Restriction map for
pFN21ASDA0359
(Linear) (Six-base) MAPSORT of: KIAA0359.seq Check: 2447 from: 1 to: 2241
KIAA0359.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 108 2241
Size: 108 2133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1348 1627 2241
Size: 1348 279 614
Fragments arranged by size:
1348 614 279
AleI CACnn'nnGTG
Cuts at: 0 517 707 2241
Size: 517 190 1534
Fragments arranged by size:
1534 517 190
ApoI r'AATT_y
Cuts at: 0 1069 2241
Size: 1069 1172
AvaI C'yCGr_G
Cuts at: 0 2222 2241
Size: 2222 19
BamHI G'GATC_C
Cuts at: 0 2170 2241
Size: 2170 71
BanI G'GyrC_C
Cuts at: 0 1390 2241
Size: 1390 851
BanII G_rGCy'C
Cuts at: 0 497 1727 2241
Size: 497 1230 514
Fragments arranged by size:
1230 514 497
BbeI G_GCGC'C
Cuts at: 0 1394 2241
Size: 1394 847
BbsI GAAGACnn'nnnn_
Cuts at: 0 530 2241
Size: 530 1711
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1492 1526 2241
Size: 1492 34 715
Fragments arranged by size:
1492 715 34
BfrBI ATG'CAT
Cuts at: 0 2104 2241
Size: 2104 137
BglII A'GATC_T
Cuts at: 0 437 732 2241
Size: 437 295 1509
Fragments arranged by size:
1509 437 295
BmrI ACTGGGnnnn_n'
Cuts at: 0 302 2241
Size: 302 1939
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 481 651 2241
Size: 481 170 1590
Fragments arranged by size:
1590 481 170
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1282 1495 1879 2241
Size: 1282 213 384 362
Fragments arranged by size:
1282 384 362 213
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 1078 1729 2241
Size: 511 567 651 512
Fragments arranged by size:
651 567 512 511
BsaI GGTCTCn'nnnn_
Cuts at: 0 1549 1902 2241
Size: 1549 353 339
Fragments arranged by size:
1549 353 339
BsaHI Gr'CG_yC
Cuts at: 0 1391 2241
Size: 1391 850
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 509 539 1140 1170 1402 1432 2241
Size: 509 30 601 30 232 30 809
Fragments arranged by size:
809 601 509 232 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 362 463 889 1198 1201 1204 1207 1225
Size: 362 101 426 309 3 3 3 18
Cuts at: 1225 1324 1567 2166 2169 2241
Size: 99 243 599 3 72
Fragments arranged by size:
599 426 362 309 243 101 99 72
18 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 111 1100 1386 2002 2241
Size: 111 989 286 616 239
Fragments arranged by size:
989 616 286 239 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 693 2241
Size: 693 1548
BsiHKAI G_wGCw'C
Cuts at: 0 497 632 1727 2241
Size: 497 135 1095 514
Fragments arranged by size:
1095 514 497 135
BsmI GAATG_Cn'
Cuts at: 0 201 2241
Size: 201 2040
BsmBI CGTCTCn'nnnn_
Cuts at: 0 217 2241
Size: 217 2024
Bsp1286I G_dGCh'C
Cuts at: 0 497 632 1727 2241
Size: 497 135 1095 514
Fragments arranged by size:
1095 514 497 135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 108 1364 2241
Size: 108 1256 877
Fragments arranged by size:
1256 877 108
BsrDI GCAATG_nn'
Cuts at: 0 927 1585 2036 2241
Size: 927 658 451 205
Fragments arranged by size:
927 658 451 205
BsrFI r'CCGG_y
Cuts at: 0 763 2241
Size: 763 1478
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2065 2241
Size: 2065 176
BstEII G'GTnAC_C
Cuts at: 0 331 2241
Size: 331 1910
BstYI r'GATC_y
Cuts at: 0 437 452 732 1661 2170 2241
Size: 437 15 280 929 509 71
Fragments arranged by size:
929 509 437 280 71 15
Bsu36I CC'TnA_GG
Cuts at: 0 2187 2241
Size: 2187 54
BtgI C'CryG_G
Cuts at: 0 314 327 1406 1895 2241
Size: 314 13 1079 489 346
Fragments arranged by size:
1079 489 346 314 13
BtsI GCAGTG_nn'
Cuts at: 0 1170 1655 2120 2241
Size: 1170 485 465 121
Fragments arranged by size:
1170 485 465 121
EaeI y'GGCC_r
Cuts at: 0 941 1882 2241
Size: 941 941 359
Fragments arranged by size:
941 941 359
EarI CTCTTCn'nnn_
Cuts at: 0 968 1073 1126 1175 1196 1636 1814 2187
Size: 968 105 53 49 21 440 178 373
Cuts at: 2187 2241
Size: 54
Fragments arranged by size:
968 440 373 178 105 54 53 49
21
EcoICRI GAG'CTC
Cuts at: 0 495 1725 2241
Size: 495 1230 516
Fragments arranged by size:
1230 516 495
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1282 1348 1495 1627 1879 2241
Size: 1282 66 147 132 252 362
Fragments arranged by size:
1282 362 252 147 132 66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 512 2241
Size: 512 1729
EcoO109I rG'GnC_Cy
Cuts at: 0 952 1044 1353 2241
Size: 952 92 309 888
Fragments arranged by size:
952 888 309 92
EcoRI G'AATT_C
Cuts at: 0 1069 2241
Size: 1069 1172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 928 2241
Size: 928 1313
HaeII r_GCGC'y
Cuts at: 0 1394 1698 2241
Size: 1394 304 543
Fragments arranged by size:
1394 543 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 451 483 1527 1559 1932 1964 2092 2124
Size: 451 32 1044 32 373 32 128 32
Cuts at: 2124 2241
Size: 117
Fragments arranged by size:
1044 451 373 128 117 32 32 32
32
HincII GTy'rAC
Cuts at: 0 243 2241
Size: 243 1998
HindIII A'AGCT_T
Cuts at: 0 886 1639 2241
Size: 886 753 602
Fragments arranged by size:
886 753 602
KasI G'GCGC_C
Cuts at: 0 1390 2241
Size: 1390 851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 1407 2241
Size: 255 1152 834
Fragments arranged by size:
1152 834 255
MscI TGG'CCA
Cuts at: 0 943 2241
Size: 943 1298
MslI CAynn'nnrTG
Cuts at: 0 517 707 1454 2241
Size: 517 190 747 787
Fragments arranged by size:
787 747 517 190
MspA1I CmG'CkG
Cuts at: 0 1104 2241
Size: 1104 1137
NarI GG'CG_CC
Cuts at: 0 1391 2241
Size: 1391 850
NcoI C'CATG_G
Cuts at: 0 314 1406 2241
Size: 314 1092 835
Fragments arranged by size:
1092 835 314
NsiI A_TGCA'T
Cuts at: 0 2106 2241
Size: 2106 135
NspI r_CATG'y
Cuts at: 0 577 2001 2241
Size: 577 1424 240
Fragments arranged by size:
1424 577 240
PfoI T'CCnGG_A
Cuts at: 0 1073 1472 2241
Size: 1073 399 769
Fragments arranged by size:
1073 769 399
PpuMI rG'GwC_Cy
Cuts at: 0 1044 1353 2241
Size: 1044 309 888
Fragments arranged by size:
1044 888 309
PstI C_TGCA'G
Cuts at: 0 2078 2241
Size: 2078 163
PvuII CAG'CTG
Cuts at: 0 1104 2241
Size: 1104 1137
SacI G_AGCT'C
Cuts at: 0 497 1727 2241
Size: 497 1230 514
Fragments arranged by size:
1230 514 497
SapI GCTCTTCn'nnn_
Cuts at: 0 1636 2241
Size: 1636 605
SexAI A'CCwGG_T
Cuts at: 0 413 2241
Size: 413 1828
SfcI C'TryA_G
Cuts at: 0 2074 2241
Size: 2074 167
SfoI GGC'GCC
Cuts at: 0 1392 2241
Size: 1392 849
SmlI C'TyrA_G
Cuts at: 0 490 1093 1744 2241
Size: 490 603 651 497
Fragments arranged by size:
651 603 497 490
StuI AGG'CCT
Cuts at: 0 507 2148 2241
Size: 507 1641 93
Fragments arranged by size:
1641 507 93
StyI C'CwwG_G
Cuts at: 0 314 912 1030 1049 1406 1559 2045 2138
Size: 314 598 118 19 357 153 486 93
Cuts at: 2138 2241
Size: 103
Fragments arranged by size:
598 486 357 314 153 118 103 93
19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 397 2241
Size: 397 1844
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 509 541 852 884 1140 1172 2241
Size: 509 32 311 32 256 32 1069
Fragments arranged by size:
1069 509 311 256 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 247 2241
Size: 247 1994
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 910 933 2241
Size: 910 23 1308
Fragments arranged by size:
1308 910 23
Enzymes that do cut:
AarI AcuI AleI ApoI AvaI BamHI BanI BanII
BbeI BbsI BcgI BfrBI BglII BmrI BplI BpmI
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI BstAPI BstEII
BstYI Bsu36I BtgI BtsI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII
KasI MmeI MscI MslI MspA1I NarI NcoI NsiI
NspI PfoI PpuMI PstI PvuII SacI SapI SexAI
SfcI SfoI SmlI StuI StyI TaqII TstI Tth111I
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbvCI BciVI BclI BglI BlpI
Bme1580I BmgBI BmtI Bpu10I BsaAI BsaBI BsaWI BsiEI
BsiWI BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI
BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoRV FseI FspI FspAI HpaI KpnI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TatI XbaI XhoI
XmaI XmnI ZraI