Restriction map for
pFN21ASDA0385
##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AclI AflII AgeI Aor51HI
AscI AspI AviII AvrII BbeI
BcgI BclI BfrI BlnI BsiEI
BsiWI BspDI BspHI BsrBI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
FbaI FseI FspI HpaI KasI
KspI MfeI MluI MunI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmeI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 369 GGTACC G^GTACC
AccIII 3932 TCCGGA T^CCGGA
AcyI 2527 GGCGTC G[GA]^CG[CT]C
AhdI 1383 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 932 GTGCAC G^TGCAC
ApaLI 932 GTGCAC G^TGCAC
AseI 3019 ATTAAT AT^TAAT
AsnI 3019 ATTAAT AT^TAAT
Asp718I 369 GGTACC G^GTACC
AspEI 1383 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1203 GGATCC G^GATCC
BbrPI 1478 CACGTG CAC^GTG
BciVI 2280 GGATAC GTATCC(6/5)
BglI 840 GCCATTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1076 AGATCT A^GATCT
BsaAI 1478 CACGTG [CT]AC^GT[GA]
BsaBI 2673 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2527 GGCGTC G[GA]^CG[CT]C
BseAI 3932 TCCGGA T^CCGGA
BsmBI 2529 CGTCTC CGTCTC(1/5)
Bsp1407I 1988 TGTACA T^GTACA
BspEI 3932 TCCGGA T^CCGGA
BspLU11I 1536 ACATGT A^CATGT
BsrFI 1286 ACCGGC [GA]^CCGG[CT]
BsrGI 1988 TGTACA T^GTACA
Cfr10I 1286 ACCGGC [GA]^CCGG[CT]
Eam1105I 1383 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3901 GAATTC G^AATTC
EcoT22I 2635 ATGCAT ATGCA^T
Hin1I 2527 GGCGTC G[GA]^CG[CT]C
HincII 1684 GTTGAC GT[CT]^[GA]AC
HindII 1684 GTTGAC GT[CT]^[GA]AC
HindIII 1959 AAGCTT A^AGCTT
KpnI 369 GGTACC GGTAC^C
MaeII 1479 ACGT A^CGT
MamI 2673 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3932 TCCGGA T^CCGGA
MseI 3020 TTAA T^TAA
NsiI 2635 ATGCAT ATGCA^T
PmaCI 1478 CACGTG CAC^GTG
PmlI 1478 CACGTG CAC^GTG
PshBI 3019 ATTAAT AT^TAAT
SapI 996 GCTCTTC GCTCTTC(1/4)
ScaI 3431 AGTACT AGT^ACT
SmaI 3146 CCCGGG CCC^GGG
SphI 4037 GCATGC GCATG^C
SspBI 1988 TGTACA T^GTACA
StuI 2840 AGGCCT AGG^CCT
Tru9I 3020 TTAA T^TAA
XmaCI 3146 CCCGGG C^CCGGG
XmaI 3146 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
ApaI 134 GGGCCC GGGCC^C
2293 GGGCCC
Asp700I 1956 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3459 GAACATTTTC
BalI 2936 TGGCCA TGG^CCA
2954 TGGCCA
BlpI 3317 GCTCAGC GC^T[ACGT]AGC
3325 GCTGAGC
Bpu1102I 3317 GCTCAGC GC^T[ACGT]AGC
3325 GCTGAGC
BsaWI 3449 ACCGGA [AT]^CCGG[AT]
3932 TCCGGA
BsmI 776 GCATTC GAATGC(1/-1)
1757 GCATTC
BsrDI 937 CATTGC GCAATG(2/0)
1268 GCAATG
BssSI 1258 CACGAG CACGAG(-5/-1)
1414 CACGAG
CelII 3317 GCTCAGC GC^T[ACGT]AGC
3325 GCTGAGC
DrdI 205 GACCCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1687 GACCGCACAGTC
EaeI 2936 TGGCCA [CT]^GGCC[GA]
2954 TGGCCA
HgaI 1213 GACGC GACGC(5/10)
2528 GCGTC
MluNI 2936 TGGCCA TGG^CCA
2954 TGGCCA
MscI 2936 TGGCCA TGG^CCA
2954 TGGCCA
NdeI 1230 CATATG CA^TATG
3194 CATATG
PshAI 286 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3802 GACACGGGTC
SacI 1402 GAGCTC GAGCT^C
3328 GAGCTC
XcmI 121 CCAACTCGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
227 CCACTGAGTTGCTGG
XmnI 1956 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3459 GAACATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PaeR7I
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoI XhoII