(Circular) (Six-base) MAPSORT of: pFN21ASDA0427.seq Check: 4298 from: 1 to: 6523 pFN21ASDA0427 6523 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3545 3545 Size: 6523 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4333 278 Size: 53 83 186 936 2797 2468 Fragments arranged by size: 2797 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3866 1937 Size: 1929 4594 Acc65I G'GTAC_C Cuts at: 3844 3844 Size: 6523 AclI AA'CG_TT Cuts at: 1563 4650 5023 1563 Size: 3087 373 3063 Fragments arranged by size: 3087 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2329 4586 5970 6238 1632 Size: 697 2257 1384 268 1917 Fragments arranged by size: 2257 1917 1384 697 268 AfeI AGC'GCT Cuts at: 2099 3440 2099 Size: 1341 5182 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6326 AflIII A'CryG_T Cuts at: 1203 5422 6399 1203 Size: 4219 977 1327 Fragments arranged by size: 4219 1327 977 AgeI A'CCGG_T Cuts at: 5354 5354 Size: 6523 AhdI GACnn_n'nnGTC Cuts at: 5252 5252 Size: 6523 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6523 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6523 AlwNI CAG_nnn'CTG Cuts at: 2140 2238 2431 3808 4266 5838 2140 Size: 98 193 1377 458 1572 2825 Fragments arranged by size: 2825 1572 1377 458 193 98 ApaLI G'TGCA_C Cuts at: 4580 5736 4580 Size: 1156 5367 ApoI r'AATT_y Cuts at: 1493 3832 4058 4210 6319 1493 Size: 2339 226 152 2109 1697 Fragments arranged by size: 2339 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5077 160 Size: 872 4045 1606 Fragments arranged by size: 4045 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6523 AvaI C'yCGr_G Cuts at: 1870 1943 2250 2903 3014 3848 1870 Size: 73 307 653 111 834 4545 Fragments arranged by size: 4545 834 653 307 111 73 BamHI G'GATC_C Cuts at: 3853 4220 3853 Size: 367 6156 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2351 2772 3479 3844 5299 Size: 325 217 627 564 421 707 365 1455 Cuts at: 5299 618 Size: 1842 Fragments arranged by size: 1842 1455 707 627 564 421 365 325 217 BanII G_rGCy'C Cuts at: 1391 2263 2714 3507 3552 3660 3741 3842 1391 Size: 872 451 793 45 108 81 101 4072 Fragments arranged by size: 4072 872 793 451 108 101 81 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3048 3466 928 Size: 971 1149 418 3985 Fragments arranged by size: 3985 1149 971 418 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4714 4748 1903 Size: 34 2777 34 3678 Fragments arranged by size: 3678 2777 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4415 5625 4415 Size: 1210 5313 BclI T'GATC_A Cuts at: 1548 1548 Size: 6523 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2939 5134 136 Size: 107 122 71 631 605 1267 2195 1525 Fragments arranged by size: 2195 1525 1267 631 605 122 107 71 BglII A'GATC_T Cuts at: 6518 6518 Size: 6523 BlpI GC'TnA_GC Cuts at: 2713 3940 2713 Size: 1227 5296 Bme1580I G_kGCm'C Cuts at: 1790 2312 2895 3527 3692 4584 5740 1790 Size: 522 583 632 165 892 1156 2573 Fragments arranged by size: 2573 1156 892 632 583 522 165 BmgBI CAC'GTC Cuts at: 1318 3187 3433 1318 Size: 1869 246 4408 Fragments arranged by size: 4408 1869 246 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2179 2194 2386 Size: 416 53 83 399 24 762 15 192 Cuts at: 2386 4254 5212 442 Size: 1868 958 1753 Fragments arranged by size: 1868 1753 958 762 416 399 192 83 53 24 15 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6523 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2757 2757 Size: 6523 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 2141 2888 3035 3647 5183 1239 Size: 875 27 747 147 612 1536 2579 Fragments arranged by size: 2579 1536 875 747 612 147 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4648 5513 5811 4648 Size: 865 298 5360 Fragments arranged by