Restriction map for
pFN21ASDA0359
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BbrPI BbvCI BciVI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FbaI FseI
FspI HgaI HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NciI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 638 CGCG CG^CG
AcsI 1069 GAATTC [GA]^AATT[CT]
AcyI 1390 GGCGCC G[GA]^CG[CT]C
AfaI 216 GTAC GT^AC
AocI 2186 CCTCAGG CC^T[ACGT]AGG
ApoI 1069 GAATTC [GA]^AATT[CT]
AspI 244 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2222 CTCGGG C^[CT]CG[GA]G
BalI 941 TGGCCA TGG^CCA
BamHI 2170 GGATCC G^GATCC
BanI 1390 GGCGCC G^G[CT][GA]CC
BbeI 1390 GGCGCC GGCGC^C
BbsI 537 GTCTTC GAAGAC(2/6)
BcgI 1503 GCAGAAACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 537 GTCTTC GAAGAC(2/6)
BsaHI 1390 GGCGCC G[GA]^CG[CT]C
BsgI 672 GTGCAG GTGCAG(16/14)
BsmBI 223 GAGACG CGTCTC(1/5)
BsmI 195 GAATGC GAATGC(1/-1)
BsoBI 2222 CTCGGG C^[CT]CG[GA]G
BsrFI 763 ACCGGC [GA]^CCGG[CT]
BstAPI 2059 GCATTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 331 GGTGACC G^GT[ACGT]ACC
BstPI 331 GGTGACC G^GT[ACGT]ACC
BstUI 638 CGCG CG^CG
Bsu36I 2186 CCTCAGG CC^T[ACGT]AGG
Cfr10I 763 ACCGGC [GA]^CCGG[CT]
Eco81I 2186 CCTCAGG CC^T[ACGT]AGG
EcoNI 508 CCTGACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 331 GGTGACC G^GT[ACGT]ACC
EcoRI 1069 GAATTC G^AATTC
EcoT22I 2102 ATGCAT ATGCA^T
Hin1I 1390 GGCGCC G[GA]^CG[CT]C
HincII 241 GTTGAC GT[CT]^[GA]AC
HindII 241 GTTGAC GT[CT]^[GA]AC
KasI 1390 GGCGCC G^GCGCC
MluNI 941 TGGCCA TGG^CCA
MscI 941 TGGCCA TGG^CCA
MspA1I 1102 CAGCTG C[AC]G^C[GT]G
MvnI 638 CGCG CG^CG
NarI 1390 GGCGCC GG^CGCC
NsiI 2102 ATGCAT ATGCA^T
PflFI 244 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 2074 CTGCAG CTGCA^G
PvuII 1102 CAGCTG CAG^CTG
RsaI 216 GTAC GT^AC
SapI 1629 GCTCTTC GCTCTTC(1/4)
SexAI 413 ACCTGGT A^CC[AT]GGT
SfcI 2074 CTGCAG C^T[GA][CT]AG
SfoI 1390 GGCGCC GGC^GCC
Tth111I 244 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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BanII 493 GAGCTC G[GA]GC[CT]^C
1723 GAGCTC
BfaI 844 CTAG C^TAG
1705 CTAG
BglII 437 AGATCT A^GATCT
732 AGATCT
BsaI 1555 GAGACC GGTCTC(1/5)
1908 GAGACC
BspMI 1355 ACCTGC ACCTGC(4/8)
117 GCAGGT
EaeI 941 TGGCCA [CT]^GGCC[GA]
1882 CGGCCA
HaeII 1390 GGCGCC [GA]GCGC^[CT]
1694 AGCGCC
HapII 764 CCGG C^CGG
2007 CCGG
HindIII 886 AAGCTT A^AGCTT
1639 AAGCTT
HpaII 764 CCGG C^CGG
2007 CCGG
MaeI 844 CTAG C^TAG
1705 CTAG
MaeIII 332 GTGAC ^GT[ACGT]AC
547 GTCAC
MspI 764 CCGG C^CGG
2007 CCGG
NcoI 314 CCATGG C^CATGG
1406 CCATGG
NspI 573 GCATGT [GA]CATG^[CT]
1997 ACATGC
PpuMI 1043 AGGACCC [GA]G^G[AT]CC[CT]
1352 AGGACCT
SacI 493 GAGCTC GAGCT^C
1723 GAGCTC
StuI 505 AGGCCT AGG^CCT
2146 AGGCCT
Tsp45I 332 GTGAC ^GT[GC]AC
547 GTCAC
XcmI 903 CCAAGATTCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
926 CCAAGACTGTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BbvI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII