(Circular) (Six-base) MAPSORT of: pFN21ASDA0479.seq Check: 8886 from: 1 to: 5650 pFN21ASDA0479 5650 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3460 278 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2993 1937 Size: 1056 4594 Acc65I G'GTAC_C Cuts at: 2971 2971 Size: 5650 AclI AA'CG_TT Cuts at: 1563 3777 4150 1563 Size: 2214 373 3063 Fragments arranged by size: 3063 2214 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3713 5097 5365 1632 Size: 2081 1384 268 1917 Fragments arranged by size: 2081 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5453 AflIII A'CryG_T Cuts at: 1203 4549 5526 1203 Size: 3346 977 1327 Fragments arranged by size: 3346 1327 977 AgeI A'CCGG_T Cuts at: 4481 4481 Size: 5650 AhdI GACnn_n'nnGTC Cuts at: 4379 4379 Size: 5650 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5650 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2594 1184 Size: 1410 4240 AlwNI CAG_nnn'CTG Cuts at: 3393 4965 3393 Size: 1572 4078 ApaLI G'TGCA_C Cuts at: 3707 4863 3707 Size: 1156 4494 ApoI r'AATT_y Cuts at: 1493 2248 2959 3185 3337 5446 1493 Size: 755 711 226 152 2109 1697 Fragments arranged by size: 2109 1697 755 711 226 152 AseI AT'TA_AT Cuts at: 160 1032 4204 160 Size: 872 3172 1606 Fragments arranged by size: 3172 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 AvaI C'yCGr_G Cuts at: 1870 1943 2715 2975 1870 Size: 73 772 260 4545 Fragments arranged by size: 4545 772 260 73 BamHI G'GATC_C Cuts at: 2604 2980 3347 2604 Size: 376 367 4907 Fragments arranged by size: 4907 376 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2214 2710 2971 4426 618 Size: 325 217 627 427 496 261 1455 1842 Fragments arranged by size: 1842 1455 627 496 427 325 261 217 BanII G_rGCy'C Cuts at: 1391 2658 2969 1391 Size: 1267 311 4072 Fragments arranged by size: 4072 1267 311 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4679 BbvCI CC'TCA_GC Cuts at: 2501 2501 Size: 5650 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3841 3875 1903 Size: 34 1904 34 3678 Fragments arranged by size: 3678 1904 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3542 4752 3542 Size: 1210 4440 BclI T'GATC_A Cuts at: 1548 1548 Size: 5650 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4261 136 Size: 107 122 71 631 605 2589 1525 Fragments arranged by size: 2589 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2475 5645 2475 Size: 3170 2480 BlpI GC'TnA_GC Cuts at: 3067 3067 Size: 5650 Bme1580I G_kGCm'C Cuts at: 1790 2094 2573 2715 3711 4867 1790 Size: 304 479 142 996 1156 2573 Fragments arranged by size: 2573 1156 996 479 304 142 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5650 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3381 4339 442 Size: 416 53 83 399 24 1964 958 1753 Fragments arranged by size: 1964 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5650 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2516 2516 Size: 5650 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2654 4310 1239 Size: 1415 1656 2579 Fragments arranged by size: 2579 1656 1415 Bpu10I CC'TnA_GC Cuts at: 2501 2501 Size: 5650 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3775 4640 4938 3775 Size: 865 298 4487 Fragments arranged by size: 4487 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2024 2411 4313 882 Size: 617 525 387 1902 2219 Fragments arranged by size: 2219 1902 617 525 387 BsaAI yAC'GTr Cuts at: 493 1204 2947 3441 493 Size: 711 1743 494 2702 Fragments arranged by size: 2702 1743 711 494 BsaBI GATnn'nnATC Cuts at: 5644 5644 Size: 5650 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3457 3839 4474 275 Size: 53 83 186 936 1924 382 635 1451 Fragments arranged by size: 1924 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4083 4481 4755 4902 5313 4083 Size: 398 274 147 411 4420 Fragments arranged by size: 4420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5425 Fragments arranged by size: 5425 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2743 1186 Size: 1557 4093 BseYI C'CCAG_C Cuts at: 2424 2889 4853 5349 2424 Size: 465 1964 496 2725 Fragments arranged by size: 2725 1964 496 465 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2111 1163 Size: 573 375 4702 Fragments arranged by size: 4702 573 375 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3100 3861 4889 1540 Size: 275 186 1099 761 1028 2301 Fragments arranged by size: 2301 1099 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3711 3796 4867 3711 Size: 85 1071 4494 Fragments arranged by size: 4494 1071 85 BsmI GAATG_Cn' Cuts at: 2563 3161 3254 2563 Size: 598 93 4959 Fragments arranged by size: 4959 598 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2225 2225 Size: 5650 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2573 2658 2715 2969 3711 3796 Size: 399 304 479 85 57 254 742 85 Cuts at: 3796 4867 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 479 399 304 254 85 85 57 BspEI T'CCGG_A Cuts at: 5313 5313 Size: 5650 BspHI T'CATG_A Cuts at: 2337 2742 3539 5269 2337 Size: 405 797 1730 2718 Fragments arranged by size: 2718 1730 797 405 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2315 2637 3005 844 Size: 1471 322 368 3489 Fragments arranged by size: 3489 1471 368 322 BsrBI CCG'CTC Cuts at: 1394 1823 3537 1394 Size: 429 1714 3507 Fragments arranged by size: 3507 1714 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4145 4319 65 Size: 1183 2897 174 1396 Fragments arranged by size: 2897 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2211 2527 2893 4294 4481 1700 Size: 110 401 316 366 1401 187 2869 Fragments arranged by size: 2869 1401 401 366 316 187 110 BsrGI T'GTAC_A Cuts at: 96 2925 96 Size: 2829 2821 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5650 BssSI C'ACGA_G Cuts at: 2201 3710 4722 2201 Size: 1509 1012 3129 Fragments arranged by size: 3129 1509 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5650 BstBI TT'CG_AA Cuts at: 2007 4454 2007 Size: 2447 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5650 BstXI CCAn_nnnn'nTGG Cuts at: 2479 2658 2479 Size: 179 5471 BstYI r'GATC_y Cuts at: 1972 2475 2508 2604 2980 3347 3732 3749 5201 Size: 503 33 96 376 367 385 17 1452 Cuts at: 5201 5287 5299 5645 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 503 385 376 367 346 96 86 33 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5098 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4911 Fragments arranged by size: 4911 683 56 BtsI GCAGTG_nn' Cuts at: 2463 3166 3960 3980 2463 Size: 703 794 20 4133 Fragments arranged by size: 4133 794 703 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5650 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5615 DraI TTT'AAA Cuts at: 2954 2954 Size: 5650 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4657 809 Size: 507 3341 1802 Fragments arranged by size: 3341 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2238 2451 2866 Size: 54 1425 50 275 18 408 213 415 Cuts at: 2866 3097 3986 5615 8 Size: 231 889 1629 43 Fragments arranged by size: 1629 1425 889 415 408 275 231 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3097 1537 Size: 275 1285 4090 Fragments arranged by size: 4090 1285 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2336 2583 3580 1353 Size: 983 247 997 3423 Fragments arranged by size: 3423 997 983 247 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2510 4237 4611 4757 387 Size: 1064 138 921 1727 374 146 1280 Fragments arranged by size: 1727 1280 1064 921 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2654 3713 4310 5097 5365 1239 Size: 393 1022 1059 597 787 268 1524 Fragments arranged by size: 1524 1059 1022 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2197 2332 2197 Size: 135 5515 EcoRI G'AATT_C Cuts at: 2248 2959 3337 2248 Size: 711 378 4561 Fragments arranged by size: 4561 711 378 FspI TGC'GCA Cuts at: 4156 4156 Size: 5650 HaeII r_GCGC'y Cuts at: 1678 4797 1678 Size: 3119 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2870 2902 4291 4323 4365 Size: 32 135 32 1298 32 1389 32 42 Cuts at: 4365 4397 1373 Size: 32 2626 Fragments arranged by size: 2626 1389 1298 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2374 2994 3240 669 Size: 988 281 436 620 246 3079 Fragments arranged by size: 3079 988 620 436 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5650 HpaI GTT'AAC Cuts at: 3240 3240 Size: 5650 KpnI G_GTAC'C Cuts at: 2975 2975 Size: 5650 MfeI C'AATT_G Cuts at: 3249 3249 Size: 5650 MluI A'CGCG_T Cuts at: 5526 5526 Size: 5650 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2298 4764 4948 1305 Size: 993 2466 184 2007 Fragments arranged by size: 2466 2007 993 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2453 10 Size: 54 1425 343 621 3207 Fragments arranged by size: 3207 1425 621 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2099 2576 2786 2789 3608 3967 4126 Size: 648 933 477 210 3 819 359 159 Cuts at: 4126 518 Size: 2042 Fragments arranged by size: 2042 933 819 648 477 359 210 159 3 MspA1I CmG'CkG Cuts at: 1606 2236 2923 3066 3743 4891 5136 1606 Size: 630 687 143 677 1148 245 2120 Fragments arranged by size: 2120 1148 687 677 630 245 143 NaeI GCC'GGC Cuts at: 2213 2213 Size: 5650 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5098 NdeI CA'TA_TG Cuts at: 387 387 Size: 5650 NgoMIV G'CCGG_C Cuts at: 2211 2211 Size: 5650 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5650 NotI GC'GGCC_GC Cuts at: 3097 3097 Size: 5650 NspI r_CATG'y Cuts at: 1132 1229 3008 4553 1132 Size: 97 1779 1545 2229 Fragments arranged by size: 2229 1779 1545 97 PasI CC'CwG_GG Cuts at: 1348 2651 1348 Size: 1303 4347 PciI A'CATG_T Cuts at: 4549 4549 Size: 5650 PflMI CCAn_nnn'nTGG Cuts at: 1108 2875 1108 Size: 1767 3883 PmeI GTTT'AAAC Cuts at: 2954 2954 Size: 5650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3719 3751 5232 5264 3719 Size: 32 1481 32 4105 Fragments arranged by size: 4105 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2332 2332 Size: 5650 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5650 PsiI TTA'TAA Cuts at: 3220 3220 Size: 5650 PstI C_TGCA'G Cuts at: 830 2312 2470 2856 3002 830 Size: 1482 158 386 146 3478 Fragments arranged by size: 3478 1482 386 158 146 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5650 PvuII CAG'CTG Cuts at: 2236 2923 2236 Size: 687 4963 SalI G'TCGA_C Cuts at: 1936 2992 1936 Size: 1056 4594 SbfI CC_TGCA'GG Cuts at: 3002 3002 Size: 5650 ScaI AGT'ACT Cuts at: 3898 5610 3898 Size: 1712 3938 SfcI C'TryA_G Cuts at: 826 1045 2308 2466 2852 2998 4133 4814 5005 Size: 219 1263 158 386 146 1135 681 191 Cuts at: 5005 826 Size: 1471 Fragments arranged by size: 1471 1263 1135 681 386 219 191 158 146 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 SgrAI Cr'CCGG_yG Cuts at: 1700 2527 1700 Size: 827 4823 SmaI CCC'GGG Cuts at: 1872 2717 2977 1872 Size: 845 260 4545 Fragments arranged by size: 4545 845 260 SmlI C'TyrA_G Cuts at: 820 1017 1943 3754 4655 4917 820 Size: 197 926 1811 901 262 1553 Fragments arranged by size: 1811 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2947 3441 493 Size: 2454 494 2702 Fragments arranged by size: 2702 2454 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5650 SphI G_CATG'C Cuts at: 1132 3008 1132 Size: 1876 3774 SspI AAT'ATT Cuts at: 5 52 3574 5 Size: 47 3522 2081 Fragments arranged by size: 3522 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5650 StyI C'CwwG_G Cuts at: 513 1065 2273 3089 513 Size: 552 1208 816 3074 Fragments arranged by size: 3074 1208 816 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2042 2751 3677 3694 3847 4032 4477 Size: 1255 462 709 926 17 153 185 445 Cuts at: 4477 325 Size: 1498 Fragments arranged by size: 1498 1255 926 709 462 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2925 3896 5608 Size: 274 80 33 51 1443 948 971 1712 Cuts at: 5608 96 Size: 138 Fragments arranged by size: 1712 1443 971 948 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2500 2532 5610 5642 2500 Size: 32 3078 32 2508 Fragments arranged by size: 3078 2508 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5650 XbaI T'CTAG_A Cuts at: 2986 2986 Size: 5650 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2183 2842 2183 Size: 659 4991 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5650 XmaI C'CCGG_G Cuts at: 1870 2715 2975 1870 Size: 845 260 4545 Fragments arranged by size: 4545 845 260 XmnI GAAnn'nnTTC Cuts at: 3779 3779 Size: 5650 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3458 276 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI