Restriction map for
pFN21AB8804
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII BamHI BanI BanII BbeI
BbrPI BbvCI BciVI BcnI BfrI
BglI BmyI BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1286I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MfeI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TspRI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AciI 567 CCGC CCGC(-3/-1)
AlwI 464 GGATC GGATC(4/5)
AlwNI 382 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AvrII 573 CCTAGG C^CTAGG
BalI 950 TGGCCA TGG^CCA
BbsI 974 GAAGAC GAAGAC(2/6)
BcgI 839 GCATGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 812 TGATCA T^GATCA
BglII 611 AGATCT A^GATCT
BlnI 573 CCTAGG C^CTAGG
BlpI 501 GCTGAGC GC^T[ACGT]AGC
BpmI 969 CTGGAG CTGGAG(16/14)
Bpu1102I 501 GCTGAGC GC^T[ACGT]AGC
BpuAI 974 GAAGAC GAAGAC(2/6)
BsaBI 87 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 994 GAGGAG GAGGAG(10/8)
BsiYI 240 CCTGAATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 240 CCTGAATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 430 GTCCC GGGAC(10/14)
BspHI 680 TCATGA T^CATGA
BssKI 968 CCTGG ^CC[ACGT]GG
BstAPI 190 GCAATTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 968 CCTGG CC^[AT]GG
BstXI 179 CCATGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 611 AGATCT [GA]^GATC[CT]
Cac8I 169 GCAGGC GC[ACGT]^[ACGT]GC
CelII 501 GCTGAGC GC^T[ACGT]AGC
DsaI 179 CCATGG C^C[GA][CT]GG
EaeI 950 TGGCCA [CT]^GGCC[GA]
EcoRII 968 CCTGG ^CC[AT]GG
FauI 566 CCCGC CCCGC(4/6)
FbaI 812 TGATCA T^GATCA
HaeIII 951 GGCC GG^CC
HincII 21 GTCAAC GT[CT]^[GA]AC
HindII 21 GTCAAC GT[CT]^[GA]AC
HphI 815 TCACC GGTGA(8/7)
MaeII 938 ACGT A^CGT
MamI 87 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 611 AGATCT [GA]^GATC[CT]
MluNI 950 TGGCCA TGG^CCA
MscI 950 TGGCCA TGG^CCA
MvaI 968 CCTGG CC^[AT]GG
NcoI 179 CCATGG C^CATGG
PshAI 67 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 680 TCATGA T^CATGA
ScaI 1036 AGTACT AGT^ACT
ScrFI 968 CCTGG CC^[ACGT]GG
TaqI 708 TCGA T^CGA
TthHB8I 708 TCGA T^CGA
XhoII 611 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 112 GAATTT [GA]^AATT[CT]
243 GAATTT
ApoI 112 GAATTT [GA]^AATT[CT]
243 GAATTT
AvaII 965 GGTCC G^G[AT]CC
1018 GGTCC
BsaJI 179 CCATGG C^C[ACGT][ACGT]GG
573 CCTAGG
BsmAI 981 GAGAC GTCTC(1/5)
370 GTCTC
BsmI 377 GAATGC GAATGC(1/-1)
551 GCATTC
Bsp1407I 233 TGTACA T^GTACA
353 TGTACA
BsrGI 233 TGTACA T^GTACA
353 TGTACA
BsrI 18 CCAGT ACTGG(1/-1)
1034 CCAGT
Cfr13I 965 GGTCC G^G[ACGT]CC
1018 GGTCC
DdeI 502 CTGAG C^T[ACGT]AG
1013 CTGAG
DraII 964 GGGTCCT [GA]G^G[ACGT]CC[CT]
1017 GGGTCCT
EcoO109I 964 GGGTCCT [GA]G^G[ACGT]CC[CT]
1017 GGGTCCT
EcoT14I 179 CCATGG C^C[AT][AT]GG
573 CCTAGG
HinfI 597 GATTC G^A[ACGT]TC
1009 GATTC
MseI 296 TTAA T^TAA
698 TTAA
MslI 180 CATGGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
681 CATGAAGATG
NlaIV 964 GGGTCC GG[ACGT]^[ACGT]CC
1017 GGGTCC
PpuMI 964 GGGTCCT [GA]G^G[AT]CC[CT]
1017 GGGTCCT
Sau96I 965 GGTCC G^G[ACGT]CC
1018 GGTCC
SspBI 233 TGTACA T^GTACA
353 TGTACA
StyI 179 CCATGG C^C[AT][AT]GG
573 CCTAGG
TfiI 597 GATTC G^A[AT]TC
1009 GATTC
Tru9I 296 TTAA T^TAA
698 TTAA
Tsp45I 751 GTCAC ^GT[GC]AC
859 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AfaI AluI BbvI BfaI BstF5I
DpnII Fnu4HI FokI ItaI MaeI
MaeIII MboI MboII MnlI MspA1I
MwoI NdeII NlaIII PvuII RsaI
Sau3AI SfaNI SfcI TseI Tsp509I