size: 5360 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2611 2834 5186 882 Size: 617 1112 223 2352 2219 Fragments arranged by size: 2352 2219 1112 617 223 BsaAI yAC'GTr Cuts at: 493 1204 2116 3820 4314 493 Size: 711 912 1704 494 2702 Fragments arranged by size: 2702 1704 912 711 494 BsaBI GATnn'nnATC Cuts at: 3152 6517 3152 Size: 3365 3158 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3767 4330 4712 5347 Size: 53 83 186 936 2234 563 382 635 Cuts at: 5347 275 Size: 1451 Fragments arranged by size: 2234 1451 936 635 563 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3366 4956 5354 5628 5775 6186 3366 Size: 1590 398 274 147 411 3703 Fragments arranged by size: 3703 1590 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3103 3133 1178 Size: 30 165 30 1700 30 4568 Fragments arranged by size: 4568 1700 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2041 2102 2605 3230 3257 3368 3428 Size: 837 18 61 503 625 27 111 60 Cuts at: 3428 1186 Size: 4281 Fragments arranged by size: 4281 837 625 503 111 61 60 27 18 BseYI C'CCAG_C Cuts at: 2305 3123 3250 3445 5726 6222 2305 Size: 818 127 195 2281 496 2606 Fragments arranged by size: 2606 2281 818 496 195 127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 1163 Size: 573 417 5533 Fragments arranged by size: 5533 573 417 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2446 3295 3716 3973 4734 5762 Size: 275 186 445 849 421 257 761 1028 Cuts at: 5762 1540 Size: 2301 Fragments arranged by size: 2301 1028 849 761 445 421 275 257 186 BsiHKAI G_wGCw'C Cuts at: 3519 3552 3660 3741 4584 4669 5740 3519 Size: 33 108 81 843 85 1071 4302 Fragments arranged by size: 4302 1071 843 108 85 81 33 BsmI GAATG_Cn' Cuts at: 4034 4127 4034 Size: 93 6430 BsmBI CGTCTCn'nnnn_ Cuts at: 3104 3104 Size: 6523 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2263 2312 2714 2895 3507 3519 3527 Size: 399 473 49 402 181 612 12 8 Cuts at: 3527 3552 3660 3692 3741 3842 4584 4669 5740 Size: 25 108 32 49 101 742 85 1071 Cuts at: 5740 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 612 473 402 399 181 108 101 85 49 49 32 25 12 8 BspEI T'CCGG_A Cuts at: 3366 6186 3366 Size: 2820 3703 BspHI T'CATG_A Cuts at: 4412 6142 4412 Size: 1730 4793 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2373 3545 3878 844 Size: 1529 1172 333 3489 Fragments arranged by size: 3489 1529 1172 333 BsrBI CCG'CTC Cuts at: 1394 1823 2073 2950 3031 4410 1394 Size: 429 250 877 81 1379 3507 Fragments arranged by size: 3507 1379 877 429 250 81 BsrDI GCAATG_nn' Cuts at: 65 1248 2836 5018 5192 65 Size: 1183 1588 2182 174 1396 Fragments arranged by size: 2182 1588 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2763 3618 5167 5354 1700 Size: 110 953 855 1549 187 2869 Fragments arranged by size: 2869 1549 953 855 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6523 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6523 BssSI C'ACGA_G Cuts at: 3518 4583 5595 3518 Size: 1065 1012 4446 Fragments arranged by size: 4446 1065 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2140 2431 794 Size: 1346 291 4886 Fragments arranged by size: 4886 1346 291 BstBI TT'CG_AA Cuts at: 2007 5327 2007 Size: 3320 3203 BstEII G'GTnAC_C Cuts at: 1184 2651 1184 Size: 1467 5056 BstXI CCAn_nnnn'nTGG Cuts at: 2648 3211 2648 Size: 563 5960 BstYI r'GATC_y Cuts at: 1972 3153 3853 4220 4605 4622 6074 6160 6172 Size: 1181 700 367 385 17 1452 86 12 Cuts at: 6172 6518 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1181 700 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2728 3542 2728 Size: 814 5709 BtgI C'CryG_G Cuts at: 513 1065 2022 2746 2776 3105 513 Size: 552 957 724 30 329 3931 Fragments arranged by size: 3931 957 724 552 329 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5784 Fragments arranged by size: 5784 683 56 BtsI GCAGTG_nn' Cuts at: 2674 2874 4039 4833 4853 2674 Size: 200 1165 794 20 4344 Fragments arranged by size: 4344 1165 794 200 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6523 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6488 DraI TTT'AAA Cuts at: 3827 3827 Size: 6523 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5530 809 Size: 507 4214 1802 Fragments arranged by size: 4214 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2325 3102 3663 Size: 54 1425 50 275 18 495 777 561 Cuts at: 3663 3970 4859 6488 8 Size: 307 889 1629 43 Fragments arranged by size: 1629 1425 889 777 561 495 307 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3970 1537 Size: 275 2158 4090 Fragments arranged by size: 4090 2158 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4453 1353 Size: 3100 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5110 5484 5630 387 Size: 1064 138 3521 374 146 1280 Fragments arranged by size: 3521 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3550 3658 3739 3550 Size: 108 81 6334 Fragments arranged by size: 6334 108 81 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2141 2329 2888 3035 3647 4586 Size: 393 482 27 188 559 147 612 939 Cuts at: 4586 5183 5970 6238 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 939 787 612 597 559 482 393 268 188 147 27 EcoNI CCTnn'n_nnAGG Cuts at: 2731 3467 3541 2731 Size: 736 74 5713 Fragments arranged by size: 5713 736 74 EcoO109I rG'GnC_Cy Cuts at: 2181 2362 2512 2908 2181 Size: 181 150 396 5796 Fragments arranged by size: 5796 396 181 150 EcoRI G'AATT_C Cuts at: 3832 4210 3832 Size: 378 6145 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2401 2401 Size: 6523 FspI TGC'GCA Cuts at: 3266 3488 5029 3266 Size: 222 1541 4760 Fragments arranged by size: 4760 1541 222 FspAI rTGC'GCAy Cuts at: 3266 3266 Size: 6523 HaeII r_GCGC'y Cuts at: 1678 2101 2770 3341 3442 5670 1678 Size: 423 669 571 101 2228 2531 Fragments arranged by size: 2531 2228 669 571 423 101 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2584 2616 3103 3135 5164 Size: 32 135 32 1012 32 487 32 2029 Cuts at: 5164 5196 5238 5270 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2029 1012 487 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3867 4113 669 Size: 988 281 1929 246 3079 Fragments arranged by size: 3079 1929 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6523 HpaI GTT'AAC Cuts at: 4113 4113 Size: 6523 KpnI G_GTAC'C Cuts at: 3848 3848 Size: 6523 MfeI C'AATT_G Cuts at: 4122 4122 Size: 6523 MluI A'CGCG_T Cuts at: 6399 6399 Size: 6523 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2434 3315 5637 5821 1305 Size: 1129 881 2322 184 2007 Fragments arranged by size: 2322 2007 1129 881 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3665 10 Size: 54 1425 343 1833 2868 Fragments arranged by size: 2868 1833 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2829 3209 4481 4840 4999 518 Size: 648 1663 380 1272 359 159 2042 Fragments arranged by size: 2042 1663 1272 648 380 359 159 MspA1I CmG'CkG Cuts at: 1606 2210 2227 2238 3314 3637 3753 3939 4616 Size: 604 17 11 1076 323 116 186 677 Cuts at: 4616 5764 6009 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1076 677 604 323 245 186 116 17 11 NaeI GCC'GGC Cuts at: 3620 3620 Size: 6523 NcoI C'CATG_G Cuts at: 513 1065 2022 2746 2776 513 Size: 552 957 724 30 4260 Fragments arranged by size: 4260 957 724 552 30 NdeI CA'TA_TG Cuts at: 387 387 Size: 6523 NgoMIV G'CCGG_C Cuts at: 3618 3618 Size: 6523 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6523 NotI GC'GGCC_GC Cuts at: 3970 3970 Size: 6523 NspI r_CATG'y Cuts at: 1132 1229 2305 3169 3388 3881 5426 1132 Size: 97 1076 864 219 493 1545 2229 Fragments arranged by size: 2229 1545 1076 864 493 219 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6523 PciI A'CATG_T Cuts at: 5422 5422 Size: 6523 PflMI CCAn_nnn'nTGG Cuts at: 1108 2554 1108 Size: 1446 5077 PmeI GTTT'AAAC Cuts at: 3827 3827 Size: 6523 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4592 4624 6105 6137 4592 Size: 32 1481 32 4978 Fragments arranged by size: 4978 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2181 2362 2512 2181 Size: 181 150 6192 Fragments arranged by size: 6192 181 150 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6523 PsiI TTA'TAA Cuts at: 4093 4093 Size: 6523 PspXI vC'TCGA_Gb Cuts at: 2903 2903 Size: 6523 PstI C_TGCA'G Cuts at: 830 2041 2219 2370 2881 3393 3423 3609 3875 Size: 1211 178 151 511 512 30 186 266 Cuts at: 3875 830 Size: 3478 Fragments arranged by size: 3478 1211 512 511 266 186 178 151 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6523 PvuII CAG'CTG Cuts at: 2238 3637 3753 2238 Size: 1399 116 5008 Fragments arranged by size: 5008 1399 116 SacI G_AGCT'C Cuts at: 3552 3660 3741 3552 Size: 108 81 6334 Fragments arranged by size: 6334 108 81 SalI G'TCGA_C Cuts at: 1936 3865 1936 Size: 1929 4594 SbfI CC_TGCA'GG Cuts at: 3875 3875 Size: 6523 ScaI AGT'ACT Cuts at: 3785 4771 6483 3785 Size: 986 1712 3825 Fragments arranged by size: 3825 1712 986 SexAI A'CCwGG_T Cuts at: 2127 2127 Size: 6523 SfcI C'TryA_G Cuts at: 826 1045 2037 2215 2366 2752 2877 3389 3419 Size: 219 992 178 151 386 125 512 30 Cuts at: 3419 3605 3871 5006 5687 5878 826 Size: 186 266 1135 681 191 1471 Fragments arranged by size: 1471 1135 992 681 512 386 266 219 191 186 178 151 125 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6523 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6523 SmaI CCC'GGG Cuts at: 1872 3850 1872 Size: 1978 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2903 4627 5528 5790 820 Size: 197 926 960 1724 901 262 1553 Fragments arranged by size: 1724 1553 960 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3820 4314 493 Size: 3327 494 2702 Fragments arranged by size: 3327 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6523 SphI G_CATG'C Cuts at: 1132 3169 3881 1132 Size: 2037 712 3774 Fragments arranged by size: 3774 2037 712 SspI AAT'ATT Cuts at: 5 52 4447 5 Size: 47 4395 2081 Fragments arranged by size: 4395 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6523 StyI C'CwwG_G Cuts at: 513 1065 2022 2746 2776 3962 513 Size: 552 957 724 30 1186 3074 Fragments arranged by size: 3074 1186 957 724 552 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2405 2808 4550 4567 4720 4905 5350 Size: 1255 825 403 1742 17 153 185 445 Cuts at: 5350 325 Size: 1498 Fragments arranged by size: 1742 1498 1255 825 445 403 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3611 3783 4769 Size: 274 80 33 51 1443 1634 172 986 Cuts at: 4769 6481 96 Size: 1712 138 Fragments arranged by size: 1712 1634 1443 986 274 172 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2681 2713 6483 6515 2681 Size: 32 3770 32 2689 Fragments arranged by size: 3770 2689 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2185 3712 1316 Size: 869 1527 4127 Fragments arranged by size: 4127 1527 869 XbaI T'CTAG_A Cuts at: 3859 3859 Size: 6523 XhoI C'TCGA_G Cuts at: 1943 2903 1943 Size: 960 5563 XmaI C'CCGG_G Cuts at: 1870 3848 1870 Size: 1978 4545 XmnI GAAnn'nnTTC Cuts at: 2579 3398 4652 2579 Size: 819 1254 4450 Fragments arranged by size: 4450 1254 819 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4331 276 Size: 53 83 186 936 2797 2468 Fragments arranged by size: 2797 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoRV FseI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI