Restriction map for
pFN21ASDA0482
(Linear) (Six-base) MAPSORT of: KIAA0482.seq Check: 8317 from: 1 to: 3870
KIAA0482.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 510 3870
Size: 510 3360
AccI GT'mk_AC
Cuts at: 0 695 3870
Size: 695 3175
Acc65I G'GTAC_C
Cuts at: 0 751 935 1907 3870
Size: 751 184 972 1963
Fragments arranged by size:
1963 972 751 184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 2154 2570 2849 3487 3870
Size: 625 1529 416 279 638 383
Fragments arranged by size:
1529 638 625 416 383 279
AfeI AGC'GCT
Cuts at: 0 2054 2521 3870
Size: 2054 467 1349
Fragments arranged by size:
2054 1349 467
AflIII A'CryG_T
Cuts at: 0 587 740 1406 3870
Size: 587 153 666 2464
Fragments arranged by size:
2464 666 587 153
AhdI GACnn_n'nnGTC
Cuts at: 0 354 3870
Size: 354 3516
AleI CACnn'nnGTG
Cuts at: 0 1002 2710 3870
Size: 1002 1708 1160
Fragments arranged by size:
1708 1160 1002
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1020 3870
Size: 1020 2850
AlwNI CAG_nnn'CTG
Cuts at: 0 368 667 1464 1987 2020 3870
Size: 368 299 797 523 33 1850
Fragments arranged by size:
1850 797 523 368 299 33
ApaI G_GGCC'C
Cuts at: 0 89 993 1173 1707 3029 3870
Size: 89 904 180 534 1322 841
Fragments arranged by size:
1322 904 841 534 180 89
ApaLI G'TGCA_C
Cuts at: 0 1333 3550 3870
Size: 1333 2217 320
Fragments arranged by size:
2217 1333 320
AvaI C'yCGr_G
Cuts at: 0 389 1700 2543 2672 2886 2925 3177 3332
Size: 389 1311 843 129 214 39 252 155
Cuts at: 3332 3581 3870
Size: 249 289
Fragments arranged by size:
1311 843 389 289 252 249 214 155
129 39
BamHI G'GATC_C
Cuts at: 0 921 3381 3870
Size: 921 2460 489
Fragments arranged by size:
2460 921 489
BanI G'GyrC_C
Cuts at: 0 751 935 1537 1907 2101 3438 3870
Size: 751 184 602 370 194 1337 432
Fragments arranged by size:
1337 751 602 432 370 194 184
BanII G_rGCy'C
Cuts at: 0 89 208 292 434 770 993 1173 1707
Size: 89 119 84 142 336 223 180 534
Cuts at: 1707 2113 2975 3029 3184 3193 3205 3588 3689
Size: 406 862 54 155 9 12 383 101
Cuts at: 3689 3829 3870
Size: 140 41
Fragments arranged by size:
862 534 406 383 336 223 180 155
142 140 119 101 89 84 54 41
12 9
BbeI G_GCGC'C
Cuts at: 0 1541 3870
Size: 1541 2329
BbsI GAAGACnn'nnnn_
Cuts at: 0 786 882 3870
Size: 786 96 2988
Fragments arranged by size:
2988 786 96
BbvCI CC'TCA_GC
Cuts at: 0 846 1980 2383 2442 2984 3243 3317 3870
Size: 846 1134 403 59 542 259 74 553
Fragments arranged by size:
1134 846 553 542 403 259 74 59
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2483 2517 3493 3527 3870
Size: 2483 34 976 34 343
Fragments arranged by size:
2483 976 343 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 645 1049 3870
Size: 645 404 2821
Fragments arranged by size:
2821 645 404
BglI GCCn_nnn'nGGC
Cuts at: 0 2131 2471 3070 3870
Size: 2131 340 599 800
Fragments arranged by size:
2131 800 599 340
BglII A'GATC_T
Cuts at: 0 1643 3870
Size: 1643 2227
BlpI GC'TnA_GC
Cuts at: 0 3053 3344 3870
Size: 3053 291 526
Fragments arranged by size:
3053 526 291
Bme1580I G_kGCm'C
Cuts at: 0 89 838 993 1173 1287 1337 1707 2717
Size: 89 749 155 180 114 50 370 1010
Cuts at: 2717 2814 3029 3443 3554 3870
Size: 97 215 414 111 316
Fragments arranged by size:
1010 749 414 370 316 215 180 155
114 111 97 89 50
BmgBI CAC'GTC
Cuts at: 0 626 741 1407 3870
Size: 626 115 666 2463
Fragments arranged by size:
2463 666 626 115
BmrI ACTGGGnnnn_n'
Cuts at: 0 1168 2653 3032 3550 3685 3870
Size: 1168 1485 379 518 135 185
Fragments arranged by size:
1485 1168 518 379 185 135
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 594 2912 3870
Size: 594 2318 958
Fragments arranged by size:
2318 958 594
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 580 625 634 1362 1563 1810 1845
Size: 271 309 45 9 728 201 247 35
Cuts at: 1845 1933 2800 2929 2986 3099 3361 3429 3703
Size: 88 867 129 57 113 262 68 274
Cuts at: 3703 3870
Size: 167
Fragments arranged by size:
867 728 309 274 271 262 247 201
167 129 113 88 68 57 45 35
9
Bpu10I CC'TnA_GC
Cuts at: 0 846 1980 2107 2383 2442 2984 3243 3317
Size: 846 1134 127 276 59 542 259 74
Cuts at: 3317 3870
Size: 553
Fragments arranged by size:
1134 846 553 542 276 259 127 74
59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 3870
Size: 418 3452
BsaI GGTCTCn'nnnn_
Cuts at: 0 247 984 2390 3870
Size: 247 737 1406 1480
Fragments arranged by size:
1480 1406 737 247
BsaAI yAC'GTr
Cuts at: 0 3372 3870
Size: 3372 498
BsaBI GATnn'nnATC
Cuts at: 0 1658 3870
Size: 1658 2212
BsaHI Gr'CG_yC
Cuts at: 0 71 1538 2312 3870
Size: 71 1467 774 1558
Fragments arranged by size:
1558 1467 774 71
BsaWI w'CCGG_w
Cuts at: 0 3563 3870
Size: 3563 307
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 202 232 3139 3169 3870
Size: 202 30 2907 30 701
Fragments arranged by size:
2907 701 202 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 207 210 577 622 920 1852 1947 1950
Size: 207 3 367 45 298 932 95 3
Cuts at: 1950 1953 1968 2660 2690 2885 2983 3769 3820
Size: 3 15 692 30 195 98 786 51
Cuts at: 3820 3823 3870
Size: 3 47
Fragments arranged by size:
932 786 692 367 298 207 195 98
95 51 47 45 30 15 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 115 301 1096 1140 1319 1426 1431 1510
Size: 115 186 795 44 179 107 5 79
Cuts at: 1510 1930 2259 2266 2271 2278 2301 2631 2684
Size: 420 329 7 5 7 23 330 53
Cuts at: 2684 2718 3322 3870
Size: 34 604 548
Fragments arranged by size:
795 604 548 420 330 329 186 179
115 107 79 53 44 34 23 7
7 5 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 980 2338 3846 3870
Size: 544 436 1358 1508 24
Fragments arranged by size:
1508 1358 544 436 24
BsiHKAI G_wGCw'C
Cuts at: 0 208 277 434 620 770 1181 1337 2768
Size: 208 69 157 186 150 411 156 1431
Cuts at: 2768 2975 3377 3554 3829 3870
Size: 207 402 177 275 41
Fragments arranged by size:
1431 411 402 275 208 207 186 177
157 156 150 69 41
Bsp1286I G_dGCh'C
Cuts at: 0 89 208 277 292 434 620 770 838
Size: 89 119 69 15 142 186 150 68
Cuts at: 838 993 1173 1181 1287 1337 1707 2113 2717
Size: 155 180 8 106 50 370 406 604
Cuts at: 2717 2768 2814 2975 3029 3184 3193 3205 3377
Size: 51 46 161 54 155 9 12 172
Cuts at: 3377 3443 3554 3588 3689 3829 3870
Size: 66 111 34 101 140 41
Fragments arranged by size:
604 406 370 186 180 172 161 155
155 150 142 140 119 111 106 101
89 69 68 66 54 51 50 46
41 34 15 12 9 8
BspEI T'CCGG_A
Cuts at: 0 3563 3870
Size: 3563 307
BspHI T'CATG_A
Cuts at: 0 413 2225 3422 3870
Size: 413 1812 1197 448
Fragments arranged by size:
1812 1197 448 413
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 510 841 1157 2246 3131 3870
Size: 510 331 316 1089 885 739
Fragments arranged by size:
1089 885 739 510 331 316
BsrBI CCG'CTC
Cuts at: 0 473 2115 3483 3870
Size: 473 1642 1368 387
Fragments arranged by size:
1642 1368 473 387
BsrDI GCAATG_nn'
Cuts at: 0 153 168 311 3870
Size: 153 15 143 3559
Fragments arranged by size:
3559 153 143 15
BsrFI r'CCGG_y
Cuts at: 0 98 1481 3870
Size: 98 1383 2389
Fragments arranged by size:
2389 1383 98
BstEII G'GTnAC_C
Cuts at: 0 2742 3637 3870
Size: 2742 895 233
Fragments arranged by size:
2742 895 233
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1570 3634 3870
Size: 1570 2064 236
Fragments arranged by size:
2064 1570 236
BstYI r'GATC_y
Cuts at: 0 921 971 1475 1643 2037 3381 3630 3870
Size: 921 50 504 168 394 1344 249 240
Fragments arranged by size:
1344 921 504 394 249 240 168 50
Bsu36I CC'TnA_GG
Cuts at: 0 222 862 1196 1835 1903 3870
Size: 222 640 334 639 68 1967
Fragments arranged by size:
1967 640 639 334 222 68
BtgI C'CryG_G
Cuts at: 0 581 1307 1517 2077 2469 3689 3870
Size: 581 726 210 560 392 1220 181
Fragments arranged by size:
1220 726 581 560 392 210 181
BtsI GCAGTG_nn'
Cuts at: 0 381 556 806 2499 3024 3738 3870
Size: 381 175 250 1693 525 714 132
Fragments arranged by size:
1693 714 525 381 250 175 132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1808 1843 3870
Size: 1808 35 2027
Fragments arranged by size:
2027 1808 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 3418 3870
Size: 3418 452
EaeI y'GGCC_r
Cuts at: 0 122 485 2123 3401 3870
Size: 122 363 1638 1278 469
Fragments arranged by size:
1638 1278 469 363 122
EarI CTCTTCn'nnn_
Cuts at: 0 1111 2363 2433 2696 3312 3661 3689 3870
Size: 1111 1252 70 263 616 349 28 181
Fragments arranged by size:
1252 1111 616 349 263 181 70 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 937 2030 2149 3088 3870
Size: 937 1093 119 939 782
Fragments arranged by size:
1093 939 937 782 119
EcoICRI GAG'CTC
Cuts at: 0 206 432 768 2973 3827 3870
Size: 206 226 336 2205 854 43
Fragments arranged by size:
2205 854 336 226 206 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 580 625 634 1362 1563 1810 1845
Size: 271 309 45 9 728 201 247 35
Cuts at: 1845 1933 2154 2570 2800 2849 2929 2986 3099
Size: 88 221 416 230 49 80 57 113
Cuts at: 3099 3361 3429 3487 3703 3870
Size: 262 68 58 216 167
Fragments arranged by size:
728 416 309 271 262 247 230 221
216 201 167 113 88 80 68 58
57 49 45 35 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 107 562 1108 1153 1399 1736 2675 2828
Size: 107 455 546 45 246 337 939 153
Cuts at: 2828 3851 3870
Size: 1023 19
Fragments arranged by size:
1023 939 546 455 337 246 153 107
45 19
EcoO109I rG'GnC_Cy
Cuts at: 0 36 86 112 479 842 910 928 989
Size: 36 50 26 367 363 68 18 61
Cuts at: 989 990 1158 1169 1170 1555 1574 1609 1758
Size: 1 168 11 1 385 19 35 149
Cuts at: 1758 1815 2235 2680 2833 3007 3026 3870
Size: 57 420 445 153 174 19 844
Fragments arranged by size:
844 445 420 385 367 363 174 168
153 149 68 61 57 50 36 35
26 19 19 18 11 1 1
EcoRV GAT'ATC
Cuts at: 0 1455 3870
Size: 1455 2415
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 906 1374 3870
Size: 906 468 2496
Fragments arranged by size:
2496 906 468
HaeII r_GCGC'y
Cuts at: 0 459 1541 2056 2523 2557 3870
Size: 459 1082 515 467 34 1313
Fragments arranged by size:
1313 1082 515 467 459 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 643 675 1995 2027 2029 2061
Size: 340 32 271 32 1320 32 2 32
Cuts at: 2061 3139 3171 3442 3474 3870
Size: 1078 32 271 32 396
Fragments arranged by size:
1320 1078 396 340 271 271 32 32
32 32 32 32 2
HindIII A'AGCT_T
Cuts at: 0 436 3870
Size: 436 3434
KasI G'GCGC_C
Cuts at: 0 1537 3870
Size: 1537 2333
KpnI G_GTAC'C
Cuts at: 0 755 939 1911 3870
Size: 755 184 972 1959
Fragments arranged by size:
1959 972 755 184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 2195 3870
Size: 194 2001 1675
Fragments arranged by size:
2001 1675 194
MscI TGG'CCA
Cuts at: 0 487 2125 3403 3870
Size: 487 1638 1278 467
Fragments arranged by size:
1638 1278 487 467
MslI CAynn'nnrTG
Cuts at: 0 586 1002 2224 2227 2710 3644 3870
Size: 586 416 1222 3 483 934 226
Fragments arranged by size:
1222 934 586 483 416 226 3
MspA1I CmG'CkG
Cuts at: 0 201 1100 1319 1865 2635 2722 2964 3531
Size: 201 899 219 546 770 87 242 567
Cuts at: 3531 3722 3870
Size: 191 148
Fragments arranged by size:
899 770 567 546 242 219 201 191
148 87
NarI GG'CG_CC
Cuts at: 0 1538 3870
Size: 1538 2332
NcoI C'CATG_G
Cuts at: 0 581 1307 3689 3870
Size: 581 726 2382 181
Fragments arranged by size:
2382 726 581 181
NspI r_CATG'y
Cuts at: 0 591 3870
Size: 591 3279
PasI CC'CwG_GG
Cuts at: 0 82 1570 3010 3870
Size: 82 1488 1440 860
Fragments arranged by size:
1488 1440 860 82
PciI A'CATG_T
Cuts at: 0 587 3870
Size: 587 3283
PflMI CCAn_nnn'nTGG
Cuts at: 0 82 786 1116 2600 3870
Size: 82 704 330 1484 1270
Fragments arranged by size:
1484 1270 704 330 82
PfoI T'CCnGG_A
Cuts at: 0 1109 1457 3377 3870
Size: 1109 348 1920 493
Fragments arranged by size:
1920 1109 493 348
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1393 1425 3870
Size: 1393 32 2445
Fragments arranged by size:
2445 1393 32
PpuMI rG'GwC_Cy
Cuts at: 0 36 479 910 1158 1574 2235 3870
Size: 36 443 431 248 416 661 1635
Fragments arranged by size:
1635 661 443 431 416 248 36
PspOMI G'GGCC_C
Cuts at: 0 85 989 1169 1703 3025 3870
Size: 85 904 180 534 1322 845
Fragments arranged by size:
1322 904 845 534 180 85
PspXI vC'TCGA_Gb
Cuts at: 0 2672 3870
Size: 2672 1198
PstI C_TGCA'G
Cuts at: 0 373 497 1241 1496 2963 3870
Size: 373 124 744 255 1467 907
Fragments arranged by size:
1467 907 744 373 255 124
PvuII CAG'CTG
Cuts at: 0 1100 1319 1865 2722 2964 3870
Size: 1100 219 546 857 242 906
Fragments arranged by size:
1100 906 857 546 242 219
SacI G_AGCT'C
Cuts at: 0 208 434 770 2975 3829 3870
Size: 208 226 336 2205 854 41
Fragments arranged by size:
2205 854 336 226 208 41
SanDI GG'GwC_CC
Cuts at: 0 36 479 3870
Size: 36 443 3391
Fragments arranged by size:
3391 443 36
SapI GCTCTTCn'nnn_
Cuts at: 0 2363 2433 3870
Size: 2363 70 1437
Fragments arranged by size:
2363 1437 70
SexAI A'CCwGG_T
Cuts at: 0 3356 3434 3870
Size: 3356 78 436
Fragments arranged by size:
3356 436 78
SfcI C'TryA_G
Cuts at: 0 318 369 493 1237 1492 1736 2959 3848
Size: 318 51 124 744 255 244 1223 889
Cuts at: 3848 3870
Size: 22
Fragments arranged by size:
1223 889 744 318 255 244 124 51
22
SfoI GGC'GCC
Cuts at: 0 1539 3870
Size: 1539 2331
SmaI CCC'GGG
Cuts at: 0 391 3870
Size: 391 3479
SmlI C'TyrA_G
Cuts at: 0 433 2672 2925 3870
Size: 433 2239 253 945
Fragments arranged by size:
2239 945 433 253
SrfI GCCC'GGGC
Cuts at: 0 391 3870
Size: 391 3479
StuI AGG'CCT
Cuts at: 0 45 860 3870
Size: 45 815 3010
Fragments arranged by size:
3010 815 45
StyI C'CwwG_G
Cuts at: 0 581 1307 1754 1826 2336 2448 2759 3185
Size: 581 726 447 72 510 112 311 426
Cuts at: 3185 3689 3762 3773 3870
Size: 504 73 11 97
Fragments arranged by size:
726 581 510 504 447 426 311 112
97 73 72 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 315 1110 1217 3532 3640 3870
Size: 315 795 107 2315 108 230
Fragments arranged by size:
2315 795 315 230 108 107
TatI w'GTAC_w
Cuts at: 0 632 3870
Size: 632 3238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 202 234 2491 2523 3615 3647 3870
Size: 202 32 2257 32 1092 32 223
Fragments arranged by size:
2257 1092 223 202 32 32 32
XbaI T'CTAG_A
Cuts at: 0 184 3870
Size: 184 3686
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 600 2187 3870
Size: 600 1587 1683
Fragments arranged by size:
1683 1587 600
XhoI C'TCGA_G
Cuts at: 0 2672 2925 3870
Size: 2672 253 945
Fragments arranged by size:
2672 945 253
XmaI C'CCGG_G
Cuts at: 0 389 3870
Size: 389 3481
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FalI HaeII Hin4I HindIII KasI KpnI MmeI MscI
MslI MspA1I NarI NcoI NspI PasI PciI PflMI
PfoI PpiI PpuMI PspOMI PspXI PstI PvuII SacI
SanDI SapI SexAI SfcI SfoI SmaI SmlI SrfI
StuI StyI TaqII TatI TstI XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AclI AflII AgeI AloI ApoI AscI AseI
AsiSI AvrII BaeI BclI BfrBI BmtI BsiEI BsiWI
BsmI BsmBI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
BtgZI ClaI DraI DraIII EagI EcoRI FseI FspI
FspAI HincII HpaI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PmeI PmlI PshAI
PsiI PsrI PvuI RsrII SacII SalI SbfI ScaI
SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI
Tth111I XmnI ZraI