(Circular) (Six-base) MAPSORT of: pFN21ASDA0436.seq Check: 4649 from: 1 to: 6643 pFN21ASDA0436 6643 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4453 278 Size: 53 83 186 936 2917 2468 Fragments arranged by size: 2917 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3986 1937 Size: 2049 4594 Acc65I G'GTAC_C Cuts at: 3697 3964 3697 Size: 267 6376 AclI AA'CG_TT Cuts at: 1563 3619 4770 5143 1563 Size: 2056 1151 373 3063 Fragments arranged by size: 3063 2056 1151 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2304 2313 2601 2864 4706 6090 6358 1632 Size: 672 9 288 263 1842 1384 268 1917 Fragments arranged by size: 1917 1842 1384 672 288 268 263 9 AflII C'TTAA_G Cuts at: 820 1017 2207 3917 820 Size: 197 1190 1710 3546 Fragments arranged by size: 3546 1710 1190 197 AflIII A'CryG_T Cuts at: 1203 2814 3184 5542 6519 1203 Size: 1611 370 2358 977 1327 Fragments arranged by size: 2358 1611 1327 977 370 AgeI A'CCGG_T Cuts at: 5474 5474 Size: 6643 AhdI GACnn_n'nnGTC Cuts at: 5372 5372 Size: 6643 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6643 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6643 AlwNI CAG_nnn'CTG Cuts at: 2741 4386 5958 2741 Size: 1645 1572 3426 Fragments arranged by size: 3426 1645 1572 ApaLI G'TGCA_C Cuts at: 4700 5856 4700 Size: 1156 5487 ApoI r'AATT_y Cuts at: 1493 2717 2947 3220 3866 3932 3952 4178 4330 Size: 1224 230 273 646 66 20 226 152 Cuts at: 4330 6439 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1224 646 273 230 226 152 66 20 AseI AT'TA_AT Cuts at: 160 1032 5197 160 Size: 872 4165 1606 Fragments arranged by size: 4165 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6643 AvaI C'yCGr_G Cuts at: 1870 1943 3968 1870 Size: 73 2025 4545 Fragments arranged by size: 4545 2025 73 BamHI G'GATC_C Cuts at: 3973 4340 3973 Size: 367 6276 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3502 3697 3964 5419 618 Size: 325 217 627 1715 195 267 1455 1842 Fragments arranged by size: 1842 1715 1455 627 325 267 217 195 BanII G_rGCy'C Cuts at: 1391 2609 3962 1391 Size: 1218 1353 4072 Fragments arranged by size: 4072 1353 1218 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3063 928 Size: 971 1164 4508 Fragments arranged by size: 4508 1164 971 BbvCI CC'TCA_GC Cuts at: 3648 3648 Size: 6643 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4834 4868 1903 Size: 34 2897 34 3678 Fragments arranged by size: 3678 2897 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2935 4535 5745 2935 Size: 1600 1210 3833 Fragments arranged by size: 3833 1600 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6643 BfrBI ATG'CAT Cuts at: 2030 2030 Size: 6643 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3235 5254 136 Size: 107 122 71 631 605 1563 2019 1525 Fragments arranged by size: 2019 1563 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6638 6638 Size: 6643 BlpI GC'TnA_GC Cuts at: 2321 4060 2321 Size: 1739 4904 Bme1580I G_kGCm'C Cuts at: 1790 4704 5860 1790 Size: 2914 1156 2573 Fragments arranged by size: 2914 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6643 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3057 4374 5332 Size: 416 53 83 399 24 1640 1317 958 Cuts at: 5332 442 Size: 1753 Fragments arranged by size: 1753 1640 1317 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6643 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2232 3290 3456 3834 5303 1239 Size: 993 1058 166 378 1469 2579 Fragments arranged by size: 2579 1469 1058 993 378 166 Bpu10I CC'TnA_GC Cuts at: 2603 3648 3759 2603 Size: 1045 111 5487 Fragments arranged by size: 5487 1045 111 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3719 4768 5633 5931 3719 Size: 1049 865 298 4431 Fragments arranged by size: 4431 1049 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2907 5306 882 Size: 617 1408 2399 2219 Fragments arranged by size: 2399 2219 1408 617 BsaAI yAC'GTr Cuts at: 493 1204 3940 4434 493 Size: 711 2736 494 2702 Fragments arranged by size: 2736 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6637 6637 Size: 6643 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4450 4832 5467 275 Size: 53 83 186 936 2917 382 635 1451 Fragments arranged by size: 2917 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5076 5474 5748 5895 6306 5076 Size: 398 274 147 411 5413 Fragments arranged by size: 5413 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6418 Fragments arranged by size: 6418 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6643 BseYI C'CCAG_C Cuts at: 5846 6342 5846 Size: 496 6147 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6070 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4093 4854 5882 1540 Size: 275 186 2092 761 1028 2301 Fragments arranged by size: 2301 2092 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4704 4789 5860 4704 Size: 85 1071 5487 Fragments arranged by size: 5487 1071 85 BsmI GAATG_Cn' Cuts at: 4154 4247 4154 Size: 93 6550 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2609 3962 4704 4789 5860 1391 Size: 399 819 1353 742 85 1071 2174 Fragments arranged by size: 2174 1353 1071 819 742 399 85 BspEI T'CCGG_A Cuts at: 6306 6306 Size: 6643 BspHI T'CATG_A Cuts at: 2437 4532 6262 2437 Size: 2095 1730 2818 Fragments arranged by size: 2818 2095 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3998 844 Size: 3154 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3488 4530 1394 Size: 429 1665 1042 3507 Fragments arranged by size: 3507 1665 1042 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5138 5312 65 Size: 1183 3890 174 1396 Fragments arranged by size: 3890 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5287 5474 1700 Size: 110 3477 187 2869 Fragments arranged by size: 3477 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6643 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6643 BssSI C'ACGA_G Cuts at: 4703 5715 4703 Size: 1012 5631 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6643 BstBI TT'CG_AA Cuts at: 2007 5447 2007 Size: 3440 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6643 BstYI r'GATC_y Cuts at: 1972 3913 3973 4340 4725 4742 6194 6280 6292 Size: 1941 60 367 385 17 1452 86 12 Cuts at: 6292 6638 1972 Size: 346 1977 Fragments arranged by size: 1977 1941 1452 385 367 346 86 60 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 5134 Fragments arranged by size: 5134 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3730 487 Size: 683 56 2504 3400 Fragments arranged by size: 3400 2504 683 56 BtsI GCAGTG_nn' Cuts at: 4159 4953 4973 4159 Size: 794 20 5829 Fragments arranged by size: 5829 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6643 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6608 DraI TTT'AAA Cuts at: 3947 3947 Size: 6643 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5650 809 Size: 507 4334 1802 Fragments arranged by size: 4334 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3322 3385 4090 Size: 54 1425 50 275 18 1492 63 705 Cuts at: 4090 4979 6608 8 Size: 889 1629 43 Fragments arranged by size: 1629 1492 1425 889 705 275 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4090 1537 Size: 275 2278 4090 Fragments arranged by size: 4090 2278 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2780 4573 1353 Size: 1427 1793 3423 Fragments arranged by size: 3423 1793 1427 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5230 5604 5750 387 Size: 1064 138 3641 374 146 1280 Fragments arranged by size: 3641 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2232 2304 2313 2601 2864 3290 3456 Size: 393 600 72 9 288 263 426 166 Cuts at: 3456 3834 4706 5303 6090 6358 1239 Size: 378 872 597 787 268 1524 Fragments arranged by size: 1524 872 787 600 597 426 393 378 288 268 263 166 72 9 EcoNI CCTnn'n_nnAGG Cuts at: 2419 3705 3875 2419 Size: 1286 170 5187 Fragments arranged by size: 5187 1286 170 EcoO109I rG'GnC_Cy Cuts at: 2614 2650 3241 2614 Size: 36 591 6016 Fragments arranged by size: 6016 591 36 EcoRI G'AATT_C Cuts at: 3952 4330 3952 Size: 378 6265 FspI TGC'GCA Cuts at: 5149 5149 Size: 6643 HaeII r_GCGC'y Cuts at: 1678 5790 1678 Size: 4112 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5284 5316 5358 5390 1373 Size: 32 135 32 3712 32 42 32 2626 Fragments arranged by size: 3712 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3987 4233 669 Size: 988 281 2049 246 3079 Fragments arranged by size: 3079 2049 988 281 246 HindIII A'AGCT_T Cuts at: 748 2343 748 Size: 1595 5048 HpaI GTT'AAC Cuts at: 4233 4233 Size: 6643 KpnI G_GTAC'C Cuts at: 3701 3968 3701 Size: 267 6376 MfeI C'AATT_G Cuts at: 4242 4242 Size: 6643 MluI A'CGCG_T Cuts at: 6519 6519 Size: 6643 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2683 5757 5941 1305 Size: 1378 3074 184 2007 Fragments arranged by size: 3074 2007 1378 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3387 10 Size: 54 1425 343 1555 3266 Fragments arranged by size: 3266 1555 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2090 2673 3189 3318 3530 4601 4960 Size: 648 924 583 516 129 212 1071 359 Cuts at: 4960 5119 518 Size: 159 2042 Fragments arranged by size: 2042 1071 924 648 583 516 359 212 159 129 MspA1I CmG'CkG Cuts at: 1606 2738 4059 4736 5884 6129 1606 Size: 1132 1321 677 1148 245 2120 Fragments arranged by size: 2120 1321 1148 1132 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5134 Fragments arranged by size: 5134 957 552 NdeI CA'TA_TG Cuts at: 387 3679 387 Size: 3292 3351 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6643 NotI GC'GGCC_GC Cuts at: 4090 4090 Size: 6643 NsiI A_TGCA'T Cuts at: 2032 2032 Size: 6643 NspI r_CATG'y Cuts at: 1132 1229 2818 3188 3758 4001 5546 1132 Size: 97 1589 370 570 243 1545 2229 Fragments arranged by size: 2229 1589 1545 570 370 243 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6643 PciI A'CATG_T Cuts at: 2814 3184 5542 2814 Size: 370 2358 3915 Fragments arranged by size: 3915 2358 370 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6643 PmeI GTTT'AAAC Cuts at: 3947 3947 Size: 6643 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4712 4744 6225 6257 4712 Size: 32 1481 32 5098 Fragments arranged by size: 5098 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2614 2650 3241 2614 Size: 36 591 6016 Fragments arranged by size: 6016 591 36 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6643 PsiI TTA'TAA Cuts at: 2315 4213 2315 Size: 1898 4745 PstI C_TGCA'G Cuts at: 830 3995 830 Size: 3165 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6643 SalI G'TCGA_C Cuts at: 1936 3985 1936 Size: 2049 4594 SanDI GG'GwC_CC Cuts at: 2614 2614 Size: 6643 SbfI CC_TGCA'GG Cuts at: 3995 3995 Size: 6643 ScaI AGT'ACT Cuts at: 2625 4891 6603 2625 Size: 2266 1712 2665 Fragments arranged by size: 2665 2266 1712 SfcI C'TryA_G Cuts at: 826 1045 2712 3991 5126 5807 5998 826 Size: 219 1667 1279 1135 681 191 1471 Fragments arranged by size: 1667 1471 1279 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6643 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6643 SmaI CCC'GGG Cuts at: 1872 3970 1872 Size: 2098 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2207 3734 3917 4747 5648 5910 Size: 197 926 264 1527 183 830 901 262 Cuts at: 5910 820 Size: 1553 Fragments arranged by size: 1553 1527 926 901 830 264 262 197 183 SnaBI TAC'GTA Cuts at: 493 3940 4434 493 Size: 3447 494 2702 Fragments arranged by size: 3447 2702 494 SpeI A'CTAG_T Cuts at: 152 3074 152 Size: 2922 3721 SphI G_CATG'C Cuts at: 1132 3758 4001 1132 Size: 2626 243 3774 Fragments arranged by size: 3774 2626 243 SspI AAT'ATT Cuts at: 5 52 2560 3005 3640 3793 4567 5 Size: 47 2508 445 635 153 774 2081 Fragments arranged by size: 2508 2081 774 635 445 153 47 StuI AGG'CCT Cuts at: 1517 3229 3303 1517 Size: 1712 74 4857 Fragments arranged by size: 4857 1712 74 StyI C'CwwG_G Cuts at: 513 1065 2022 2609 2918 3388 4082 513 Size: 552 957 587 309 470 694 3074 Fragments arranged by size: 3074 957 694 587 552 470 309 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2889 4670 4687 4840 5025 5470 325 Size: 1255 1309 1781 17 153 185 445 1498 Fragments arranged by size: 1781 1498 1309 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2623 4889 6601 Size: 274 80 33 51 1443 646 2266 1712 Cuts at: 6601 96 Size: 138 Fragments arranged by size: 2266 1712 1443 646 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3105 3137 3529 3561 6603 6635 3105 Size: 32 392 32 3042 32 3113 Fragments arranged by size: 3113 3042 392 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6643 XbaI T'CTAG_A Cuts at: 3091 3799 3979 3091 Size: 708 180 5755 Fragments arranged by size: 5755 708 180 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3284 3284 Size: 6643 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6643 XmaI C'CCGG_G Cuts at: 1870 3968 1870 Size: 2098 4545 XmnI GAAnn'nnTTC Cuts at: 4772 4772 Size: 6643 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4451 276 Size: 53 83 186 936 2917 2468 Fragments arranged by size: 2917 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI