Restriction map for
pFN21ASDA0420
(Linear) (Six-base) MAPSORT of: KIAA0420.seq Check: 5320 from: 1 to: 2088
KIAA0420.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 208 2088
Size: 208 1880
AccI GT'mk_AC
Cuts at: 0 32 1829 2088
Size: 32 1797 259
Fragments arranged by size:
1797 259 32
Acc65I G'GTAC_C
Cuts at: 0 520 2088
Size: 520 1568
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 1959 2088
Size: 886 1073 129
Fragments arranged by size:
1073 886 129
AhdI GACnn_n'nnGTC
Cuts at: 0 402 1247 2088
Size: 402 845 841
Fragments arranged by size:
845 841 402
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 935 2088
Size: 935 1153
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1627 1659 2088
Size: 1627 32 429
Fragments arranged by size:
1627 429 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1133 2088
Size: 1133 955
ApaI G_GGCC'C
Cuts at: 0 623 641 1928 2088
Size: 623 18 1287 160
Fragments arranged by size:
1287 623 160 18
ApaLI G'TGCA_C
Cuts at: 0 154 2088
Size: 154 1934
AvaI C'yCGr_G
Cuts at: 0 842 1274 1685 1884 1921 2088
Size: 842 432 411 199 37 167
Fragments arranged by size:
842 432 411 199 167 37
BanI G'GyrC_C
Cuts at: 0 520 698 1172 1668 1681 2088
Size: 520 178 474 496 13 407
Fragments arranged by size:
520 496 474 407 178 13
BanII G_rGCy'C
Cuts at: 0 509 623 641 819 1272 1563 1804 1928
Size: 509 114 18 178 453 291 241 124
Cuts at: 1928 1984 2088
Size: 56 104
Fragments arranged by size:
509 453 291 241 178 124 114 104
56 18
BbeI G_GCGC'C
Cuts at: 0 1672 1685 2088
Size: 1672 13 403
Fragments arranged by size:
1672 403 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 96 228 368 1272 2088
Size: 96 132 140 904 816
Fragments arranged by size:
904 816 140 132 96
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 367 401 784 818 2088
Size: 367 34 383 34 1270
Fragments arranged by size:
1270 383 367 34 34
BclI T'GATC_A
Cuts at: 0 1301 2088
Size: 1301 787
BlpI GC'TnA_GC
Cuts at: 0 2026 2088
Size: 2026 62
Bme1580I G_kGCm'C
Cuts at: 0 91 158 623 641 1928 2088
Size: 91 67 465 18 1287 160
Fragments arranged by size:
1287 465 160 91 67 18
BmgBI CAC'GTC
Cuts at: 0 1638 2088
Size: 1638 450
BmrI ACTGGGnnnn_n'
Cuts at: 0 94 547 2088
Size: 94 453 1541
Fragments arranged by size:
1541 453 94
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 528 2088
Size: 528 1560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 667 949 1174 1822 2067 2088
Size: 628 39 282 225 648 245 21
Fragments arranged by size:
648 628 282 245 225 39 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 885 2088
Size: 885 1203
BsaI GGTCTCn'nnnn_
Cuts at: 0 292 757 1234 1522 2085 2088
Size: 292 465 477 288 563 3
Fragments arranged by size:
563 477 465 292 288 3
BsaAI yAC'GTr
Cuts at: 0 86 159 231 2088
Size: 86 73 72 1857
Fragments arranged by size:
1857 86 73 72
BsaHI Gr'CG_yC
Cuts at: 0 191 544 1669 1682 1826 2088
Size: 191 353 1125 13 144 262
Fragments arranged by size:
1125 353 262 191 144 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 586 616 1367 1397 1418 1445 1448 1475
Size: 586 30 751 30 21 27 3 27
Cuts at: 1475 1586 1616 2088
Size: 111 30 472
Fragments arranged by size:
751 586 472 111 30 30 30 27
27 21 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 268 577 886 946 1054 1504 2037 2040
Size: 268 309 309 60 108 450 533 3
Cuts at: 2040 2088
Size: 48
Fragments arranged by size:
533 450 309 309 268 108 60 48
