(Circular) (Six-base) MAPSORT of: pFN21ASDA0414.seq Check: 7931 from: 1 to: 6130 pFN21ASDA0414 6130 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2953 2953 Size: 6130 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2127 2299 3940 278 Size: 53 83 186 936 591 172 1641 2468 Fragments arranged by size: 2468 1641 936 591 186 172 83 53 AccI GT'mk_AC Cuts at: 1937 2370 3473 1937 Size: 433 1103 4594 Fragments arranged by size: 4594 1103 433 Acc65I G'GTAC_C Cuts at: 3451 3451 Size: 6130 AclI AA'CG_TT Cuts at: 1563 4257 4630 1563 Size: 2694 373 3063 Fragments arranged by size: 3063 2694 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2403 4193 5577 5845 1632 Size: 771 1790 1384 268 1917 Fragments arranged by size: 1917 1790 1384 771 268 AfeI AGC'GCT Cuts at: 2771 2771 Size: 6130 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5933 AflIII A'CryG_T Cuts at: 1203 5029 6006 1203 Size: 3826 977 1327 Fragments arranged by size: 3826 1327 977 AgeI A'CCGG_T Cuts at: 4961 4961 Size: 6130 AhdI GACnn_n'nnGTC Cuts at: 2443 2575 4859 2443 Size: 132 2284 3714 Fragments arranged by size: 3714 2284 132 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6130 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2816 1184 Size: 1632 4498 AlwNI CAG_nnn'CTG Cuts at: 2089 3128 3873 5445 2089 Size: 1039 745 1572 2774 Fragments arranged by size: 2774 1572 1039 745 ApaLI G'TGCA_C Cuts at: 2801 4187 5343 2801 Size: 1386 1156 3588 Fragments arranged by size: 3588 1386 1156 ApoI r'AATT_y Cuts at: 1493 2054 3245 3282 3439 3665 3817 5926 1493 Size: 561 1191 37 157 226 152 2109 1697 Fragments arranged by size: 2109 1697 1191 561 226 157 152 37 AseI AT'TA_AT Cuts at: 160 1032 4684 160 Size: 872 3652 1606 Fragments arranged by size: 3652 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 2765 3455 1870 Size: 73 822 690 4545 Fragments arranged by size: 4545 822 690 73 BamHI G'GATC_C Cuts at: 2751 3460 3827 2751 Size: 709 367 5054 Fragments arranged by size: 5054 709 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2675 3451 4906 618 Size: 325 217 627 888 776 1455 1842 Fragments arranged by size: 1842 1455 888 776 627 325 217 BanII G_rGCy'C Cuts at: 1391 2498 3449 1391 Size: 1107 951 4072 Fragments arranged by size: 4072 1107 951 BbeI G_GCGC'C Cuts at: 2679 2679 Size: 6130 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3203 928 Size: 971 1304 3855 Fragments arranged by size: 3855 1304 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3187 3221 4321 4355 1903 Size: 34 1250 34 1100 34 3678 Fragments arranged by size: 3678 1250 1100 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3150 4022 5232 3150 Size: 872 1210 4048 Fragments arranged by size: 4048 1210 872 BclI T'GATC_A Cuts at: 1548 1548 Size: 6130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4741 136 Size: 107 122 71 631 605 3069 1525 Fragments arranged by size: 3069 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6125 6125 Size: 6130 BlpI GC'TnA_GC Cuts at: 3399 3547 3399 Size: 148 5982 Bme1580I G_kGCm'C Cuts at: 1790 2161 2805 3033 4191 5347 1790 Size: 371 644 228 1158 1156 2573 Fragments arranged by size: 2573 1158 1156 644 371 228 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6130 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3861 4819 442 Size: 416 53 83 399 24 2444 958 1753 Fragments arranged by size: 2444 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6130 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2512 2512 Size: 6130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2298 2336 4790 1239 Size: 1059 38 2454 2579 Fragments arranged by size: 2579 2454 1059 38 Bpu10I CC'TnA_GC Cuts at: 2639 2639 Size: 6130 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4255 5120 5418 4255 Size: 865 298 4967 Fragments arranged by size: 4967 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4793 882 Size: 617 3294 2219 Fragments arranged by size: 3294 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2758 3427 3921 493 Size: 711 1554 669 494 2702 Fragments arranged by size: 2702 1554 711 669 494 BsaBI GATnn'nnATC Cuts at: 3018 6124 3018 Size: 3106 3024 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2124 2296 2676 3937 Size: 53 83 186 936 591 172 380 1261 Cuts at: 3937 4319 4954 275 Size: 382 635 1451 Fragments arranged by size: 1451 1261 936 635 591 382 380 186 172 83 53 BsaWI w'CCGG_w Cuts at: 2999 4563 4961 5235 5382 5793 2999 Size: 1564 398 274 147 411 3336 Fragments arranged by size: 3336 1564 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3331 3361 1178 Size: 30 165 30 1928 30 3947 Fragments arranged by size: 3947 1928 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2570 2684 2900 1186 Size: 1384 114 216 4416 Fragments arranged by size: 4416 1384 216 114 BseYI C'CCAG_C Cuts at: 2491 2583 2609 2726 5333 5829 2491 Size: 92 26 117 2607 496 2792 Fragments arranged by size: 2792 2607 496 117 92 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2870 2888 1163 Size: 573 1134 18 4405 Fragments arranged by size: 4405 1134 573 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3580 4341 5369 1540 Size: 275 186 1579 761 1028 2301 Fragments arranged by size: 2301 1579 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2564 2598 2645 2805 2823 2862 4191 4276 5347 Size: 34 47 160 18 39 1329 85 1071 Cuts at: 5347 2564 Size: 3347 Fragments arranged by size: 3347 1329 1071 160 85 47 39 34 18 BsmI GAATG_Cn' Cuts at: 3641 3734 3641 Size: 93 6037 BsmBI CGTCTCn'nnnn_ Cuts at: 2131 2131 Size: 6130 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2161 2498 2564 2598 2645 2805 2823 Size: 399 371 337 66 34 47 160 18 Cuts at: 2823 2862 3033 3449 4191 4276 5347 1391 Size: 39 171 416 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 416 399 371 337 171 160 85 66 47 39 34 18 BspEI T'CCGG_A Cuts at: 2999 5793 2999 Size: 2794 3336 BspHI T'CATG_A Cuts at: 4019 5749 4019 Size: 1730 4400 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2613 2953 3047 3485 844 Size: 1769 340 94 438 3489 Fragments arranged by size: 3489 1769 438 340 94 BsrBI CCG'CTC Cuts at: 1394 1823 2312 4017 1394 Size: 429 489 1705 3507 Fragments arranged by size: 3507 1705 489 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4625 4799 65 Size: 1183 3377 174 1396 Fragments arranged by size: 3377 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3354 4774 4961 1700 Size: 110 1544 1420 187 2869 Fragments arranged by size: 2869 1544 1420 187 110 BsrGI T'GTAC_A Cuts at: 96 2715 96 Size: 2619 3511 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6130 BssSI C'ACGA_G Cuts at: 3266 4190 5202 3266 Size: 924 1012 4194 Fragments arranged by size: 4194 1012 924 BstAPI GCAn_nnn'nTGC Cuts at: 794 2866 794 Size: 2072 4058 BstBI TT'CG_AA Cuts at: 2007 4934 2007 Size: 2927 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6130 BstXI CCAn_nnnn'nTGG Cuts at: 2734 2734 Size: 6130 BstYI r'GATC_y Cuts at: 1972 2751 3460 3827 4212 4229 5681 5767 5779 Size: 779 709 367 385 17 1452 86 12 Cuts at: 5779 6125 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 779 709 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2985 513 Size: 552 957 963 3658 Fragments arranged by size: 3658 963 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5391 Fragments arranged by size: 5391 683 56 BtsI GCAGTG_nn' Cuts at: 2440 3646 4440 4460 2440 Size: 1206 794 20 4110 Fragments arranged by size: 4110 1206 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6130 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6095 DraI TTT'AAA Cuts at: 3434 3434 Size: 6130 DraIII CAC_nnn'GTG Cuts at: 2866 2866 Size: 6130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5137 809 Size: 507 3821 1802 Fragments arranged by size: 3821 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3577 4466 6095 Size: 54 1425 50 275 18 1747 889 1629 Cuts at: 6095 8 Size: 43 Fragments arranged by size: 1747 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3577 1537 Size: 275 1765 4090 Fragments arranged by size: 4090 1765 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2547 2985 2986 4060 1353 Size: 1194 438 1 1074 3423 Fragments arranged by size: 3423 1194 1074 438 1 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4717 5091 5237 387 Size: 1064 138 3128 374 146 1280 Fragments arranged by size: 3128 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2298 2336 2403 4193 4790 5577 5845 Size: 393 666 38 67 1790 597 787 268 Cuts at: 5845 1239 Size: 1524 Fragments arranged by size: 1790 1524 787 666 597 393 268 67 38 EcoRI G'AATT_C Cuts at: 3439 3817 3439 Size: 378 5752 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2189 2189 Size: 6130 FspI TGC'GCA Cuts at: 4636 4636 Size: 6130 HaeII r_GCGC'y Cuts at: 1678 2104 2679 2691 2773 5277 1678 Size: 426 575 12 82 2504 2531 Fragments arranged by size: 2531 2504 575 426 82 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2429 2461 2561 2564 2593 Size: 32 135 32 857 32 100 3 29 Cuts at: 2593 2596 4771 4803 4845 4877 1373 Size: 3 2175 32 42 32 2626 Fragments arranged by size: 2626 2175 857 135 100 42 32 32 32 32 32 29 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 3474 3720 669 Size: 988 281 1536 246 3079 Fragments arranged by size: 3079 1536 988 281 246 HindIII A'AGCT_T Cuts at: 748 2344 3102 748 Size: 1596 758 3776 Fragments arranged by size: 3776 1596 758 HpaI GTT'AAC Cuts at: 3720 3720 Size: 6130 KasI G'GCGC_C Cuts at: 2675 2675 Size: 6130 KpnI G_GTAC'C Cuts at: 3455 3455 Size: 6130 MfeI C'AATT_G Cuts at: 3729 3729 Size: 6130 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5244 5428 1305 Size: 3939 184 2007 Fragments arranged by size: 3939 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4308 Fragments arranged by size: 4308 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2433 2648 2798 3197 3357 4088 4447 Size: 648 1267 215 150 399 160 731 359 Cuts at: 4447 4606 518 Size: 159 2042 Fragments arranged by size: 2042 1267 731 648 399 359 215 160 159 150 MspA1I CmG'CkG Cuts at: 1606 2339 3546 4223 5371 5616 1606 Size: 733 1207 677 1148 245 2120 Fragments arranged by size: 2120 1207 1148 733 677 245 NarI GG'CG_CC Cuts at: 2676 2676 Size: 6130 NcoI C'CATG_G Cuts at: 513 1065 2022 2985 513 Size: 552 957 963 3658 Fragments arranged by size: 3658 963 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6130 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6130 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 2366 3202 3362 3488 5033 1132 Size: 97 1137 836 160 126 1545 2229 Fragments arranged by size: 2229 1545 1137 836 160 126 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6130 PciI A'CATG_T Cuts at: 5029 5029 Size: 6130 PflMI CCAn_nnn'nTGG Cuts at: 1108 3268 1108 Size: 2160 3970 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PmlI CAC'GTG Cuts at: 2758 2758 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4199 4231 5712 5744 4199 Size: 32 1481 32 4585 Fragments arranged by size: 4585 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6130 PsiI TTA'TAA Cuts at: 2466 3700 2466 Size: 1234 4896 PstI C_TGCA'G Cuts at: 830 3482 830 Size: 2652 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6130 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SapI GCTCTTCn'nnn_ Cuts at: 2986 2986 Size: 6130 SbfI CC_TGCA'GG Cuts at: 3482 3482 Size: 6130 ScaI AGT'ACT Cuts at: 4378 6090 4378 Size: 1712 4418 SexAI A'CCwGG_T Cuts at: 2202 2823 2202 Size: 621 5509 SfcI C'TryA_G Cuts at: 826 1045 2081 2403 3478 4613 5294 5485 826 Size: 219 1036 322 1075 1135 681 191 1471 Fragments arranged by size: 1471 1135 1075 1036 681 322 219 191 SfoI GGC'GCC Cuts at: 2677 2677 Size: 6130 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 3457 1872 Size: 1585 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4234 5135 5397 820 Size: 197 926 2291 901 262 1553 Fragments arranged by size: 2291 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3427 3921 493 Size: 2934 494 2702 Fragments arranged by size: 2934 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 3202 3488 1132 Size: 2070 286 3774 Fragments arranged by size: 3774 2070 286 SspI AAT'ATT Cuts at: 5 52 3073 4054 5 Size: 47 3021 981 2081 Fragments arranged by size: 3021 2081 981 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6130 StyI C'CwwG_G Cuts at: 513 1065 2022 2140 2985 3569 513 Size: 552 957 118 845 584 3074 Fragments arranged by size: 3074 957 845 584 552 118 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3197 4157 4174 4327 4512 4957 325 Size: 1255 1617 960 17 153 185 445 1498 Fragments arranged by size: 1617 1498 1255 960 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2715 3371 4376 Size: 274 80 33 51 1443 738 656 1005 Cuts at: 4376 6088 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1005 738 656 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6090 6122 6090 Size: 32 6098 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6130 XbaI T'CTAG_A Cuts at: 3466 3466 Size: 6130 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6130 XmaI C'CCGG_G Cuts at: 1870 3455 1870 Size: 1585 4545 XmnI GAAnn'nnTTC Cuts at: 3161 4259 3161 Size: 1098 5032 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2125 2297 3938 276 Size: 53 83 186 936 591 172 1641 2468 Fragments arranged by size: 2468 1641 936 591 186 172 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI