(Circular) (Six-base) MAPSORT of: pFN21ASDA0475.seq Check: 1066 from: 1 to: 5956 pFN21ASDA0475 5956 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3766 278 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3299 1937 Size: 1362 4594 Acc65I G'GTAC_C Cuts at: 3277 3277 Size: 5956 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2277 4019 5403 5671 1632 Size: 645 1742 1384 268 1917 Fragments arranged by size: 1917 1742 1384 645 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5759 AflIII A'CryG_T Cuts at: 1203 4855 5832 1203 Size: 3652 977 1327 Fragments arranged by size: 3652 1327 977 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 4685 4685 Size: 5956 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5956 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5956 AlwNI CAG_nnn'CTG Cuts at: 3699 5271 3699 Size: 1572 4384 ApaLI G'TGCA_C Cuts at: 3187 4013 5169 3187 Size: 826 1156 3974 Fragments arranged by size: 3974 1156 826 ApoI r'AATT_y Cuts at: 1493 3265 3491 3643 5752 1493 Size: 1772 226 152 2109 1697 Fragments arranged by size: 2109 1772 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4510 160 Size: 872 3478 1606 Fragments arranged by size: 3478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 2241 3281 1870 Size: 73 298 1040 4545 Fragments arranged by size: 4545 1040 298 73 BamHI G'GATC_C Cuts at: 3286 3653 3286 Size: 367 5589 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2179 3277 4732 618 Size: 325 217 627 392 1098 1455 1842 Fragments arranged by size: 1842 1455 1098 627 392 325 217 BanII G_rGCy'C Cuts at: 1391 2509 3275 1391 Size: 1118 766 4072 Fragments arranged by size: 4072 1118 766 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3231 928 Size: 971 1332 3653 Fragments arranged by size: 3653 1332 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4147 4181 1903 Size: 34 2210 34 3678 Fragments arranged by size: 3678 2210 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3848 5058 3848 Size: 1210 4746 BclI T'GATC_A Cuts at: 1548 1548 Size: 5956 BfrBI ATG'CAT Cuts at: 2287 2287 Size: 5956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4567 136 Size: 107 122 71 631 605 2895 1525 Fragments arranged by size: 2895 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5951 5951 Size: 5956 BlpI GC'TnA_GC Cuts at: 3373 3373 Size: 5956 Bme1580I G_kGCm'C Cuts at: 1790 3191 4017 5173 1790 Size: 1401 826 1156 2573 Fragments arranged by size: 2573 1401 1156 826 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5956 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2221 3110 3687 Size: 416 53 83 399 24 804 889 577 Cuts at: 3687 4645 442 Size: 958 1753 Fragments arranged by size: 1753 958 889 804 577 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4616 1239 Size: 3377 2579 Bpu10I CC'TnA_GC Cuts at: 2394 2394 Size: 5956 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4081 4946 5244 4081 Size: 865 298 4793 Fragments arranged by size: 4793 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2403 4619 882 Size: 617 904 2216 2219 Fragments arranged by size: 2219 2216 904 617 BsaAI yAC'GTr Cuts at: 493 1204 3253 3747 493 Size: 711 2049 494 2702 Fragments arranged by size: 2702 2049 711 494 BsaBI GATnn'nnATC Cuts at: 3132 5950 3132 Size: 2818 3138 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3141 3763 4145 4780 Size: 53 83 186 936 1608 622 382 635 Cuts at: 4780 275 Size: 1451 Fragments arranged by size: 1608 1451 936 635 622 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4389 4787 5061 5208 5619 4389 Size: 398 274 147 411 4726 Fragments arranged by size: 4726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3095 3125 1178 Size: 30 165 30 1692 30 4009 Fragments arranged by size: 4009 1692 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5956 BseYI C'CCAG_C Cuts at: 2272 5159 5655 2272 Size: 2887 496 2573 Fragments arranged by size: 2887 2573 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5383 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3406 4167 5195 1540 Size: 275 186 1405 761 1028 2301 Fragments arranged by size: 2301 1405 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2582 3123 3191 4017 4102 5173 2582 Size: 541 68 826 85 1071 3365 Fragments arranged by size: 3365 1071 826 541 85 68 BsmI GAATG_Cn' Cuts at: 2991 3467 3560 2991 Size: 476 93 5387 Fragments arranged by size: 5387 476 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2509 2582 3123 3191 3275 4017 4102 Size: 399 719 73 541 68 84 742 85 Cuts at: 4102 5173 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 719 541 399 85 84 73 68 BspEI T'CCGG_A Cuts at: 5619 5619 Size: 5956 BspHI T'CATG_A Cuts at: 3845 5575 3845 Size: 1730 4226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3311 844 Size: 2467 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3843 1394 Size: 429 2020 3507 Fragments arranged by size: 3507 2020 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2887 4451 4625 65 Size: 1183 1639 1564 174 1396 Fragments arranged by size: 1639 1564 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4600 4787 1700 Size: 110 2790 187 2869 Fragments arranged by size: 2869 2790 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5956 BssSI C'ACGA_G Cuts at: 4016 5028 4016 Size: 1012 4944 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5956 BstBI TT'CG_AA Cuts at: 2007 4760 2007 Size: 2753 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5956 BstXI CCAn_nnnn'nTGG Cuts at: 2706 2706 Size: 5956 BstYI r'GATC_y Cuts at: 1972 2678 3286 3653 4038 4055 5507 5593 5605 Size: 706 608 367 385 17 1452 86 12 Cuts at: 5605 5951 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 706 608 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2292 2732 3093 3144 513 Size: 552 1227 440 361 51 3325 Fragments arranged by size: 3325 1227 552 440 361 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5217 Fragments arranged by size: 5217 683 56 BtsI GCAGTG_nn' Cuts at: 3191 3472 4266 4286 3191 Size: 281 794 20 4861 Fragments arranged by size: 4861 794 281 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5921 DraI TTT'AAA Cuts at: 3260 3260 Size: 5956 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2858 4963 809 Size: 507 1542 2105 1802 Fragments arranged by size: 2105 1802 1542 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2295 2763 3171 Size: 54 1425 50 275 18 465 468 408 Cuts at: 3171 3403 4292 5921 8 Size: 232 889 1629 43 Fragments arranged by size: 1629 1425 889 468 465 408 275 232 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3403 1537 Size: 275 1591 4090 Fragments arranged by size: 4090 1591 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2253 3886 1353 Size: 900 1633 3423 Fragments arranged by size: 3423 1633 900 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4543 4917 5063 387 Size: 1064 138 2954 374 146 1280 Fragments arranged by size: 2954 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2277 4019 4616 5403 5671 1239 Size: 393 645 1742 597 787 268 1524 Fragments arranged by size: 1742 1524 787 645 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2139 2139 Size: 5956 EcoRI G'AATT_C Cuts at: 3265 3643 3265 Size: 378 5578 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3097 3097 Size: 5956 FspI TGC'GCA Cuts at: 4462 4462 Size: 5956 HaeII r_GCGC'y Cuts at: 1678 2928 5103 1678 Size: 1250 2175 2531 Fragments arranged by size: 2531 2175 1250 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3095 3127 4597 4629 4671 Size: 32 135 32 1523 32 1470 32 42 Cuts at: 4671 4703 1373 Size: 32 2626 Fragments arranged by size: 2626 1523 1470 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3300 3546 669 Size: 988 281 1362 246 3079 Fragments arranged by size: 3079 1362 988 281 246 HindIII A'AGCT_T Cuts at: 748 2959 748 Size: 2211 3745 HpaI GTT'AAC Cuts at: 3546 3546 Size: 5956 KpnI G_GTAC'C Cuts at: 3281 3281 Size: 5956 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5070 5254 1305 Size: 3765 184 2007 Fragments arranged by size: 3765 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2297 2765 3173 10 Size: 54 1425 343 465 468 408 2793 Fragments arranged by size: 2793 1425 468 465 408 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2898 3026 3914 4273 4432 518 Size: 648 1732 128 888 359 159 2042 Fragments arranged by size: 2042 1732 888 648 359 159 128 MspA1I CmG'CkG Cuts at: 1606 2117 2344 2572 2858 3154 3372 4049 5197 Size: 511 227 228 286 296 218 677 1148 Cuts at: 5197 5442 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 511 296 286 245 228 227 218 NcoI C'CATG_G Cuts at: 513 1065 2292 2732 3093 513 Size: 552 1227 440 361 3376 Fragments arranged by size: 3376 1227 552 440 361 NdeI CA'TA_TG Cuts at: 387 387 Size: 5956 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 3403 3403 Size: 5956 NsiI A_TGCA'T Cuts at: 2289 2289 Size: 5956 NspI r_CATG'y Cuts at: 1132 1229 2291 3314 4859 1132 Size: 97 1062 1023 1545 2229 Fragments arranged by size: 2229 1545 1062 1023 97 PasI CC'CwG_GG Cuts at: 1348 2211 1348 Size: 863 5093 PciI A'CATG_T Cuts at: 4855 4855 Size: 5956 PflMI CCAn_nnn'nTGG Cuts at: 1108 2233 2419 1108 Size: 1125 186 4645 Fragments arranged by size: 4645 1125 186 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4025 4057 5538 5570 4025 Size: 32 1481 32 4411 Fragments arranged by size: 4411 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5956 PsiI TTA'TAA Cuts at: 2331 3526 2331 Size: 1195 4761 PstI C_TGCA'G Cuts at: 830 2718 3308 830 Size: 1888 590 3478 Fragments arranged by size: 3478 1888 590 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 PvuII CAG'CTG Cuts at: 2117 2344 2572 2858 2117 Size: 227 228 286 5215 Fragments arranged by size: 5215 286 228 227 SalI G'TCGA_C Cuts at: 1936 3298 1936 Size: 1362 4594 SbfI CC_TGCA'GG Cuts at: 3308 3308 Size: 5956 ScaI AGT'ACT Cuts at: 3216 4204 5916 3216 Size: 988 1712 3256 Fragments arranged by size: 3256 1712 988 SfcI C'TryA_G Cuts at: 826 1045 2592 2714 3304 4439 5120 5311 826 Size: 219 1547 122 590 1135 681 191 1471 Fragments arranged by size: 1547 1471 1135 681 590 219 191 122 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 2243 3283 1872 Size: 371 1040 4545 Fragments arranged by size: 4545 1040 371 SmlI C'TyrA_G Cuts at: 820 1017 1943 4060 4961 5223 820 Size: 197 926 2117 901 262 1553 Fragments arranged by size: 2117 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3253 3747 493 Size: 2760 494 2702 Fragments arranged by size: 2760 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 2291 3314 1132 Size: 1159 1023 3774 Fragments arranged by size: 3774 1159 1023 SspI AAT'ATT Cuts at: 5 52 3880 5 Size: 47 3828 2081 Fragments arranged by size: 3828 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5956 StyI C'CwwG_G Cuts at: 513 1065 2292 2511 2732 3093 3395 513 Size: 552 1227 219 221 361 302 3074 Fragments arranged by size: 3074 1227 552 361 302 221 219 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2197 2221 2745 3983 4000 4153 4338 Size: 1255 617 24 524 1238 17 153 185 Cuts at: 4338 4783 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1238 617 524 445 185 153 24 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3214 4202 5914 Size: 274 80 33 51 1443 1237 988 1712 Cuts at: 5914 96 Size: 138 Fragments arranged by size: 1712 1443 1237 988 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5916 5948 5916 Size: 32 5924 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5956 XbaI T'CTAG_A Cuts at: 3292 3292 Size: 5956 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5956 XmaI C'CCGG_G Cuts at: 1870 2241 3281 1870 Size: 371 1040 4545 Fragments arranged by size: 4545 1040 371 XmnI GAAnn'nnTTC Cuts at: 4085 4085 Size: 5956 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3764 276 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI