(Circular) (Six-base) MAPSORT of: pFN21ASDA0420.seq Check: 2960 from: 1 to: 6817 pFN21ASDA0420 6817 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2231 2231 Size: 6817 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4627 278 Size: 53 83 186 936 3091 2468 Fragments arranged by size: 3091 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2055 3852 4160 1937 Size: 118 1797 308 4594 Fragments arranged by size: 4594 1797 308 118 Acc65I G'GTAC_C Cuts at: 2543 4138 2543 Size: 1595 5222 AclI AA'CG_TT Cuts at: 1563 4944 5317 1563 Size: 3381 373 3063 Fragments arranged by size: 3381 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2909 3982 4880 6264 6532 1632 Size: 1277 1073 898 1384 268 1917 Fragments arranged by size: 1917 1384 1277 1073 898 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6620 AflIII A'CryG_T Cuts at: 1203 5716 6693 1203 Size: 4513 977 1327 Fragments arranged by size: 4513 1327 977 AgeI A'CCGG_T Cuts at: 5648 5648 Size: 6817 AhdI GACnn_n'nnGTC Cuts at: 2425 3270 5546 2425 Size: 845 2276 3696 Fragments arranged by size: 3696 2276 845 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6817 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2958 1184 Size: 1774 5043 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3650 3682 3650 Size: 32 6785 AlwNI CAG_nnn'CTG Cuts at: 3156 4560 6132 3156 Size: 1404 1572 3841 Fragments arranged by size: 3841 1572 1404 ApaI G_GGCC'C Cuts at: 2646 2664 3951 2646 Size: 18 1287 5512 Fragments arranged by size: 5512 1287 18 ApaLI G'TGCA_C Cuts at: 2177 4874 6030 2177 Size: 2697 1156 2964 Fragments arranged by size: 2964 2697 1156 ApoI r'AATT_y Cuts at: 1493 4126 4352 4504 6613 1493 Size: 2633 226 152 2109 1697 Fragments arranged by size: 2633 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5371 160 Size: 872 4339 1606 Fragments arranged by size: 4339 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6817 AvaI C'yCGr_G Cuts at: 1870 1943 2865 3297 3708 3907 3944 4142 1870 Size: 73 922 432 411 199 37 198 4545 Fragments arranged by size: 4545 922 432 411 199 198 73 37 BamHI G'GATC_C Cuts at: 4147 4514 4147 Size: 367 6450 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2543 2721 3195 3691 3704 Size: 325 217 627 756 178 474 496 13 Cuts at: 3704 4138 5593 618 Size: 434 1455 1842 Fragments arranged by size: 1842 1455 756 627 496 474 434 325 217 178 13 BanII G_rGCy'C Cuts at: 1391 2532 2646 2664 2842 3295 3586 3827 3951 Size: 1141 114 18 178 453 291 241 124 Cuts at: 3951 4007 4136 1391 Size: 56 129 4072 Fragments arranged by size: 4072 1141 453 291 241 178 129 124 114 56 18 BbeI G_GCGC'C Cuts at: 3695 3708 3695 Size: 13 6804 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2119 2251 2391 3295 928 Size: 971 220 132 140 904 4450 Fragments arranged by size: 4450 971 904 220 140 132 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2390 2424 2807 2841 5008 5042 1903 Size: 34 453 34 383 34 2167 34 3678 Fragments arranged by size: 3678 2167 453 383 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4709 5919 4709 Size: 1210 5607 BclI T'GATC_A Cuts at: 1548 3324 1548 Size: 1776 5041 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5428 136 Size: 107 122 71 631 605 3756 1525 Fragments arranged by size: 3756 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6812 6812 Size: 6817 BlpI GC'TnA_GC Cuts at: 4049 4234 4049 Size: 185 6632 Bme1580I G_kGCm'C Cuts at: 1790 2114 2181 2646 2664 3951 4878 6034 1790 Size: 324 67 465 18 1287 927 1156 2573 Fragments arranged by size: 2573 1287 1156 927 465 324 67 18 BmgBI CAC'GTC Cuts at: 1318 3661 1318 Size: 2343 4474 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2117 2570 4548 Size: 416 53 83 399 24 700 453 1978 Cuts at: 4548 5506 442 Size: 958 1753 Fragments arranged by size: 1978 1753 958 700 453 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6817 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2551 2551 Size: 6817 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2651 2690 2972 3197 3845 4090 5477 1239 Size: 1412 39 282 225 648 245 1387 2579 Fragments arranged by size: 2579 1412 1387 648 282 245 225 39 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2908 4942 5807 6105 2908 Size: 2034 865 298 3620 Fragments arranged by size: 3620 2034 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2315 2780 3257 3545 4108 5480 882 Size: 617 816 465 477 288 563 1372 2219 Fragments arranged by size: 2219 1372 816 617 563 477 465 288 BsaAI yAC'GTr Cuts at: 493 1204 2109 2182 2254 4114 4608 493 Size: 711 905 73 72 1860 494 2702 Fragments arranged by size: 2702 1860 905 711 494 73 72 BsaBI GATnn'nnATC Cuts at: 6811 6811 Size: 6817 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2214 2567 3692 3705 Size: 53 83 186 936 681 353 1125 13 Cuts at: 3705 3849 4624 5006 5641 275 Size: 144 775 382 635 1451 Fragments arranged by size: 1451 1125 936 775 681 635 382 353 186 144 83 53 13 BsaWI w'CCGG_w Cuts at: 5250 5648 5922 6069 6480 5250 Size: 398 274 147 411 5587 Fragments arranged by size: 5587 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2609 2639 3390 3420 3441 Size: 30 165 30 1206 30 751 30 21 Cuts at: 3441 3468 3471 3498 3609 3639 1178 Size: 27 3 27 111 30 4356 Fragments arranged by size: 4356 1206 751 165 111 30 30 30 30 30 27 27 21 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2291 2600 2909 2969 3077 3527 4060 4063 Size: 1105 309 309 60 108 450 533 3 Cuts at: 4063 1186 Size: 3940 Fragments arranged by size: 3940 1105 533 450 309 309 108 60 3 BseYI C'CCAG_C Cuts at: 2621 3136 3700 3753 3884 4044 6020 6516 2621 Size: 515 564 53 131 160 1976 496 2922 Fragments arranged by size: 2922 1976 564 515 496 160 131 53 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2285 3920 4012 1163 Size: 573 549 1635 92 3968 Fragments arranged by size: 3968 1635 573 549 92 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3209 4267 5028 6056 1540 Size: 275 186 1208 1058 761 1028 2301 Fragments arranged by size: 2301 1208 1058 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2181 2253 2355 2532 2820 3315 3525 3576 3984 Size: 72 102 177 288 495 210 51 408 Cuts at: 3984 4878 4963 6034 2181 Size: 894 85 1071 2964 Fragments arranged by size: 2964 1071 894 495 408 288 210 177 102 85 72 51 BsmI GAATG_Cn' Cuts at: 4328 4421 4328 Size: 93 6724 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2114 2181 2253 2355 2532 2646 2664 Size: 399 324 67 72 102 177 114 18 Cuts at: 2664 2820 2842 3295 3315 3525 3576 3586 3827 Size: 156 22 453 20 210 51 10 241 Cuts at: 3827 3951 3984 4007 4136 4878 4963 6034 1391 Size: 124 33 23 129 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 453 399 324 241 210 177 156 129 124 114 102 85 72 67 51 33 23 22 20 18 10 BspEI T'CCGG_A Cuts at: 6480 6480 Size: 6817 BspHI T'CATG_A Cuts at: 2842 4706 6436 2842 Size: 1864 1730 3223 Fragments arranged by size: 3223 1864 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2231 3170 3864 4172 844 Size: 1387 939 694 308 3489 Fragments arranged by size: 3489 1387 939 694 308 BsrBI CCG'CTC Cuts at: 1394 1823 2191 2666 4704 1394 Size: 429 368 475 2038 3507 Fragments arranged by size: 3507 2038 475 429 368 BsrDI GCAATG_nn' Cuts at: 65 1248 5312 5486 65 Size: 1183 4064 174 1396 Fragments arranged by size: 4064 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2199 5461 5648 1700 Size: 110 389 3262 187 2869 Fragments arranged by size: 3262 2869 389 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6817 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6817 BssSI C'ACGA_G Cuts at: 3590 4877 5889 3590 Size: 1287 1012 4518 Fragments arranged by size: 4518 1287 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6817 BstBI TT'CG_AA Cuts at: 2007 5621 2007 Size: 3614 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6817 BstXI CCAn_nnnn'nTGG Cuts at: 2338 2629 3892 2338 Size: 291 1263 5263 Fragments arranged by size: 5263 1263 291 BstYI r'GATC_y Cuts at: 1972 2118 2914 3606 4147 4514 4899 4916 6368 Size: 146 796 692 541 367 385 17 1452 Cuts at: 6368 6454 6466 6812 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 796 692 541 385 367 346 146 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2219 2638 3054 3577 3600 513 Size: 552 957 197 419 416 523 23 3730 Fragments arranged by size: 3730 957 552 523 419 416 197 23 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6078 Fragments arranged by size: 6078 683 56 BtsI GCAGTG_nn' Cuts at: 2353 3548 4333 5127 5147 2353 Size: 1195 785 794 20 4023 Fragments arranged by size: 4023 1195 794 785 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6817 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6782 DraI TTT'AAA Cuts at: 4121 4121 Size: 6817 DraIII CAC_nnn'GTG Cuts at: 2185 2911 3595 2185 Size: 726 684 5407 Fragments arranged by size: 5407 726 684 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5824 809 Size: 507 4508 1802 Fragments arranged by size: 4508 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2995 3206 3597 Size: 54 1425 50 275 18 1165 211 391 Cuts at: 3597 4078 4264 5153 6782 8 Size: 481 186 889 1629 43 Fragments arranged by size: 1629 1425 1165 889 481 391 275 211 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3206 4264 1537 Size: 275 1394 1058 4090 Fragments arranged by size: 4090 1394 1058 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3504 4747 1353 Size: 2151 1243 3423 Fragments arranged by size: 3423 2151 1243 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3005 3370 5404 5778 5924 387 Size: 1064 138 1416 365 2034 374 146 1280 Fragments arranged by size: 2034 1416 1280 1064 374 365 146 138 EcoICRI GAG'CTC Cuts at: 2530 2530 Size: 6817 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2651 2690 2909 2972 3197 3845 3982 Size: 393 1019 39 219 63 225 648 137 Cuts at: 3982 4090 4880 5477 6264 6532 1239 Size: 108 790 597 787 268 1524 Fragments arranged by size: 1524 1019 790 787 648 597 393 268 225 219 137 108 63 39 EcoNI CCTnn'n_nnAGG Cuts at: 2858 2951 2858 Size: 93 6724 EcoO109I rG'GnC_Cy Cuts at: 2617 2643 2660 3220 3392 3757 2617 Size: 26 17 560 172 365 5677 Fragments arranged by size: 5677 560 365 172 26 17 EcoRI G'AATT_C Cuts at: 4126 4504 4126 Size: 378 6439 FspI TGC'GCA Cuts at: 5323 5323 Size: 6817 HaeII r_GCGC'y Cuts at: 1678 3071 3695 3708 4056 5964 1678 Size: 1393 624 13 348 1908 2531 Fragments arranged by size: 2531 1908 1393 624 348 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2411 2443 2609 2641 3256 Size: 32 135 32 839 32 166 32 615 Cuts at: 3256 3288 3414 3446 3468 3500 3609 3641 5458 Size: 32 126 32 22 32 109 32 1817 Cuts at: 5458 5490 5532 5564 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1817 839 615 166 135 126 109 42 32 32 32 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 3094 4161 4407 669 Size: 988 281 1156 1067 246 3079 Fragments arranged by size: 3079 1156 1067 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6817 HpaI GTT'AAC Cuts at: 4407 4407 Size: 6817 KasI G'GCGC_C Cuts at: 3691 3704 3691 Size: 13 6804 KpnI G_GTAC'C Cuts at: 2547 4142 2547 Size: 1595 5222 MfeI C'AATT_G Cuts at: 4416 4416 Size: 6817 MluI A'CGCG_T Cuts at: 6693 6693 Size: 6817 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5931 6115 1305 Size: 4626 184 2007 Fragments arranged by size: 4626 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4995 Fragments arranged by size: 4995 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2741 4775 5134 5293 518 Size: 648 1575 2034 359 159 2042 Fragments arranged by size: 2042 2034 1575 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2221 3136 3535 3579 3732 3739 4035 4233 Size: 615 915 399 44 153 7 296 198 Cuts at: 4233 4910 6058 6303 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 915 677 615 399 296 245 198 153 44 7 NaeI GCC'GGC Cuts at: 2201 2201 Size: 6817 NarI GG'CG_CC Cuts at: 3692 3705 3692 Size: 13 6804 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5308 Fragments arranged by size: 5308 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6817 NgoMIV G'CCGG_C Cuts at: 2199 2199 Size: 6817 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6817 NotI GC'GGCC_GC Cuts at: 4264 4264 Size: 6817 NspI r_CATG'y Cuts at: 1132 1229 3083 3193 4175 5720 1132 Size: 97 1854 110 982 1545 2229 Fragments arranged by size: 2229 1854 1545 982 110 97 PasI CC'CwG_GG Cuts at: 1348 2539 2656 3268 1348 Size: 1191 117 612 4897 Fragments arranged by size: 4897 1191 612 117 PciI A'CATG_T Cuts at: 5716 5716 Size: 6817 PflMI CCAn_nnn'nTGG Cuts at: 1108 3595 1108 Size: 2487 4330 PfoI T'CCnGG_A Cuts at: 3155 3609 3155 Size: 454 6363 PmeI GTTT'AAAC Cuts at: 4121 4121 Size: 6817 PmlI CAC'GTG Cuts at: 2109 2182 2254 2109 Size: 73 72 6672 Fragments arranged by size: 6672 73 72 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4886 4918 6399 6431 4886 Size: 32 1481 32 5272 Fragments arranged by size: 5272 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2617 3392 3757 2617 Size: 775 365 5677 Fragments arranged by size: 5677 775 365 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6817 PsiI TTA'TAA Cuts at: 4387 4387 Size: 6817 PspOMI G'GGCC_C Cuts at: 2642 2660 3947 2642 Size: 18 1287 5512 Fragments arranged by size: 5512 1287 18 PstI C_TGCA'G Cuts at: 830 2360 3902 4169 830 Size: 1530 1542 267 3478 Fragments arranged by size: 3478 1542 1530 267 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6817 PvuII CAG'CTG Cuts at: 3136 3535 3739 3136 Size: 399 204 6214 Fragments arranged by size: 6214 399 204 SacI G_AGCT'C Cuts at: 2532 2532 Size: 6817 SacII CC_GC'GG Cuts at: 2222 3580 2222 Size: 1358 5459 SalI G'TCGA_C Cuts at: 1936 4159 1936 Size: 2223 4594 SanDI GG'GwC_CC Cuts at: 2617 3392 2617 Size: 775 6042 SbfI CC_TGCA'GG Cuts at: 4169 4169 Size: 6817 ScaI AGT'ACT Cuts at: 5065 6777 5065 Size: 1712 5105 SexAI A'CCwGG_T Cuts at: 2907 2907 Size: 6817 SfcI C'TryA_G Cuts at: 826 1045 2356 3370 3898 4165 5300 5981 6172 Size: 219 1311 1014 528 267 1135 681 191 Cuts at: 6172 826 Size: 1471 Fragments arranged by size: 1471 1311 1135 1014 681 528 267 219 191 SfoI GGC'GCC Cuts at: 3693 3706 3693 Size: 13 6804 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6817 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6817 SmaI CCC'GGG Cuts at: 1872 2867 3710 3909 3946 4144 1872 Size: 995 843 199 37 198 4545 Fragments arranged by size: 4545 995 843 199 198 37 SmlI C'TyrA_G Cuts at: 820 1017 1943 2887 4921 5822 6084 820 Size: 197 926 944 2034 901 262 1553 Fragments arranged by size: 2034 1553 944 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4114 4608 493 Size: 3621 494 2702 Fragments arranged by size: 3621 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6817 SphI G_CATG'C Cuts at: 1132 4175 1132 Size: 3043 3774 SspI AAT'ATT Cuts at: 5 52 4741 5 Size: 47 4689 2081 Fragments arranged by size: 4689 2081 47 StuI AGG'CCT Cuts at: 1517 3402 1517 Size: 1885 4932 StyI C'CwwG_G Cuts at: 513 1065 2022 2451 3442 4256 513 Size: 552 957 429 991 814 3074 Fragments arranged by size: 3074 991 957 814 552 429 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2415 2626 3613 4844 4861 5014 5199 Size: 1255 835 211 987 1231 17 153 185 Cuts at: 5199 5644 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1231 987 835 445 211 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2475 5063 6775 Size: 274 80 33 51 1443 498 2588 1712 Cuts at: 6775 96 Size: 138 Fragments arranged by size: 2588 1712 1443 498 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3390 3422 3441 3473 3521 3553 6777 6809 3390 Size: 32 19 32 48 32 3224 32 3398 Fragments arranged by size: 3398 3224 48 32 32 32 32 19 Tth111I GACn'n_nGTC Cuts at: 1316 3920 1316 Size: 2604 4213 XbaI T'CTAG_A Cuts at: 4153 4153 Size: 6817 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2338 2990 4015 2338 Size: 652 1025 5140 Fragments arranged by size: 5140 1025 652 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6817 XmaI C'CCGG_G Cuts at: 1870 2865 3708 3907 3944 4142 1870 Size: 995 843 199 37 198 4545 Fragments arranged by size: 4545 995 843 199 198 37 XmnI GAAnn'nnTTC Cuts at: 2099 4946 2099 Size: 2847 3970 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4625 276 Size: 53 83 186 936 3091 2468 Fragments arranged by size: 3091 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SapI SfiI SrfI SwaI