Restriction map for
pFN21ASDA0388
(Linear) (Six-base) MAPSORT of: KIAA0388.seq Check: 8578 from: 1 to: 2241
KIAA0388.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1609 1850 2241
Size: 1609 241 391
Fragments arranged by size:
1609 391 241
AatII G_ACGT'C
Cuts at: 0 2237 2241
Size: 2237 4
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 693 1184 1331 1456 1546 2241
Size: 79 614 491 147 125 90 695
Fragments arranged by size:
695 614 491 147 125 90 79
AflII C'TTAA_G
Cuts at: 0 1831 2241
Size: 1831 410
AflIII A'CryG_T
Cuts at: 0 1985 2241
Size: 1985 256
AleI CACnn'nnGTG
Cuts at: 0 1549 2241
Size: 1549 692
AlwNI CAG_nnn'CTG
Cuts at: 0 1132 1601 2241
Size: 1132 469 640
Fragments arranged by size:
1132 640 469
ApaLI G'TGCA_C
Cuts at: 0 816 2241
Size: 816 1425
ApoI r'AATT_y
Cuts at: 0 132 1623 1909 2051 2241
Size: 132 1491 286 142 190
Fragments arranged by size:
1491 286 190 142 132
AvaI C'yCGr_G
Cuts at: 0 267 2241
Size: 267 1974
BamHI G'GATC_C
Cuts at: 0 499 2241
Size: 499 1742
BanI G'GyrC_C
Cuts at: 0 1091 1339 1876 2241
Size: 1091 248 537 365
Fragments arranged by size:
1091 537 365 248
BanII G_rGCy'C
Cuts at: 0 1457 1937 2168 2241
Size: 1457 480 231 73
Fragments arranged by size:
1457 480 231 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 1323 2241
Size: 1323 918
BbvCI CC'TCA_GC
Cuts at: 0 1125 2241
Size: 1125 1116
BclI T'GATC_A
Cuts at: 0 449 2241
Size: 449 1792
BglI GCCn_nnn'nGGC
Cuts at: 0 2143 2241
Size: 2143 98
BglII A'GATC_T
Cuts at: 0 621 2241
Size: 621 1620
Bme1580I G_kGCm'C
Cuts at: 0 581 820 1879 2241
Size: 581 239 1059 362
Fragments arranged by size:
1059 581 362 239
BmgBI CAC'GTC
Cuts at: 0 1391 2241
Size: 1391 850
BmrI ACTGGGnnnn_n'
Cuts at: 0 1194 1313 1689 1791 2241
Size: 1194 119 376 102 450
Fragments arranged by size:
1194 450 376 119 102
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1227 1956 2241
Size: 1227 729 285
Fragments arranged by size:
1227 729 285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 1176 2241
Size: 550 626 1065
Fragments arranged by size:
1065 626 550
Bpu10I CC'TnA_GC
Cuts at: 0 1125 2241
Size: 1125 1116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2188 2241
Size: 2188 53
BsaI GGTCTCn'nnnn_
Cuts at: 0 2108 2221 2241
Size: 2108 113 20
Fragments arranged by size:
2108 113 20
BsaAI yAC'GTr
Cuts at: 0 2211 2241
Size: 2211 30
BsaBI GATnn'nnATC
Cuts at: 0 2128 2241
Size: 2128 113
BsaHI Gr'CG_yC
Cuts at: 0 2234 2241
Size: 2234 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 398 428 1045 1075 1174 1204 2241
Size: 398 30 617 30 99 30 1037
Fragments arranged by size:
1037 617 398 99 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 457 580 583 2241
Size: 457 123 3 1658
Fragments arranged by size:
1658 457 123 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 874 1487 1918 2241
Size: 856 18 613 431 323
Fragments arranged by size:
856 613 431 323 18
BsiHKAI G_wGCw'C
Cuts at: 0 820 1072 1457 1781 1937 2168 2241
Size: 820 252 385 324 156 231 73
Fragments arranged by size:
820 385 324 252 231 156 73
BsmBI CGTCTCn'nnnn_
Cuts at: 0 370 2241
Size: 370 1871
Bsp1286I G_dGCh'C
Cuts at: 0 581 820 1072 1457 1781 1879 1937 2168
Size: 581 239 252 385 324 98 58 231
Cuts at: 2168 2241
Size: 73
Fragments arranged by size:
581 385 324 252 239 231 98 73
58
BspHI T'CATG_A
Cuts at: 0 1457 1613 2241
Size: 1457 156 628
Fragments arranged by size:
1457 628 156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1389 1609 1850 2241
Size: 1389 220 241 391
Fragments arranged by size:
1389 391 241 220
BsrBI CCG'CTC
Cuts at: 0 1061 2241
Size: 1061 1180
BsrDI GCAATG_nn'
Cuts at: 0 1185 1717 2241
Size: 1185 532 524
Fragments arranged by size:
1185 532 524
BsrFI r'CCGG_y
Cuts at: 0 896 2241
Size: 896 1345
BsrGI T'GTAC_A
Cuts at: 0 1553 2241
Size: 1553 688
BstAPI GCAn_nnn'nTGC
Cuts at: 0 136 870 1007 1845 2241
Size: 136 734 137 838 396
Fragments arranged by size:
838 734 396 137 136
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1377 2125 2241
Size: 1377 748 116
Fragments arranged by size:
1377 748 116
BstYI r'GATC_y
Cuts at: 0 161 499 621 2129 2241
Size: 161 338 122 1508 112
Fragments arranged by size:
1508 338 161 122 112
BtgI C'CryG_G
Cuts at: 0 474 1335 2241
Size: 474 861 906
Fragments arranged by size:
906 861 474
BtsI GCAGTG_nn'
Cuts at: 0 1506 1736 2241
Size: 1506 230 505
Fragments arranged by size:
1506 505 230
ClaI AT'CG_AT
Cuts at: 0 830 2241
Size: 830 1411
DraI TTT'AAA
Cuts at: 0 909 2241
Size: 909 1332
EaeI y'GGCC_r
Cuts at: 0 1865 2241
Size: 1865 376
EarI CTCTTCn'nnn_
Cuts at: 0 479 1545 2005 2156 2173 2241
Size: 479 1066 460 151 17 68
Fragments arranged by size:
1066 479 460 151 68 17
EcoICRI GAG'CTC
Cuts at: 0 1455 1935 2166 2241
Size: 1455 480 231 75
Fragments arranged by size:
1455 480 231 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 550 693 1176 1184 1331 1456 1546
Size: 79 471 143 483 8 147 125 90
Cuts at: 1546 2241
Size: 695
Fragments arranged by size:
695 483 471 147 143 125 90 79
8
EcoO109I rG'GnC_Cy
Cuts at: 0 1769 2241
Size: 1769 472
EcoRI G'AATT_C
Cuts at: 0 1909 2241
Size: 1909 332
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1336 1419 1444 2241
Size: 1336 83 25 797
Fragments arranged by size:
1336 797 83 25
HaeII r_GCGC'y
Cuts at: 0 903 2241
Size: 903 1338
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1174 1206 2241
Size: 1174 32 1035
Fragments arranged by size:
1174 1035 32
HindIII A'AGCT_T
Cuts at: 0 184 2241
Size: 184 2057
MfeI C'AATT_G
Cuts at: 0 716 2241
Size: 716 1525
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 375 2241
Size: 219 156 1866
Fragments arranged by size:
1866 219 156
MslI CAynn'nnrTG
Cuts at: 0 1549 2241
Size: 1549 692
MspA1I CmG'CkG
Cuts at: 0 858 1524 2241
Size: 858 666 717
Fragments arranged by size:
858 717 666
NaeI GCC'GGC
Cuts at: 0 898 2241
Size: 898 1343
NcoI C'CATG_G
Cuts at: 0 474 1335 2241
Size: 474 861 906
Fragments arranged by size:
906 861 474
NdeI CA'TA_TG
Cuts at: 0 287 2241
Size: 287 1954
NgoMIV G'CCGG_C
Cuts at: 0 896 2241
Size: 896 1345
NspI r_CATG'y
Cuts at: 0 72 655 1989 2241
Size: 72 583 1334 252
Fragments arranged by size:
1334 583 252 72
PciI A'CATG_T
Cuts at: 0 1985 2241
Size: 1985 256
PshAI GACnn'nnGTC
Cuts at: 0 1754 2233 2241
Size: 1754 479 8
Fragments arranged by size:
1754 479 8
PstI C_TGCA'G
Cuts at: 0 1513 1816 2241
Size: 1513 303 425
Fragments arranged by size:
1513 425 303
PvuII CAG'CTG
Cuts at: 0 1524 2241
Size: 1524 717
SacI G_AGCT'C
Cuts at: 0 1457 1937 2168 2241
Size: 1457 480 231 73
Fragments arranged by size:
1457 480 231 73
SapI GCTCTTCn'nnn_
Cuts at: 0 2156 2173 2241
Size: 2156 17 68
Fragments arranged by size:
2156 68 17
ScaI AGT'ACT
Cuts at: 0 556 2241
Size: 556 1685
SfcI C'TryA_G
Cuts at: 0 1509 1812 2241
Size: 1509 303 429
Fragments arranged by size:
1509 429 303
SmaI CCC'GGG
Cuts at: 0 269 2241
Size: 269 1972
SmlI C'TyrA_G
Cuts at: 0 1831 2203 2241
Size: 1831 372 38
Fragments arranged by size:
1831 372 38
SspI AAT'ATT
Cuts at: 0 393 2241
Size: 393 1848
StyI C'CwwG_G
Cuts at: 0 474 1335 2241
Size: 474 861 906
Fragments arranged by size:
906 861 474
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 97 837 1592 2241
Size: 97 740 755 649
Fragments arranged by size:
755 740 649 97
TatI w'GTAC_w
Cuts at: 0 554 1553 2241
Size: 554 999 688
Fragments arranged by size:
999 688 554
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 398 430 2241
Size: 398 32 1811
Fragments arranged by size:
1811 398 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2100 2241
Size: 2100 141
XmaI C'CCGG_G
Cuts at: 0 267 2241
Size: 267 1974
XmnI GAAnn'nnTTC
Cuts at: 0 688 1669 1909 2241
Size: 688 981 240 332
Fragments arranged by size:
981 688 332 240
ZraI GAC'GTC
Cuts at: 0 2235 2241
Size: 2235 6
Enzymes that do cut:
AarI AatII AcuI AflII AflIII AleI AlwNI ApaLI
ApoI AvaI BamHI BanI BanII BbsI BbvCI BclI
BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BsgI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstXI BstYI BtgI BtsI ClaI DraI
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII
Hin4I HindIII MfeI MmeI MslI MspA1I NaeI NcoI
NdeI NgoMIV NspI PciI PshAI PstI PvuII SacI
SapI ScaI SfcI SmaI SmlI SspI StyI TaqII
TatI TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AgeI AhdI AlfI AloI
ApaI AscI AseI AsiSI AvrII BaeI BbeI BcgI
BciVI BfrBI BlpI BmtI BsaWI BseYI BsiEI BsiWI
BsmI BspEI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI CspCI DraIII DrdI EagI EciI EcoNI EcoRV
FseI FspI FspAI HincII HpaI KasI KpnI MluI
MscI NarI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI StuI SwaI Tth111I XbaI XhoI