(Circular) (Six-base) MAPSORT of: pFN21ASDA0379.seq Check: 3353 from: 1 to: 7888 pFN21ASDA0379 7888 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5698 278 Size: 53 83 186 936 4162 2468 Fragments arranged by size: 4162 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3247 5231 1937 Size: 1310 1984 4594 Fragments arranged by size: 4594 1984 1310 Acc65I G'GTAC_C Cuts at: 2401 5209 2401 Size: 2808 5080 AclI AA'CG_TT Cuts at: 1563 6015 6388 1563 Size: 4452 373 3063 Fragments arranged by size: 4452 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2412 2634 3186 3569 5951 7335 7603 1632 Size: 780 222 552 383 2382 1384 268 1917 Fragments arranged by size: 2382 1917 1384 780 552 383 268 222 AflII C'TTAA_G Cuts at: 820 1017 3163 820 Size: 197 2146 5545 Fragments arranged by size: 5545 2146 197 AflIII A'CryG_T Cuts at: 1203 4331 6787 7764 1203 Size: 3128 2456 977 1327 Fragments arranged by size: 3128 2456 1327 977 AgeI A'CCGG_T Cuts at: 6719 6719 Size: 7888 AhdI GACnn_n'nnGTC Cuts at: 6617 6617 Size: 7888 AleI CACnn'nnGTG Cuts at: 1166 4218 5144 1166 Size: 3052 926 3910 Fragments arranged by size: 3910 3052 926 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2874 3372 3461 4836 1184 Size: 1690 498 89 1375 4236 Fragments arranged by size: 4236 1690 1375 498 89 AlwNI CAG_nnn'CTG Cuts at: 3382 4546 5631 7203 3382 Size: 1164 1085 1572 4067 Fragments arranged by size: 4067 1572 1164 1085 ApaLI G'TGCA_C Cuts at: 5945 7101 5945 Size: 1156 6732 ApoI r'AATT_y Cuts at: 1493 3271 3329 5197 5423 5575 7684 1493 Size: 1778 58 1868 226 152 2109 1697 Fragments arranged by size: 2109 1868 1778 1697 226 152 58 AseI AT'TA_AT Cuts at: 160 1032 2520 3831 3929 6442 160 Size: 872 1488 1311 98 2513 1606 Fragments arranged by size: 2513 1606 1488 1311 872 98 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7888 AvaI C'yCGr_G Cuts at: 1870 1943 2328 5213 1870 Size: 73 385 2885 4545 Fragments arranged by size: 4545 2885 385 73 BamHI G'GATC_C Cuts at: 3519 5218 5585 3519 Size: 1699 367 5822 Fragments arranged by size: 5822 1699 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2401 2510 4457 5209 6664 Size: 325 217 627 614 109 1947 752 1455 Cuts at: 6664 618 Size: 1842 Fragments arranged by size: 1947 1842 1455 752 627 614 325 217 109 BanII G_rGCy'C Cuts at: 1391 4234 5157 5207 1391 Size: 2843 923 50 4072 Fragments arranged by size: 4072 2843 923 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3575 928 Size: 971 1676 5241 Fragments arranged by size: 5241 1676 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4412 4446 6079 6113 1903 Size: 34 2475 34 1633 34 3678 Fragments arranged by size: 3678 2475 1633 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3530 5780 6990 3530 Size: 2250 1210 4428 Fragments arranged by size: 4428 2250 1210 BclI T'GATC_A Cuts at: 1548 2676 3084 1548 Size: 1128 408 6352 Fragments arranged by size: 6352 1128 408 BfrBI ATG'CAT Cuts at: 4133 4133 Size: 7888 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6499 136 Size: 107 122 71 631 605 4827 1525 Fragments arranged by size: 4827 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2701 3338 3745 3784 7883 2701 Size: 637 407 39 4099 2706 Fragments arranged by size: 4099 2706 637 407 39 BlpI GC'TnA_GC Cuts at: 4550 5305 4550 Size: 755 7133 Bme1580I G_kGCm'C Cuts at: 1790 4017 5949 7105 1790 Size: 2227 1932 1156 2573 Fragments arranged by size: 2573 2227 1932 1156 BmgBI CAC'GTC Cuts at: 1318 5146 1318 Size: 3828 4060 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4315 5619 6577 Size: 416 53 83 399 24 2898 1304 958 Cuts at: 6577 442 Size: 1753 Fragments arranged by size: 2898 1753 1304 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7888 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2232 3285 6548 1239 Size: 993 1053 3263 2579 Fragments arranged by size: 3263 2579 1053 993 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4548 6013 6878 7176 4548 Size: 1465 865 298 5260 Fragments arranged by size: 5260 1465 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5168 6551 882 Size: 617 3669 1383 2219 Fragments arranged by size: 3669 2219 1383 617 BsaAI yAC'GTr Cuts at: 493 1204 5185 5679 493 Size: 711 3981 494 2702 Fragments arranged by size: 3981 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3510 4263 7882 3510 Size: 753 3619 3516 Fragments arranged by size: 3619 3516 753 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3986 4184 5695 6077 Size: 53 83 186 936 2453 198 1511 382 Cuts at: 6077 6712 275 Size: 635 1451 Fragments arranged by size: 2453 1511 1451 936 635 382 198 186 83 53 BsaWI w'CCGG_w Cuts at: 6321 6719 6993 7140 7551 6321 Size: 398 274 147 411 6658 Fragments arranged by size: 6658 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2205 2235 2601 2631 3396 Size: 30 165 30 802 30 366 30 765 Cuts at: 3396 3426 4041 4071 4860 4890 1178 Size: 30 615 30 789 30 4176 Fragments arranged by size: 4176 802 789 765 615 366 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3597 4488 1186 Size: 2411 891 4586 Fragments arranged by size: 4586 2411 891 BseYI C'CCAG_C Cuts at: 4906 7091 7587 4906 Size: 2185 496 5207 Fragments arranged by size: 5207 2185 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3327 3332 3421 3431 4683 1163 Size: 573 1591 5 89 10 1252 4368 Fragments arranged by size: 4368 1591 1252 573 89 10 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5338 6099 7127 1540 Size: 275 186 3337 761 1028 2301 Fragments arranged by size: 3337 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2101 4924 5157 5949 6034 7105 2101 Size: 2823 233 792 85 1071 2884 Fragments arranged by size: 2884 2823 1071 792 233 85 BsmI GAATG_Cn' Cuts at: 2308 3920 3958 5399 5492 2308 Size: 1612 38 1441 93 4704 Fragments arranged by size: 4704 1612 1441 93 38 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2101 4017 4234 4924 5157 5207 5949 Size: 399 311 1916 217 690 233 50 742 Cuts at: 5949 6034 7105 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1916 1071 742 690 399 311 233 217 85 50 BspEI T'CCGG_A Cuts at: 7551 7551 Size: 7888 BspHI T'CATG_A Cuts at: 4726 5073 5777 7507 4726 Size: 347 704 1730 5107 Fragments arranged by size: 5107 1730 704 347 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5243 844 Size: 4399 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5775 1394 Size: 429 3952 3507 Fragments arranged by size: 3952 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2153 2911 4034 4394 6383 6557 65 Size: 1183 905 758 1123 360 1989 174 1396 Fragments arranged by size: 1989 1396 1183 1123 905 758 360 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6532 6719 1700 Size: 110 4722 187 2869 Fragments arranged by size: 4722 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7888 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7888 BssSI C'ACGA_G Cuts at: 4325 5948 6960 4325 Size: 1623 1012 5253 Fragments arranged by size: 5253 1623 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7888 BstBI TT'CG_AA Cuts at: 2007 6692 2007 Size: 4685 3203 BstEII G'GTnAC_C Cuts at: 1184 3569 3704 1184 Size: 2385 135 5368 Fragments arranged by size: 5368 2385 135 BstYI r'GATC_y Cuts at: 1972 2080 2701 3338 3519 3745 3784 5218 5585 Size: 108 621 637 181 226 39 1434 367 Cuts at: 5585 5970 5987 7439 7525 7537 7883 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1434 637 621 385 367 346 226 181 108 86 39 17 12 Bsu36I CC'TnA_GG Cuts at: 3631 3631 Size: 7888 BtgI C'CryG_G Cuts at: 513 1065 2022 2968 513 Size: 552 957 946 5433 Fragments arranged by size: 5433 957 946 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7149 Fragments arranged by size: 7149 683 56 BtsI GCAGTG_nn' Cuts at: 3114 3344 3963 4660 5404 6198 6218 3114 Size: 230 619 697 744 794 20 4784 Fragments arranged by size: 4784 794 744 697 619 230 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7888 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7853 DraI TTT'AAA Cuts at: 5192 5192 Size: 7888 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6895 809 Size: 507 5579 1802 Fragments arranged by size: 5579 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3070 4962 5335 Size: 54 1425 50 275 18 1240 1892 373 Cuts at: 5335 6224 7853 8 Size: 889 1629 43 Fragments arranged by size: 1892 1629 1425 1240 889 373 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5335 1537 Size: 275 3523 4090 Fragments arranged by size: 4090 3523 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2412 2655 2754 2921 3866 5818 1353 Size: 1059 243 99 167 945 1952 3423 Fragments arranged by size: 3423 1952 1059 945 243 167 99 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6475 6849 6995 387 Size: 1064 138 4886 374 146 1280 Fragments arranged by size: 4886 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5155 5155 Size: 7888 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2232 2412 2634 3186 3285 3569 5951 Size: 393 600 180 222 552 99 284 2382 Cuts at: 5951 6548 7335 7603 1239 Size: 597 787 268 1524 Fragments arranged by size: 2382 1524 787 600 597 552 393 284 268 222 180 99 EcoRI G'AATT_C Cuts at: 5197 5575 5197 Size: 378 7510 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5010 5010 Size: 7888 FspI TGC'GCA Cuts at: 6394 6394 Size: 7888 HaeII r_GCGC'y Cuts at: 1678 7035 1678 Size: 5357 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2036 2068 2621 2653 3000 Size: 32 135 32 464 32 553 32 347 Cuts at: 3000 3032 3076 3108 3831 3863 6529 6561 6603 Size: 32 44 32 723 32 2666 32 42 Cuts at: 6603 6635 1373 Size: 32 2626 Fragments arranged by size: 2666 2626 723 553 464 347 135 44 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2125 2247 5232 5478 669 Size: 988 281 187 122 2985 246 3079 Fragments arranged by size: 3079 2985 988 281 246 187 122 HindIII A'AGCT_T Cuts at: 748 2394 5061 748 Size: 1646 2667 3575 Fragments arranged by size: 3575 2667 1646 HpaI GTT'AAC Cuts at: 2125 5478 2125 Size: 3353 4535 KpnI G_GTAC'C Cuts at: 2405 5213 2405 Size: 2808 5080 MfeI C'AATT_G Cuts at: 5487 5487 Size: 7888 MluI A'CGCG_T Cuts at: 7764 7764 Size: 7888 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7002 7186 1305 Size: 5697 184 2007 Fragments arranged by size: 5697 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3072 4964 10 Size: 54 1425 343 1240 1892 2934 Fragments arranged by size: 2934 1892 1425 1240 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4218 4970 5144 5846 6205 6364 518 Size: 648 3052 752 174 702 359 159 2042 Fragments arranged by size: 3052 2042 752 702 648 359 174 159 MspA1I CmG'CkG Cuts at: 1606 3257 3263 3560 3589 4543 5304 5981 7129 Size: 1651 6 297 29 954 761 677 1148 Cuts at: 7129 7374 1606 Size: 245 2120 Fragments arranged by size: 2120 1651 1148 954 761 677 297 245 29 6 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 6379 Fragments arranged by size: 6379 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7888 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7888 NotI GC'GGCC_GC Cuts at: 5335 5335 Size: 7888 NsiI A_TGCA'T Cuts at: 4135 4135 Size: 7888 NspI r_CATG'y Cuts at: 1132 1229 2625 3254 3646 4335 4817 5246 6791 Size: 97 1396 629 392 689 482 429 1545 Cuts at: 6791 1132 Size: 2229 Fragments arranged by size: 2229 1545 1396 689 629 482 429 392 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7888 PciI A'CATG_T Cuts at: 4331 6787 4331 Size: 2456 5432 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7888 PmeI GTTT'AAAC Cuts at: 5192 5192 Size: 7888 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5957 5989 7470 7502 5957 Size: 32 1481 32 6343 Fragments arranged by size: 6343 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7888 PsiI TTA'TAA Cuts at: 5458 5458 Size: 7888 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4659 4691 4659 Size: 32 7856 PstI C_TGCA'G Cuts at: 830 2317 3188 3262 4615 5240 830 Size: 1487 871 74 1353 625 3478 Fragments arranged by size: 3478 1487 1353 871 625 74 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7888 PvuII CAG'CTG Cuts at: 3257 3263 3560 3589 4543 3257 Size: 6 297 29 954 6602 Fragments arranged by size: 6602 954 297 29 6 SacI G_AGCT'C Cuts at: 5157 5157 Size: 7888 SalI G'TCGA_C Cuts at: 1936 5230 1936 Size: 3294 4594 SbfI CC_TGCA'GG Cuts at: 5240 5240 Size: 7888 ScaI AGT'ACT Cuts at: 5132 6136 7848 5132 Size: 1004 1712 5172 Fragments arranged by size: 5172 1712 1004 SfcI C'TryA_G Cuts at: 826 1045 2313 3184 3258 4584 4611 5236 6371 Size: 219 1268 871 74 1326 27 625 1135 Cuts at: 6371 7052 7243 826 Size: 681 191 1471 Fragments arranged by size: 1471 1326 1268 1135 871 681 625 219 191 74 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7888 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7888 SmaI CCC'GGG Cuts at: 1872 5215 1872 Size: 3343 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2328 3163 4563 5992 6893 7155 Size: 197 926 385 835 1400 1429 901 262 Cuts at: 7155 820 Size: 1553 Fragments arranged by size: 1553 1429 1400 926 901 835 385 262 197 SnaBI TAC'GTA Cuts at: 493 5185 5679 493 Size: 4692 494 2702 Fragments arranged by size: 4692 2702 494 SpeI A'CTAG_T Cuts at: 152 4733 152 Size: 4581 3307 SphI G_CATG'C Cuts at: 1132 5246 1132 Size: 4114 3774 SspI AAT'ATT Cuts at: 5 52 2106 2518 5812 5 Size: 47 2054 412 3294 2081 Fragments arranged by size: 3294 2081 2054 412 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7888 StyI C'CwwG_G Cuts at: 513 1065 2022 2173 5327 513 Size: 552 957 151 3154 3074 Fragments arranged by size: 3154 3074 957 552 151 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5915 5932 6085 6270 6715 325 Size: 1255 4335 17 153 185 445 1498 Fragments arranged by size: 4335 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3823 5130 6134 Size: 274 80 33 51 1443 1846 1307 1004 Cuts at: 6134 7846 96 Size: 1712 138 Fragments arranged by size: 1846 1712 1443 1307 1004 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2601 2633 3396 3428 4860 4892 7848 7880 2601 Size: 32 763 32 1432 32 2956 32 2609 Fragments arranged by size: 2956 2609 1432 763 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7888 XbaI T'CTAG_A Cuts at: 2504 2698 3610 5224 2504 Size: 194 912 1614 5168 Fragments arranged by size: 5168 1614 912 194 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4933 4933 Size: 7888 XhoI C'TCGA_G Cuts at: 1943 2328 1943 Size: 385 7503 XmaI C'CCGG_G Cuts at: 1870 5213 1870 Size: 3343 4545 XmnI GAAnn'nnTTC Cuts at: 2289 6017 2289 Size: 3728 4160 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5696 276 Size: 53 83 186 936 4162 2468 Fragments arranged by size: 4162 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI