(Circular) (Six-base) MAPSORT of: pFN21ASDA0380.seq Check: 7014 from: 1 to: 9295 pFN21ASDA0380 9295 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4278 4278 Size: 9295 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5213 7105 278 Size: 53 83 186 936 3677 1892 2468 Fragments arranged by size: 3677 2468 1892 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6638 1937 Size: 4701 4594 Acc65I G'GTAC_C Cuts at: 6616 6616 Size: 9295 AclI AA'CG_TT Cuts at: 1563 7422 7795 1563 Size: 5859 373 3063 Fragments arranged by size: 5859 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2993 4278 5057 5071 5967 6087 7358 8742 Size: 1361 1285 779 14 896 120 1271 1384 Cuts at: 8742 9010 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1361 1285 1271 896 779 268 120 14 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9098 AflIII A'CryG_T Cuts at: 1203 5376 8194 9171 1203 Size: 4173 2818 977 1327 Fragments arranged by size: 4173 2818 1327 977 AgeI A'CCGG_T Cuts at: 2855 8126 2855 Size: 5271 4024 AhdI GACnn_n'nnGTC Cuts at: 2145 8024 2145 Size: 5879 3416 AleI CACnn'nnGTG Cuts at: 1166 2309 2459 6410 1166 Size: 1143 150 3951 4051 Fragments arranged by size: 4051 3951 1143 150 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3402 3774 4524 1184 Size: 2218 372 750 5955 Fragments arranged by size: 5955 2218 750 372 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5493 5525 5493 Size: 32 9263 AlwNI CAG_nnn'CTG Cuts at: 2762 2962 3770 5458 6056 7038 8610 2762 Size: 200 808 1688 598 982 1572 3447 Fragments arranged by size: 3447 1688 1572 982 808 598 200 ApaI G_GGCC'C Cuts at: 4750 5180 5342 6519 4750 Size: 430 162 1177 7526 Fragments arranged by size: 7526 1177 430 162 ApaLI G'TGCA_C Cuts at: 7352 8508 7352 Size: 1156 8139 ApoI r'AATT_y Cuts at: 1493 5902 6604 6830 6982 9091 1493 Size: 4409 702 226 152 2109 1697 Fragments arranged by size: 4409 2109 1697 702 226 152 AseI AT'TA_AT Cuts at: 160 1032 7849 160 Size: 872 6817 1606 Fragments arranged by size: 6817 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9295 AvaI C'yCGr_G Cuts at: 1870 1943 2920 4335 4485 4749 5319 5711 6620 Size: 73 977 1415 150 264 570 392 909 Cuts at: 6620 1870 Size: 4545 Fragments arranged by size: 4545 1415 977 909 570 392 264 150 73 BamHI G'GATC_C Cuts at: 2533 3474 6625 6992 2533 Size: 941 3151 367 4836 Fragments arranged by size: 4836 3151 941 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2303 2357 4718 5271 6157 Size: 325 217 627 516 54 2361 553 886 Cuts at: 6157 6401 6616 8071 618 Size: 244 215 1455 1842 Fragments arranged by size: 2361 1842 1455 886 627 553 516 325 244 217 215 54 BanII G_rGCy'C Cuts at: 1391 2387 2418 2446 2834 4750 5066 5180 5241 Size: 996 31 28 388 1916 316 114 61 Cuts at: 5241 5342 5613 5712 6170 6480 6519 6614 1391 Size: 101 271 99 458 310 39 95 4072 Fragments arranged by size: 4072 1916 996 458 388 316 310 271 114 101 99 95 61 39 31 28 BbeI G_GCGC'C Cuts at: 2361 2361 Size: 9295 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2061 2916 3426 3473 5862 6558 928 Size: 971 162 855 510 47 2389 696 3665 Fragments arranged by size: 3665 2389 971 855 696 510 162 47 BbvCI CC'TCA_GC Cuts at: 2579 6000 6174 6210 6216 2579 Size: 3421 174 36 6 5658 Fragments arranged by size: 5658 3421 174 36 6 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7486 7520 1903 Size: 34 5549 34 3678 Fragments arranged by size: 5549 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7187 8397 7187 Size: 1210 8085 BclI T'GATC_A Cuts at: 1548 2346 2793 1548 Size: 798 447 8050 Fragments arranged by size: 8050 798 447 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2261 2807 3870 Size: 107 122 71 631 605 589 546 1063 Cuts at: 3870 7906 136 Size: 4036 1525 Fragments arranged by size: 4036 1525 1063 631 605 589 546 122 107 71 BglII A'GATC_T Cuts at: 5187 9290 5187 Size: 4103 5192 BlpI GC'TnA_GC Cuts at: 6712 6712 Size: 9295 Bme1580I G_kGCm'C Cuts at: 1790 3915 4750 5180 5342 6519 7356 8512 1790 Size: 2125 835 430 162 1177 837 1156 2573 Fragments arranged by size: 2573 2125 1177 1156 837 835 430 162 BmgBI CAC'GTC Cuts at: 1318 2457 5377 6286 1318 Size: 1139 2920 909 4327 Fragments arranged by size: 4327 2920 1139 909 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3027 3305 3514 Size: 416 53 83 399 24 1610 278 209 Cuts at: 3514 4065 5430 5513 6521 7026 7984 442 Size: 551 1365 83 1008 505 958 1753 Fragments arranged by size: 1753 1610 1365 1008 958 551 505 416 399 278 209 83 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9295 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4511 4629 6189 6386 6550 4511 Size: 118 1560 197 164 7256 Fragments arranged by size: 7256 1560 197 164 118 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2265 2402 2984 3149 3836 3976 4163 4481 Size: 1026 137 582 165 687 140 187 318 Cuts at: 4481 4709 4877 4963 5009 5433 5852 5907 6478 Size: 228 168 86 46 424 419 55 571 Cuts at: 6478 7955 1239 Size: 1477 2579 Fragments arranged by size: 2579 1477 1026 687 582 571 424 419 318 228 187 168 165 140 137 86 55 46 Bpu10I CC'TnA_GC Cuts at: 2579 5472 6000 6174 6210 6216 6471 2579 Size: 2893 528 174 36 6 255 5403 Fragments arranged by size: 5403 2893 528 255 174 36 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3219 3851 4034 4757 5084 6121 6308 6323 6356 Size: 632 183 723 327 1037 187 15 33 Cuts at: 6356 7420 8285 8583 3219 Size: 1064 865 298 3931 Fragments arranged by size: 3931 1064 1037 865 723 632 327 298 187 183 33 15 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2153 2632 3897 4014 4632 5870 7958 Size: 617 654 479 1265 117 618 1238 2088 Cuts at: 7958 882 Size: 2219 Fragments arranged by size: 2219 2088 1265 1238 654 618 617 479 117 BsaAI yAC'GTr Cuts at: 493 1204 4978 6592 7086 493 Size: 711 3774 1614 494 2702 Fragments arranged by size: 3774 2702 1614 711 494 BsaBI GATnn'nnATC Cuts at: 9289 9289 Size: 9295 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2358 5210 6247 6540 Size: 53 83 186 936 825 2852 1037 293 Cuts at: 6540 7102 7484 8119 275 Size: 562 382 635 1451 Fragments arranged by size: 2852 1451 1037 936 825 635 562 382 293 186 83 53 BsaWI w'CCGG_w Cuts at: 2283 2855 3791 7728 8126 8400 8547 8958 2283 Size: 572 936 3937 398 274 147 411 2620 Fragments arranged by size: 3937 2620 936 572 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2361 2391 5759 5789 1178 Size: 30 165 30 958 30 3368 30 4684 Fragments arranged by size: 4684 3368 958 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2472 4355 5056 5417 6252 1186 Size: 1286 1883 701 361 835 4229 Fragments arranged by size: 4229 1883 1286 835 701 361 BseYI C'CCAG_C Cuts at: 2434 2811 3301 3470 4199 4209 5173 5331 5348 Size: 377 490 169 729 10 964 158 17 Cuts at: 5348 5427 5817 6251 8498 8994 2434 Size: 79 390 434 2247 496 2735 Fragments arranged by size: 2735 2247 964 729 496 490 434 390 377 169 158 79 17 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2070 2249 2271 3078 3950 4069 4494 Size: 573 334 179 22 807 872 119 425 Cuts at: 4494 5146 5508 6012 1163 Size: 652 362 504 4446 Fragments arranged by size: 4446 872 807 652 573 504 425 362 334 179 119 22 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2284 3792 6745 7506 8534 1540 Size: 275 186 283 1508 2953 761 1028 2301 Fragments arranged by size: 2953 2301 1508 1028 761 283 275 186 BsiHKAI G_wGCw'C Cuts at: 2446 5100 5118 5241 7356 7441 8512 2446 Size: 2654 18 123 2115 85 1071 3229 Fragments arranged by size: 3229 2654 2115 1071 123 85 18 BsmI GAATG_Cn' Cuts at: 5266 6806 6899 5266 Size: 1540 93 7662 Fragments arranged by size: 7662 1540 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2387 2418 2446 2834 3915 4750 5066 Size: 399 597 31 28 388 1081 835 316 Cuts at: 5066 5100 5118 5180 5241 5342 5613 5712 6170 Size: 34 18 62 61 101 271 99 458 Cuts at: 6170 6480 6519 6614 7356 7441 8512 1391 Size: 310 39 95 742 85 1071 2174 Fragments arranged by size: 2174 1081 1071 835 742 597 458 399 388 316 310 271 101 99 95 85 62 61 39 34 31 28 18 BspEI T'CCGG_A Cuts at: 8958 8958 Size: 9295 BspHI T'CATG_A Cuts at: 7184 8914 7184 Size: 1730 7565 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3336 3819 4278 4374 5652 6650 844 Size: 2492 483 459 96 1278 998 3489 Fragments arranged by size: 3489 2492 1278 998 483 459 96 BsrBI CCG'CTC Cuts at: 1394 1823 3855 3918 5124 5624 6258 7182 1394 Size: 429 2032 63 1206 500 634 924 3507 Fragments arranged by size: 3507 2032 1206 924 634 500 429 63 BsrDI GCAATG_nn' Cuts at: 65 1248 3718 7790 7964 65 Size: 1183 2470 4072 174 1396 Fragments arranged by size: 4072 2470 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2264 2652 2855 4224 7939 8126 1700 Size: 110 454 388 203 1369 3715 187 2869 Fragments arranged by size: 3715 2869 1369 454 388 203 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9295 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9295 BssSI C'ACGA_G Cuts at: 7355 8367 7355 Size: 1012 8283 BstAPI GCAn_nnn'nTGC Cuts at: 794 2236 794 Size: 1442 7853 BstBI TT'CG_AA Cuts at: 2007 8099 2007 Size: 6092 3203 BstEII G'GTnAC_C Cuts at: 1184 2034 2311 2858 1184 Size: 850 277 547 7621 Fragments arranged by size: 7621 850 547 277 BstXI CCAn_nnnn'nTGG Cuts at: 4855 5533 5578 6412 4855 Size: 678 45 834 7738 Fragments arranged by size: 7738 834 678 45 BstYI r'GATC_y Cuts at: 1972 2533 2562 2959 3156 3255 3267 3474 4900 Size: 561 29 397 197 99 12 207 1426 Cuts at: 4900 5187 6625 6992 7377 7394 8846 8932 8944 Size: 287 1438 367 385 17 1452 86 12 Cuts at: 8944 9290 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1438 1426 561 397 385 367 346 287 207 197 99 86 29 17 12 12 Bsu36I CC'TnA_GG Cuts at: 2567 3804 5400 6278 6525 2567 Size: 1237 1596 878 247 5337 Fragments arranged by size: 5337 1596 1237 878 247 BtgI C'CryG_G Cuts at: 513 1065 2307 2400 3214 3435 5383 5685 513 Size: 552 1242 93 814 221 1948 302 4123 Fragments arranged by size: 4123 1948 1242 814 552 302 221 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2099 487 Size: 683 56 873 7683 Fragments arranged by size: 7683 873 683 56 BtsI GCAGTG_nn' Cuts at: 3038 3931 5491 6811 7605 7625 3038 Size: 893 1560 1320 794 20 4708 Fragments arranged by size: 4708 1560 1320 893 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9295 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9260 DraI TTT'AAA Cuts at: 6599 6599 Size: 9295 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8302 809 Size: 507 6986 1802 Fragments arranged by size: 6986 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2799 2981 5130 Size: 54 1425 50 275 18 969 182 2149 Cuts at: 5130 5843 6043 6742 7631 9260 8 Size: 713 200 699 889 1629 43 Fragments arranged by size: 2149 1629 1425 969 889 713 699 275 200 182 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6742 1537 Size: 275 4930 4090 Fragments arranged by size: 4930 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2392 3566 3664 3873 4361 4592 5241 5490 Size: 1039 1174 98 209 488 231 649 249 Cuts at: 5490 7225 1353 Size: 1735 3423 Fragments arranged by size: 3423 1735 1174 1039 649 488 249 231 209 98 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3041 6093 7882 8256 8402 387 Size: 1064 138 1452 3052 1789 374 146 1280 Fragments arranged by size: 3052 1789 1452 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2444 5239 2444 Size: 2795 6500 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2265 2402 2984 2993 3149 3836 3976 Size: 393 633 137 582 9 156 687 140 Cuts at: 3976 4163 4278 4481 4709 4877 4963 5009 5057 Size: 187 115 203 228 168 86 46 48 Cuts at: 5057 5071 5433 5852 5907 5967 6087 6478 7358 Size: 14 362 419 55 60 120 391 880 Cuts at: 7358 7955 8742 9010 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 880 787 687 633 597 582 419 393 391 362 268 228 203 187 168 156 140 137 120 115 86 60 55 48 46 14 9 EcoNI CCTnn'n_nnAGG Cuts at: 4112 5462 4112 Size: 1350 7945 EcoO109I rG'GnC_Cy Cuts at: 2425 2516 3346 3808 4285 4430 4931 4993 5315 Size: 91 830 462 477 145 501 62 322 Cuts at: 5315 5338 5339 5424 5455 5467 5591 5767 6125 Size: 23 1 85 31 12 124 176 358 Cuts at: 6125 6515 6516 2425 Size: 390 1 5204 Fragments arranged by size: 5204 830 501 477 462 390 358 322 176 145 124 91 85 62 31 23 12 1 1 EcoRI G'AATT_C Cuts at: 6604 6982 6604 Size: 378 8917 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3619 5101 3619 Size: 1482 7813 FspI TGC'GCA Cuts at: 4274 7801 4274 Size: 3527 5768 HaeII r_GCGC'y Cuts at: 1678 2361 3364 3432 8442 1678 Size: 683 1003 68 5010 2531 Fragments arranged by size: 5010 2531 1003 683 68 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2084 2116 2131 2163 3141 Size: 32 135 32 512 32 15 32 978 Cuts at: 3141 3173 3389 3421 4569 4601 4629 4661 4885 Size: 32 216 32 1148 32 28 32 224 Cuts at: 4885 4917 5640 5672 5759 5791 6193 6225 7936 Size: 32 723 32 87 32 402 32 1711 Cuts at: 7936 7968 8010 8042 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1711 1148 978 723 512 402 224 216 135 87 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 28 15 HincII GTy'rAC Cuts at: 669 1657 1938 2299 3934 6639 6885 669 Size: 988 281 361 1635 2705 246 3079 Fragments arranged by size: 3079 2705 1635 988 361 281 246 HindIII A'AGCT_T Cuts at: 748 3097 3796 4260 748 Size: 2349 699 464 5783 Fragments arranged by size: 5783 2349 699 464 HpaI GTT'AAC Cuts at: 6885 6885 Size: 9295 KasI G'GCGC_C Cuts at: 2357 2357 Size: 9295 KpnI G_GTAC'C Cuts at: 6620 6620 Size: 9295 MfeI C'AATT_G Cuts at: 6894 6894 Size: 9295 MluI A'CGCG_T Cuts at: 9171 9171 Size: 9295 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3525 8409 8593 1305 Size: 2220 4884 184 2007 Fragments arranged by size: 4884 2220 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2801 5132 6045 10 Size: 54 1425 343 969 2331 913 3260 Fragments arranged by size: 3260 2331 1425 969 913 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2309 2459 5231 6410 7253 7612 7771 Size: 648 1143 150 2772 1179 843 359 159 Cuts at: 7771 518 Size: 2042 Fragments arranged by size: 2772 2042 1179 1143 843 648 359 159 150 MspA1I CmG'CkG Cuts at: 1606 3382 3509 4132 4213 4633 4663 4681 6220 Size: 1776 127 623 81 420 30 18 1539 Cuts at: 6220 6711 7388 8536 8781 1606 Size: 491 677 1148 245 2120 Fragments arranged by size: 2120 1776 1539 1148 677 623 491 420 245 127 81 30 18 NaeI GCC'GGC Cuts at: 2654 2654 Size: 9295 NarI GG'CG_CC Cuts at: 2358 2358 Size: 9295 NcoI C'CATG_G Cuts at: 513 1065 2307 2400 3435 5383 5685 513 Size: 552 1242 93 1035 1948 302 4123 Fragments arranged by size: 4123 1948 1242 1035 552 302 93 NdeI CA'TA_TG Cuts at: 387 387 Size: 9295 NgoMIV G'CCGG_C Cuts at: 2652 2652 Size: 9295 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9295 NotI GC'GGCC_GC Cuts at: 6742 6742 Size: 9295 NspI r_CATG'y Cuts at: 1132 1229 2555 6118 6653 8198 1132 Size: 97 1326 3563 535 1545 2229 Fragments arranged by size: 3563 2229 1545 1326 535 97 PasI CC'CwG_GG Cuts at: 1348 2762 5763 5780 5795 1348 Size: 1414 3001 17 15 4848 Fragments arranged by size: 4848 3001 1414 17 15 PciI A'CATG_T Cuts at: 8194 8194 Size: 9295 PflMI CCAn_nnn'nTGG Cuts at: 1108 2621 2962 3770 4504 6041 6511 1108 Size: 1513 341 808 734 1537 470 3892 Fragments arranged by size: 3892 1537 1513 808 734 470 341 PfoI T'CCnGG_A Cuts at: 2955 3126 4287 4419 6494 2955 Size: 171 1161 132 2075 5756 Fragments arranged by size: 5756 2075 1161 171 132 PmeI GTTT'AAAC Cuts at: 6599 6599 Size: 9295 PmlI CAC'GTG Cuts at: 4978 4978 Size: 9295 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3811 3843 7364 7396 8877 8909 3811 Size: 32 3521 32 1481 32 4197 Fragments arranged by size: 4197 3521 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2425 2516 3346 3808 4285 4931 4993 5315 5424 Size: 91 830 462 477 646 62 322 109 Cuts at: 5424 5455 5467 5767 6125 2425 Size: 31 12 300 358 5595 Fragments arranged by size: 5595 830 646 477 462 358 322 300 109 91 62 31 12 PshAI GACnn'nnGTC Cuts at: 1613 3033 5862 1613 Size: 1420 2829 5046 Fragments arranged by size: 5046 2829 1420 PsiI TTA'TAA Cuts at: 6865 6865 Size: 9295 PspOMI G'GGCC_C Cuts at: 4746 5176 5338 6515 4746 Size: 430 162 1177 7526 Fragments arranged by size: 7526 1177 430 162 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 6073 6105 6073 Size: 32 9263 PstI C_TGCA'G Cuts at: 830 2532 4632 6196 6225 6647 830 Size: 1702 2100 1564 29 422 3478 Fragments arranged by size: 3478 2100 1702 1564 422 29 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9295 PvuII CAG'CTG Cuts at: 3382 3509 4132 4633 6220 3382 Size: 127 623 501 1587 6457 Fragments arranged by size: 6457 1587 623 501 127 SacI G_AGCT'C Cuts at: 2446 5241 2446 Size: 2795 6500 SalI G'TCGA_C Cuts at: 1936 6637 1936 Size: 4701 4594 SanDI GG'GwC_CC Cuts at: 3346 4931 5424 5767 3346 Size: 1585 493 343 6874 Fragments arranged by size: 6874 1585 493 343 SapI GCTCTTCn'nnn_ Cuts at: 2392 3566 3664 4361 4592 2392 Size: 1174 98 697 231 7095 Fragments arranged by size: 7095 1174 697 231 98 SbfI CC_TGCA'GG Cuts at: 6196 6647 6196 Size: 451 8844 ScaI AGT'ACT Cuts at: 7543 9255 7543 Size: 1712 7583 SexAI A'CCwGG_T Cuts at: 4004 5451 4004 Size: 1447 7848 SfcI C'TryA_G Cuts at: 826 1045 2528 3170 3277 4628 5009 6192 6221 Size: 219 1483 642 107 1351 381 1183 29 Cuts at: 6221 6643 7778 8459 8650 826 Size: 422 1135 681 191 1471 Fragments arranged by size: 1483 1471 1351 1183 1135 681 642 422 381 219 191 107 29 SfiI GGCCn_nnn'nGGCC Cuts at: 2807 2807 Size: 9295 SfoI GGC'GCC Cuts at: 2359 2359 Size: 9295 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9295 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9295 SmaI CCC'GGG Cuts at: 1872 2922 4337 4751 5321 6622 1872 Size: 1050 1415 414 570 1301 4545 Fragments arranged by size: 4545 1415 1301 1050 570 414 SmlI C'TyrA_G Cuts at: 820 1017 1943 3234 3866 4013 4772 5099 6136 Size: 197 926 1291 632 147 759 327 1037 Cuts at: 6136 6323 6338 6371 7399 8300 8562 820 Size: 187 15 33 1028 901 262 1553 Fragments arranged by size: 1553 1291 1037 1028 926 901 759 632 327 262 197 187 147 33 15 SnaBI TAC'GTA Cuts at: 493 6592 7086 493 Size: 6099 494 2702 Fragments arranged by size: 6099 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9295 SphI G_CATG'C Cuts at: 1132 6118 6653 1132 Size: 4986 535 3774 Fragments arranged by size: 4986 3774 535 SspI AAT'ATT Cuts at: 5 52 3121 7219 5 Size: 47 3069 4098 2081 Fragments arranged by size: 4098 3069 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9295 StyI C'CwwG_G Cuts at: 513 1065 2307 2400 3084 3391 3435 3603 4965 Size: 552 1242 93 684 307 44 168 1362 Cuts at: 4965 5383 5440 5685 5891 6379 6734 513 Size: 418 57 245 206 488 355 3074 Fragments arranged by size: 3074 1362 1242 684 552 488 418 355 307 245 206 168 93 57 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2573 5362 5495 5743 5831 6175 7322 Size: 1255 993 2789 133 248 88 344 1147 Cuts at: 7322 7339 7492 7677 8122 325 Size: 17 153 185 445 1498 Fragments arranged by size: 2789 1498 1255 1147 993 445 344 248 185 153 133 88 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 7541 9253 96 Size: 274 80 33 51 1443 5564 1712 138 Fragments arranged by size: 5564 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2361 2393 4269 4301 9255 9287 2361 Size: 32 1876 32 4954 32 2369 Fragments arranged by size: 4954 2369 1876 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9295 XbaI T'CTAG_A Cuts at: 6631 6631 Size: 9295 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4873 6053 4873 Size: 1180 8115 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9295 XmaI C'CCGG_G Cuts at: 1870 2920 4335 4749 5319 6620 1870 Size: 1050 1415 414 570 1301 4545 Fragments arranged by size: 4545 1415 1301 1050 570 414 XmnI GAAnn'nnTTC Cuts at: 2709 3122 7424 2709 Size: 413 4302 4580 Fragments arranged by size: 4580 4302 413 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5211 7103 276 Size: 53 83 186 936 3677 1892 2468 Fragments arranged by size: 3677 2468 1892 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PspXI RsrII SacII SrfI SwaI