Restriction map for
pFN21ASDA0383
(Circular) (Six-base) MAPSORT of: pFN21ASDA0383.seq Check: 2602 from: 1 to: 10072
pFN21ASDA0383 10072 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4261 4261
Size: 10072
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7882 278
Size: 53 83 186 936 6346 2468
Fragments arranged by size:
6346 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5583 6135 7415 1937
Size: 3646 552 1280 4594
Fragments arranged by size:
4594 3646 1280 552
Acc65I G'GTAC_C
Cuts at: 3300 7393 3300
Size: 4093 5979
AclI AA'CG_TT
Cuts at: 1563 3593 8199 8572 1563
Size: 2030 4606 373 3063
Fragments arranged by size:
4606 3063 2030 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2127 2420 3234 4161 5016 5655 6574 8135
Size: 495 293 814 927 855 639 919 1561
Cuts at: 8135 9519 9787 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1561 1384 927 919 855 814 639
495 293 268
AflII C'TTAA_G
Cuts at: 820 1017 2806 820
Size: 197 1789 8086
Fragments arranged by size:
8086 1789 197
AflIII A'CryG_T
Cuts at: 1203 5132 8971 9948 1203
Size: 3929 3839 977 1327
Fragments arranged by size:
3929 3839 1327 977
AgeI A'CCGG_T
Cuts at: 8903 8903
Size: 10072
AhdI GACnn_n'nnGTC
Cuts at: 8801 8801
Size: 10072
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10072
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4269 6365 7026 1184
Size: 3085 2096 661 4230
Fragments arranged by size:
4230 3085 2096 661
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4203 4235 6242 6274 4203
Size: 32 2007 32 8001
Fragments arranged by size:
8001 2007 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2165 2476 2844 4139 4330 6228 6442 6953 7815
Size: 311 368 1295 191 1898 214 511 862
Cuts at: 7815 9387 2165
Size: 1572 2850
Fragments arranged by size:
2850 1898 1572 1295 862 511 368 311
214 191
ApaLI G'TGCA_C
Cuts at: 8129 9285 8129
Size: 1156 8916
ApoI r'AATT_y
Cuts at: 1493 2771 3317 3401 3486 3540 4960 7381 7607
Size: 1278 546 84 85 54 1420 2421 226
Cuts at: 7607 7759 9868 1493
Size: 152 2109 1697
Fragments arranged by size:
2421 2109 1697 1420 1278 546 226 152
85 84 54
AseI AT'TA_AT
Cuts at: 160 1032 8626 160
Size: 872 7594 1606
Fragments arranged by size:
7594 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10072
AvaI C'yCGr_G
Cuts at: 1870 1943 6500 7397 1870
Size: 73 4557 897 4545
Fragments arranged by size:
4557 4545 897 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2564 2597 2564
Size: 33 10039
BamHI G'GATC_C
Cuts at: 3104 7402 7769 3104
Size: 4298 367 5407
Fragments arranged by size:
5407 4298 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2572 3300 7232 7393 8848
Size: 325 217 627 785 728 3932 161 1455
Cuts at: 8848 618
Size: 1842
Fragments arranged by size:
3932 1842 1455 785 728 627 325 217
161
BanII G_rGCy'C
Cuts at: 1391 4623 5613 7391 1391
Size: 3232 990 1778 4072
Fragments arranged by size:
4072 3232 1778 990
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2162 3294 4949 5352 928
Size: 971 263 1132 1655 403 5648
Fragments arranged by size:
5648 1655 1132 971 403 263
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5695 5729 8263 8297 1903
Size: 34 3758 34 2534 34 3678
Fragments arranged by size:
3758 3678 2534 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2592 3662 7964 9174 2592
Size: 1070 4302 1210 3490
Fragments arranged by size:
4302 3490 1210 1070
BclI T'GATC_A
Cuts at: 1548 3723 5365 6631 1548
Size: 2175 1642 1266 4989
Fragments arranged by size:
4989 2175 1642 1266
BfrBI ATG'CAT
Cuts at: 3116 3116
Size: 10072
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8683 136
Size: 107 122 71 631 605 7011 1525
Fragments arranged by size:
7011 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 10067 10067
Size: 10072
BlpI GC'TnA_GC
Cuts at: 4170 7489 4170
Size: 3319 6753
Bme1580I G_kGCm'C
Cuts at: 1790 2575 3950 5140 7235 8133 9289 1790
Size: 785 1375 1190 2095 898 1156 2573
Fragments arranged by size:
2573 2095 1375 1190 1156 898 785
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10072
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2492 2580 5046
Size: 416 53 83 399 24 1075 88 2466
Cuts at: 5046 5097 5936 7803 8761 442
Size: 51 839 1867 958 1753
Fragments arranged by size:
2466 1867 1753 1075 958 839 416 399
88 83 53 51 24
BmtI G_CTAG'C
Cuts at: 1056 4194 1056
Size: 3138 6934
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3459 4352 4682 6262 8732 1239
Size: 2220 893 330 1580 2470 2579
Fragments arranged by size:
2579 2470 2220 1580 893 330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2396 5091 5826 6384 8197 9062 9360 2396
Size: 2695 735 558 1813 865 298 3108
Fragments arranged by size:
3108 2695 1813 865 735 558 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3816 4580 8735 882
Size: 617 2317 764 4155 2219
Fragments arranged by size:
4155 2317 2219 764 617
BsaAI yAC'GTr
Cuts at: 493 1204 7369 7863 493
Size: 711 6165 494 2702
Fragments arranged by size:
6165 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 10066 10066
Size: 10072
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5580 7879 8261 8896
Size: 53 83 186 936 4047 2299 382 635
Cuts at: 8896 275
Size: 1451
Fragments arranged by size:
4047 2299 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4946 8505 8903 9177 9324 9735 4946
Size: 3559 398 274 147 411 5283
Fragments arranged by size:
5283 3559 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5262 5292 6242 6260 6272
Size: 30 165 30 3859 30 950 18 12
Cuts at: 6272 6290 6790 6820 1178
Size: 18 500 30 4430
Fragments arranged by size:
4430 3859 950 500 165 30 30 30
30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2383 2393 2714 3340 4379 4382 4385 4394
Size: 1197 10 321 626 1039 3 3 9
Cuts at: 4394 4397 4409 4436 5084 5087 5225 5228 5231
Size: 3 12 27 648 3 138 3 3
Cuts at: 5231 5234 5283 5390 5393 5672 6387 6792 6795
Size: 3 49 107 3 279 715 405 3
Cuts at: 6795 1186
Size: 4463
Fragments arranged by size:
4463 1197 1039 715 648 626 405 321
279 138 107 49 27 12 10 9
3 3 3 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 2458 3732 4197 5624 5740 6312 6560 7140 7236
Size: 1274 465 1427 116 572 248 580 96
Cuts at: 7236 7248 9275 9771 2458
Size: 12 2027 496 2759
Fragments arranged by size:
2759 2027 1427 1274 580 572 496 465
248 116 96 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2754 3000 3195 5337 5558 5898 6472
Size: 573 1018 246 195 2142 221 340 574
Cuts at: 6472 6996 1163
Size: 524 4239
Fragments arranged by size:
4239 2142 1018 574 573 524 340 246
221 195
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3056 7522 8283 9311 1540
Size: 275 186 1055 4466 761 1028 2301
Fragments arranged by size:
4466 2301 1055 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6173 8133 8218 9289 6173
Size: 1960 85 1071 6956
Fragments arranged by size:
6956 1960 1071 85
BsiWI C'GTAC_G
Cuts at: 5977 5977
Size: 10072
BsmI GAATG_Cn'
Cuts at: 2591 2831 2921 2946 3116 3118 4285 4721 4798
Size: 240 90 25 170 2 1167 436 77
Cuts at: 4798 7583 7676 2591
Size: 2785 93 4987
Fragments arranged by size:
4987 2785 1167 436 240 170 93 90
77 25 2
BsmBI CGTCTCn'nnnn_
Cuts at: 3848 4592 6497 6942 3848
Size: 744 1905 445 6978
Fragments arranged by size:
6978 1905 744 445
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2575 3950 4623 5140 5613 6173 7235
Size: 399 785 1375 673 517 473 560 1062
Cuts at: 7235 7391 8133 8218 9289 1391
Size: 156 742 85 1071 2174
Fragments arranged by size:
2174 1375 1071 1062 785 742 673 560
517 473 399 156 85
BspEI T'CCGG_A
Cuts at: 9735 9735
Size: 10072
BspHI T'CATG_A
Cuts at: 3004 7961 9691 3004
Size: 4957 1730 3385
Fragments arranged by size:
4957 3385 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4261 4993 6389 7427 844
Size: 3417 732 1396 1038 3489
Fragments arranged by size:
3489 3417 1396 1038 732
BsrBI CCG'CTC
Cuts at: 1394 1823 4174 7959 1394
Size: 429 2351 3785 3507
Fragments arranged by size:
3785 3507 2351 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2338 3957 4780 6638 8567 8741 65
Size: 1183 1090 1619 823 1858 1929 174 1396
Fragments arranged by size:
1929 1858 1619 1396 1183 1090 823 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3297 4299 8716 8903 1700
Size: 110 1487 1002 4417 187 2869
Fragments arranged by size:
4417 2869 1487 1002 187 110
BsrGI T'GTAC_A
Cuts at: 96 4965 7287 96
Size: 4869 2322 2881
Fragments arranged by size:
4869 2881 2322
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10072
BssSI C'ACGA_G
Cuts at: 8132 9144 8132
Size: 1012 9060
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2476 2826 2835 6683 7258 794
Size: 1682 350 9 3848 575 3608
Fragments arranged by size:
3848 3608 1682 575 350 9
BstBI TT'CG_AA
Cuts at: 2007 8876 2007
Size: 6869 3203
BstEII G'GTnAC_C
Cuts at: 1184 3132 1184
Size: 1948 8124
BstXI CCAn_nnnn'nTGG
Cuts at: 6487 6487
Size: 10072
BstYI r'GATC_y
Cuts at: 1972 3104 5287 7402 7769 8154 8171 9623 9709
Size: 1132 2183 2115 367 385 17 1452 86
Cuts at: 9709 9721 10067 1972
Size: 12 346 1977
Fragments arranged by size:
2183 2115 1977 1452 1132 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5196 5196
Size: 10072
BtgI C'CryG_G
Cuts at: 513 1065 2022 2140 4986 5334 5748 7273 513
Size: 552 957 118 2846 348 414 1525 3312
Fragments arranged by size:
3312 2846 1525 957 552 414 348 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9333
Fragments arranged by size:
9333 683 56
BtsI GCAGTG_nn'
Cuts at: 2822 2845 4140 4357 4579 7588 8382 8402 2822
Size: 23 1295 217 222 3009 794 20 4492
Fragments arranged by size:
4492 3009 1295 794 222 217 23 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10072
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3439 3474 529
Size: 35 2875 35 7127
Fragments arranged by size:
7127 2875 35 35
DraI TTT'AAA
Cuts at: 4954 7376 4954
Size: 2422 7650
DraIII CAC_nnn'GTG
Cuts at: 5165 5165
Size: 10072
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5579 9079 809
Size: 507 4263 3500 1802
Fragments arranged by size:
4263 3500 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3295 4301 4889
Size: 54 1425 50 275 18 1465 1006 588
Cuts at: 4889 5968 7519 8408 10037 8
Size: 1079 1551 889 1629 43
Fragments arranged by size:
1629 1551 1465 1425 1079 1006 889 588
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7519 1537
Size: 275 5707 4090
Fragments arranged by size:
5707 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2103 3210 3581 3787 3860 4140 4356 4386
Size: 750 1107 371 206 73 280 216 30
Cuts at: 4386 4398 4404 4413 4425 4635 4755 5196 5202
Size: 12 6 9 12 210 120 441 6
Cuts at: 5202 5220 5229 5238 5367 5379 5388 5466 6247
Size: 18 9 9 129 12 9 78 781
Cuts at: 6247 8002 1353
Size: 1755 3423
Fragments arranged by size:
3423 1755 1107 781 750 441 371 280
216 210 206 129 120 78 73 30
18 12 12 12 9 9 9 9
6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8659 9033 9179 387
Size: 1064 138 7070 374 146 1280
Fragments arranged by size:
7070 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 2420 3234 3459 4161 4352 4682
Size: 393 495 293 814 225 702 191 330
Cuts at: 4682 5016 5655 6262 6574 8135 8732 9519 9787
Size: 334 639 607 312 1561 597 787 268
Cuts at: 9787 1239
Size: 1524
Fragments arranged by size:
1561 1524 814 787 702 639 607 597
495 393 334 330 312 293 268 225
191
EcoNI CCTnn'n_nnAGG
Cuts at: 3677 3677
Size: 10072
EcoO109I rG'GnC_Cy
Cuts at: 2100 2239 5629 6758 7007 2100
Size: 139 3390 1129 249 5165
Fragments arranged by size:
5165 3390 1129 249 139
EcoRI G'AATT_C
Cuts at: 3401 7381 7759 3401
Size: 3980 378 5714
Fragments arranged by size:
5714 3980 378
FspI TGC'GCA
Cuts at: 8578 8578
Size: 10072
HaeII r_GCGC'y
Cuts at: 1678 2260 6985 7070 9219 1678
Size: 582 4725 85 2149 2531
Fragments arranged by size:
4725 2531 2149 582 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3089 3121 3747 3779 3999
Size: 32 135 32 1517 32 626 32 220
Cuts at: 3999 4031 6089 6121 6210 6242 6790 6822 8713
Size: 32 2058 32 89 32 548 32 1891
Cuts at: 8713 8745 8787 8819 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2058 1891 1517 626 548 220 135
89 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4227 5584 5989 6615 7084 7416
Size: 988 281 2289 1357 405 626 469 332
Cuts at: 7416 7662 669
Size: 246 3079
Fragments arranged by size:
3079 2289 1357 988 626 469 405 332
281 246
HindIII A'AGCT_T
Cuts at: 748 3386 3686 5488 748
Size: 2638 300 1802 5332
Fragments arranged by size:
5332 2638 1802 300
HpaI GTT'AAC
Cuts at: 6615 7662 6615
Size: 1047 9025
KpnI G_GTAC'C
Cuts at: 3304 7397 3304
Size: 4093 5979
MfeI C'AATT_G
Cuts at: 2364 7671 2364
Size: 5307 4765
MluI A'CGCG_T
Cuts at: 9948 9948
Size: 10072
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2127 3646 5907 6348 6401 9186 9370 1305
Size: 822 1519 2261 441 53 2785 184 2007
Fragments arranged by size:
2785 2261 2007 1519 822 441 184 53
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4891 5970 10
Size: 54 1425 343 3059 1079 4112
Fragments arranged by size:
4112 3059 1425 1079 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3897 6056 6917 7043 7049 7100 7325
Size: 648 2731 2159 861 126 6 51 225
Cuts at: 7325 8030 8389 8548 518
Size: 705 359 159 2042
Fragments arranged by size:
2731 2159 2042 861 705 648 359 225
159 126 51 6
MspA1I CmG'CkG
Cuts at: 1606 2173 2473 4327 4660 6255 6300 6333 6442
Size: 567 300 1854 333 1595 45 33 109
Cuts at: 6442 6487 6659 6953 7488 8165 9313 9558 1606
Size: 45 172 294 535 677 1148 245 2120
Fragments arranged by size:
2120 1854 1595 1148 677 567 535 333
300 294 245 172 109 45 45 33
NaeI GCC'GGC
Cuts at: 4301 4301
Size: 10072
NcoI C'CATG_G
Cuts at: 513 1065 2022 2140 4986 5748 513
Size: 552 957 118 2846 762 4837
Fragments arranged by size:
4837 2846 957 762 552 118
NdeI CA'TA_TG
Cuts at: 387 2910 7243 387
Size: 2523 4333 3216
Fragments arranged by size:
4333 3216 2523
NgoMIV G'CCGG_C
Cuts at: 4299 4299
Size: 10072
NheI G'CTAG_C
Cuts at: 1052 4190 1052
Size: 3138 6934
NotI GC'GGCC_GC
Cuts at: 7519 7519
Size: 10072
NsiI A_TGCA'T
Cuts at: 3118 3118
Size: 10072
NspI r_CATG'y
Cuts at: 1132 1229 2838 3946 5136 6349 7138 7330 7430
Size: 97 1609 1108 1190 1213 789 192 100
Cuts at: 7430 8975 1132
Size: 1545 2229
Fragments arranged by size:
2229 1609 1545 1213 1190 1108 789 192
100 97
PasI CC'CwG_GG
Cuts at: 1348 2252 6752 1348
Size: 904 4500 4668
Fragments arranged by size:
4668 4500 904
PciI A'CATG_T
Cuts at: 5132 8971 5132
Size: 3839 6233
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2146 6442 1108
Size: 1038 4296 4738
Fragments arranged by size:
4738 4296 1038
PfoI T'CCnGG_A
Cuts at: 3579 3579
Size: 10072
PmeI GTTT'AAAC
Cuts at: 7376 7376
Size: 10072
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6242 6274 8141 8173 9654 9686 6242
Size: 32 1867 32 1481 32 6628
Fragments arranged by size:
6628 1867 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2239 5629 6758 7007 2239
Size: 3390 1129 249 5304
Fragments arranged by size:
5304 3390 1129 249
PshAI GACnn'nnGTC
Cuts at: 1613 5886 1613
Size: 4273 5799
PsiI TTA'TAA
Cuts at: 7642 7642
Size: 10072
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 7145 7177 7145
Size: 32 10040
PstI C_TGCA'G
Cuts at: 830 2975 3975 4135 6072 6344 6354 6386 6609
Size: 2145 1000 160 1937 272 10 32 223
Cuts at: 6609 7424 830
Size: 815 3478
Fragments arranged by size:
3478 2145 1937 1000 815 272 223 160
32 10
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10072
PvuII CAG'CTG
Cuts at: 2173 4327 4660 6255 6300 6442 6487 2173
Size: 2154 333 1595 45 142 45 5758
Fragments arranged by size:
5758 2154 1595 333 142 45 45
RsrII CG'GwC_CG
Cuts at: 2528 2528
Size: 10072
SalI G'TCGA_C
Cuts at: 1936 5582 7414 1936
Size: 3646 1832 4594
Fragments arranged by size:
4594 3646 1832
SanDI GG'GwC_CC
Cuts at: 5629 6758 5629
Size: 1129 8943
SapI GCTCTTCn'nnn_
Cuts at: 3581 3860 4140 5388 5466 6247 3581
Size: 279 280 1248 78 781 7406
Fragments arranged by size:
7406 1248 781 280 279 78
SbfI CC_TGCA'GG
Cuts at: 7424 7424
Size: 10072
ScaI AGT'ACT
Cuts at: 2134 4611 5502 8320 10032 2134
Size: 2477 891 2818 1712 2174
Fragments arranged by size:
2818 2477 2174 1712 891
SexAI A'CCwGG_T
Cuts at: 3341 5161 3341
Size: 1820 8252
SfcI C'TryA_G
Cuts at: 826 1045 2845 2971 3971 4131 5851 5861 6068
Size: 219 1800 126 1000 160 1720 10 207
Cuts at: 6068 6262 6340 6350 6382 6605 6993 7420 8555
Size: 194 78 10 32 223 388 427 1135
Cuts at: 8555 9236 9427 826
Size: 681 191 1471
Fragments arranged by size:
1800 1720 1471 1135 1000 681 427 388
223 219 207 194 191 160 126 78
32 10 10
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10072
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10072
SmaI CCC'GGG
Cuts at: 1872 7399 1872
Size: 5527 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2375 2806 5106 5841 6399 8176
Size: 197 926 432 431 2300 735 558 1777
Cuts at: 8176 9077 9339 820
Size: 901 262 1553
Fragments arranged by size:
2300 1777 1553 926 901 735 558 432
431 262 197
SnaBI TAC'GTA
Cuts at: 493 7369 7863 493
Size: 6876 494 2702
Fragments arranged by size:
6876 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10072
SphI G_CATG'C
Cuts at: 1132 6349 7430 1132
Size: 5217 1081 3774
Fragments arranged by size:
5217 3774 1081
SspI AAT'ATT
Cuts at: 5 52 3433 4280 4462 6859 7996 5
Size: 47 3381 847 182 2397 1137 2081
Fragments arranged by size:
3381 2397 2081 1137 847 182 47
StuI AGG'CCT
Cuts at: 1517 2223 2804 4522 5849 6421 1517
Size: 706 581 1718 1327 572 5168
Fragments arranged by size:
5168 1718 1327 706 581 572
StyI C'CwwG_G
Cuts at: 513 1065 2022 2140 2298 2854 4986 5613 5748
Size: 552 957 118 158 556 2132 627 135
Cuts at: 5748 7511 513
Size: 1763 3074
Fragments arranged by size:
3074 2132 1763 957 627 556 552 158
135 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2772 3070 4999 6326 6493 6974 7250
Size: 1255 1192 298 1929 1327 167 481 276
Cuts at: 7250 8099 8116 8269 8454 8899 325
Size: 849 17 153 185 445 1498
Fragments arranged by size:
1929 1498 1327 1255 1192 849 481 445
298 276 185 167 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2132 2535 4261
Size: 274 80 33 51 1443 155 403 1726
Cuts at: 4261 4528 4609 4965 5500 7287 8318 10030 96
Size: 267 81 356 535 1787 1031 1712 138
Fragments arranged by size:
1787 1726 1712 1443 1031 535 403 356
274 267 155 138 81 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6732 6764 10032 10064 6732
Size: 32 3268 32 6740
Fragments arranged by size:
6740 3268 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10072
XbaI T'CTAG_A
Cuts at: 2306 3788 7408 2306
Size: 1482 3620 4970
Fragments arranged by size:
4970 3620 1482
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2952 3577 4733 5963 6091 7172 2952
Size: 625 1156 1230 128 1081 5852
Fragments arranged by size:
5852 1230 1156 1081 625 128
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10072
XmaI C'CCGG_G
Cuts at: 1870 7397 1870
Size: 5527 4545
XmnI GAAnn'nnTTC
Cuts at: 3116 4461 8201 3116
Size: 1345 3740 4987
Fragments arranged by size:
4987 3740 1345
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7880 276
Size: 53 83 186 936 6346 2468
Fragments arranged by size:
6346 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII RsrII SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I
BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NarI
NruI PacI PmlI PspOMI PspXI SacI SacII SfiI
SfoI SrfI SwaI