Restriction map for
pFN21ASDA0388
##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BbeI BbrPI
BcgI BciVI BlnI BlpI Bpu1102I
BsaWI BseAI BsiEI BsiWI BsmI
BspEI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HincII HindII HpaI KasI KpnI
KspI MluI MluNI MroI MscI
NarI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 2233 GACGTC GACGT^C
AccII 1963 CGCG CG^CG
AcyI 2233 GACGTC G[GA]^CG[CT]C
AflII 1831 CTTAAG C^TTAAG
AflIII 1985 ACATGT A^C[GA][CT]GT
Alw44I 816 GTGCAC G^TGCAC
ApaLI 816 GTGCAC G^TGCAC
AvaI 267 CCCGGG C^[CT]CG[GA]G
BamHI 499 GGATCC G^GATCC
BbsI 1330 GTCTTC GAAGAC(2/6)
BbvCI 1124 CCTCAGC CCTCAGC(-5/-2)
BclI 449 TGATCA T^GATCA
BfaI 1244 CTAG C^TAG
BfrI 1831 CTTAAG C^TTAAG
BglI 2137 GCCAAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 621 AGATCT A^GATCT
BpuAI 1330 GTCTTC GAAGAC(2/6)
BsaAI 2209 TACGTG [CT]AC^GT[GA]
BsaBI 2124 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2233 GACGTC G[GA]^CG[CT]C
BsmBI 376 GAGACG CGTCTC(1/5)
BsoBI 267 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1553 TGTACA T^GTACA
BspDI 829 ATCGAT AT^CGAT
BspLU11I 1985 ACATGT A^CATGT
BsrBI 1059 CCGCTC CCGCTC(-3/-3)
BsrFI 896 GCCGGC [GA]^CCGG[CT]
BsrGI 1553 TGTACA T^GTACA
BstUI 1963 CGCG CG^CG
CfoI 900 GCGC GCG^C
Cfr10I 896 GCCGGC [GA]^CCGG[CT]
ClaI 829 ATCGAT AT^CGAT
DraI 907 TTTAAA TTT^AAA
DraII 1768 GGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 1865 TGGCCG [CT]^GGCC[GA]
EcoO109I 1768 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1909 GAATTC G^AATTC
FbaI 449 TGATCA T^GATCA
HaeII 899 GGCGCT [GA]GCGC^[CT]
HgaI 1961 GACGC GACGC(5/10)
HhaI 900 GCGC GCG^C
Hin1I 2233 GACGTC G[GA]^CG[CT]C
HinP1I 900 GCGC G^CGC
HindIII 184 AAGCTT A^AGCTT
MaeI 1244 CTAG C^TAG
MamI 2124 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 716 CAATTG C^AATTG
MslI 1545 CACACAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 716 CAATTG C^AATTG
MvnI 1963 CGCG CG^CG
NaeI 896 GCCGGC GCC^GGC
NdeI 286 CATATG CA^TATG
NgoMIV 896 GCCGGC G^CCGGC
PvuII 1522 CAGCTG CAG^CTG
ScaI 554 AGTACT AGT^ACT
SmaI 267 CCCGGG CCC^GGG
SspBI 1553 TGTACA T^GTACA
SspI 391 AATATT AAT^ATT
XcmI 2093 CCAAAGTGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 267 CCCGGG C^CCGGG
XmaI 267 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 1127 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1596 CAGCACCTG
AvaII 341 GGTCC G^G[AT]CC
1382 GGACC
BcnI 267 CCCGG CC^[GC]GG
268 CCGGG
BpmI 1191 CTCCAG CTGGAG(16/14)
529 CTGGAG
BsaI 2114 GAGACC GGTCTC(1/5)
2227 GAGACC
BspHI 1457 TCATGA T^CATGA
1613 TCATGA
BsrDI 1178 GCAATG GCAATG(2/0)
1710 GCAATG
BstXI 1370 CCAGGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2118 CCATCGGATTGG
DsaI 474 CCATGG C^C[GA][CT]GG
1335 CCATGG
EcoT14I 474 CCATGG C^C[AT][AT]GG
1335 CCATGG
FauI 1058 CCCGC CCCGC(4/6)
858 GCGGG
MspA1I 856 CAGCGG C[AC]G^C[GT]G
1522 CAGCTG
NciI 267 CCCGG CC^[GC]GG
268 CCGGG
NcoI 474 CCATGG C^CATGG
1335 CCATGG
PshAI 1750 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2229 GACCGACGTC
PstI 1509 CTGCAG CTGCA^G
1812 CTGCAG
RcaI 1457 TCATGA T^CATGA
1613 TCATGA
SapI 2161 GAAGAGC GCTCTTC(1/4)
2166 GCTCTTC
SfcI 1509 CTGCAG C^T[GA][CT]AG
1812 CTGCAG
SmlI 1831 CTTAAG C^T[CT][GA]AG
2203 CTCAAG
StyI 474 CCATGG C^C[AT][AT]GG
1335 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI BanI BanII
BbvI BmyI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI