Restriction map for
pFN21ASDA0390
(Linear) (Six-base) MAPSORT of: KIAA0390.seq Check: 674 from: 1 to: 3900
KIAA0390.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 536 3900
Size: 536 3364
Acc65I G'GTAC_C
Cuts at: 0 1286 3900
Size: 1286 2614
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 2532 3443 3900
Size: 691 1841 911 457
Fragments arranged by size:
1841 911 691 457
AfeI AGC'GCT
Cuts at: 0 412 1394 2216 3900
Size: 412 982 822 1684
Fragments arranged by size:
1684 982 822 412
AflII C'TTAA_G
Cuts at: 0 2296 3900
Size: 2296 1604
AflIII A'CryG_T
Cuts at: 0 1697 3900
Size: 1697 2203
AhdI GACnn_n'nnGTC
Cuts at: 0 2034 3900
Size: 2034 1866
AleI CACnn'nnGTG
Cuts at: 0 62 1759 1949 2321 3900
Size: 62 1697 190 372 1579
Fragments arranged by size:
1697 1579 372 190 62
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 667 1541 3900
Size: 667 874 2359
Fragments arranged by size:
2359 874 667
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1141 1173 3900
Size: 1141 32 2727
Fragments arranged by size:
2727 1141 32
AlwNI CAG_nnn'CTG
Cuts at: 0 105 330 3386 3660 3771 3900
Size: 105 225 3056 274 111 129
Fragments arranged by size:
3056 274 225 129 111 105
ApaI G_GGCC'C
Cuts at: 0 1000 3764 3900
Size: 1000 2764 136
Fragments arranged by size:
2764 1000 136
ApaLI G'TGCA_C
Cuts at: 0 903 1942 2302 3900
Size: 903 1039 360 1598
Fragments arranged by size:
1598 1039 903 360
ApoI r'AATT_y
Cuts at: 0 2736 3861 3900
Size: 2736 1125 39
Fragments arranged by size:
2736 1125 39
AvaI C'yCGr_G
Cuts at: 0 142 166 1225 1846 2019 2607 2961 3134
Size: 142 24 1059 621 173 588 354 173
Cuts at: 3134 3900
Size: 766
Fragments arranged by size:
1059 766 621 588 354 173 173 142
24
BanI G'GyrC_C
Cuts at: 0 762 1230 1286 3637 3900
Size: 762 468 56 2351 263
Fragments arranged by size:
2351 762 468 263 56
BanII G_rGCy'C
Cuts at: 0 56 149 944 1000 1154 1346 2071 2242
Size: 56 93 795 56 154 192 725 171
Cuts at: 2242 2968 3044 3764 3900
Size: 726 76 720 136
Fragments arranged by size:
795 726 725 720 192 171 154 136
93 76 56 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 23 1652 2621 3703 3900
Size: 23 1629 969 1082 197
Fragments arranged by size:
1629 1082 969 197 23
BbvCI CC'TCA_GC
Cuts at: 0 3589 3900
Size: 3589 311
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3771 3805 3900
Size: 3771 34 95
Fragments arranged by size:
3771 95 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2306 3900
Size: 2306 1594
BclI T'GATC_A
Cuts at: 0 3203 3900
Size: 3203 697
BglI GCCn_nnn'nGGC
Cuts at: 0 694 703 727 811 982 1004 3139 3900
Size: 694 9 24 84 171 22 2135 761
Fragments arranged by size:
2135 761 694 171 84 24 22 9
BglII A'GATC_T
Cuts at: 0 1127 2191 3900
Size: 1127 1064 1709
Fragments arranged by size:
1709 1127 1064
BlpI GC'TnA_GC
Cuts at: 0 50 127 3825 3900
Size: 50 77 3698 75
Fragments arranged by size:
3698 77 75 50
Bme1580I G_kGCm'C
Cuts at: 0 212 397 481 767 907 1000 1241 1900
Size: 212 185 84 286 140 93 241 659
Cuts at: 1900 1946 2130 2306 3764 3900
Size: 46 184 176 1458 136
Fragments arranged by size:
1458 659 286 241 212 185 184 176
140 136 93 84 46
BmgBI CAC'GTC
Cuts at: 0 750 3900
Size: 750 3150
BmrI ACTGGGnnnn_n'
Cuts at: 0 2034 2870 3900
Size: 2034 836 1030
Fragments arranged by size:
2034 1030 836
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2125 2658 3900
Size: 2125 533 1242
Fragments arranged by size:
2125 1242 533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 625 1266 1404 2618 3043 3363 3900
Size: 211 414 641 138 1214 425 320 537
Fragments arranged by size:
1214 641 537 425 414 320 211 138
Bpu10I CC'TnA_GC
Cuts at: 0 1336 3369 3589 3900
Size: 1336 2033 220 311
Fragments arranged by size:
2033 1336 311 220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 1063 1147 3235 3900
Size: 502 561 84 2088 665
Fragments arranged by size:
2088 665 561 502 84
BsaI GGTCTCn'nnnn_
Cuts at: 0 1769 2536 3669 3900
Size: 1769 767 1133 231
Fragments arranged by size:
1769 1133 767 231
BsaAI yAC'GTr
Cuts at: 0 207 954 1698 1995 3297 3900
Size: 207 747 744 297 1302 603
Fragments arranged by size:
1302 747 744 603 297 207
BsaHI Gr'CG_yC
Cuts at: 0 368 1436 2201 3900
Size: 368 1068 765 1699
Fragments arranged by size:
1699 1068 765 368
BsaWI w'CCGG_w
Cuts at: 0 2279 3558 3684 3900
Size: 2279 1279 126 216
Fragments arranged by size:
2279 1279 216 126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1090 1120 1141 1171 1264 1294 1441 1471
Size: 1090 30 21 30 93 30 147 30
Cuts at: 1471 1684 1714 2026 2056 3478 3496 3508 3526
Size: 213 30 312 30 1422 18 12 18
Cuts at: 3526 3679 3709 3900
Size: 153 30 191
Fragments arranged by size:
1422 1090 312 213 191 153 147 93
30 30 30 30 30 30 30 21
18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 208 595 622 874 949 952 1993
Size: 184 24 387 27 252 75 3 1041
Cuts at: 1993 2061 2101 2142 2154 2194 2567 2583 2881
Size: 68 40 41 12 40 373 16 298
Cuts at: 2881 2958 3486 3504 3900
Size: 77 528 18 396
Fragments arranged by size:
1041 528 396 387 373 298 252 184
77 75 68 41 40 40 27 24
18 16 12 3
BseYI C'CCAG_C
Cuts at: 0 122 261 543 689 1216 1345 1395 2185
Size: 122 139 282 146 527 129 50 790
Cuts at: 2185 2425 3644 3900
Size: 240 1219 256
Fragments arranged by size:
1219 790 527 282 256 240 146 139
129 122 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 424 580 696 808 1975 3001 3900
Size: 91 333 156 116 112 1167 1026 899
Fragments arranged by size:
1167 1026 899 333 156 116 112 91
BsiEI CG_ry'CG
Cuts at: 0 664 977 1137 1911 3900
Size: 664 313 160 774 1989
Fragments arranged by size:
1989 774 664 313 160
BsiHKAI G_wGCw'C
Cuts at: 0 149 907 944 1118 1946 2306 3044 3900
Size: 149 758 37 174 828 360 738 856
Fragments arranged by size:
856 828 758 738 360 174 149 37
Bsp1286I G_dGCh'C
Cuts at: 0 56 149 212 397 481 767 907 944
Size: 56 93 63 185 84 286 140 37
Cuts at: 944 1000 1118 1154 1241 1346 1900 1946 2071
Size: 56 118 36 87 105 554 46 125
Cuts at: 2071 2130 2242 2306 2968 3044 3764 3900
Size: 59 112 64 662 76 720 136
Fragments arranged by size:
720 662 554 286 185 140 136 125
118 112 105 93 87 84 76 64
63 59 56 56 46 37 36
BspEI T'CCGG_A
Cuts at: 0 2279 3900
Size: 2279 1621
BspHI T'CATG_A
Cuts at: 0 1313 3900
Size: 1313 2587
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 536 1787 1865 3900
Size: 536 1251 78 2035
Fragments arranged by size:
2035 1251 536 78
BsrBI CCG'CTC
Cuts at: 0 218 1512 2010 2054 2093 2412 3278 3756
Size: 218 1294 498 44 39 319 866 478
Cuts at: 3756 3900
Size: 144
Fragments arranged by size:
1294 866 498 478 319 218 144 44
39
BsrDI GCAATG_nn'
Cuts at: 0 2990 3488 3900
Size: 2990 498 412
Fragments arranged by size:
2990 498 412
BsrFI r'CCGG_y
Cuts at: 0 1136 1330 2197 2359 3289 3900
Size: 1136 194 867 162 930 611
Fragments arranged by size:
1136 930 867 611 194 162
BssHII G'CGCG_C
Cuts at: 0 612 3900
Size: 612 3288
BssSI C'ACGA_G
Cuts at: 0 598 1480 3456 3900
Size: 598 882 1976 444
Fragments arranged by size:
1976 882 598 444
BstAPI GCAn_nnn'nTGC
Cuts at: 0 911 1629 1731 3900
Size: 911 718 102 2169
Fragments arranged by size:
2169 911 718 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 64 261 1357 1590 3072 3900
Size: 64 197 1096 233 1482 828
Fragments arranged by size:
1482 1096 828 233 197 64
BstYI r'GATC_y
Cuts at: 0 1127 2191 3900
Size: 1127 1064 1709
Fragments arranged by size:
1709 1127 1064
Bsu36I CC'TnA_GG
Cuts at: 0 2308 2512 3802 3900
Size: 2308 204 1290 98
Fragments arranged by size:
2308 1290 204 98
BtgI C'CryG_G
Cuts at: 0 227 1241 1431 1595 2618 2847 3035 3900
Size: 227 1014 190 164 1023 229 188 865
Fragments arranged by size:
1023 1014 865 229 227 190 188 164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3623 3900
Size: 3623 277
BtsI GCAGTG_nn'
Cuts at: 0 1116 2031 2578 3700 3900
Size: 1116 915 547 1122 200
Fragments arranged by size:
1122 1116 915 547 200
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 546 581 990 1025 3900
Size: 546 35 409 35 2875
Fragments arranged by size:
2875 546 409 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 762 911 1895 1935 2853 3900
Size: 762 149 984 40 918 1047
Fragments arranged by size:
1047 984 918 762 149 40
DrdI GACnn_nn'nnGTC
Cuts at: 0 2773 3900
Size: 2773 1127
EaeI y'GGCC_r
Cuts at: 0 81 219 251 661 704 974 1022 1134
Size: 81 138 32 410 43 270 48 112
Cuts at: 1134 1580 1592 1908 2243 2941 3291 3900
Size: 446 12 316 335 698 350 609
Fragments arranged by size:
698 609 446 410 350 335 316 270
138 112 81 48 43 32 12
EagI C'GGCC_G
Cuts at: 0 661 974 1134 1908 3900
Size: 661 313 160 774 1992
Fragments arranged by size:
1992 774 661 313 160
EarI CTCTTCn'nnn_
Cuts at: 0 2697 3268 3419 3533 3900
Size: 2697 571 151 114 367
Fragments arranged by size:
2697 571 367 151 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 52 3900
Size: 52 3848
EcoICRI GAG'CTC
Cuts at: 0 147 942 3042 3900
Size: 147 795 2100 858
Fragments arranged by size:
2100 858 795 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 625 691 1266 1404 2532 2618 3043
Size: 211 414 66 575 138 1128 86 425
Cuts at: 3043 3363 3443 3900
Size: 320 80 457
Fragments arranged by size:
1128 575 457 425 414 320 211 138
86 80 66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 556 2892 3900
Size: 556 2336 1008
Fragments arranged by size:
2336 1008 556
EcoO109I rG'GnC_Cy
Cuts at: 0 696 996 997 1864 2519 2557 2637 2747
Size: 696 300 1 867 655 38 80 110
Cuts at: 2747 3374 3726 3760 3761 3806 3900
Size: 627 352 34 1 45 94
Fragments arranged by size:
867 696 655 627 352 300 110 94
80 45 38 34 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2501 3900
Size: 2501 1399
FspI TGC'GCA
Cuts at: 0 448 1093 3900
Size: 448 645 2807
Fragments arranged by size:
2807 645 448
FspAI rTGC'GCAy
Cuts at: 0 448 3900
Size: 448 3452
HaeII r_GCGC'y
Cuts at: 0 247 414 1143 1396 2218 3900
Size: 247 167 729 253 822 1682
Fragments arranged by size:
1682 822 729 253 247 167
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1441 1473 1807 1839 2020 2026 2052 2058
Size: 1441 32 334 32 181 6 26 6
Cuts at: 2058 2274 2306 2458 2490 2938 2970 3195 3227
Size: 216 32 152 32 448 32 225 32
Cuts at: 3227 3466 3478 3496 3498 3510 3528 3900
Size: 239 12 18 2 12 18 372
Fragments arranged by size:
1441 448 372 334 239 225 216 181
152 32 32 32 32 32 32 26
18 18 12 12 6 6 2
HincII GTy'rAC
Cuts at: 0 3117 3900
Size: 3117 783
HindIII A'AGCT_T
Cuts at: 0 2483 2756 3900
Size: 2483 273 1144
Fragments arranged by size:
2483 1144 273
KpnI G_GTAC'C
Cuts at: 0 1290 3900
Size: 1290 2610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2079 3900
Size: 2079 1821
MscI TGG'CCA
Cuts at: 0 253 1582 1594 3900
Size: 253 1329 12 2306
Fragments arranged by size:
2306 1329 253 12
MslI CAynn'nnrTG
Cuts at: 0 62 1594 1759 1949 2321 2465 2623 3900
Size: 62 1532 165 190 372 144 158 1277
Fragments arranged by size:
1532 1277 372 190 165 158 144 62
MspA1I CmG'CkG
Cuts at: 0 261 636 702 824 1037 1491 1502 1629
Size: 261 375 66 122 213 454 11 127
Cuts at: 1629 2433 3900
Size: 804 1467
Fragments arranged by size:
1467 804 454 375 261 213 127 122
66 11
NaeI GCC'GGC
Cuts at: 0 1138 1332 2199 2361 3900
Size: 1138 194 867 162 1539
Fragments arranged by size:
1539 1138 867 194 162
NcoI C'CATG_G
Cuts at: 0 227 1241 1595 3035 3900
Size: 227 1014 354 1440 865
Fragments arranged by size:
1440 1014 865 354 227
NgoMIV G'CCGG_C
Cuts at: 0 1136 1330 2197 2359 3900
Size: 1136 194 867 162 1541
Fragments arranged by size:
1541 1136 867 194 162
NruI TCG'CGA
Cuts at: 0 2225 3900
Size: 2225 1675
NspI r_CATG'y
Cuts at: 0 447 1092 1254 2628 3792 3900
Size: 447 645 162 1374 1164 108
Fragments arranged by size:
1374 1164 645 447 162 108
PasI CC'CwG_GG
Cuts at: 0 987 3900
Size: 987 2913
PflMI CCAn_nnn'nTGG
Cuts at: 0 228 543 1935 2853 3660 3771 3900
Size: 228 315 1392 918 807 111 129
Fragments arranged by size:
1392 918 807 315 228 129 111
PfoI T'CCnGG_A
Cuts at: 0 1439 3900
Size: 1439 2461
PmlI CAC'GTG
Cuts at: 0 207 954 1995 3297 3900
Size: 207 747 1041 1302 603
Fragments arranged by size:
1302 1041 747 603 207
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1141 1173 3900
Size: 1141 32 2727
Fragments arranged by size:
2727 1141 32
PpuMI rG'GwC_Cy
Cuts at: 0 1864 2637 2747 3726 3900
Size: 1864 773 110 979 174
Fragments arranged by size:
1864 979 773 174 110
PspOMI G'GGCC_C
Cuts at: 0 996 3760 3900
Size: 996 2764 140
Fragments arranged by size:
2764 996 140
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 370 402 3900
Size: 370 32 3498
Fragments arranged by size:
3498 370 32
PstI C_TGCA'G
Cuts at: 0 656 728 1304 1520 1634 1862 3629 3900
Size: 656 72 576 216 114 228 1767 271
Fragments arranged by size:
1767 656 576 271 228 216 114 72
PvuII CAG'CTG
Cuts at: 0 261 636 1502 3900
Size: 261 375 866 2398
Fragments arranged by size:
2398 866 375 261
SacI G_AGCT'C
Cuts at: 0 149 944 3044 3900
Size: 149 795 2100 856
Fragments arranged by size:
2100 856 795 149
SapI GCTCTTCn'nnn_
Cuts at: 0 2697 3268 3900
Size: 2697 571 632
Fragments arranged by size:
2697 632 571
SbfI CC_TGCA'GG
Cuts at: 0 728 1862 3900
Size: 728 1134 2038
Fragments arranged by size:
2038 1134 728
SexAI A'CCwGG_T
Cuts at: 0 1931 3900
Size: 1931 1969
SfcI C'TryA_G
Cuts at: 0 652 724 1300 1516 1630 1858 3625 3810
Size: 652 72 576 216 114 228 1767 185
Cuts at: 3810 3900
Size: 90
Fragments arranged by size:
1767 652 576 228 216 185 114 90
72
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 703 982 3900
Size: 703 279 2918
Fragments arranged by size:
2918 703 279
SmaI CCC'GGG
Cuts at: 0 2963 3136 3900
Size: 2963 173 764
Fragments arranged by size:
2963 764 173
SmlI C'TyrA_G
Cuts at: 0 517 1078 1162 1846 2296 3250 3900
Size: 517 561 84 684 450 954 650
Fragments arranged by size:
954 684 650 561 517 450 84
StyI C'CwwG_G
Cuts at: 0 21 227 1241 1529 1595 2742 3035 3240
Size: 21 206 1014 288 66 1147 293 205
Cuts at: 3240 3518 3731 3900
Size: 278 213 169
Fragments arranged by size:
1147 1014 293 288 278 213 206 205
169 66 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2698 3786 3900
Size: 2698 1088 114
Fragments arranged by size:
2698 1088 114
TatI w'GTAC_w
Cuts at: 0 1277 3900
Size: 1277 2623
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1090 1122 2652 2684 3679 3711 3900
Size: 1090 32 1530 32 995 32 189
Fragments arranged by size:
1530 1090 995 189 32 32 32
XbaI T'CTAG_A
Cuts at: 0 3213 3900
Size: 3213 687
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1422 2854 3042 3900
Size: 1422 1432 188 858
Fragments arranged by size:
1432 1422 858 188
XhoI C'TCGA_G
Cuts at: 0 1846 3900
Size: 1846 2054
XmaI C'CCGG_G
Cuts at: 0 2961 3134 3900
Size: 2961 173 766
Fragments arranged by size:
2961 766 173
XmnI GAAnn'nnTTC
Cuts at: 0 469 2275 3900
Size: 469 1806 1625
Fragments arranged by size:
1806 1625 469
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI
HaeII Hin4I HincII HindIII KpnI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NruI NspI PasI PflMI
PfoI PmlI PpiI PpuMI PspOMI PsrI PstI PvuII
SacI SapI SbfI SexAI SfcI SfiI SmaI SmlI
StyI TaqII TatI TstI XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AccI AclI AgeI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BfrBI BmtI BsaBI BsiWI BsmI
BsmBI BsrGI BstBI BstEII BstZ17I ClaI DraI EcoRI
EcoRV FseI HpaI KasI MfeI MluI NarI NdeI
NheI NotI NsiI PacI PciI PmeI PshAI PsiI
PspXI PvuI RsrII SacII SalI SanDI ScaI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I ZraI