1322 5659 Fragments arranged by size: 5659 1322 904 534 180 PspXI vC'TCGA_Gb Cuts at: 4695 4695 Size: 8599 PstI C_TGCA'G Cuts at: 830 2396 2520 3264 3519 4986 5951 830 Size: 1566 124 744 255 1467 965 3478 Fragments arranged by size: 3478 1566 1467 965 744 255 124 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8599 PvuII CAG'CTG Cuts at: 3123 3342 3888 4745 4987 3123 Size: 219 546 857 242 6735 Fragments arranged by size: 6735 857 546 242 219 SacI G_AGCT'C Cuts at: 2231 2457 2793 4998 5852 2231 Size: 226 336 2205 854 4978 Fragments arranged by size: 4978 2205 854 336 226 SalI G'TCGA_C Cuts at: 1936 5941 1936 Size: 4005 4594 SanDI GG'GwC_CC Cuts at: 2059 2502 2059 Size: 443 8156 SapI GCTCTTCn'nnn_ Cuts at: 4386 4456 4386 Size: 70 8529 SbfI CC_TGCA'GG Cuts at: 5951 5951 Size: 8599 ScaI AGT'ACT Cuts at: 6847 8559 6847 Size: 1712 6887 SexAI A'CCwGG_T Cuts at: 5379 5457 5379 Size: 78 8521 SfcI C'TryA_G Cuts at: 826 1045 2341 2392 2516 3260 3515 3759 4982 Size: 219 1296 51 124 744 255 244 1223 Cuts at: 4982 5871 5947 7082 7763 7954 826 Size: 889 76 1135 681 191 1471 Fragments arranged by size: 1471 1296 1223 1135 889 744 681 255 244 219 191 124 76 51 SfoI GGC'GCC Cuts at: 3562 3562 Size: 8599 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8599 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8599 SmaI CCC'GGG Cuts at: 1872 2414 5926 1872 Size: 542 3512 4545 Fragments arranged by size: 4545 3512 542 SmlI C'TyrA_G Cuts at: 820 1017 1943 2456 4695 4948 6703 7604 7866 Size: 197 926 513 2239 253 1755 901 262 Cuts at: 7866 820 Size: 1553 Fragments arranged by size: 2239 1755 1553 926 901 513 262 253 197 SnaBI TAC'GTA Cuts at: 493 5896 6390 493 Size: 5403 494 2702 Fragments arranged by size: 5403 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8599 SphI G_CATG'C Cuts at: 1132 5957 1132 Size: 4825 3774 SrfI GCCC'GGGC Cuts at: 2414 2414 Size: 8599 SspI AAT'ATT Cuts at: 5 52 6523 5 Size: 47 6471 2081 Fragments arranged by size: 6471 2081 47 StuI AGG'CCT Cuts at: 1517 2068 2883 1517 Size: 551 815 7233 Fragments arranged by size: 7233 815 551 StyI C'CwwG_G Cuts at: 513 1065 2604 3330 3777 3849 4359 4471 4782 Size: 552 1539 726 447 72 510 112 311 Cuts at: 4782 5208 5712 5785 5796 6038 513 Size: 426 504 73 11 242 3074 Fragments arranged by size: 3074 1539 726 552 510 504 447 426 311 242 112 73 72 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2338 3133 3240 5555 5663 6626 6643 Size: 1255 758 795 107 2315 108 963 17 Cuts at: 6643 6796 6981 7426 325 Size: 153 185 445 1498 Fragments arranged by size: 2315 1498 1255 963 795 758 445 185 153 108 107 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2655 6845 8557 Size: 274 80 33 51 1443 678 4190 1712 Cuts at: 8557 96 Size: 138 Fragments arranged by size: 4190 1712 1443 678 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2225 2257 4514 4546 5638 5670 8559 8591 2225 Size: 32 2257 32 1092 32 2889 32 2233 Fragments arranged by size: 2889 2257 2233 1092 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8599 XbaI T'CTAG_A Cuts at: 2207 5935 2207 Size: 3728 4871 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2623 4210 2623 Size: 1587 7012 XhoI C'TCGA_G Cuts at: 1943 4695 4948 1943 Size: 2752 253 5594 Fragments arranged by size: 5594 2752 253 XmaI C'CCGG_G Cuts at: 1870 2412 5924 1870 Size: 542 3512 4545 Fragments arranged by size: 4545 3512 542 XmnI GAAnn'nnTTC Cuts at: 6728 6728 Size: 8599 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6407 276 Size: 53 83 186 936 4873 2468 Fragments arranged by size: 4873 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacII SfiI SwaI