Restriction map for
pFN21ASDA0478
(Linear) (Six-base) MAPSORT of: KIAA0478.seq Check: 6046 from: 1 to: 3717
KIAA0478.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2362 3513 3552 3591 3717
Size: 2362 1151 39 39 126
Fragments arranged by size:
2362 1151 126 39 39
AccI GT'mk_AC
Cuts at: 0 297 1004 3717
Size: 297 707 2713
Fragments arranged by size:
2713 707 297
Acc65I G'GTAC_C
Cuts at: 0 91 3065 3717
Size: 91 2974 652
Fragments arranged by size:
2974 652 91
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 473 650 1256 1886 2159 2572 2869
Size: 87 386 177 606 630 273 413 297
Cuts at: 2869 2943 3000 3717
Size: 74 57 717
Fragments arranged by size:
717 630 606 413 386 297 273 177
87 74 57
AfeI AGC'GCT
Cuts at: 0 2269 3717
Size: 2269 1448
AflIII A'CryG_T
Cuts at: 0 2422 2944 3717
Size: 2422 522 773
Fragments arranged by size:
2422 773 522
AleI CACnn'nnGTG
Cuts at: 0 3138 3717
Size: 3138 579
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2311 3717
Size: 2311 1406
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 49 81 2572 2604 3717
Size: 49 32 2491 32 1113
Fragments arranged by size:
2491 1113 49 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 30 39 1822 2191 2774 3149 3717
Size: 30 9 1783 369 583 375 568
Fragments arranged by size:
1783 583 568 375 369 30 9
ApaI G_GGCC'C
Cuts at: 0 508 3717
Size: 508 3209
ApaLI G'TGCA_C
Cuts at: 0 2257 2914 3717
Size: 2257 657 803
Fragments arranged by size:
2257 803 657
ApoI r'AATT_y
Cuts at: 0 564 723 1141 1215 2053 2443 3717
Size: 564 159 418 74 838 390 1274
Fragments arranged by size:
1274 838 564 418 390 159 74
AvaI C'yCGr_G
Cuts at: 0 202 3717
Size: 202 3515
BanI G'GyrC_C
Cuts at: 0 91 1675 3065 3717
Size: 91 1584 1390 652
Fragments arranged by size:
1584 1390 652 91
BanII G_rGCy'C
Cuts at: 0 8 111 202 508 2384 3248 3416 3545
Size: 8 103 91 306 1876 864 168 129
Cuts at: 3545 3653 3717
Size: 108 64
Fragments arranged by size:
1876 864 306 168 129 108 103 91
64 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 685 741 1249 1294 2108 3717
Size: 685 56 508 45 814 1609
Fragments arranged by size:
1609 814 685 508 56 45
BbvCI CC'TCA_GC
Cuts at: 0 1256 3717
Size: 1256 2461
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 193 227 3717
Size: 193 34 3490
Fragments arranged by size:
3490 193 34
BclI T'GATC_A
Cuts at: 0 2127 3566 3717
Size: 2127 1439 151
Fragments arranged by size:
2127 1439 151
BfrBI ATG'CAT
Cuts at: 0 187 2680 3717
Size: 187 2493 1037
Fragments arranged by size:
2493 1037 187
BglII A'GATC_T
Cuts at: 0 2954 3717
Size: 2954 763
BlpI GC'TnA_GC
Cuts at: 0 1888 3717
Size: 1888 1829
Bme1580I G_kGCm'C
Cuts at: 0 508 2261 2918 2967 3717
Size: 508 1753 657 49 750
Fragments arranged by size:
1753 750 657 508 49
BmrI ACTGGGnnnn_n'
Cuts at: 0 2703 3717
Size: 2703 1014
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2934 3717
Size: 2934 783
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 1261 1505 2377 2998 3121 3368 3717
Size: 482 779 244 872 621 123 247 349
Fragments arranged by size:
872 779 621 482 349 247 244 123
Bpu10I CC'TnA_GC
Cuts at: 0 285 1256 3717
Size: 285 971 2461
Fragments arranged by size:
2461 971 285
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 3717
Size: 416 3301
BsaI GGTCTCn'nnnn_
Cuts at: 0 1098 2311 3388 3574 3717
Size: 1098 1213 1077 186 143
Fragments arranged by size:
1213 1098 1077 186 143
BsaAI yAC'GTr
Cuts at: 0 2733 2945 3717
Size: 2733 212 772
Fragments arranged by size:
2733 772 212
BsaWI w'CCGG_w
Cuts at: 0 2894 3311 3717
Size: 2894 417 406
Fragments arranged by size:
2894 417 406
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 49 79 253 283 3717
Size: 49 30 174 30 3434
Fragments arranged by size:
3434 174 49 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 220 1048 1264 1816 1819 3592 3717
Size: 220 828 216 552 3 1773 125
Fragments arranged by size:
1773 828 552 220 216 125 3
BseYI C'CCAG_C
Cuts at: 0 606 3302 3443 3584 3717
Size: 606 2696 141 141 133
Fragments arranged by size:
2696 606 141 141 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1962 2317 2331 2390 2803 2883 3162
Size: 1033 929 355 14 59 413 80 279
Cuts at: 3162 3717
Size: 555
Fragments arranged by size:
1033 929 555 413 355 279 80 59
14
BsiHKAI G_wGCw'C
Cuts at: 0 8 1030 1811 2261 2489 2918 3182 3248
Size: 8 1022 781 450 228 429 264 66
Cuts at: 3248 3380 3416 3473 3490 3653 3717
Size: 132 36 57 17 163 64
Fragments arranged by size:
1022 781 450 429 264 228 163 132
66 64 57 36 17 8
BsmI GAATG_Cn'
Cuts at: 0 600 871 1841 3291 3717
Size: 600 271 970 1450 426
Fragments arranged by size:
1450 970 600 426 271
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1392 3717
Size: 1392 2325
Bsp1286I G_dGCh'C
Cuts at: 0 8 111 202 508 1030 1811 2261 2384
Size: 8 103 91 306 522 781 450 123
Cuts at: 2384 2489 2918 2967 3182 3248 3380 3416 3473
Size: 105 429 49 215 66 132 36 57
Cuts at: 3473 3490 3545 3653 3717
Size: 17 55 108 64
Fragments arranged by size:
781 522 450 429 306 215 132 123
108 105 103 91 66 64 57 55
49 36 17 8
BspHI T'CATG_A
Cuts at: 0 1407 2609 3717
Size: 1407 1202 1108
Fragments arranged by size:
1407 1202 1108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1652 2362 3261 3513 3552 3591 3717
Size: 1652 710 899 252 39 39 126
Fragments arranged by size:
1652 899 710 252 126 39 39
BsrBI CCG'CTC
Cuts at: 0 2982 3717
Size: 2982 735
BsrDI GCAATG_nn'
Cuts at: 0 3352 3717
Size: 3352 365
BsrFI r'CCGG_y
Cuts at: 0 20 3717
Size: 20 3697
BsrGI T'GTAC_A
Cuts at: 0 41 233 3717
Size: 41 192 3484
Fragments arranged by size:
3484 192 41
BssSI C'ACGA_G
Cuts at: 0 3652 3717
Size: 3652 65
BstAPI GCAn_nnn'nTGC
Cuts at: 0 30 1605 2603 2861 3205 3717
Size: 30 1575 998 258 344 512
Fragments arranged by size:
1575 998 512 344 258 30
BstEII G'GTnAC_C
Cuts at: 0 768 2991 3717
Size: 768 2223 726
Fragments arranged by size:
2223 768 726
BstYI r'GATC_y
Cuts at: 0 2841 2954 3026 3717
Size: 2841 113 72 691
Fragments arranged by size:
2841 691 113 72
Bsu36I CC'TnA_GG
Cuts at: 0 1735 2457 3717
Size: 1735 722 1260
Fragments arranged by size:
1735 1260 722
BtgI C'CryG_G
Cuts at: 0 1898 2364 3371 3545 3717
Size: 1898 466 1007 174 172
Fragments arranged by size:
1898 1007 466 174 172
BtsI GCAGTG_nn'
Cuts at: 0 1703 1889 1950 2305 2319 2601 3717
Size: 1703 186 61 355 14 282 1116
Fragments arranged by size:
1703 1116 355 282 186 61 14
ClaI AT'CG_AT
Cuts at: 0 182 3717
Size: 182 3535
DraI TTT'AAA
Cuts at: 0 785 3717
Size: 785 2932
DraIII CAC_nnn'GTG
Cuts at: 0 50 2991 3717
Size: 50 2941 726
Fragments arranged by size:
2941 726 50
EaeI y'GGCC_r
Cuts at: 0 1916 3717
Size: 1916 1801
EarI CTCTTCn'nnn_
Cuts at: 0 1232 2194 2401 2510 2888 3253 3433 3717
Size: 1232 962 207 109 378 365 180 284
Fragments arranged by size:
1232 962 378 365 284 207 180 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2619 3717
Size: 2619 1098
EcoICRI GAG'CTC
Cuts at: 0 6 3246 3414 3651 3717
Size: 6 3240 168 237 66
Fragments arranged by size:
3240 237 168 66 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 473 482 650 1256 1261 1505 1886
Size: 87 386 9 168 606 5 244 381
Cuts at: 1886 2159 2377 2572 2869 2943 2998 3000 3121
Size: 273 218 195 297 74 55 2 121
Cuts at: 3121 3368 3717
Size: 247 349
Fragments arranged by size:
606 386 381 349 297 273 247 244
218 195 168 121 87 74 55 9
5 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2011 3717
Size: 2011 1706
EcoO109I rG'GnC_Cy
Cuts at: 0 504 505 1077 2738 3299 3717
Size: 504 1 572 1661 561 418
Fragments arranged by size:
1661 572 561 504 418 1
EcoRI G'AATT_C
Cuts at: 0 564 1141 3717
Size: 564 577 2576
Fragments arranged by size:
2576 577 564
EcoRV GAT'ATC
Cuts at: 0 1553 3717
Size: 1553 2164
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 282 2220 3264 3717
Size: 282 1938 1044 453
Fragments arranged by size:
1938 1044 453 282
HaeII r_GCGC'y
Cuts at: 0 2271 3717
Size: 2271 1446
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 1389 1421 3717
Size: 160 32 1197 32 2296
Fragments arranged by size:
2296 1197 160 32 32
HindIII A'AGCT_T
Cuts at: 0 115 632 1507 1868 2229 3717
Size: 115 517 875 361 361 1488
Fragments arranged by size:
1488 875 517 361 361 115
KpnI G_GTAC'C
Cuts at: 0 95 3069 3717
Size: 95 2974 648
Fragments arranged by size:
2974 648 95
MfeI C'AATT_G
Cuts at: 0 3049 3717
Size: 3049 668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 1386 1791 2982 3717
Size: 203 1183 405 1191 735
Fragments arranged by size:
1191 1183 735 405 203
MslI CAynn'nnrTG
Cuts at: 0 2492 2906 3138 3227 3312 3717
Size: 2492 414 232 89 85 405
Fragments arranged by size:
2492 414 405 232 89 85
MspA1I CmG'CkG
Cuts at: 0 27 39 610 628 720 1101 2355 3020
Size: 27 12 571 18 92 381 1254 665
Cuts at: 3020 3717
Size: 697
Fragments arranged by size:
1254 697 665 571 381 92 27 18
12
NaeI GCC'GGC
Cuts at: 0 22 3717
Size: 22 3695
NcoI C'CATG_G
Cuts at: 0 1898 2364 3717
Size: 1898 466 1353
Fragments arranged by size:
1898 1353 466
NgoMIV G'CCGG_C
Cuts at: 0 20 3717
Size: 20 3697
NsiI A_TGCA'T
Cuts at: 0 189 2682 3717
Size: 189 2493 1035
Fragments arranged by size:
2493 1035 189
NspI r_CATG'y
Cuts at: 0 1717 2426 2466 2684 3208 3717
Size: 1717 709 40 218 524 509
Fragments arranged by size:
1717 709 524 509 218 40
PciI A'CATG_T
Cuts at: 0 2422 3717
Size: 2422 1295
PfoI T'CCnGG_A
Cuts at: 0 3345 3717
Size: 3345 372
PmlI CAC'GTG
Cuts at: 0 2733 2945 3717
Size: 2733 212 772
Fragments arranged by size:
2733 772 212
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 49 81 3717
Size: 49 32 3636
Fragments arranged by size:
3636 49 32
PpuMI rG'GwC_Cy
Cuts at: 0 1077 2738 3299 3717
Size: 1077 1661 561 418
Fragments arranged by size:
1661 1077 561 418
PspOMI G'GGCC_C
Cuts at: 0 504 3717
Size: 504 3213
PstI C_TGCA'G
Cuts at: 0 35 966 1973 2770 3272 3717
Size: 35 931 1007 797 502 445
Fragments arranged by size:
1007 931 797 502 445 35
PvuII CAG'CTG
Cuts at: 0 27 39 628 720 1101 2355 3020 3717
Size: 27 12 589 92 381 1254 665 697
Fragments arranged by size:
1254 697 665 589 381 92 27 12
SacI G_AGCT'C
Cuts at: 0 8 3248 3416 3653 3717
Size: 8 3240 168 237 64
Fragments arranged by size:
3240 237 168 64 8
SanDI GG'GwC_CC
Cuts at: 0 3299 3717
Size: 3299 418
SapI GCTCTTCn'nnn_
Cuts at: 0 2194 3253 3717
Size: 2194 1059 464
Fragments arranged by size:
2194 1059 464
ScaI AGT'ACT
Cuts at: 0 2689 3717
Size: 2689 1028
SfcI C'TryA_G
Cuts at: 0 15 31 298 623 962 1969 2766 3268
Size: 15 16 267 325 339 1007 797 502
Cuts at: 3268 3717
Size: 449
Fragments arranged by size:
1007 797 502 449 339 325 267 16
15
SmlI C'TyrA_G
Cuts at: 0 431 3717
Size: 431 3286
SphI G_CATG'C
Cuts at: 0 2466 2684 3717
Size: 2466 218 1033
Fragments arranged by size:
2466 1033 218
SspI AAT'ATT
Cuts at: 0 952 3717
Size: 952 2765
StuI AGG'CCT
Cuts at: 0 1967 2218 2626 3040 3717
Size: 1967 251 408 414 677
Fragments arranged by size:
1967 677 414 408 251
StyI C'CwwG_G
Cuts at: 0 1133 1199 1898 2321 2364 3717
Size: 1133 66 699 423 43 1353
Fragments arranged by size:
1353 1133 699 423 66 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1212 2635 3488 3717
Size: 1212 1423 853 229
Fragments arranged by size:
1423 1212 853 229
TatI w'GTAC_w
Cuts at: 0 41 233 2687 3320 3717
Size: 41 192 2454 633 397
Fragments arranged by size:
2454 633 397 192 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 253 285 3103 3135 3717
Size: 253 32 2818 32 582
Fragments arranged by size:
2818 582 253 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1047 3717
Size: 1047 2670
XbaI T'CTAG_A
Cuts at: 0 732 1563 3717
Size: 732 831 2154
Fragments arranged by size:
2154 831 732
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2371 3052 3717
Size: 2371 681 665
Fragments arranged by size:
2371 681 665
XmnI GAAnn'nnTTC
Cuts at: 0 844 2203 2503 2956 3013 3717
Size: 844 1359 300 453 57 704
Fragments arranged by size:
1359 844 704 453 300 57
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII
BbsI BbvCI BcgI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstYI Bsu36I BtgI BtsI ClaI DraI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HindIII KpnI MfeI MmeI
MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PciI
PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI
SanDI SapI ScaI SfcI SmlI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BciVI BglI BmgBI BmtI
BsaBI BsaHI BsiEI BsiWI BspEI BssHII BstBI BstXI
BstZ17I BtgZI CspCI DrdI EagI FseI FspI FspAI
HincII HpaI KasI MluI MscI NarI NdeI NheI
NotI NruI PacI PasI PflMI PmeI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SwaI XhoI XmaI ZraI