(Circular) (Six-base) MAPSORT of: pFN21ASDA0359.seq Check: 4999 from: 1 to: 6970 pFN21ASDA0359 6970 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2131 2131 Size: 6970 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4780 278 Size: 53 83 186 936 3244 2468 Fragments arranged by size: 3244 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4313 1937 Size: 2376 4594 Acc65I G'GTAC_C Cuts at: 4291 4291 Size: 6970 AclI AA'CG_TT Cuts at: 1563 5097 5470 1563 Size: 3534 373 3063 Fragments arranged by size: 3534 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3371 3650 5033 6417 6685 1632 Size: 1739 279 1383 1384 268 1917 Fragments arranged by size: 1917 1739 1384 1383 279 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6773 AflIII A'CryG_T Cuts at: 1203 5869 6846 1203 Size: 4666 977 1327 Fragments arranged by size: 4666 1327 977 AgeI A'CCGG_T Cuts at: 5801 5801 Size: 6970 AhdI GACnn_n'nnGTC Cuts at: 5699 5699 Size: 6970 AleI CACnn'nnGTG Cuts at: 1166 2540 2730 1166 Size: 1374 190 5406 Fragments arranged by size: 5406 1374 190 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6970 AlwNI CAG_nnn'CTG Cuts at: 4713 6285 4713 Size: 1572 5398 ApaLI G'TGCA_C Cuts at: 5027 6183 5027 Size: 1156 5814 ApoI r'AATT_y Cuts at: 1493 3092 4279 4505 4657 6766 1493 Size: 1599 1187 226 152 2109 1697 Fragments arranged by size: 2109 1697 1599 1187 226 152 AseI AT'TA_AT Cuts at: 160 1032 5524 160 Size: 872 4492 1606 Fragments arranged by size: 4492 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6970 AvaI C'yCGr_G Cuts at: 1870 1943 4245 4295 1870 Size: 73 2302 50 4545 Fragments arranged by size: 4545 2302 73 50 BamHI G'GATC_C Cuts at: 4193 4300 4667 4193 Size: 107 367 6496 Fragments arranged by size: 6496 367 107 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3413 4291 5746 618 Size: 325 217 627 1626 878 1455 1842 Fragments arranged by size: 1842 1626 1455 878 627 325 217 BanII G_rGCy'C Cuts at: 1391 2520 3750 4289 1391 Size: 1129 1230 539 4072 Fragments arranged by size: 4072 1230 1129 539 BbeI G_GCGC'C Cuts at: 3417 3417 Size: 6970 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2553 928 Size: 971 654 5345 Fragments arranged by size: 5345 971 654 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3515 3549 5161 5195 1903 Size: 34 1578 34 1612 34 3678 Fragments arranged by size: 3678 1612 1578 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4862 6072 4862 Size: 1210 5760 BclI T'GATC_A Cuts at: 1548 1548 Size: 6970 BfrBI ATG'CAT Cuts at: 4127 4127 Size: 6970 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5581 136 Size: 107 122 71 631 605 3909 1525 Fragments arranged by size: 3909 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2460 2755 6965 2460 Size: 295 4210 2465 Fragments arranged by size: 4210 2465 295 BlpI GC'TnA_GC Cuts at: 4387 4387 Size: 6970 Bme1580I G_kGCm'C Cuts at: 1790 5031 6187 1790 Size: 3241 1156 2573 Fragments arranged by size: 3241 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6970 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2325 4701 5659 Size: 416 53 83 399 24 908 2376 958 Cuts at: 5659 442 Size: 1753 Fragments arranged by size: 2376 1753 958 908 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6970 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2504 2674 2504 Size: 170 6800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3305 3518 3902 5630 1239 Size: 2066 213 384 1728 2579 Fragments arranged by size: 2579 2066 1728 384 213 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2534 3101 3752 5095 5960 6258 2534 Size: 567 651 1343 865 298 3246 Fragments arranged by size: 3246 1343 865 651 567 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3572 3925 5633 882 Size: 617 2073 353 1708 2219 Fragments arranged by size: 2219 2073 1708 617 353 BsaAI yAC'GTr Cuts at: 493 1204 4267 4761 493 Size: 711 3063 494 2702 Fragments arranged by size: 3063 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6964 6964 Size: 6970 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3414 4777 5159 5794 Size: 53 83 186 936 1881 1363 382 635 Cuts at: 5794 275 Size: 1451 Fragments arranged by size: 1881 1451 1363 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5403 5801 6075 6222 6633 5403 Size: 398 274 147 411 5740 Fragments arranged by size: 5740 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2532 2562 3163 3193 3425 Size: 30 165 30 1129 30 601 30 232 Cuts at: 3425 3455 1178 Size: 30 4693 Fragments arranged by size: 4693 1129 601 232 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2385 2486 2912 3221 3224 3227 3230 3248 Size: 1199 101 426 309 3 3 3 18 Cuts at: 3248 3347 3590 4189 4192 1186 Size: 99 243 599 3 3964 Fragments arranged by size: 3964 1199 599 426 309 243 101 99 18 3 3 3 3 BseYI C'CCAG_C Cuts at: 2134 3123 3409 4025 6173 6669 2134 Size: 989 286 616 2148 496 2435 Fragments arranged by size: 2435 2148 989 616 496 286 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2716 1163 Size: 573 980 5417 Fragments arranged by size: 5417 980 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4420 5181 6209 1540 Size: 275 186 2419 761 1028 2301 Fragments arranged by size: 2419 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2520 2655 3750 5031 5116 6187 2520 Size: 135 1095 1281 85 1071 3303 Fragments arranged by size: 3303 1281 1095 1071 135 85 BsmI GAATG_Cn' Cuts at: 2224 4481 4574 2224 Size: 2257 93 4620 Fragments arranged by size: 4620 2257 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2240 2240 Size: 6970 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2520 2655 3750 4289 5031 5116 6187 Size: 399 730 135 1095 539 742 85 1071 Cuts at: 6187 1391 Size: 2174 Fragments arranged by size: 2174 1095 1071 742 730 539 399 135 85 BspEI T'CCGG_A Cuts at: 6633 6633 Size: 6970 BspHI T'CATG_A Cuts at: 4859 6589 4859 Size: 1730 5240 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2131 3387 4325 844 Size: 1287 1256 938 3489 Fragments arranged by size: 3489 1287 1256 938 BsrBI CCG'CTC Cuts at: 1394 1823 4857 1394 Size: 429 3034 3507 Fragments arranged by size: 3507 3034 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2950 3608 4059 5465 5639 65 Size: 1183 1702 658 451 1406 174 1396 Fragments arranged by size: 1702 1406 1396 1183 658 451 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2786 5614 5801 1700 Size: 110 976 2828 187 2869 Fragments arranged by size: 2869 2828 976 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6970 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6970 BssSI C'ACGA_G Cuts at: 5030 6042 5030 Size: 1012 5958 BstAPI GCAn_nnn'nTGC Cuts at: 794 4088 794 Size: 3294 3676 BstBI TT'CG_AA Cuts at: 2007 5774 2007 Size: 3767 3203 BstEII G'GTnAC_C Cuts at: 1184 2354 1184 Size: 1170 5800 BstYI r'GATC_y Cuts at: 1972 2460 2475 2755 3684 4193 4300 4667 5052 Size: 488 15 280 929 509 107 367 385 Cuts at: 5052 5069 6521 6607 6619 6965 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 929 509 488 385 367 346 280 107 86 17 15 12 Bsu36I CC'TnA_GG Cuts at: 4210 4210 Size: 6970 BtgI C'CryG_G Cuts at: 513 1065 2337 2350 3429 3918 513 Size: 552 1272 13 1079 489 3565 Fragments arranged by size: 3565 1272 1079 552 489 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6231 Fragments arranged by size: 6231 683 56 BtsI GCAGTG_nn' Cuts at: 3193 3678 4143 4486 5280 5300 3193 Size: 485 465 343 794 20 4863 Fragments arranged by size: 4863 794 485 465 343 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6970 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6935 DraI TTT'AAA Cuts at: 4274 4274 Size: 6970 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5977 809 Size: 507 4661 1802 Fragments arranged by size: 4661 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2964 3905 4417 Size: 54 1425 50 275 18 1134 941 512 Cuts at: 4417 5306 6935 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1134 941 889 512 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4417 1537 Size: 275 2605 4090 Fragments arranged by size: 4090 2605 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2991 3096 3149 3198 3219 3659 3837 4210 Size: 1638 105 53 49 21 440 178 373 Cuts at: 4210 4900 1353 Size: 690 3423 Fragments arranged by size: 3423 1638 690 440 373 178 105 53 49 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5557 5931 6077 387 Size: 1064 138 3968 374 146 1280 Fragments arranged by size: 3968 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2518 3748 2518 Size: 1230 5740 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3305 3371 3518 3650 3902 5033 5630 Size: 393 1673 66 147 132 252 1131 597 Cuts at: 5630 6417 6685 1239 Size: 787 268 1524 Fragments arranged by size: 1673 1524 1131 787 597 393 268 252 147 132 66 EcoNI CCTnn'n_nnAGG Cuts at: 2535 2535 Size: 6970 EcoO109I rG'GnC_Cy Cuts at: 2975 3067 3376 2975 Size: 92 309 6569 Fragments arranged by size: 6569 309 92 EcoRI G'AATT_C Cuts at: 3092 4279 4657 3092 Size: 1187 378 5405 Fragments arranged by size: 5405 1187 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2951 2951 Size: 6970 FspI TGC'GCA Cuts at: 5476 5476 Size: 6970 HaeII r_GCGC'y Cuts at: 1678 3417 3721 6117 1678 Size: 1739 304 2396 2531 Fragments arranged by size: 2531 2396 1739 304 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2474 2506 3550 3582 3955 Size: 32 135 32 902 32 1044 32 373 Cuts at: 3955 3987 4115 4147 5611 5643 5685 5717 1373 Size: 32 128 32 1464 32 42 32 2626 Fragments arranged by size: 2626 1464 1044 902 373 135 128 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2266 4314 4560 669 Size: 988 281 328 2048 246 3079 Fragments arranged by size: 3079 2048 988 328 281 246 HindIII A'AGCT_T Cuts at: 748 2909 3662 748 Size: 2161 753 4056 Fragments arranged by size: 4056 2161 753 HpaI GTT'AAC Cuts at: 4560 4560 Size: 6970 KasI G'GCGC_C Cuts at: 3413 3413 Size: 6970 KpnI G_GTAC'C Cuts at: 4295 4295 Size: 6970 MfeI C'AATT_G Cuts at: 4569 4569 Size: 6970 MluI A'CGCG_T Cuts at: 6846 6846 Size: 6970 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2278 3430 6084 6268 1305 Size: 973 1152 2654 184 2007 Fragments arranged by size: 2654 2007 1152 973 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2966 10 Size: 54 1425 343 1134 4014 Fragments arranged by size: 4014 1425 1134 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2540 2730 3477 4928 5287 5446 518 Size: 648 1374 190 747 1451 359 159 2042 Fragments arranged by size: 2042 1451 1374 747 648 359 190 159 MspA1I CmG'CkG Cuts at: 1606 3127 4386 5063 6211 6456 1606 Size: 1521 1259 677 1148 245 2120 Fragments arranged by size: 2120 1521 1259 1148 677 245 NarI GG'CG_CC Cuts at: 3414 3414 Size: 6970 NcoI C'CATG_G Cuts at: 513 1065 2337 3429 513 Size: 552 1272 1092 4054 Fragments arranged by size: 4054 1272 1092 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6970 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6970 NotI GC'GGCC_GC Cuts at: 4417 4417 Size: 6970 NsiI A_TGCA'T Cuts at: 4129 4129 Size: 6970 NspI r_CATG'y Cuts at: 1132 1229 2600 4024 4328 5873 1132 Size: 97 1371 1424 304 1545 2229 Fragments arranged by size: 2229 1545 1424 1371 304 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6970 PciI A'CATG_T Cuts at: 5869 5869 Size: 6970 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6970 PfoI T'CCnGG_A Cuts at: 3096 3495 3096 Size: 399 6571 PmeI GTTT'AAAC Cuts at: 4274 4274 Size: 6970 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5039 5071 6552 6584 5039 Size: 32 1481 32 5425 Fragments arranged by size: 5425 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3067 3376 3067 Size: 309 6661 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6970 PsiI TTA'TAA Cuts at: 4540 4540 Size: 6970 PstI C_TGCA'G Cuts at: 830 4101 4322 830 Size: 3271 221 3478 Fragments arranged by size: 3478 3271 221 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6970 PvuII CAG'CTG Cuts at: 3127 3127 Size: 6970 SacI G_AGCT'C Cuts at: 2520 3750 2520 Size: 1230 5740 SalI G'TCGA_C Cuts at: 1936 4312 1936 Size: 2376 4594 SapI GCTCTTCn'nnn_ Cuts at: 3659 3659 Size: 6970 SbfI CC_TGCA'GG Cuts at: 4322 4322 Size: 6970 ScaI AGT'ACT Cuts at: 5218 6930 5218 Size: 1712 5258 SexAI A'CCwGG_T Cuts at: 2436 2436 Size: 6970 SfcI C'TryA_G Cuts at: 826 1045 4097 4318 5453 6134 6325 826 Size: 219 3052 221 1135 681 191 1471 Fragments arranged by size: 3052 1471 1135 681 221 219 191 SfoI GGC'GCC Cuts at: 3415 3415 Size: 6970 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6970 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6970 SmaI CCC'GGG Cuts at: 1872 4297 1872 Size: 2425 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2513 3116 3767 5074 5975 6237 Size: 197 926 570 603 651 1307 901 262 Cuts at: 6237 820 Size: 1553 Fragments arranged by size: 1553 1307 926 901 651 603 570 262 197 SnaBI TAC'GTA Cuts at: 493 4267 4761 493 Size: 3774 494 2702 Fragments arranged by size: 3774 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6970 SphI G_CATG'C Cuts at: 1132 4328 1132 Size: 3196 3774 SspI AAT'ATT Cuts at: 5 52 4894 5 Size: 47 4842 2081 Fragments arranged by size: 4842 2081 47 StuI AGG'CCT Cuts at: 1517 2530 4171 1517 Size: 1013 1641 4316 Fragments arranged by size: 4316 1641 1013 StyI C'CwwG_G Cuts at: 513 1065 2337 2935 3053 3072 3429 3582 4068 Size: 552 1272 598 118 19 357 153 486 Cuts at: 4068 4161 4409 513 Size: 93 248 3074 Fragments arranged by size: 3074 1272 598 552 486 357 248 153 118 93 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2420 4997 5014 5167 5352 5797 325 Size: 1255 840 2577 17 153 185 445 1498 Fragments arranged by size: 2577 1498 1255 840 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5216 6928 96 Size: 274 80 33 51 1443 3239 1712 138 Fragments arranged by size: 3239 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2532 2564 2875 2907 3163 3195 6930 6962 2532 Size: 32 311 32 256 32 3735 32 2540 Fragments arranged by size: 3735 2540 311 256 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2270 1316 Size: 954 6016 XbaI T'CTAG_A Cuts at: 4306 4306 Size: 6970 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2933 2956 2933 Size: 23 6947 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6970 XmaI C'CCGG_G Cuts at: 1870 4295 1870 Size: 2425 4545 XmnI GAAnn'nnTTC Cuts at: 5099 5099 Size: 6970 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4778 276 Size: 53 83 186 936 3244 2468 Fragments arranged by size: 3244 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI Bpu10I BsiWI BstXI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI