Restriction map for
pFN21ASDA0441
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbvCI BfrI BglII
BlnI BsaBI BsaHI BsaI BseAI
BsiWI BsmBI BsmFI BspDI BspEI
BspHI BspLU11I BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Cfr10I ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Alw44I 1903 GTGCAC G^TGCAC
AlwNI 2005 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 874 CCTGAGG CC^T[ACGT]AGG
ApaLI 1903 GTGCAC G^TGCAC
AvaI 1638 CTCGGG C^[CT]CG[GA]G
BanI 430 GGTGCC G^G[CT][GA]CC
BbsI 488 GAAGAC GAAGAC(2/6)
BcgI 4 GCAGAAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 263 GTATCC GTATCC(6/5)
BfaI 1706 CTAG C^TAG
BlpI 2002 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2002 GCTCAGC GC^T[ACGT]AGC
BpuAI 488 GAAGAC GAAGAC(2/6)
BsaWI 1215 ACCGGA [AT]^CCGG[AT]
BsiEI 1042 CGGCCG CG[GA][CT]^CG
BsoBI 1638 CTCGGG C^[CT]CG[GA]G
BsrBI 1039 GAGCGG CCGCTC(-3/-3)
BsrDI 509 CATTGC GCAATG(2/0)
Bsu36I 874 CCTGAGG CC^T[ACGT]AGG
CelII 2002 GCTCAGC GC^T[ACGT]AGC
CfoI 861 GCGC GCG^C
DsaI 825 CCATGG C^C[GA][CT]GG
EagI 1042 CGGCCG C^GGCCG
EclXI 1042 CGGCCG C^GGCCG
Eco52I 1042 CGGCCG C^GGCCG
Eco81I 874 CCTGAGG CC^T[ACGT]AGG
EcoNI 360 CCTGGTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 881 CCCGC CCCGC(4/6)
HaeII 860 AGCGCC [GA]GCGC^[CT]
HgaI 49 GACGC GACGC(5/10)
HhaI 861 GCGC GCG^C
HinP1I 861 GCGC G^CGC
MaeI 1706 CTAG C^TAG
NcoI 825 CCATGG C^CATGG
NspI 1548 GCATGC [GA]CATG^[CT]
PshAI 1121 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 2014 CAGCTG CAG^CTG
SacI 1969 GAGCTC GAGCT^C
ScaI 1987 AGTACT AGT^ACT
SexAI 359 ACCTGGT A^CC[AT]GGT
SphI 1548 GCATGC GCATG^C
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# TWO CUT ENZYMES ( 24 enzymes) #
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ApaI 146 GGGCCC GGGCC^C
1882 GGGCCC
BbrPI 1906 CACGTG CAC^GTG
2053 CACGTG
BclI 1140 TGATCA T^GATCA
1910 TGATCA
BcnI 144 CCGGG CC^[GC]GG
1699 CCCGG
BglI 21 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
863 GCCCTGGAGGC
BsaAI 1906 CACGTG [CT]AC^GT[GA]
2053 CACGTG
BsmI 1219 GAATGC GAATGC(1/-1)
1442 GCATTC
BspMI 1051 ACCTGC ACCTGC(4/8)
2009 ACCTGC
DraIII 1056 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2050 CACCACGTG
EcoT14I 825 CCATGG C^C[AT][AT]GG
1922 CCAAGG
FbaI 1140 TGATCA T^GATCA
1910 TGATCA
HphI 1288 GGTGA GGTGA(8/7)
2049 TCACC
MaeII 1907 ACGT A^CGT
2054 ACGT
MspA1I 882 CCGCTG C[AC]G^C[GT]G
2014 CAGCTG
NciI 144 CCGGG CC^[GC]GG
1699 CCCGG
PleI 1417 GACTC GAGTC(4/5)
750 GAGTC
PmaCI 1906 CACGTG CAC^GTG
2053 CACGTG
PmlI 1906 CACGTG CAC^GTG
2053 CACGTG
SfaNI 1026 GATGC GCATC(5/9)
1558 GATGC
SspI 1154 AATATT AAT^ATT
1603 AATATT
StyI 825 CCATGG C^C[AT][AT]GG
1922 CCAAGG
TaqI 753 TCGA T^CGA
1368 TCGA
TthHB8I 753 TCGA T^CGA
1368 TCGA
XcmI 224 CCATTTATATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1922 CCAAGGAAACACTGG
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
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AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BalI
BanII BbvI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI Ksp632I MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfcI SspBI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII XmnI