(Circular) (Six-base) MAPSORT of: pFN21ASDA0463.seq Check: 209 from: 1 to: 10456 pFN21ASDA0463 10456 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3871 4136 4548 4741 5363 3871 Size: 265 412 193 622 8964 Fragments arranged by size: 8964 622 412 265 193 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3240 6917 8266 278 Size: 53 83 186 936 1704 3677 1349 2468 Fragments arranged by size: 3677 2468 1704 1349 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2826 3608 5028 5139 6947 7799 1937 Size: 889 782 1420 111 1808 852 4594 Fragments arranged by size: 4594 1808 1420 889 852 782 111 Acc65I G'GTAC_C Cuts at: 7777 7777 Size: 10456 AclI AA'CG_TT Cuts at: 1563 8583 8956 1563 Size: 7020 373 3063 Fragments arranged by size: 7020 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2739 5579 6143 6925 8519 9903 10171 1632 Size: 1107 2840 564 782 1594 1384 268 1917 Fragments arranged by size: 2840 1917 1594 1384 1107 782 564 268 AfeI AGC'GCT Cuts at: 3545 7189 3545 Size: 3644 6812 AflII C'TTAA_G Cuts at: 820 1017 4232 820 Size: 197 3215 7044 Fragments arranged by size: 7044 3215 197 AflIII A'CryG_T Cuts at: 1203 2946 3672 7212 9355 10332 1203 Size: 1743 726 3540 2143 977 1327 Fragments arranged by size: 3540 2143 1743 1327 977 726 AgeI A'CCGG_T Cuts at: 9287 9287 Size: 10456 AhdI GACnn_n'nnGTC Cuts at: 2341 2689 9185 2341 Size: 348 6496 3612 Fragments arranged by size: 6496 3612 348 AleI CACnn'nnGTG Cuts at: 1166 2627 4919 4979 7265 1166 Size: 1461 2292 60 2286 4357 Fragments arranged by size: 4357 2292 2286 1461 60 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2399 2501 1184 Size: 1215 102 9139 Fragments arranged by size: 9139 1215 102 AlwNI CAG_nnn'CTG Cuts at: 3304 3782 4456 4599 6362 7702 8199 9771 3304 Size: 478 674 143 1763 1340 497 1572 3989 Fragments arranged by size: 3989 1763 1572 1340 674 497 478 143 ApaI G_GGCC'C Cuts at: 2098 3921 6485 6982 7669 2098 Size: 1823 2564 497 687 4885 Fragments arranged by size: 4885 2564 1823 687 497 ApaLI G'TGCA_C Cuts at: 3244 4561 7175 8513 9669 3244 Size: 1317 2614 1338 1156 4031 Fragments arranged by size: 4031 2614 1338 1317 1156 ApoI r'AATT_y Cuts at: 1493 5297 7765 7991 8143 10252 1493 Size: 3804 2468 226 152 2109 1697 Fragments arranged by size: 3804 2468 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7728 9010 160 Size: 872 6696 1282 1606 Fragments arranged by size: 6696 1606 1282 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10456 AvaI C'yCGr_G Cuts at: 1870 1943 2091 2679 2846 2970 4961 5190 5859 Size: 73 148 588 167 124 1991 229 669 Cuts at: 5859 6046 6750 6975 7781 1870 Size: 187 704 225 806 4545 Fragments arranged by size: 4545 1991 806 704 669 588 229 225 187 167 148 124 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 6004 6037 6004 Size: 33 10423 BamHI G'GATC_C Cuts at: 5286 7786 8153 5286 Size: 2500 367 7589 Fragments arranged by size: 7589 2500 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2564 3174 3253 4180 4762 Size: 325 217 627 777 610 79 927 582 Cuts at: 4762 5362 6732 7124 7777 9232 618 Size: 600 1370 392 653 1455 1842 Fragments arranged by size: 1842 1455 1370 927 777 653 627 610 600 582 392 325 217 79 BanII G_rGCy'C Cuts at: 1391 2098 3216 3635 3921 5149 5955 6485 6982 Size: 707 1118 419 286 1228 806 530 497 Cuts at: 6982 7659 7669 7775 1391 Size: 677 10 106 4072 Fragments arranged by size: 4072 1228 1118 806 707 677 530 497 419 286 106 10 BbeI G_GCGC'C Cuts at: 3178 3257 6736 3178 Size: 79 3479 6898 Fragments arranged by size: 6898 3479 79 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 9485 BbvCI CC'TCA_GC Cuts at: 2695 3977 2695 Size: 1282 9174 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3107 3141 8647 8681 1903 Size: 34 1170 34 5506 34 3678 Fragments arranged by size: 5506 3678 1170 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 8348 9558 8348 Size: 1210 9246 BclI T'GATC_A Cuts at: 1548 3538 6987 1548 Size: 1990 3449 5017 Fragments arranged by size: 5017 3449 1990 BfrBI ATG'CAT Cuts at: 6823 6823 Size: 10456 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4272 6252 9067 Size: 107 122 71 631 605 2600 1980 2815 Cuts at: 9067 136 Size: 1525 Fragments arranged by size: 2815 2600 1980 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4194 7092 7623 10451 4194 Size: 2898 531 2828 4199 Fragments arranged by size: 4199 2898 2828 531 BlpI GC'TnA_GC Cuts at: 3800 4800 5510 5949 7873 3800 Size: 1000 710 439 1924 6383 Fragments arranged by size: 6383 1924 1000 710 439 Bme1580I G_kGCm'C Cuts at: 1790 2098 2567 3248 3664 3921 4042 4185 4565 Size: 308 469 681 416 257 121 143 380 Cuts at: 4565 4767 6485 6690 6982 7127 7179 7615 7669 Size: 202 1718 205 292 145 52 436 54 Cuts at: 7669 8517 9673 1790 Size: 848 1156 2573 Fragments arranged by size: 2573 1718 1156 848 681 469 436 416 380 308 292 257 205 202 145 143 121 54 52 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10456 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6608 7327 8187 Size: 416 53 83 399 24 5191 719 860 Cuts at: 8187 9145 442 Size: 958 1753 Fragments arranged by size: 5191 1753 958 860 719 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10456 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3502 3837 7645 3502 Size: 335 3808 6313 Fragments arranged by size: 6313 3808 335 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2120 2901 3230 3303 3986 4324 5541 5610 Size: 881 781 329 73 683 338 1217 69 Cuts at: 5610 5930 5939 6101 6203 6338 9116 1239 Size: 320 9 162 102 135 2778 2579 Fragments arranged by size: 2778 2579 1217 881 781 683 338 329 320 162 135 102 73 69 9 Bpu10I CC'TnA_GC Cuts at: 2695 3629 3977 5995 6521 2695 Size: 934 348 2018 526 6630 Fragments arranged by size: 6630 2018 934 526 348 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3479 4496 5864 6266 8581 9446 9744 3479 Size: 1017 1368 402 2315 865 298 4191 Fragments arranged by size: 4191 2315 1368 1017 865 402 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2676 2779 2843 2877 3490 5621 5648 Size: 617 1177 103 64 34 613 2131 27 Cuts at: 5648 7151 9119 882 Size: 1503 1968 2219 Fragments arranged by size: 2219 2131 1968 1503 1177 617 613 103 64 34 27 BsaAI yAC'GTr Cuts at: 493 1204 2947 5713 6109 7753 8247 493 Size: 711 1743 2766 396 1644 494 2702 Fragments arranged by size: 2766 2702 1743 1644 711 494 396 BsaBI GATnn'nnATC Cuts at: 10450 