(Circular) (Six-base) MAPSORT of: pFN21ASDA0385.seq Check: 3139 from: 1 to: 8839 pFN21ASDA0385 8839 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6649 278 Size: 53 83 186 936 5113 2468 Fragments arranged by size: 5113 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2100 3720 5055 5099 5628 6182 1937 Size: 163 1620 1335 44 529 554 4594 Fragments arranged by size: 4594 1620 1335 554 529 163 44 Acc65I G'GTAC_C Cuts at: 2392 6160 2392 Size: 3768 5071 AclI AA'CG_TT Cuts at: 1563 6966 7339 1563 Size: 5403 373 3063 Fragments arranged by size: 5403 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2586 3799 4799 6902 8286 8554 1632 Size: 954 1213 1000 2103 1384 268 1917 Fragments arranged by size: 2103 1917 1384 1213 1000 954 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8642 AflIII A'CryG_T Cuts at: 1203 3500 3502 3559 7738 8715 1203 Size: 2297 2 57 4179 977 1327 Fragments arranged by size: 4179 2297 1327 977 57 2 AgeI A'CCGG_T Cuts at: 7670 7670 Size: 8839 AhdI GACnn_n'nnGTC Cuts at: 3411 7568 3411 Size: 4157 4682 AleI CACnn'nnGTG Cuts at: 1166 3503 4227 5726 5774 1166 Size: 2337 724 1499 48 4231 Fragments arranged by size: 4231 2337 1499 724 48 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3026 4169 1184 Size: 1842 1143 5854 Fragments arranged by size: 5854 1842 1143 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3839 3871 3839 Size: 32 8807 AlwNI CAG_nnn'CTG Cuts at: 2411 3666 3744 4116 4847 5348 6582 8154 2411 Size: 1255 78 372 731 501 1234 1572 3096 Fragments arranged by size: 3096 1572 1255 1234 731 501 372 78 ApaI G_GGCC'C Cuts at: 2161 4320 2161 Size: 2159 6680 ApaLI G'TGCA_C Cuts at: 2955 6896 8052 2955 Size: 3941 1156 3742 Fragments arranged by size: 3941 3742 1156 ApoI r'AATT_y Cuts at: 1493 2108 2419 3338 4423 5924 6148 6374 6526 Size: 615 311 919 1085 1501 224 226 152 Cuts at: 6526 8635 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1501 1085 919 615 311 226 224 152 AseI AT'TA_AT Cuts at: 160 1032 5043 7393 160 Size: 872 4011 2350 1606 Fragments arranged by size: 4011 2350 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8839 AvaI C'yCGr_G Cuts at: 1870 1943 2148 2182 3064 4319 4944 5169 5583 Size: 73 205 34 882 1255 625 225 414 Cuts at: 5583 5760 6164 1870 Size: 177 404 4545 Fragments arranged by size: 4545 1255 882 625 414 404 225 205 177 73 34 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3491 3524 3491 Size: 33 8806 BamHI G'GATC_C Cuts at: 3226 6169 6536 3226 Size: 2943 367 5529 Fragments arranged by size: 5529 2943 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2392 2939 4179 4684 4708 Size: 325 217 627 605 547 1240 505 24 Cuts at: 4708 5765 6160 7615 618 Size: 1057 395 1455 1842 Fragments arranged by size: 1842 1455 1240 1057 627 605 547 505 395 325 217 24 BanII G_rGCy'C Cuts at: 1391 2161 2191 2537 2582 2660 3429 3639 4320 Size: 770 30 346 45 78 769 210 681 Cuts at: 4320 5208 5355 6158 1391 Size: 888 147 803 4072 Fragments arranged by size: 4072 888 803 770 769 681 346 210 147 78 45 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3269 4256 4451 4926 6129 928 Size: 971 1370 987 195 475 1203 3638 Fragments arranged by size: 3638 1370 1203 987 971 475 195 BbvCI CC'TCA_GC Cuts at: 3015 4848 5697 3015 Size: 1833 849 6157 Fragments arranged by size: 6157 1833 849 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7030 7064 1903 Size: 34 5093 34 3678 Fragments arranged by size: 5093 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4297 6731 7941 4297 Size: 2434 1210 5195 Fragments arranged by size: 5195 2434 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8839 BfrBI ATG'CAT Cuts at: 4660 4660 Size: 8839 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2869 7450 136 Size: 107 122 71 631 605 1197 4581 1525 Fragments arranged by size: 4581 1525 1197 631 605 122 107 71 BglII A'GATC_T Cuts at: 3099 8834 3099 Size: 5735 3104 BlpI GC'TnA_GC Cuts at: 5341 5349 6256 5341 Size: 8 907 7924 Fragments arranged by size: 7924 907 8 Bme1580I G_kGCm'C Cuts at: 1790 2161 2942 2959 4320 4638 4719 5768 6900 Size: 371 781 17 1361 318 81 1049 1132 Cuts at: 6900 8056 1790 Size: 1156 2573 Fragments arranged by size: 2573 1361 1156 1132 1049 781 371 318 81 17 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8839 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2025 2037 3211 Size: 416 53 83 399 24 608 12 1174 Cuts at: 3211 3910 4625 5043 5365 5630 5795 6113 6570 Size: 699 715 418 322 265 165 318 457 Cuts at: 6570 7528 442 Size: 958 1753 Fragments arranged by size: 1753 1174 958 715 699 608 457 418 416 399 322 318 265 165 83 53 24 12 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8839 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2527 2527 Size: 8839 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2106 2253 2442 3162 3288 4232 7499 1239 Size: 867 147 189 720 126 944 3267 2579 Fragments arranged by size: 3267 2579 944 867 720 189 147 126 Bpu10I CC'TnA_GC Cuts at: 3015 3423 3788 4848 5697 3015 Size: 408 365 1060 849 6157 Fragments arranged by size: 6157 1060 849 408 365 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2887 6964 7829 8127 2887 Size: 4077 865 298 3599 Fragments arranged by size: 4077 3599 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2082 2498 4208 4757 7502 882 Size: 617 583 416 1710 549 2745 2219 Fragments arranged by size: 2745 2219 1710 617 583 549 416 BsaAI yAC'GTr Cuts at: 493 1204 3503 6136 6630 493 Size: 711 2299 2633 494 2702 Fragments arranged by size: 2702 2633 2299 711 494 BsaBI GATnn'nnATC Cuts at: 4700 8833 4700 Size: 4133 4706 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4551 6646 7028 7663 Size: 53 83 186 936 3018 2095 382 635 Cuts at: 7663 275 Size: 1451 Fragments arranged by size: 3018 2095 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5472 5955 7272 7670 7944 8091 8502 5472 Size: 483 1317 398 274 147 411 5809 Fragments arranged by size: 5809 1317 483 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3631 3661 4559 4589 1178 Size: 30 165 30 2228 30 898 30 5428 Fragments arranged by size: 5428 2228 898 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2513 2516 2564 2567 2874 3419 4787 4865 Size: 1327 3 48 3 307 545 1368 78 Cuts at: 4865 5549 5606 5609 5636 1186 Size: 684 57 3 27 4389 Fragments arranged by size: 4389 1368 1327 684 545 307 78 57 48 27 3 3 3 BseYI C'CCAG_C Cuts at: 2260 2266 2411 3195 3737 4036 4492 5241 8042 Size: 6 145 784 542 299 456 749 2801 Cuts at: 8042 8538 2260 Size: 496 2561 Fragments arranged by size: 2801 2561 784 749 542 496 456 299 145 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2655 3148 3263 4923 5267 1163 Size: 573 919 493 115 1660 344 4735 Fragments arranged by size: 4735 1660 919 573 493 344 115 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6289 7050 8078 1540 Size: 275 186 4288 761 1028 2301 Fragments arranged by size: 4288 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2959 3429 3927 4748 5355 5601 6900 6985 8056 Size: 470 498 821 607 246 1299 85 1071 Cuts at: 8056 2959 Size: 3742 Fragments arranged by size: 3742 1299 1071 821 607 498 470 246 85 BsmI GAATG_Cn' Cuts at: 2799 3780 6350 6443 2799 Size: 981 2570 93 5195 Fragments arranged by size: 5195 2570 981 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4558 4558 Size: 8839 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2161 2191 2537 2582 2660 2942 2959 Size: 399 371 30 346 45 78 282 17 Cuts at: 2959 3429 3639 3927 4320 4638 4719 4748 5208 Size: 470 210 288 393 318 81 29 460 Cuts at: 5208 5355 5601 5768 6158 6900 6985 8056 1391 Size: 147 246 167 390 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 470 460 399 393 390 371 346 318 288 282 246 210 167 147 85 81 78 45 30 29 17 BspEI T'CCGG_A Cuts at: 5955 8502 5955 Size: 2547 6292 BspHI T'CATG_A Cuts at: 6728 8458 6728 Size: 1730 7109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2205 3479 4085 4161 5988 6194 844 Size: 1361 1274 606 76 1827 206 3489 Fragments arranged by size: 3489 1827 1361 1274 606 206 76 BsrBI CCG'CTC Cuts at: 1394 1823 6726 1394 Size: 429 4903 3507 Fragments arranged by size: 4903 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2959 3298 7334 7508 65 Size: 1183 1711 339 4036 174 1396 Fragments arranged by size: 4036 1711 1396 1183 339 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3309 7483 7670 1700 Size: 110 1499 4174 187 2869 Fragments arranged by size: 4174 2869 1499 187 110 BsrGI T'GTAC_A Cuts at: 96 4011 96 Size: 3915 4924 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8839 BssSI C'ACGA_G Cuts at: 3281 3437 6899 7911 3281 Size: 156 3462 1012 4209 Fragments arranged by size: 4209 3462 1012 156 BstAPI GCAn_nnn'nTGC Cuts at: 794 2339 3255 3666 5458 794 Size: 1545 916 411 1792 4175 Fragments arranged by size: 4175 1792 1545 916 411 BstBI TT'CG_AA Cuts at: 2007 7643 2007 Size: 5636 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8839 BstXI CCAn_nnnn'nTGG Cuts at: 3266 3519 4229 4738 4804 4987 5776 3266 Size: 253 710 509 66 183 789 6329 Fragments arranged by size: 6329 789 710 509 253 183 66 BstYI r'GATC_y Cuts at: 1972 2119 3099 3226 4236 4434 4788 6169 6536 Size: 147 980 127 1010 198 354 1381 367 Cuts at: 6536 6921 6938 8390 8476 8488 8834 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1381 1010 980 385 367 354 346 198 147 127 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2319 2385 2433 2775 3771 3828 5088 2319 Size: 66 48 342 996 57 1260 6070 Fragments arranged by size: 6070 1260 996 342 66 57 48 BtgI C'CryG_G Cuts at: 513 1065 2022 2311 2829 4962 6036 513 Size: 552 957 289 518 2133 1074 3316 Fragments arranged by size: 3316 2133 1074 957 552 518 289 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5986 487 Size: 683 56 4760 3340 Fragments arranged by size: 4760 3340 683 56 BtsI GCAGTG_nn' Cuts at: 2474 2940 2972 4135 4265 4461 6355 7149 7169 Size: 466 32 1163 130 196 1894 794 20 Cuts at: 7169 2474 Size: 4144 Fragments arranged by size: 4144 1894 1163 794 466 196 130 32 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8839 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8804 DraI TTT'AAA Cuts at: 6143 6143 Size: 8839 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2234 3716 7846 809 Size: 507 918 1482 4130 1802 Fragments arranged by size: 4130 1802 1482 918 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4959 4977 6286 Size: 54 1425 50 275 18 3129 18 1309 Cuts at: 6286 7175 8804 8 Size: 889 1629 43 Fragments arranged by size: 3129 1629 1425 1309 889 275 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 6286 1537 Size: 275 4474 4090 Fragments arranged by size: 4474 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2490 2729 3026 3437 3818 4590 5840 6769 Size: 1137 239 297 411 381 772 1250 929 Cuts at: 6769 1353 Size: 3423 Fragments arranged by size: 3423 1250 1137 929 772 411 381 297 239 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5795 7426 7800 7946 387 Size: 1064 138 4206 1631 374 146 1280 Fragments arranged by size: 4206 1631 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3427 5353 3427 Size: 1926 6913 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2106 2253 2442 2586 3162 3288 3799 Size: 393 474 147 189 144 576 126 511 Cuts at: 3799 4232 4799 6902 7499 8286 8554 1239 Size: 433 567 2103 597 787 268 1524 Fragments arranged by size: 2103 1524 787 597 576 567 511 474 433 393 268 189 147 144 126 EcoNI CCTnn'n_nnAGG Cuts at: 2367 2403 3188 3281 2367 Size: 36 785 93 7925 Fragments arranged by size: 7925 785 93 36 EcoO109I rG'GnC_Cy Cuts at: 2158 3274 4404 5084 5831 2158 Size: 1116 1130 680 747 5166 Fragments arranged by size: 5166 1130 1116 747 680 EcoRI G'AATT_C Cuts at: 5924 6148 6526 5924 Size: 224 378 8237 Fragments arranged by size: 8237 378 224 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3601 4057 3601 Size: 456 8383 FspI TGC'GCA Cuts at: 7345 7345 Size: 8839 HaeII r_GCGC'y Cuts at: 1678 2881 2929 3208 4198 7986 1678 Size: 1203 48 279 990 3788 2531 Fragments arranged by size: 3788 2531 1203 990 279 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2980 3012 3397 3429 3539 Size: 32 135 32 1408 32 385 32 110 Cuts at: 3539 3571 4354 4386 4528 4559 4560 4591 4850 Size: 32 783 32 142 31 1 31 259 Cuts at: 4850 4882 4938 4970 5081 5113 5119 5151 7480 Size: 32 56 32 111 32 6 32 2329 Cuts at: 7480 7512 7554 7586 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2329 1408 783 385 259 142 135 111 110 56 42 32 32 32 32 32 32 32 32 32 32 32 32 31 31 6 1 HincII GTy'rAC Cuts at: 669 1657 1938 3709 6183 6429 669 Size: 988 281 1771 2474 246 3079 Fragments arranged by size: 3079 2474 1771 988 281 246 HindIII A'AGCT_T Cuts at: 748 3982 748 Size: 3234 5605 HpaI GTT'AAC Cuts at: 6429 6429 Size: 8839 KpnI G_GTAC'C Cuts at: 2396 6164 2396 Size: 3768 5071 MfeI C'AATT_G Cuts at: 6438 6438 Size: 8839 MluI A'CGCG_T Cuts at: 8715 8715 Size: 8839 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4821 6033 7953 8137 1305 Size: 3516 1212 1920 184 2007 Fragments arranged by size: 3516 2007 1920 1212 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4961 4979 10 Size: 54 1425 343 3129 18 3870 Fragments arranged by size: 3870 3129 1425 343 54 18 MslI CAynn'nnrTG Cuts at: 518 1166 2804 2945 3503 4227 4631 4643 5657 Size: 648 1638 141 558 724 404 12 1014 Cuts at: 5657 5726 5774 6797 7156 7315 518 Size: 69 48 1023 359 159 2042 Fragments arranged by size: 2042 1638 1023 1014 724 648 558 404 359 159 141 69 48 12 MspA1I CmG'CkG Cuts at: 1606 3741 3969 5345 5696 5904 6255 6932 8080 Size: 2135 228 1376 351 208 351 677 1148 Cuts at: 8080 8325 1606 Size: 245 2120 Fragments arranged by size: 2135 2120 1376 1148 677 351 351 245 228 208 NcoI C'CATG_G Cuts at: 513 1065 2022 2829 4962 6036 513 Size: 552 957 807 2133 1074 3316 Fragments arranged by size: 3316 2133 1074 957 807 552 NdeI CA'TA_TG Cuts at: 387 3254 5218 387 Size: 2867 1964 4008 Fragments arranged by size: 4008 2867 1964 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8839 NotI GC'GGCC_GC Cuts at: 6286 6286 Size: 8839 NsiI A_TGCA'T Cuts at: 4662 4662 Size: 8839 NspI r_CATG'y Cuts at: 1132 1229 2893 3563 5710 6052 6064 6197 7742 Size: 97 1664 670 2147 342 12 133 1545 Cuts at: 7742 1132 Size: 2229 Fragments arranged by size: 2229 2147 1664 1545 670 342 133 97 12 PasI CC'CwG_GG Cuts at: 1348 2540 1348 Size: 1192 7647 PciI A'CATG_T Cuts at: 3559 7738 3559 Size: 4179 4660 PflMI CCAn_nnn'nTGG Cuts at: 1108 2411 2693 3744 4224 1108 Size: 1303 282 1051 480 5723 Fragments arranged by size: 5723 1303 1051 480 282 PfoI T'CCnGG_A Cuts at: 2238 4914 5833 2238 Size: 2676 919 5244 Fragments arranged by size: 5244 2676 919 PmeI GTTT'AAAC Cuts at: 6143 6143 Size: 8839 PmlI CAC'GTG Cuts at: 3503 3503 Size: 8839 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6908 6940 8421 8453 6908 Size: 32 1481 32 7294 Fragments arranged by size: 7294 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3274 4404 5831 3274 Size: 1130 1427 6282 Fragments arranged by size: 6282 1427 1130 PshAI GACnn'nnGTC Cuts at: 1613 2313 5829 1613 Size: 700 3516 4623 Fragments arranged by size: 4623 3516 700 PsiI TTA'TAA Cuts at: 6409 6409 Size: 8839 PspOMI G'GGCC_C Cuts at: 2157 4316 2157 Size: 2159 6680 PspXI vC'TCGA_Gb Cuts at: 2148 2148 Size: 8839 PstI C_TGCA'G Cuts at: 830 2481 2935 2979 3251 3320 3662 3734 3992 Size: 1651 454 44 272 69 342 72 258 Cuts at: 3992 4130 5691 6191 830 Size: 138 1561 500 3478 Fragments arranged by size: 3478 1651 1561 500 454 342 272 258 138 72 69 44 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8839 PvuII CAG'CTG Cuts at: 3741 5345 5696 3741 Size: 1604 351 6884 Fragments arranged by size: 6884 1604 351 SacI G_AGCT'C Cuts at: 3429 5355 3429 Size: 1926 6913 SalI G'TCGA_C Cuts at: 1936 6181 1936 Size: 4245 4594 SapI GCTCTTCn'nnn_ Cuts at: 3026 3026 Size: 8839 SbfI CC_TGCA'GG Cuts at: 6191 6191 Size: 8839 ScaI AGT'ACT Cuts at: 5456 7087 8799 5456 Size: 1631 1712 5496 Fragments arranged by size: 5496 1712 1631 SfcI C'TryA_G Cuts at: 826 1045 2477 2931 2975 3247 3316 3658 3730 Size: 219 1432 454 44 272 69 342 72 Cuts at: 3730 3988 4126 4276 5687 6187 7322 8003 8194 Size: 258 138 150 1411 500 1135 681 191 Cuts at: 8194 826 Size: 1471 Fragments arranged by size: 1471 1432 1411 1135 681 500 454 342 272 258 219 191 150 138 72 69 44 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8839 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8839 SmaI CCC'GGG Cuts at: 1872 5171 6166 1872 Size: 3299 995 4545 Fragments arranged by size: 4545 3299 995 SmlI C'TyrA_G Cuts at: 820 1017 1943 2148 2902 4944 5583 6943 7844 Size: 197 926 205 754 2042 639 1360 901 Cuts at: 7844 8106 820 Size: 262 1553 Fragments arranged by size: 2042 1553 1360 926 901 754 639 262 205 197 SnaBI TAC'GTA Cuts at: 493 6136 6630 493 Size: 5643 494 2702 Fragments arranged by size: 5643 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8839 SphI G_CATG'C Cuts at: 1132 6064 6197 1132 Size: 4932 133 3774 Fragments arranged by size: 4932 3774 133 SspI AAT'ATT Cuts at: 5 52 6763 5 Size: 47 6711 2081 Fragments arranged by size: 6711 2081 47 StuI AGG'CCT Cuts at: 1517 4865 1517 Size: 3348 5491 StyI C'CwwG_G Cuts at: 513 1065 2022 2563 2829 3111 4737 4803 4962 Size: 552 957 541 266 282 1626 66 159 Cuts at: 4962 5772 6036 6278 513 Size: 810 264 242 3074 Fragments arranged by size: 3074 1626 957 810 552 541 282 266 264 242 159 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5233 6866 6883 7036 7221 7666 325 Size: 1255 3653 1633 17 153 185 445 1498 Fragments arranged by size: 3653 1633 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4011 4058 4665 Size: 274 80 33 51 1443 2034 47 607 Cuts at: 4665 5454 7085 8797 96 Size: 789 1631 1712 138 Fragments arranged by size: 2034 1712 1631 1443 789 607 274 138 80 51 47 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3631 3663 8799 8831 3631 Size: 32 5136 32 3639 Fragments arranged by size: 5136 3639 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8839 XbaI T'CTAG_A Cuts at: 6175 6175 Size: 8839 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2151 2257 2151 Size: 106 8733 XhoI C'TCGA_G Cuts at: 1943 2148 4944 5583 1943 Size: 205 2796 639 5199 Fragments arranged by size: 5199 2796 639 205 XmaI C'CCGG_G Cuts at: 1870 5169 6164 1870 Size: 3299 995 4545 Fragments arranged by size: 4545 3299 995 XmnI GAAnn'nnTTC Cuts at: 3983 5486 6968 3983 Size: 1503 1482 5854 Fragments arranged by size: 5854 1503 1482 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6647 276 Size: 53 83 186 936 5113 2468 Fragments arranged by size: 5113 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbeI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI