(Circular) (Six-base) MAPSORT of: pFN21ASDA0353.seq Check: 223 from: 1 to: 9424 pFN21ASDA0353 9424 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2908 3455 4475 2908 Size: 547 1020 7857 Fragments arranged by size: 7857 1020 547 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7234 278 Size: 53 83 186 936 5698 2468 Fragments arranged by size: 5698 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6106 6630 6767 1937 Size: 4169 524 137 4594 Fragments arranged by size: 4594 4169 524 137 Acc65I G'GTAC_C Cuts at: 5792 6745 5792 Size: 953 8471 AclI AA'CG_TT Cuts at: 1563 7551 7924 1563 Size: 5988 373 3063 Fragments arranged by size: 5988 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3983 5046 5200 5235 5439 7487 8871 9139 Size: 2351 1063 154 35 204 2048 1384 268 Cuts at: 9139 1632 Size: 1917 Fragments arranged by size: 2351 2048 1917 1384 1063 268 204 154 35 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9227 AflIII A'CryG_T Cuts at: 1203 2097 8323 9300 1203 Size: 894 6226 977 1327 Fragments arranged by size: 6226 1327 977 894 AgeI A'CCGG_T Cuts at: 3842 8255 3842 Size: 4413 5011 AhdI GACnn_n'nnGTC Cuts at: 6637 8153 6637 Size: 1516 7908 AleI CACnn'nnGTG Cuts at: 1166 4031 1166 Size: 2865 6559 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9424 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3292 3324 3292 Size: 32 9392 AlwNI CAG_nnn'CTG Cuts at: 2233 2314 4867 5162 5371 5636 7167 8739 2233 Size: 81 2553 295 209 265 1531 1572 2918 Fragments arranged by size: 2918 2553 1572 1531 295 265 209 81 ApaI G_GGCC'C Cuts at: 2051 5315 5738 6387 2051 Size: 3264 423 649 5088 Fragments arranged by size: 5088 3264 649 423 ApaLI G'TGCA_C Cuts at: 2570 6050 6074 7481 8637 2570 Size: 3480 24 1407 1156 3357 Fragments arranged by size: 3480 3357 1407 1156 24 ApoI r'AATT_y Cuts at: 1493 3029 4766 4817 6733 6959 7111 9220 1493 Size: 1536 1737 51 1916 226 152 2109 1697 Fragments arranged by size: 2109 1916 1737 1697 1536 226 152 51 AseI AT'TA_AT Cuts at: 160 1032 7978 160 Size: 872 6946 1606 Fragments arranged by size: 6946 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9424 AvaI C'yCGr_G Cuts at: 1870 1943 2051 2411 2747 3223 4538 4904 5232 Size: 73 108 360 336 476 1315 366 328 Cuts at: 5232 5290 6100 6749 1870 Size: 58 810 649 4545 Fragments arranged by size: 4545 1315 810 649 476 366 360 336 328 108 73 58 AvrII C'CTAG_G Cuts at: 5590 5590 Size: 9424 BamHI G'GATC_C Cuts at: 6754 7121 6754 Size: 367 9057 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3162 3751 3884 5792 6745 Size: 325 217 627 1375 589 133 1908 953 Cuts at: 6745 8200 618 Size: 1455 1842 Fragments arranged by size: 1908 1842 1455 1375 953 627 589 325 217 133 BanII G_rGCy'C Cuts at: 1391 2051 2067 2076 3224 4278 4857 5315 5513 Size: 660 16 9 1148 1054 579 458 198 Cuts at: 5513 5627 5738 6065 6387 6466 6743 1391 Size: 114 111 327 322 79 277 4072 Fragments arranged by size: 4072 1148 1054 660 579 458 327 322 277 198 114 111 79 16 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2930 5643 928 Size: 971 1031 2713 4709 Fragments arranged by size: 4709 2713 1031 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2345 2379 3453 3487 3790 3824 4622 Size: 34 408 34 1074 34 303 34 798 Cuts at: 4622 4656 5607 5641 7615 7649 1903 Size: 34 951 34 1974 34 3678 Fragments arranged by size: 3678 1974 1074 951 798 408 303 34 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2805 3781 3968 7316 8526 2805 Size: 976 187 3348 1210 3703 Fragments arranged by size: 3703 3348 1210 976 187 BclI T'GATC_A Cuts at: 1548 1548 Size: 9424 BfrBI ATG'CAT Cuts at: 2515 2515 Size: 9424 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2210 2414 5349 Size: 107 122 71 631 605 538 204 2935 Cuts at: 5349 5971 8035 136 Size: 622 2064 1525 Fragments arranged by size: 2935 2064 1525 631 622 605 538 204 122 107 71 BglII A'GATC_T Cuts at: 9419 9419 Size: 9424 BlpI GC'TnA_GC Cuts at: 2243 3003 5078 6064 6841 2243 Size: 760 2075 986 777 4826 Fragments arranged by size: 4826 2075 986 777 760 Bme1580I G_kGCm'C Cuts at: 1790 2051 2574 3165 3185 3887 4548 5315 5738 Size: 261 523 591 20 702 661 767 423 Cuts at: 5738 6054 6078 6387 7485 8641 1790 Size: 316 24 309 1098 1156 2573 Fragments arranged by size: 2573 1156 1098 767 702 661 591 523 423 316 309 261 24 20 BmgBI CAC'GTC Cuts at: 1318 2476 1318 Size: 1158 8266 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5309 5353 6029 Size: 416 53 83 399 24 3892 44 676 Cuts at: 6029 7155 8113 442 Size: 1126 958 1753 Fragments arranged by size: 3892 1753 1126 958 676 416 399 83 53 44 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9424 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3733 3733 Size: 9424 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2138 2159 2669 2795 2870 2960 3966 Size: 812 87 21 510 126 75 90 1006 Cuts at: 3966 4509 4736 4910 5090 5393 5446 5801 6511 Size: 543 227 174 180 303 53 355 710 Cuts at: 6511 6602 8084 1239 Size: 91 1482 2579 Fragments arranged by size: 2579 1482 1006 812 710 543 510 355 303 227 180 174 126 91 90 87 75 53 21 Bpu10I CC'TnA_GC Cuts at: 3705 5138 6330 6365 3705 Size: 1433 1192 35 6764 Fragments arranged by size: 6764 1433 1192 35 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3327 7549 8414 8712 3327 Size: 4222 865 298 4039 Fragments arranged by size: 4222 4039 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6552 8087 882 Size: 617 5053 1535 2219 Fragments arranged by size: 5053 2219 1535 617 BsaAI yAC'GTr Cuts at: 493 1204 2098 4033 4465 6721 7215 493 Size: 711 894 1935 432 2256 494 2702 Fragments arranged by size: 2702 2256 1935 894 711 494 432 BsaBI GATnn'nnATC Cuts at: 2993 4583 5055 9418 2993 Size: 1590 472 4363 2999 Fragments arranged by size: 4363 2999 1590 472 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2352 3815 7231 7613 Size: 53 83 186 936 819 1463 3416 382 Cuts at: 7613 8248 275 Size: 635 1451 Fragments arranged by size: 3416 1463 1451 936 819 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3842 5516 7857 8255 8529 8676 9087 3842 Size: 1674 2341 398 274 147 411 4179 Fragments arranged by size: 4179 2341 1674 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3292 3322 3964 3994 4910 Size: 30 165 30 1889 30 642 30 916 Cuts at: 4910 4940 5892 5922 1178 Size: 30 952 30 4680 Fragments arranged by size: 4680 1889 952 916 642 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2156 2159 2222 2339 2666 2816 2837 3635 Size: 970 3 63 117 327 150 21 798 Cuts at: 3635 4286 4322 4349 4394 4604 4834 4934 5087 Size: 651 36 27 45 210 230 100 153 Cuts at: 5087 6662 6704 6707 6710 1186 Size: 1575 42 3 3 3900 Fragments arranged by size: 3900 1575 970 798 651 327 230 210 153 150 117 100 63 45 42 36 27 21 3 3 3 BseYI C'CCAG_C Cuts at: 2247 2359 2392 2422 3249 4702 5155 5548 5641 Size: 112 33 30 827 1453 453 393 93 Cuts at: 5641 5985 6548 8627 9123 2247 Size: 344 563 2079 496 2548 Fragments arranged by size: 2548 2079 1453 827 563 496 453 393 344 112 93 33 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2639 4652 4686 6195 6632 1163 Size: 573 903 2013 34 1509 437 3955 Fragments arranged by size: 3955 2013 1509 903 573 437 34 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2158 2344 5059 5517 6874 7635 Size: 275 186 157 186 2715 458 1357 761 Cuts at: 7635 8663 1540 Size: 1028 2301 Fragments arranged by size: 2715 2301 1357 1028 761 458 275 186 186 157 BsiHKAI G_wGCw'C Cuts at: 2067 2076 2574 3009 6047 6054 6078 6134 6466 Size: 9 498 435 3038 7 24 56 332 Cuts at: 6466 7485 7570 8641 2067 Size: 1019 85 1071 2850 Fragments arranged by size: 3038 2850 1071 1019 498 435 332 85 56 24 9 7 BsmI GAATG_Cn' Cuts at: 5208 6935 7028 5208 Size: 1727 93 7604 Fragments arranged by size: 7604 1727 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2606 2693 5041 6489 2606 Size: 87 2348 1448 5541 Fragments arranged by size: 5541 2348 1448 87 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2067 2076 2574 3009 3165 3185 Size: 399 261 16 9 498 435 156 20 Cuts at: 3185 3224 3887 4278 4548 4857 5315 5513 5627 Size: 39 663 391 270 309 458 198 114 Cuts at: 5627 5738 6047 6054 6065 6078 6134 6387 6466 Size: 111 309 7 11 13 56 253 79 Cuts at: 6466 6743 7485 7570 8641 1391 Size: 277 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 663 498 458 435 399 391 309 309 277 270 261 253 198 156 114 111 85 79 56 39 20 16 13 11 9 7 BspEI T'CCGG_A Cuts at: 9087 9087 Size: 9424 BspHI T'CATG_A Cuts at: 7313 9043 7313 Size: 1730 7694 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2908 3455 4475 6779 844 Size: 2064 547 1020 2304 3489 Fragments arranged by size: 3489 2304 2064 1020 547 BsrBI CCG'CTC Cuts at: 1394 1823 2275 2827 5189 7311 1394 Size: 429 452 552 2362 2122 3507 Fragments arranged by size: 3507 2362 2122 552 452 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6270 6372 7919 8093 65 Size: 1183 5022 102 1547 174 1396 Fragments arranged by size: 5022 1547 1396 1183 174 102 BsrFI r'CCGG_y Cuts at: 1700 1810 2157 2534 2927 3842 5526 8068 8255 Size: 110 347 377 393 915 1684 2542 187 Cuts at: 8255 1700 Size: 2869 Fragments arranged by size: 2869 2542 1684 915 393 377 347 187 110 BsrGI T'GTAC_A Cuts at: 96 5241 5908 96 Size: 5145 667 3612 Fragments arranged by size: 5145 3612 667 BssHII G'CGCG_C Cuts at: 1925 2213 2329 2397 2399 2401 2488 2490 2492 Size: 288 116 68 2 2 87 2 2 Cuts at: 2492 2494 2712 2764 2766 1925 Size: 2 218 52 2 8583 Fragments arranged by size: 8583 288 218 116 87 68 52 2 2 2 2 2 2 BssSI C'ACGA_G Cuts at: 3535 7484 8496 3535 Size: 3949 1012 4463 Fragments arranged by size: 4463 3949 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3467 794 Size: 2673 6751 BstBI TT'CG_AA Cuts at: 2007 8228 2007 Size: 6221 3203 BstEII G'GTnAC_C Cuts at: 1184 5489 1184 Size: 4305 5119 BstXI CCAn_nnnn'nTGG Cuts at: 2260 4362 4997 5101 5619 6524 2260 Size: 2102 635 104 518 905 5160 Fragments arranged by size: 5160 2102 905 635 518 104 BstYI r'GATC_y Cuts at: 1972 3170 4578 6218 6754 7121 7506 7523 8975 Size: 1198 1408 1640 536 367 385 17 1452 Cuts at: 8975 9061 9073 9419 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1640 1452 1408 1198 536 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2338 2880 3166 4971 6001 6280 6481 Size: 552 1273 542 286 1805 1030 279 201 Cuts at: 6481 513 Size: 3456 Fragments arranged by size: 3456 1805 1273 1030 552 542 286 279 201 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4416 487 Size: 683 56 3190 5495 Fragments arranged by size: 5495 3190 683 56 BtsI GCAGTG_nn' Cuts at: 2253 2585 4674 5457 6940 7734 7754 2253 Size: 332 2089 783 1483 794 20 3923 Fragments arranged by size: 3923 2089 1483 794 783 332 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9424 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4771 4806 5335 5370 529 Size: 35 4207 35 529 35 4583 Fragments arranged by size: 4583 4207 529 35 35 35 DraI TTT'AAA Cuts at: 6728 6728 Size: 9424 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4022 8431 809 Size: 507 2706 4409 1802 Fragments arranged by size: 4409 2706 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2155 2341 2595 Size: 54 1425 50 275 18 325 186 254 Cuts at: 2595 2668 2877 3240 5322 6871 7760 9389 8 Size: 73 209 363 2082 1549 889 1629 43 Fragments arranged by size: 2082 1629 1549 1425 889 363 325 275 254 209 186 73 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2155 2341 6871 1537 Size: 275 343 186 4530 4090 Fragments arranged by size: 4530 4090 343 275 186 EarI CTCTTCn'nnn_ Cuts at: 1353 2691 3202 3579 3725 3778 5775 7354 1353 Size: 1338 511 377 146 53 1997 1579 3423 Fragments arranged by size: 3423 1997 1579 1338 511 377 146 53 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2279 2705 4918 8011 8385 8531 Size: 1064 138 690 426 2213 3093 374 146 Cuts at: 8531 387 Size: 1280 Fragments arranged by size: 3093 2213 1280 1064 690 426 374 146 138 EcoICRI GAG'CTC Cuts at: 2065 2074 6464 2065 Size: 9 4390 5025 Fragments arranged by size: 5025 4390 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2138 2159 2669 2795 2870 2960 Size: 393 419 87 21 510 126 75 90 Cuts at: 2960 3966 3983 4509 4736 4910 5046 5090 5200 Size: 1006 17 526 227 174 136 44 110 Cuts at: 5200 5235 5393 5439 5446 5801 6511 6602 7487 Size: 35 158 46 7 355 710 91 885 Cuts at: 7487 8084 8871 9139 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1006 885 787 710 597 526 510 419 393 355 268 227 174 158 136 126 110 91 90 87 75 46 44 35 21 17 7 EcoNI CCTnn'n_nnAGG Cuts at: 2141 2564 2915 5498 5832 6333 2141 Size: 423 351 2583 334 501 5232 Fragments arranged by size: 5232 2583 501 423 351 334 EcoO109I rG'GnC_Cy Cuts at: 2048 2173 2908 3391 4358 4723 5194 5277 5311 Size: 125 735 483 967 365 471 83 34 Cuts at: 5311 5453 5552 5594 5734 5735 5825 6242 6383 Size: 142 99 42 140 1 90 417 141 Cuts at: 6383 2048 Size: 5089 Fragments arranged by size: 5089 967 735 483 471 417 365 142 141 140 125 99 90 83 42 34 1 EcoRI G'AATT_C Cuts at: 3029 4817 6733 7111 3029 Size: 1788 1916 378 5342 Fragments arranged by size: 5342 1916 1788 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6151 6151 Size: 9424 FspI TGC'GCA Cuts at: 2367 7930 2367 Size: 5563 3861 HaeII r_GCGC'y Cuts at: 1678 2314 2393 2420 2446 2660 2732 2747 4709 Size: 636 79 27 26 214 72 15 1962 Cuts at: 4709 5162 8571 1678 Size: 453 3409 2531 Fragments arranged by size: 3409 2531 1962 636 453 214 79 72 27 26 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4194 4226 4233 4265 4547 Size: 32 135 32 2622 32 7 32 282 Cuts at: 4547 4579 4580 4612 4910 4942 5426 5458 5568 Size: 32 1 32 298 32 484 32 110 Cuts at: 5568 5600 6623 6655 8065 8097 8139 8171 1373 Size: 32 1023 32 1410 32 42 32 2626 Fragments arranged by size: 2626 2622 1410 1023 484 298 282 135 110 42 32 32 32 32 32 32 32 32 32 32 32 32 7 1 HincII GTy'rAC Cuts at: 669 1657 1938 4255 5032 6768 7014 669 Size: 988 281 2317 777 1736 246 3079 Fragments arranged by size: 3079 2317 1736 988 777 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9424 HpaI GTT'AAC Cuts at: 7014 7014 Size: 9424 KpnI G_GTAC'C Cuts at: 5796 6749 5796 Size: 953 8471 MfeI C'AATT_G Cuts at: 3420 7023 3420 Size: 3603 5821 MluI A'CGCG_T Cuts at: 9300 9300 Size: 9424 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2733 3335 8538 8722 1305 Size: 1428 602 5203 184 2007 Fragments arranged by size: 5203 2007 1428 602 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2879 10 Size: 54 1425 343 1047 6555 Fragments arranged by size: 6555 1425 1047 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4031 4829 7382 7741 7900 518 Size: 648 2865 798 2553 359 159 2042 Fragments arranged by size: 2865 2553 2042 798 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2199 2233 2340 2623 2761 4293 4867 4888 Size: 593 34 107 283 138 1532 574 21 Cuts at: 4888 6320 6840 7517 8665 8910 1606 Size: 1432 520 677 1148 245 2120 Fragments arranged by size: 2120 1532 1432 1148 677 593 574 520 283 245 138 107 34 21 NaeI GCC'GGC Cuts at: 2159 2159 Size: 9424 NcoI C'CATG_G Cuts at: 513 1065 2880 6001 513 Size: 552 1815 3121 3936 Fragments arranged by size: 3936 3121 1815 552 NdeI CA'TA_TG Cuts at: 387 5446 387 Size: 5059 4365 NgoMIV G'CCGG_C Cuts at: 2157 2157 Size: 9424 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9424 NotI GC'GGCC_GC Cuts at: 2341 6871 2341 Size: 4530 4894 NsiI A_TGCA'T Cuts at: 2517 2517 Size: 9424 NspI r_CATG'y Cuts at: 1132 1229 3022 5543 6782 8327 1132 Size: 97 1793 2521 1239 1545 2229 Fragments arranged by size: 2521 2229 1793 1545 1239 97 PasI CC'CwG_GG Cuts at: 1348 4362 5308 1348 Size: 3014 946 5464 Fragments arranged by size: 5464 3014 946 PciI A'CATG_T Cuts at: 8323 8323 Size: 9424 PflMI CCAn_nnn'nTGG Cuts at: 1108 3317 4740 5162 5636 6007 6625 1108 Size: 2209 1423 422 474 371 618 3907 Fragments arranged by size: 3907 2209 1423 618 474 422 371 PfoI T'CCnGG_A Cuts at: 2067 2136 6488 2067 Size: 69 4352 5003 Fragments arranged by size: 5003 4352 69 PmeI GTTT'AAAC Cuts at: 6728 6728 Size: 9424 PmlI CAC'GTG Cuts at: 4465 4465 Size: 9424 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3292 3324 3578 3610 3857 3889 7493 7525 9006 Size: 32 254 32 247 32 3604 32 1481 Cuts at: 9006 9038 3292 Size: 32 3678 Fragments arranged by size: 3678 3604 1481 254 247 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2908 3391 4723 5453 5552 6242 2908 Size: 483 1332 730 99 690 6090 Fragments arranged by size: 6090 1332 730 690 483 99 PshAI GACnn'nnGTC Cuts at: 1613 5496 5804 6289 1613 Size: 3883 308 485 4748 Fragments arranged by size: 4748 3883 485 308 PsiI TTA'TAA Cuts at: 6994 6994 Size: 9424 PspOMI G'GGCC_C Cuts at: 2047 5311 5734 6383 2047 Size: 3264 423 649 5088 Fragments arranged by size: 5088 3264 649 423 PspXI vC'TCGA_Gb Cuts at: 2411 2411 Size: 9424 PstI C_TGCA'G Cuts at: 830 2043 2310 2385 2436 2919 5464 5659 5699 Size: 1213 267 75 51 483 2545 195 40 Cuts at: 5699 5752 6776 830 Size: 53 1024 3478 Fragments arranged by size: 3478 2545 1213 1024 483 267 195 75 53 51 40 PvuI CG_AT'CG Cuts at: 2001 5059 2001 Size: 3058 6366 PvuII CAG'CTG Cuts at: 2233 2623 2761 4867 4888 2233 Size: 390 138 2106 21 6769 Fragments arranged by size: 6769 2106 390 138 21 SacI G_AGCT'C Cuts at: 2067 2076 6466 2067 Size: 9 4390 5025 Fragments arranged by size: 5025 4390 9 SacII CC_GC'GG Cuts at: 2341 2341 Size: 9424 SalI G'TCGA_C Cuts at: 1936 6766 1936 Size: 4830 4594 SanDI GG'GwC_CC Cuts at: 5552 5552 Size: 9424 SapI GCTCTTCn'nnn_ Cuts at: 3778 3778 Size: 9424 SbfI CC_TGCA'GG Cuts at: 2919 6776 2919 Size: 3857 5567 ScaI AGT'ACT Cuts at: 7672 9384 7672 Size: 1712 7712 SfcI C'TryA_G Cuts at: 826 1045 2039 2306 2381 2432 2915 3068 5460 Size: 219 994 267 75 51 483 153 2392 Cuts at: 5460 5655 5695 5748 6246 6772 7907 8588 8779 Size: 195 40 53 498 526 1135 681 191 Cuts at: 8779 826 Size: 1471 Fragments arranged by size: 2392 1471 1135 994 681 526 498 483 267 219 195 191 153 75 53 51 40 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9424 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9424 SmaI CCC'GGG Cuts at: 1872 4540 5234 6751 1872 Size: 2668 694 1517 4545 Fragments arranged by size: 4545 2668 1517 694 SmlI C'TyrA_G Cuts at: 820 1017 1943 2411 3342 7528 8429 8691 820 Size: 197 926 468 931 4186 901 262 1553 Fragments arranged by size: 4186 1553 931 926 901 468 262 197 SnaBI TAC'GTA Cuts at: 493 6721 7215 493 Size: 6228 494 2702 Fragments arranged by size: 6228 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9424 SphI G_CATG'C Cuts at: 1132 6782 1132 Size: 5650 3774 SspI AAT'ATT Cuts at: 5 52 7348 5 Size: 47 7296 2081 Fragments arranged by size: 7296 2081 47 StuI AGG'CCT Cuts at: 1517 2451 6542 1517 Size: 934 4091 4399 Fragments arranged by size: 4399 4091 934 StyI C'CwwG_G Cuts at: 513 1065 2874 2880 4692 4848 5590 6001 6863 Size: 552 1809 6 1812 156 742 411 862 Cuts at: 6863 513 Size: 3074 Fragments arranged by size: 3074 1812 1809 862 742 552 411 156 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2351 5879 5999 7451 7468 7621 7806 Size: 1255 771 3528 120 1452 17 153 185 Cuts at: 7806 8251 325 Size: 445 1498 Fragments arranged by size: 3528 1498 1452 1255 771 445 185 153 120 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5241 5908 7670 Size: 274 80 33 51 1443 3264 667 1762 Cuts at: 7670 9382 96 Size: 1712 138 Fragments arranged by size: 3264 1762 1712 1443 667 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3964 3996 5536 5568 5746 5778 9384 9416 3964 Size: 32 1540 32 178 32 3606 32 3972 Fragments arranged by size: 3972 3606 1540 178 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6240 1316 Size: 4924 4500 XbaI T'CTAG_A Cuts at: 3763 6679 6760 3763 Size: 2916 81 6427 Fragments arranged by size: 6427 2916 81 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4997 5464 4997 Size: 467 8957 XhoI C'TCGA_G Cuts at: 1943 2411 1943 Size: 468 8956 XmaI C'CCGG_G Cuts at: 1870 4538 5232 6749 1870 Size: 2668 694 1517 4545 Fragments arranged by size: 4545 2668 1517 694 XmnI GAAnn'nnTTC Cuts at: 3353 3593 3968 4899 7553 3353 Size: 240 375 931 2654 5224 Fragments arranged by size: 5224 2654 931 375 240 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7232 276 Size: 53 83 186 936 5698 2468 Fragments arranged by size: 5698 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BbvCI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI PacI PsrI RsrII SexAI SfiI SfoI SrfI SwaI