10450 Size: 10456 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3175 3237 3254 6733 Size: 53 83 186 936 1642 62 17 3479 Cuts at: 6733 6914 7371 8263 8645 9280 275 Size: 181 457 892 382 635 1451 Fragments arranged by size: 3479 1642 1451 936 892 635 457 382 186 181 83 62 53 17 BsaWI w'CCGG_w Cuts at: 4521 6348 8889 9287 9561 9708 10119 4521 Size: 1827 2541 398 274 147 411 4858 Fragments arranged by size: 4858 2541 1827 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3662 3692 7433 7463 1178 Size: 30 165 30 2259 30 3741 30 4171 Fragments arranged by size: 4171 3741 2259 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 2725 4205 5801 5804 6865 6925 7667 Size: 850 689 1480 1596 3 1061 60 742 Cuts at: 7667 1186 Size: 3975 Fragments arranged by size: 3975 1596 1480 1061 850 742 689 60 3 BseYI C'CCAG_C Cuts at: 2158 3226 3764 4173 4251 4485 4757 5009 5658 Size: 1068 538 409 78 234 272 252 649 Cuts at: 5658 6135 6696 7432 7481 7494 7531 9659 10155 Size: 477 561 736 49 13 37 2128 496 Cuts at: 10155 2158 Size: 2459 Fragments arranged by size: 2459 2128 1068 736 649 561 538 496 477 409 272 252 234 78 49 37 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2387 2950 3650 4093 4500 4564 4753 Size: 573 651 563 700 443 407 64 189 Cuts at: 4753 4769 6389 7568 1163 Size: 16 1620 1179 4051 Fragments arranged by size: 4051 1620 1179 700 651 573 563 443 407 189 64 16 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4522 5143 5799 6214 6704 7906 Size: 275 186 2521 621 656 415 490 1202 Cuts at: 7906 8667 9695 1540 Size: 761 1028 2301 Fragments arranged by size: 2521 2301 1202 1028 761 656 621 490 415 275 186 BsiHKAI G_wGCw'C Cuts at: 2030 2388 2538 3216 3248 3495 3635 3680 3789 Size: 358 150 678 32 247 140 45 109 Cuts at: 3789 4565 4634 5619 5955 7179 7428 7659 8517 Size: 776 69 985 336 1224 249 231 858 Cuts at: 8517 8602 9673 2030 Size: 85 1071 2813 Fragments arranged by size: 2813 1224 1071 985 858 776 678 358 336 249 247 231 150 140 109 85 69 45 32 BsmI GAATG_Cn' Cuts at: 7561 7967 8060 7561 Size: 406 93 9957 Fragments arranged by size: 9957 406 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2048 2048 Size: 10456 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2098 2388 2538 2567 3216 3248 Size: 399 240 68 290 150 29 649 32 Cuts at: 3248 3495 3635 3664 3680 3789 3921 4042 4185 Size: 247 140 29 16 109 132 121 143 Cuts at: 4185 4565 4634 4767 5149 5619 5955 6485 6690 Size: 380 69 133 382 470 336 530 205 Cuts at: 6690 6982 7127 7179 7428 7615 7659 7669 7775 Size: 292 145 52 249 187 44 10 106 Cuts at: 7775 8517 8602 9673 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 649 530 470 399 382 380 336 292 290 249 247 240 205 187 150 145 143 140 133 132 121 109 106 85 69 68 52 44 32 29 29 16 10 BspEI T'CCGG_A Cuts at: 6348 10119 6348 Size: 3771 6685 BspHI T'CATG_A Cuts at: 4114 4890 6237 8345 10075 4114 Size: 776 1347 2108 1730 4495 Fragments arranged by size: 4495 2108 1730 1347 776 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3871 4136 4548 4741 5054 5172 5363 7811 Size: 3027 265 412 193 313 118 191 2448 Cuts at: 7811 844 Size: 3489 Fragments arranged by size: 3489 3027 2448 412 313 265 193 191 