Restriction map for
pFN21ASDA0420
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# NO CUT ENZYMES ( 98 enzymes) #
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AatII AccIII AclI AcsI AflII
AflIII AgeI AocI Aor51HI ApoI
AscI AseI AsnI AviII AvrII
BalI BamHI BbvCI BciVI BfrI
BglI BglII BlnI BsaBI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 520 GGTACC G^GTACC
Alw44I 154 GTGCAC G^TGCAC
AlwNI 1128 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 154 GTGCAC G^TGCAC
Asp700I 72 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 520 GGTACC G^GTACC
AspI 1894 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 1301 TGATCA T^GATCA
BfaI 1147 CTAG C^TAG
BlpI 2025 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2025 GCTCAGC GC^T[ACGT]AGC
BsiEI 1183 CGGCCG CG[GA][CT]^CG
BspHI 819 TCATGA T^CATGA
BsrFI 176 GCCGGC [GA]^CCGG[CT]
BssSI 1567 CACGAG CACGAG(-5/-1)
CelII 2025 GCTCAGC GC^T[ACGT]AGC
Cfr10I 176 GCCGGC [GA]^CCGG[CT]
EagI 1183 CGGCCG C^GGCCG
EarI 1486 GAAGAG CTCTTC(1/4)
EclXI 1183 CGGCCG C^GGCCG
Eco52I 1183 CGGCCG C^GGCCG
FbaI 1301 TGATCA T^GATCA
HincII 1069 GTCAAC GT[CT]^[GA]AC
HindII 1069 GTCAAC GT[CT]^[GA]AC
KpnI 520 GGTACC GGTAC^C
Ksp632I 1486 GAAGAG CTCTTC(1/4)
MaeI 1147 CTAG C^TAG
MslI 714 CACGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 176 GCCGGC GCC^GGC
NgoMIV 176 GCCGGC G^CCGGC
PflFI 1894 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1566 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 819 TCATGA T^CATGA
SacI 505 GAGCTC GAGCT^C
SexAI 884 ACCAGGT A^CC[AT]GGT
SmlI 864 CTTGAG C^T[CT][GA]AG
StuI 1377 AGGCCT AGG^CCT
Tsp45I 1810 GTGAC ^GT[GC]AC
Tsp509I 1231 AATT ^AATT
Tth111I 1894 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1566 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 72 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccI 31 GTCTAC GT^[AC][GT]AC
1828 GTCTAC
AhdI 397 GACATTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1242 GACCCTGGGTC
AspEI 397 GACATTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1242 GACCCTGGGTC
BbeI 1668 GGCGCC GGCGC^C
1681 GGCGCC
BcgI 378 CGAGCAGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
795 GCACATCCTTCG
BsrBI 641 CCGCTC CCGCTC(-3/-3)
166 GAGCGG
Eam1105I 397 GACATTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1242 GACCCTGGGTC
EcoNI 831 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
924 CCTGCTGGAGG
EcoT14I 428 CCTTGG C^C[AT][AT]GG
1419 CCTTGG
KasI 1668 GGCGCC G^GCGCC
1681 GGCGCC
KspI 196 CCGCGG CCGC^GG
1554 CCGCGG
MaeIII 756 GTTAC ^GT[ACGT]AC
1810 GTGAC
NarI 1668 GGCGCC GG^CGCC
1681 GGCGCC
NspI 1056 GCATGT [GA]CATG^[CT]
1166 ACATGC
PstI 333 CTGCAG CTGCA^G
1875 CTGCAG
SacII 196 CCGCGG CCGC^GG
1554 CCGCGG
SfoI 1668 GGCGCC GGC^GCC
1681 GGCGCC
StyI 428 CCTTGG C^C[AT][AT]GG
1419 CCTTGG
TfiI 780 GATTC G^A[AT]TC
1999 GAATC
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BanI BanII BbrPI BbsI BbvI
BcnI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PmaCI PmlI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI TaqI TseI TspRI TthHB8I
XcmI XhoII XmaCI XmaI