3
BseYI C'CCAG_C
Cuts at: 0 598 1113 1677 1730 1861 2021 2088
Size: 598 515 564 53 131 160 67
Fragments arranged by size:
598 564 515 160 131 67 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 1897 1989 2088
Size: 262 1635 92 99
Fragments arranged by size:
1635 262 99 92
BsiEI CG_ry'CG
Cuts at: 0 1186 2088
Size: 1186 902
BsiHKAI G_wGCw'C
Cuts at: 0 158 230 332 509 797 1292 1502 1553
Size: 158 72 102 177 288 495 210 51
Cuts at: 1553 1961 2088
Size: 408 127
Fragments arranged by size:
495 408 288 210 177 158 127 102
72 51
Bsp1286I G_dGCh'C
Cuts at: 0 91 158 230 332 509 623 641 797
Size: 91 67 72 102 177 114 18 156
Cuts at: 797 819 1272 1292 1502 1553 1563 1804 1928
Size: 22 453 20 210 51 10 241 124
Cuts at: 1928 1961 1984 2088
Size: 33 23 104
Fragments arranged by size:
453 241 210 177 156 124 114 104
102 91 72 67 51 33 23 22
20 18 10
BspHI T'CATG_A
Cuts at: 0 819 2088
Size: 819 1269
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 208 1147 1841 2088
Size: 208 939 694 247
Fragments arranged by size:
939 694 247 208
BsrBI CCG'CTC
Cuts at: 0 168 643 2088
Size: 168 475 1445
Fragments arranged by size:
1445 475 168
BsrFI r'CCGG_y
Cuts at: 0 176 2088
Size: 176 1912
BssSI C'ACGA_G
Cuts at: 0 1567 2088
Size: 1567 521
BstXI CCAn_nnnn'nTGG
Cuts at: 0 315 606 1869 2088
Size: 315 291 1263 219
Fragments arranged by size:
1263 315 291 219
BstYI r'GATC_y
Cuts at: 0 95 891 1583 2088
Size: 95 796 692 505
Fragments arranged by size:
796 692 505 95
BtgI C'CryG_G
Cuts at: 0 196 615 1031 1554 1577 2088
Size: 196 419 416 523 23 511
Fragments arranged by size:
523 511 419 416 196 23
BtsI GCAGTG_nn'
Cuts at: 0 330 1525 2088
Size: 330 1195 563
Fragments arranged by size:
1195 563 330
DraIII CAC_nnn'GTG
Cuts at: 0 162 888 1572 2088
Size: 162 726 684 516
Fragments arranged by size:
726 684 516 162
EaeI y'GGCC_r
Cuts at: 0 972 1183 1574 2055 2088
Size: 972 211 391 481 33
Fragments arranged by size:
972 481 391 211 33
EagI C'GGCC_G
Cuts at: 0 1183 2088
Size: 1183 905
EarI CTCTTCn'nnn_
Cuts at: 0 1481 2088
Size: 1481 607
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 982 1347 2088
Size: 982 365 741
Fragments arranged by size:
982 741 365
EcoICRI GAG'CTC
Cuts at: 0 507 2088
Size: 507 1581
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 667 886 949 1174 1822 1959 2067
Size: 628 39 219 63 225 648 137 108
Cuts at: 2067 2088
Size: 21
Fragments arranged by size:
648 628 225 219 137 108 63 39
21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 835 928 2088
Size: 835 93 1160
Fragments arranged by size:
1160 835 93
EcoO109I rG'GnC_Cy
Cuts at: 0 594 620 637 1197 1369 1734 2088
Size: 594 26 17 560 172 365 354
Fragments arranged by size:
594 560 365 354 172 26 17
HaeII r_GCGC'y
Cuts at: 0 1048 1672 1685 2033 2088
Size: 1048 624 13 348 55
Fragments arranged by size:
1048 624 348 55 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 388 420 586 618 1233 1265 1391 1423
Size: 388 32 166 32 615 32 126 32
Cuts at: 1423 1445 1477 1586 1618 2088
Size: 22 32 109 32 470
Fragments arranged by size:
615 470 388 166 126 109 32 32
32 32 32 32 22
HincII GTy'rAC
Cuts at: 0 1071 2088
Size: 1071 1017
KasI G'GCGC_C
Cuts at: 0 1668 1681 2088
Size: 1668 13 407
Fragments arranged by size:
1668 407 13
KpnI G_GTAC'C
Cuts at: 0 524 2088
Size: 524 1564
MslI CAynn'nnrTG
Cuts at: 0 718 2088
Size: 718 1370
MspA1I CmG'CkG
Cuts at: 0 198 1113 1512 1556 1709 1716 2012 2088
Size: 198 915 399 44 153 7 296 76
Fragments arranged by size:
915 399 296 198 153 76 44 7
NaeI GCC'GGC
Cuts at: 0 178 2088
Size: 178 1910
NarI GG'CG_CC
Cuts at: 0 1669 1682 2088
Size: 1669 13 406
Fragments arranged by size:
1669 406 13
NgoMIV G'CCGG_C
Cuts at: 0 176 2088
Size: 176 1912
NspI r_CATG'y
Cuts at: 0 1060 1170 2088
Size: 1060 110 918
Fragments arranged by size:
1060 918 110
PasI CC'CwG_GG
Cuts at: 0 516 633 1245 2088
Size: 516 117 612 843
Fragments arranged by size:
843 612 516 117
PflMI CCAn_nnn'nTGG
Cuts at: 0 1572 2088
Size: 1572 516
PfoI T'CCnGG_A
Cuts at: 0 1132 1586 2088
Size: 1132 454 502
Fragments arranged by size:
1132 502 454
PmlI CAC'GTG
Cuts at: 0 86 159 231 2088
Size: 86 73 72 1857
Fragments arranged by size:
1857 86 73 72
PpuMI rG'GwC_Cy
Cuts at: 0 594 1369 1734 2088
Size: 594 775 365 354
Fragments arranged by size:
775 594 365 354
PspOMI G'GGCC_C
Cuts at: 0 619 637 1924 2088
Size: 619 18 1287 164
Fragments arranged by size:
1287 619 164 18
PstI C_TGCA'G
Cuts at: 0 337 1879 2088
Size: 337 1542 209
Fragments arranged by size:
1542 337 209
PvuII CAG'CTG
Cuts at: 0 1113 1512 1716 2088
Size: 1113 399 204 372
Fragments arranged by size:
1113 399 372 204
SacI G_AGCT'C
Cuts at: 0 509 2088
Size: 509 1579
SacII CC_GC'GG
Cuts at: 0 199 1557 2088
Size: 199 1358 531
Fragments arranged by size:
1358 531 199
SanDI GG'GwC_CC
Cuts at: 0 594 1369 2088
Size: 594 775 719
Fragments arranged by size:
775 719 594
SexAI A'CCwGG_T
Cuts at: 0 884 2088
Size: 884 1204
SfcI C'TryA_G
Cuts at: 0 333 1347 1875 2088
Size: 333 1014 528 213
Fragments arranged by size:
1014 528 333 213
SfoI GGC'GCC
Cuts at: 0 1670 1683 2088
Size: 1670 13 405
Fragments arranged by size:
1670 405 13
SmaI CCC'GGG
Cuts at: 0 844 1687 1886 1923 2088
Size: 844 843 199 37 165
Fragments arranged by size:
844 843 199 165 37
SmlI C'TyrA_G
Cuts at: 0 864 2088
Size: 864 1224
StuI AGG'CCT
Cuts at: 0 1379 2088
Size: 1379 709
StyI C'CwwG_G
Cuts at: 0 428 1419 2088
Size: 428 991 669
Fragments arranged by size:
991 669 428
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 392 603 1590 2088
Size: 392 211 987 498
Fragments arranged by size:
987 498 392 211
TatI w'GTAC_w
Cuts at: 0 452 2088
Size: 452 1636
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1367 1399 1418 1450 1498 1530 2088
Size: 1367 32 19 32 48 32 558
Fragments arranged by size:
1367 558 48 32 32 32 19
Tth111I GACn'n_nGTC
Cuts at: 0 1897 2088
Size: 1897 191
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 315 967 1992 2088
Size: 315 652 1025 96
Fragments arranged by size:
1025 652 315 96
XmaI C'CCGG_G
Cuts at: 0 842 1685 1884 1921 2088
Size: 842 843 199 37 167
Fragments arranged by size:
843 842 199 167 37
XmnI GAAnn'nnTTC
Cuts at: 0 76 2088
Size: 76 2012
Enzymes that do cut:
AarI AccI Acc65I AcuI AhdI AlfI AloI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BbsI BcgI
BclI BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstXI
BstYI BtgI BtsI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI
KpnI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI
SacII SanDI SexAI SfcI SfoI SmaI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AleI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI
BfrBI BglI BglII BmtI Bpu10I BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
MfeI MluI MmeI MscI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XhoI ZraI