118 BsrBI CCG'CTC Cuts at: 1394 1823 2115 4322 5994 8343 1394 Size: 429 292 2207 1672 2349 3507 Fragments arranged by size: 3507 2349 2207 1672 429 292 BsrDI GCAATG_nn' Cuts at: 65 1248 5220 5838 8951 9125 65 Size: 1183 3972 618 3113 174 1396 Fragments arranged by size: 3972 3113 1396 1183 618 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2165 3792 4991 5798 6242 7428 9100 Size: 110 355 1627 1199 807 444 1186 1672 Cuts at: 9100 9287 1700 Size: 187 2869 Fragments arranged by size: 2869 1672 1627 1199 1186 807 444 355 187 110 BsrGI T'GTAC_A Cuts at: 96 3339 96 Size: 3243 7213 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10456 BssSI C'ACGA_G Cuts at: 4832 8516 9528 4832 Size: 3684 1012 5760 Fragments arranged by size: 5760 3684 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4107 794 Size: 3313 7143 BstBI TT'CG_AA Cuts at: 2007 9260 2007 Size: 7253 3203 BstEII G'GTnAC_C Cuts at: 1184 3574 4981 7049 7064 1184 Size: 2390 1407 2068 15 4576 Fragments arranged by size: 4576 2390 2068 1407 15 BstXI CCAn_nnnn'nTGG Cuts at: 2308 4376 5003 5986 2308 Size: 2068 627 983 6778 Fragments arranged by size: 6778 2068 983 627 BstYI r'GATC_y Cuts at: 1972 2748 4194 5286 6609 7092 7623 7786 8153 Size: 776 1446 1092 1323 483 531 163 367 Cuts at: 8153 8538 8555 10007 10093 10105 10451 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1446 1323 1092 776 531 483 385 367 346 163 86 17 12 BstZ17I GTA'TAC Cuts at: 3609 6948 3609 Size: 3339 7117 Bsu36I CC'TnA_GG Cuts at: 2856 4449 2856 Size: 1593 8863 BtgI C'CryG_G Cuts at: 513 1065 3460 3588 4401 4702 5376 7045 7590 Size: 552 2395 128 813 301 674 1669 545 Cuts at: 7590 513 Size: 3379 Fragments arranged by size: 3379 2395 1669 813 674 552 545 301 128 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4780 5776 7504 487 Size: 683 56 3554 996 1728 3439 Fragments arranged by size: 3554 3439 1728 996 683 56 BtsI GCAGTG_nn' Cuts at: 3762 4042 6717 7972 8766 8786 3762 Size: 280 2675 1255 794 20 5432 Fragments arranged by size: 5432 2675 1255 794 280 20 ClaI AT'CG_AT Cuts at: 1554 3267 3718 6306 1554 Size: 1713 451 2588 5704 Fragments arranged by size: 5704 2588 1713 451 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3303 3338 6769 6804 529 Size: 35 2739 35 3431 35 4181 Fragments arranged by size: 4181 3431 2739 35 35 35 DraI TTT'AAA Cuts at: 7760 7760 Size: 10456 DraIII CAC_nnn'GTG Cuts at: 4747 7591 4747 Size: 2844 7612 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4460 5192 6065 9463 809 Size: 507 3144 732 873 3398 1802 Fragments arranged by size: 3398 3144 1802 873 732 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2865 3012 4264 Size: 54 1425 50 275 18 1035 147 1252 Cuts at: 4264 4398 4993 5230 5796 6701 7113 7200 7903 Size: 134 595 237 566 905 412 87 703 Cuts at: 7903 8792 10421 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1252 1035 905 889 703 595 566 412 275 237 147 134 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5796 6701 7903 1537 Size: 275 3984 905 1202 4090 Fragments arranged by size: 4090 3984 1202 905 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2325 2507 2618 2894 6446 8386 1353 Size: 972 182 111 276 3552 1940 3423 Fragments arranged by size: 3552 3423 1940 972 276 182 111 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 9043 9417 9563 387 Size: 1064 138 7454 374 146 1280 Fragments arranged by size: 7454 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3214 3633 5953 7657 3214 Size: 419 2320 1704 6013 Fragments arranged by size: 6013 2320 1704 419 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2120 2739 2901 3230 3303 3986 4324 Size: 393 488 619 162 329 73 683 338 Cuts at: 4324 5541 5579 5610 5930 5939 6101 6143 6203 Size: 1217 38 31 320 9 162 42 60 Cuts at: 6203 6338 6925 8519 9116 9903 10171 1239 Size: 135 587 1594 597 787 268 1524 Fragments arranged by size: 1594 1524 1217 787 683 619 597 587 488 393 338 329 320 268 162 162 135 73 60 42 38 31 9 EcoNI CCTnn'n_nnAGG Cuts at: 2465 2996 3866 6524 6940 7673 2465 Size: 531 870 2658 416 733 5248 Fragments arranged by size: 5248 2658 870 733 531 416 EcoO109I rG'GnC_Cy Cuts at: 2095 3328 3583 3640 4315 4453 4958 6118 6481 Size: 1233 255 57 675 138 505 1160 363 Cuts at: 6481 6482 6979 7665 7666 2095 Size: 1 497 686 1 4885 Fragments arranged by size: 4885 1233 1160 686 675 505 497 363 255 138 57 1 1 EcoRI G'AATT_C Cuts at: 7765 8143 7765 Size: 378 10078 EcoRV GAT'ATC Cuts at: 5965 5965 Size: 10456 FspI TGC'GCA Cuts at: 6427 8962 6427 Size: 2535 7921 HaeII r_GCGC'y Cuts at: 1678 3178 3257 3547 4074 6736 7191 9603 1678 Size: 1500 79 290 527 2662 455 2412 2531 Fragments arranged by size: 2662 2531 2412 1500 527 455 290 79 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2099 2131 2327 2359 2675 Size: 32 135 32 527 32 196 32 316 Cuts at: 2675 2707 2717 2749 3276 3308 4683 4715 5535 Size: 32 10 32 527 32 1375 32 820 Cuts at: 5535 5567 5890 5922 6811 6843 7433 7465 9097 Size: 32 323 32 889 32 590 32 1632 Cuts at: 9097 9129 9171 9203 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1632 1375 889 820 590 527 527 323 316 196 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 10 HincII GTy'rAC Cuts at: 669 1657 1938 2407 2554 4066 5140 5976 6583 Size: 988 281 469 147 1512 1074 836 607 Cuts at: 6583 7800 8046 669 Size: 1217 246 3079 Fragments arranged by size: 3079 1512 1217 1074 988 836 607 469 281 246 147 HindIII A'AGCT_T Cuts at: 748 5940 748 Size: 5192 5264 HpaI GTT'AAC Cuts at: 8046 8046 Size: 10456 KasI G'GCGC_C Cuts at: 3174 3253 6732 3174 Size: 79 3479 6898 Fragments arranged by size: 6898 3479 79 KpnI G_GTAC'C Cuts at: 7781 7781 Size: 10456 MfeI C'AATT_G Cuts at: 6385 8055 6385 Size: 1670 8786 MluI A'CGCG_T Cuts at: 10332 10332 Size: 10456 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4659 7476 9570 9754 1305 Size: 3354 2817 2094 184 2007 Fragments arranged by size: 3354 2817 2094 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2867 3014 5232 7115 7202 Size: 54 1425 343 1035 147 2218 1883 87 Cuts at: 7202 10 Size: 3264 Fragments arranged by size: 3264 2218 1883 1425 1035 343 147 87 54 MslI CAynn'nnrTG Cuts at: 518 1166 2306 2510 2627 4919 4979 6818 7265 Size: 648 1140 204 117 2292 60 1839 447 Cuts at: 7265 8414 8773 8932 518 Size: 1149 359 159 2042 Fragments arranged by size: 2292 2042 1839 1149 1140 648 447 359 204 159 117 60 MspA1I CmG'CkG Cuts at: 1606 2879 3943 3976 4080 4177 4489 4548 6289 Size: 1273 1064 33 104 97 312 59 1741 Cuts at: 6289 6700 7699 7872 8549 9697 9942 1606 Size: 411 999 173 677 1148 245 2120 Fragments arranged by size: 2120 1741 1273 1148 1064 999 677 411 312 245 173 104 97 59 33 NaeI GCC'GGC Cuts at: 2167 5800 2167 Size: 3633 6823 NarI GG'CG_CC Cuts at: 3175 3254 6733 3175 Size: 79 3479 6898 Fragments arranged by size: 6898 3479 79 NcoI C'CATG_G Cuts at: 513 1065 3460 4702 513 Size: 552 2395 1242 6267 Fragments arranged by size: 6267 2395 1242 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10456 NgoMIV G'CCGG_C Cuts at: 2165 5798 2165 Size: 3633 6823 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10456 NotI GC'GGCC_GC Cuts at: 7903 7903 Size: 10456 NsiI A_TGCA'T Cuts at: 6825 6825 Size: 10456 NspI r_CATG'y Cuts at: 1132 1229 2173 3676 7216 7606 7814 9359 1132 Size: 97 944 1503 3540 390 208 1545 2229 Fragments arranged by size: 3540 2229 1545 1503 944 390 208 97 PasI CC'CwG_GG Cuts at: 1348 3304 4781 1348 Size: 1956 1477 7023 Fragments arranged by size: 7023 1956 1477 PciI A'CATG_T Cuts at: 3672 7212 9355 3672 Size: 3540 2143 4773 Fragments arranged by size: 4773 3540 2143 PflMI CCAn_nnn'nTGG Cuts at: 1108 3304 4599 1108 Size: 2196 1295 6965 Fragments arranged by size: 6965 2196 1295 PfoI T'CCnGG_A Cuts at: 3516 3887 4137 6908 3516 Size: 371 250 2771 7064 Fragments arranged by size: 7064 2771 371 250 PmeI GTTT'AAAC Cuts at: 7760 7760 Size: 10456 PmlI CAC'GTG Cuts at: 5713 6109 5713 Size: 396 10060 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7148 7180 8525 8557 10038 10070 7148 Size: 32 1345 32 1481 32 7534 Fragments arranged by size: 7534 1481 1345 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3583 3640 4315 4453 4958 3583 Size: 57 675 138 505 9081 Fragments arranged by size: 9081 675 505 138 57 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10456 PsiI TTA'TAA Cuts at: 7710 8026 7710 Size: 316 10140 PspOMI G'GGCC_C Cuts at: 2094 3917 6481 6978 7665 2094 Size: 1823 2564 497 687 4885 Fragments arranged by size: 4885 2564 1823 687 497 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4349 4381 4349 Size: 32 10424 PstI C_TGCA'G Cuts at: 830 2378 3000 4037 5169 6192 6252 7134 7808 Size: 1548 622 1037 1132 1023 60 882 674 Cuts at: 7808 830 Size: 3478 Fragments arranged by size: 3478 1548 1132 1037 1023 882 674 622 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10456 PvuII CAG'CTG Cuts at: 3976 4177 6289 3976 Size: 201 2112 8143 Fragments arranged by size: 8143 2112 201 SacI G_AGCT'C Cuts at: 3216 3635 5955 7659 3216 Size: 419 2320 1704 6013 Fragments arranged by size: 6013 2320 1704 419 SalI G'TCGA_C Cuts at: 1936 5138 7798 1936 Size: 3202 2660 4594 Fragments arranged by size: 4594 3202 2660 SanDI GG'GwC_CC Cuts at: 3583 3640 4315 3583 Size: 57 675 9724 Fragments arranged by size: 9724 675 57 SapI GCTCTTCn'nnn_ Cuts at: 2618 2618 Size: 10456 SbfI CC_TGCA'GG Cuts at: 3000 6252 7808 3000 Size: 3252 1556 5648 Fragments arranged by size: 5648 3252 1556 ScaI AGT'ACT Cuts at: 8704 10416 8704 Size: 1712 8744 SfcI C'TryA_G Cuts at: 826 1045 2374 2996 3179 3394 3971 4033 5165 Size: 219 1329 622 183 215 577 62 1132 Cuts at: 5165 6188 6248 7130 7804 8939 9620 9811 826 Size: 1023 60 882 674 1135 681 191 1471 Fragments arranged by size: 1471 1329 1135 1132 1023 882 681 674 622 577 219 215 191 183 62 60 SfiI GGCCn_nnn'nGGCC Cuts at: 6252 6252 Size: 10456 SfoI GGC'GCC Cuts at: 3176 3255 6734 3176 Size: 79 3479 6898 Fragments arranged by size: 6898 3479 79 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10456 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10456 SmaI CCC'GGG Cuts at: 1872 2093 2972 6048 6752 6977 7783 1872 Size: 221 879 3076 704 225 806 4545 Fragments arranged by size: 4545 3076 879 806 704 225 221 SmlI C'TyrA_G Cuts at: 820 1017 1943 3494 4232 4511 5859 5879 6281 Size: 197 926 1551 738 279 1348 20 402 Cuts at: 6281 8560 9461 9723 820 Size: 2279 901 262 1553 Fragments arranged by size: 2279 1553 1551 1348 926 901 738 402 279 262 197 20 SnaBI TAC'GTA Cuts at: 493 7753 8247 493 Size: 7260 494 2702 Fragments arranged by size: 7260 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 10456 SphI G_CATG'C Cuts at: 1132 2173 7814 1132 Size: 1041 5641 3774 Fragments arranged by size: 5641 3774 1041 SspI AAT'ATT Cuts at: 5 52 4156 8380 5 Size: 47 4104 4224 2081 Fragments arranged by size: 4224 4104 2081 47 StuI AGG'CCT Cuts at: 1517 3041 3704 1517 Size: 1524 663 8269 Fragments arranged by size: 8269 1524 663 StyI C'CwwG_G Cuts at: 513 1065 2236 3249 3460 3921 3984 4702 5002 Size: 552 1171 1013 211 461 63 718 300 Cuts at: 5002 5110 5282 5925 6736 7119 7470 7895 513 Size: 108 172 643 811 383 351 425 3074 Fragments arranged by size: 3074 1171 1013 811 718 643 552 461 425 383 351 300 211 172 108 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2131 5157 7300 7486 8483 8500 8653 Size: 1255 551 3026 2143 186 997 17 153 Cuts at: 8653 8838 9283 325 Size: 185 445 1498 Fragments arranged by size: 3026 2143 1498 1255 997 551 445 186 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3067 3339 4911 Size: 274 80 33 51 1443 1090 272 1572 Cuts at: 4911 5175 6555 7209 8702 10414 96 Size: 264 1380 654 1493 1712 138 Fragments arranged by size: 1712 1572 1493 1443 1380 1090 654 274 272 264 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2528 2560 3227 3259 3406 3438 6713 6745 10416 Size: 32 667 32 147 32 3275 32 3671 Cuts at: 10416 10448 2528 Size: 32 2536 Fragments arranged by size: 3671 3275 2536 667 147 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2659 4663 4897 6358 1316 Size: 1343 2004 234 1461 5414 Fragments arranged by size: 5414 2004 1461 1343 234 XbaI T'CTAG_A Cuts at: 7792 7792 Size: 10456 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3586 3586 Size: 10456 XhoI C'TCGA_G Cuts at: 1943 5859 1943 Size: 3916 6540 XmaI C'CCGG_G Cuts at: 1870 2091 2970 6046 6750 6975 7781 1870 Size: 221 879 3076 704 225 806 4545 Fragments arranged by size: 4545 3076 879 806 704 225 221 XmnI GAAnn'nnTTC Cuts at: 8585 8585 Size: 10456 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3238 6915 8264 276 Size: 53 83 186 936 1704 3677 1349 2468 Fragments arranged by size: 3677 2468 1704 1349 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BsiWI FalI FseI FspAI NruI PacI PspXI RsrII SacII SexAI SrfI SwaI