Restriction map for
pFN21ASDA0478
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# NO CUT ENZYMES ( 86 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AgeI AhdI AscI AseI
AsnI AspEI AviII AvrII BalI
BamHI BbeI BciVI BfrI BglI
BlnI BsaBI BsaHI BseAI BsiEI
BsiWI BspEI BssHII Bst1107I BstBI
BstUI BstXI BstZ17I CpoI DrdI
EagI Eam1105I EclXI Eco52I FseI
FspI Hin1I HincII HindII HpaI
KasI KspI MamI MluI MluNI
MroI MscI MvnI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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Aor51HI 2267 AGCGCT AGC^GCT
ApaI 504 GGGCCC GGGCC^C
AspI 1044 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 202 CCCGAG C^[CT]CG[GA]G
BbvCI 1255 GCTGAGG CCTCAGC(-5/-2)
BcgI 204 CGAGGAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2954 AGATCT A^GATCT
BlpI 1887 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1887 GCTGAGC GC^T[ACGT]AGC
BsmBI 1398 GAGACG CGTCTC(1/5)
BsoBI 202 CCCGAG C^[CT]CG[GA]G
BspDI 181 ATCGAT AT^CGAT
BspLU11I 2422 ACATGT A^CATGT
BsrBI 2980 GAGCGG CCGCTC(-3/-3)
BsrDI 3353 CATTGC GCAATG(2/0)
BsrFI 20 GCCGGC [GA]^CCGG[CT]
BssSI 3652 CTCGTG CACGAG(-5/-1)
CelII 1887 GCTGAGC GC^T[ACGT]AGC
Cfr10I 20 GCCGGC [GA]^CCGG[CT]
ClaI 181 ATCGAT AT^CGAT
DraI 783 TTTAAA TTT^AAA
EaeI 1916 CGGCCA [CT]^GGCC[GA]
Eco47III 2267 AGCGCT AGC^GCT
EcoNI 2007 CCTTACTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1551 GATATC GAT^ATC
HaeII 2267 AGCGCT [GA]GCGC^[CT]
MfeI 3049 CAATTG C^AATTG
MunI 3049 CAATTG C^AATTG
NaeI 20 GCCGGC GCC^GGC
NgoMIV 20 GCCGGC G^CCGGC
PflFI 1044 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 2687 AGTACT AGT^ACT
SmlI 431 CTCAAG C^T[CT][GA]AG
SspI 950 AATATT AAT^ATT
Tth111I 1044 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 35 enzymes) #
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Acc65I 91 GGTACC G^GTACC
3065 GGTACC
AccI 296 GTCTAC GT^[AC][GT]AC
1003 GTAGAC
AflIII 2422 ACATGT A^C[GA][CT]GT
2944 ACGTGT
Alw44I 2257 GTGCAC G^TGCAC
2914 GTGCAC
AocI 1734 CCTGAGG CC^T[ACGT]AGG
2456 CCTCAGG
ApaLI 2257 GTGCAC G^TGCAC
2914 GTGCAC
Asp718I 91 GGTACC G^GTACC
3065 GGTACC
BbrPI 2731 CACGTG CAC^GTG
2943 CACGTG
BclI 2127 TGATCA T^GATCA
3566 TGATCA
BsaAI 2731 CACGTG [CT]AC^GT[GA]
2943 CACGTG
BsaWI 2894 ACCGGA [AT]^CCGG[AT]
3311 TCCGGT
Bsp1407I 41 TGTACA T^GTACA
233 TGTACA
BspHI 1407 TCATGA T^CATGA
2609 TCATGA
BsrGI 41 TGTACA T^GTACA
233 TGTACA
BstEII 768 GGTTACC G^GT[ACGT]ACC
2991 GGTGACC
BstPI 768 GGTTACC G^GT[ACGT]ACC
2991 GGTGACC
Bsu36I 1734 CCTGAGG CC^T[ACGT]AGG
2456 CCTCAGG
DraIII 45 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2986 CACATGGTG
Eco81I 1734 CCTGAGG CC^T[ACGT]AGG
2456 CCTCAGG
EcoO65I 768 GGTTACC G^GT[ACGT]ACC
2991 GGTGACC
EcoRI 564 GAATTC G^AATTC
1141 GAATTC
EcoT22I 185 ATGCAT ATGCA^T
2678 ATGCAT
FauI 2726 CCCGC CCCGC(4/6)
3023 GCGGG
FbaI 2127 TGATCA T^GATCA
3566 TGATCA
KpnI 91 GGTACC GGTAC^C
3065 GGTACC
NcoI 1898 CCATGG C^CATGG
2364 CCATGG
NsiI 185 ATGCAT ATGCA^T
2678 ATGCAT
PmaCI 2731 CACGTG CAC^GTG
2943 CACGTG
PmlI 2731 CACGTG CAC^GTG
2943 CACGTG
RcaI 1407 TCATGA T^CATGA
2609 TCATGA
SapI 2199 GAAGAGC GCTCTTC(1/4)
3246 GCTCTTC
SphI 2462 GCATGC GCATG^C
2680 GCATGC
SspBI 41 TGTACA T^GTACA
233 TGTACA
XbaI 732 TCTAGA T^CTAGA
1563 TCTAGA
XcmI 2364 CCATGGTGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3045 CCAGCAATTGTCTGG
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI