(Circular) (Six-base) MAPSORT of: pFN21ASDA0388.seq Check: 3735 from: 1 to: 6970 pFN21ASDA0388 6970 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3632 3873 3632 Size: 241 6729 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4260 4780 278 Size: 53 83 186 936 2724 520 2468 Fragments arranged by size: 2724 2468 936 520 186 83 53 AccI GT'mk_AC Cuts at: 1937 4313 1937 Size: 2376 4594 Acc65I G'GTAC_C Cuts at: 4291 4291 Size: 6970 AclI AA'CG_TT Cuts at: 1563 5097 5470 1563 Size: 3534 373 3063 Fragments arranged by size: 3534 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2102 2716 3207 3354 3479 3569 5033 6417 Size: 470 614 491 147 125 90 1464 1384 Cuts at: 6417 6685 1632 Size: 268 1917 Fragments arranged by size: 1917 1464 1384 614 491 470 268 147 125 90 AflII C'TTAA_G Cuts at: 820 1017 3854 820 Size: 197 2837 3936 Fragments arranged by size: 3936 2837 197 AflIII A'CryG_T Cuts at: 1203 4008 5869 6846 1203 Size: 2805 1861 977 1327 Fragments arranged by size: 2805 1861 1327 977 AgeI A'CCGG_T Cuts at: 5801 5801 Size: 6970 AhdI GACnn_n'nnGTC Cuts at: 5699 5699 Size: 6970 AleI CACnn'nnGTG Cuts at: 1166 3572 1166 Size: 2406 4564 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6970 AlwNI CAG_nnn'CTG Cuts at: 3155 3624 4713 6285 3155 Size: 469 1089 1572 3840 Fragments arranged by size: 3840 1572 1089 469 ApaLI G'TGCA_C Cuts at: 2839 5027 6183 2839 Size: 2188 1156 3626 Fragments arranged by size: 3626 2188 1156 ApoI r'AATT_y Cuts at: 1493 2155 3646 3932 4074 4279 4505 4657 6766 Size: 662 1491 286 142 205 226 152 2109 Cuts at: 6766 1493 Size: 1697 Fragments arranged by size: 2109 1697 1491 662 286 226 205 152 142 AseI AT'TA_AT Cuts at: 160 1032 5524 160 Size: 872 4492 1606 Fragments arranged by size: 4492 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6970 AvaI C'yCGr_G Cuts at: 1870 1943 2290 4295 1870 Size: 73 347 2005 4545 Fragments arranged by size: 4545 2005 347 73 BamHI G'GATC_C Cuts at: 2522 4300 4667 2522 Size: 1778 367 4825 Fragments arranged by size: 4825 1778 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3114 3362 3899 4291 5746 Size: 325 217 627 1327 248 537 392 1455 Cuts at: 5746 618 Size: 1842 Fragments arranged by size: 1842 1455 1327 627 537 392 325 248 217 BanII G_rGCy'C Cuts at: 1391 3480 3960 4191 4289 1391 Size: 2089 480 231 98 4072 Fragments arranged by size: 4072 2089 480 231 98 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3346 928 Size: 971 1447 4552 Fragments arranged by size: 4552 1447 971 BbvCI CC'TCA_GC Cuts at: 3148 3148 Size: 6970 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5161 5195 1903 Size: 34 3224 34 3678 Fragments arranged by size: 3678 3224 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4862 6072 4862 Size: 1210 5760 BclI T'GATC_A Cuts at: 1548 2472 1548 Size: 924 6046 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4166 5581 136 Size: 107 122 71 631 605 2494 1415 1525 Fragments arranged by size: 2494 1525 1415 631 605 122 107 71 BglII A'GATC_T Cuts at: 2644 6965 2644 Size: 4321 2649 BlpI GC'TnA_GC Cuts at: 4387 4387 Size: 6970 Bme1580I G_kGCm'C Cuts at: 1790 2604 2843 3902 5031 6187 1790 Size: 814 239 1059 1129 1156 2573 Fragments arranged by size: 2573 1156 1129 1059 814 239 BmgBI CAC'GTC Cuts at: 1318 3414 1318 Size: 2096 4874 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3217 3336 3712 Size: 416 53 83 399 24 1800 119 376 Cuts at: 3712 3814 4701 5659 442 Size: 102 887 958 1753 Fragments arranged by size: 1800 1753 958 887 416 399 376 119 102 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6970 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3250 3979 3250 Size: 729 6241 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2573 3199 5630 1239 Size: 1334 626 2431 2579 Fragments arranged by size: 2579 2431 1334 626 Bpu10I CC'TnA_GC Cuts at: 3148 3148 Size: 6970 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4211 5095 5960 6258 4211 Size: 884 865 298 4923 Fragments arranged by size: 4923 884 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4131 4244 5633 882 Size: 617 2632 113 1389 2219 Fragments arranged by size: 2632 2219 1389 617 113 BsaAI yAC'GTr Cuts at: 493 1204 4234 4267 4761 493 Size: 711 3030 33 494 2702 Fragments arranged by size: 3030 2702 711 494 33 BsaBI GATnn'nnATC Cuts at: 4151 6964 4151 Size: 2813 4157 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4257 4777 5159 5794 Size: 53 83 186 936 2724 520 382 635 Cuts at: 5794 275 Size: 1451 Fragments arranged by size: 2724 1451 936 635 520 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5403 5801 6075 6222 6633 5403 Size: 398 274 147 411 5740 Fragments arranged by size: 5740 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2421 2451 3068 3098 3197 Size: 30 165 30 1018 30 617 30 99 Cuts at: 3197 3227 1178 Size: 30 4921 Fragments arranged by size: 4921 1018 617 165 99 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2480 2603 2606 1186 Size: 1294 123 3 5550 Fragments arranged by size: 5550 1294 123 3 BseYI C'CCAG_C Cuts at: 6173 6669 6173 Size: 496 6474 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2879 2897 3510 3941 1163 Size: 573 1143 18 613 431 4192 Fragments arranged by size: 4192 1143 613 573 431 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4420 5181 6209 1540 Size: 275 186 2419 761 1028 2301 Fragments arranged by size: 2419 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2843 3095 3480 3804 3960 4191 5031 5116 6187 Size: 252 385 324 156 231 840 85 1071 Cuts at: 6187 2843 Size: 3626 Fragments arranged by size: 3626 1071 840 385 324 252 231 156 85 BsmI GAATG_Cn' Cuts at: 4481 4574 4481 Size: 93 6877 BsmBI CGTCTCn'nnnn_ Cuts at: 2393 2393 Size: 6970 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2604 2843 3095 3480 3804 3902 3960 Size: 399 814 239 252 385 324 98 58 Cuts at: 3960 4191 4289 5031 5116 6187 1391 Size: 231 98 742 85 1071 2174 Fragments arranged by size: 2174 1071 814 742 399 385 324 252 239 231 98 98 85 58 BspEI T'CCGG_A Cuts at: 6633 6633 Size: 6970 BspHI T'CATG_A Cuts at: 3480 3636 4859 6589 3480 Size: 156 1223 1730 3861 Fragments arranged by size: 3861 1730 1223 156 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3412 3632 3873 4325 844 Size: 2568 220 241 452 3489 Fragments arranged by size: 3489 2568 452 241 220 BsrBI CCG'CTC Cuts at: 1394 1823 3084 4857 1394 Size: 429 1261 1773 3507 Fragments arranged by size: 3507 1773 1261 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3208 3740 5465 5639 65 Size: 1183 1960 532 1725 174 1396 Fragments arranged by size: 1960 1725 1396 1183 532 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2919 5614 5801 1700 Size: 110 1109 2695 187 2869 Fragments arranged by size: 2869 2695 1109 187 110 BsrGI T'GTAC_A Cuts at: 96 3576 96 Size: 3480 3490 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6970 BssSI C'ACGA_G Cuts at: 5030 6042 5030 Size: 1012 5958 BstAPI GCAn_nnn'nTGC Cuts at: 794 2159 2893 3030 3868 794 Size: 1365 734 137 838 3896 Fragments arranged by size: 3896 1365 838 734 137 BstBI TT'CG_AA Cuts at: 2007 5774 2007 Size: 3767 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6970 BstXI CCAn_nnnn'nTGG Cuts at: 3400 4148 3400 Size: 748 6222 BstYI r'GATC_y Cuts at: 1972 2184 2522 2644 4152 4300 4667 5052 5069 Size: 212 338 122 1508 148 367 385 17 Cuts at: 5069 6521 6607 6619 6965 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1508 1452 385 367 346 338 212 148 122 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2497 3358 513 Size: 552 957 475 861 4125 Fragments arranged by size: 4125 957 861 552 475 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6231 Fragments arranged by size: 6231 683 56 BtsI GCAGTG_nn' Cuts at: 3529 3759 4486 5280 5300 3529 Size: 230 727 794 20 5199 Fragments arranged by size: 5199 794 727 230 20 ClaI AT'CG_AT Cuts at: 1554 2853 1554 Size: 1299 5671 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6935 DraI TTT'AAA Cuts at: 2932 4274 2932 Size: 1342 5628 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5977 809 Size: 507 4661 1802 Fragments arranged by size: 4661 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3888 4417 5306 Size: 54 1425 50 275 18 2058 529 889 Cuts at: 5306 6935 8 Size: 1629 43 Fragments arranged by size: 2058 1629 1425 889 529 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4417 1537 Size: 275 2605 4090 Fragments arranged by size: 4090 2605 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2502 3568 4028 4179 4196 4900 1353 Size: 1149 1066 460 151 17 704 3423 Fragments arranged by size: 3423 1149 1066 704 460 151 17 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5557 5931 6077 387 Size: 1064 138 3968 374 146 1280 Fragments arranged by size: 3968 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3478 3958 4189 3478 Size: 480 231 6259 Fragments arranged by size: 6259 480 231 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2573 2716 3199 3207 3354 3479 Size: 393 470 471 143 483 8 147 125 Cuts at: 3479 3569 5033 5630 6417 6685 1239 Size: 90 1464 597 787 268 1524 Fragments arranged by size: 1524 1464 787 597 483 471 470 393 268 147 143 125 90 8 EcoO109I rG'GnC_Cy Cuts at: 3792 3792 Size: 6970 EcoRI G'AATT_C Cuts at: 3932 4279 4657 3932 Size: 347 378 6245 Fragments arranged by size: 6245 378 347 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3359 3442 3467 3359 Size: 83 25 6862 Fragments arranged by size: 6862 83 25 FspI TGC'GCA Cuts at: 5476 5476 Size: 6970 HaeII r_GCGC'y Cuts at: 1678 2926 6117 1678 Size: 1248 3191 2531 Fragments arranged by size: 3191 2531 1248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3197 3229 5611 5643 5685 Size: 32 135 32 1625 32 2382 32 42 Cuts at: 5685 5717 1373 Size: 32 2626 Fragments arranged by size: 2626 2382 1625 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4314 4560 669 Size: 988 281 2376 246 3079 Fragments arranged by size: 3079 2376 988 281 246 HindIII A'AGCT_T Cuts at: 748 2207 748 Size: 1459 5511 HpaI GTT'AAC Cuts at: 4560 4560 Size: 6970 KpnI G_GTAC'C Cuts at: 4295 4295 Size: 6970 MfeI C'AATT_G Cuts at: 2739 4569 2739 Size: 1830 5140 MluI A'CGCG_T Cuts at: 6846 6846 Size: 6970 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2242 2398 6084 6268 1305 Size: 937 156 3686 184 2007 Fragments arranged by size: 3686 2007 937 184 156 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5148 Fragments arranged by size: 5148 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3572 4928 5287 5446 518 Size: 648 2406 1356 359 159 2042 Fragments arranged by size: 2406 2042 1356 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2881 3547 4386 5063 6211 6456 1606 Size: 1275 666 839 677 1148 245 2120 Fragments arranged by size: 2120 1275 1148 839 677 666 245 NaeI GCC'GGC Cuts at: 2921 2921 Size: 6970 NcoI C'CATG_G Cuts at: 513 1065 2022 2497 3358 513 Size: 552 957 475 861 4125 Fragments arranged by size: 4125 957 861 552 475 NdeI CA'TA_TG Cuts at: 387 2310 387 Size: 1923 5047 NgoMIV G'CCGG_C Cuts at: 2919 2919 Size: 6970 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6970 NotI GC'GGCC_GC Cuts at: 4417 4417 Size: 6970 NspI r_CATG'y Cuts at: 1132 1229 2095 2678 4012 4328 5873 1132 Size: 97 866 583 1334 316 1545 2229 Fragments arranged by size: 2229 1545 1334 866 583 316 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6970 PciI A'CATG_T Cuts at: 4008 5869 4008 Size: 1861 5109 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6970 PmeI GTTT'AAAC Cuts at: 4274 4274 Size: 6970 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5039 5071 6552 6584 5039 Size: 32 1481 32 5425 Fragments arranged by size: 5425 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3777 4256 1613 Size: 2164 479 4327 Fragments arranged by size: 4327 2164 479 PsiI TTA'TAA Cuts at: 4540 4540 Size: 6970 PstI C_TGCA'G Cuts at: 830 3536 3839 4322 830 Size: 2706 303 483 3478 Fragments arranged by size: 3478 2706 483 303 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6970 PvuII CAG'CTG Cuts at: 3547 3547 Size: 6970 SacI G_AGCT'C Cuts at: 3480 3960 4191 3480 Size: 480 231 6259 Fragments arranged by size: 6259 480 231 SalI G'TCGA_C Cuts at: 1936 4312 1936 Size: 2376 4594 SapI GCTCTTCn'nnn_ Cuts at: 4179 4196 4179 Size: 17 6953 SbfI CC_TGCA'GG Cuts at: 4322 4322 Size: 6970 ScaI AGT'ACT Cuts at: 2579 5218 6930 2579 Size: 2639 1712 2619 Fragments arranged by size: 2639 2619 1712 SfcI C'TryA_G Cuts at: 826 1045 3532 3835 4318 5453 6134 6325 826 Size: 219 2487 303 483 1135 681 191 1471 Fragments arranged by size: 2487 1471 1135 681 483 303 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6970 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6970 SmaI CCC'GGG Cuts at: 1872 2292 4297 1872 Size: 420 2005 4545 Fragments arranged by size: 4545 2005 420 SmlI C'TyrA_G Cuts at: 820 1017 1943 3854 4226 5074 5975 6237 820 Size: 197 926 1911 372 848 901 262 1553 Fragments arranged by size: 1911 1553 926 901 848 372 262 197 SnaBI TAC'GTA Cuts at: 493 4267 4761 493 Size: 3774 494 2702 Fragments arranged by size: 3774 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6970 SphI G_CATG'C Cuts at: 1132 4328 1132 Size: 3196 3774 SspI AAT'ATT Cuts at: 5 52 2416 4894 5 Size: 47 2364 2478 2081 Fragments arranged by size: 2478 2364 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6970 StyI C'CwwG_G Cuts at: 513 1065 2022 2497 3358 4409 513 Size: 552 957 475 861 1051 3074 Fragments arranged by size: 3074 1051 957 861 552 475 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2120 2860 3615 4268 4997 5014 5167 Size: 1255 540 740 755 653 729 17 153 Cuts at: 5167 5352 5797 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 755 740 729 653 540 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2577 3576 5216 Size: 274 80 33 51 1443 600 999 1640 Cuts at: 5216 6928 96 Size: 1712 138 Fragments arranged by size: 1712 1640 1443 999 600 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2421 2453 6930 6962 2421 Size: 32 4477 32 2429 Fragments arranged by size: 4477 2429 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6970 XbaI T'CTAG_A Cuts at: 4306 4306 Size: 6970 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4123 4123 Size: 6970 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6970 XmaI C'CCGG_G Cuts at: 1870 2290 4295 1870 Size: 420 2005 4545 Fragments arranged by size: 4545 2005 420 XmnI GAAnn'nnTTC Cuts at: 2711 3692 3932 5099 2711 Size: 981 240 1167 4582 Fragments arranged by size: 4582 1167 981 240 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4258 4778 276 Size: 53 83 186 936 2724 520 2468 Fragments arranged by size: 2724 2468 936 520 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI