Restriction map for
pFN21ASDA0353
(Linear) (Six-base) MAPSORT of: KIAA0353.seq Check: 8773 from: 1 to: 4695
KIAA0353.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 885 1432 2452 4695
Size: 885 547 1020 2243
Fragments arranged by size:
2243 1020 885 547
AccI GT'mk_AC
Cuts at: 0 4083 4607 4695
Size: 4083 524 88
Fragments arranged by size:
4083 524 88
Acc65I G'GTAC_C
Cuts at: 0 3769 4695
Size: 3769 926
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1960 3023 3177 3212 3416 4695
Size: 1960 1063 154 35 204 1279
Fragments arranged by size:
1960 1279 1063 204 154 35
AflIII A'CryG_T
Cuts at: 0 74 4695
Size: 74 4621
AgeI A'CCGG_T
Cuts at: 0 1819 4695
Size: 1819 2876
AhdI GACnn_n'nnGTC
Cuts at: 0 4614 4695
Size: 4614 81
AleI CACnn'nnGTG
Cuts at: 0 2008 4695
Size: 2008 2687
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1269 1301 4695
Size: 1269 32 3394
Fragments arranged by size:
3394 1269 32
AlwNI CAG_nnn'CTG
Cuts at: 0 210 291 2844 3139 3348 3613 4695
Size: 210 81 2553 295 209 265 1082
Fragments arranged by size:
2553 1082 295 265 210 209 81
ApaI G_GGCC'C
Cuts at: 0 28 3292 3715 4364 4695
Size: 28 3264 423 649 331
Fragments arranged by size:
3264 649 423 331 28
ApaLI G'TGCA_C
Cuts at: 0 547 4027 4051 4695
Size: 547 3480 24 644
Fragments arranged by size:
3480 644 547 24
ApoI r'AATT_y
Cuts at: 0 1006 2743 2794 4695
Size: 1006 1737 51 1901
Fragments arranged by size:
1901 1737 1006 51
AvaI C'yCGr_G
Cuts at: 0 28 388 724 1200 2515 2881 3209 3267
Size: 28 360 336 476 1315 366 328 58
Cuts at: 3267 4077 4695
Size: 810 618
Fragments arranged by size:
1315 810 618 476 366 360 336 328
58 28
AvrII C'CTAG_G
Cuts at: 0 3567 4695
Size: 3567 1128
BanI G'GyrC_C
Cuts at: 0 1139 1728 1861 3769 4695
Size: 1139 589 133 1908 926
Fragments arranged by size:
1908 1139 926 589 133
BanII G_rGCy'C
Cuts at: 0 28 44 53 1201 2255 2834 3292 3490
Size: 28 16 9 1148 1054 579 458 198
Cuts at: 3490 3604 3715 4042 4364 4443 4695
Size: 114 111 327 322 79 252
Fragments arranged by size:
1148 1054 579 458 327 322 252 198
114 111 79 28 16 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 907 3620 4695
Size: 907 2713 1075
Fragments arranged by size:
2713 1075 907
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 322 356 1430 1464 1767 1801 2599 2633
Size: 322 34 1074 34 303 34 798 34
Cuts at: 2633 3584 3618 4695
Size: 951 34 1077
Fragments arranged by size:
1077 1074 951 798 322 303 34 34
34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 782 1758 1945 4695
Size: 782 976 187 2750
Fragments arranged by size:
2750 976 782 187
BfrBI ATG'CAT
Cuts at: 0 492 4695
Size: 492 4203
BglI GCCn_nnn'nGGC
Cuts at: 0 187 391 3326 3948 4695
Size: 187 204 2935 622 747
Fragments arranged by size:
2935 747 622 204 187
BlpI GC'TnA_GC
Cuts at: 0 220 980 3055 4041 4695
Size: 220 760 2075 986 654
Fragments arranged by size:
2075 986 760 654 220
Bme1580I G_kGCm'C
Cuts at: 0 28 551 1142 1162 1864 2525 3292 3715
Size: 28 523 591 20 702 661 767 423
Cuts at: 3715 4031 4055 4364 4695
Size: 316 24 309 331
Fragments arranged by size:
767 702 661 591 523 423 331 316
309 28 24 20
BmgBI CAC'GTC
Cuts at: 0 453 4695
Size: 453 4242
BmrI ACTGGGnnnn_n'
Cuts at: 0 3286 3330 4006 4695
Size: 3286 44 676 689
Fragments arranged by size:
3286 689 676 44
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1710 4695
Size: 1710 2985
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 115 136 646 772 847 937 1943
Size: 28 87 21 510 126 75 90 1006
Cuts at: 1943 2486 2713 2887 3067 3370 3423 3778 4488
Size: 543 227 174 180 303 53 355 710
Cuts at: 4488 4579 4695
Size: 91 116
Fragments arranged by size:
1006 710 543 510 355 303 227 180
174 126 116 91 90 87 75 53
28 21
Bpu10I CC'TnA_GC
Cuts at: 0 1682 3115 4307 4342 4695
Size: 1682 1433 1192 35 353
Fragments arranged by size:
1682 1433 1192 353 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1304 4695
Size: 1304 3391
BsaI GGTCTCn'nnnn_
Cuts at: 0 4529 4695
Size: 4529 166
BsaAI yAC'GTr
Cuts at: 0 75 2010 2442 4695
Size: 75 1935 432 2253
Fragments arranged by size:
2253 1935 432 75
BsaBI GATnn'nnATC
Cuts at: 0 970 2560 3032 4695
Size: 970 1590 472 1663
Fragments arranged by size:
1663 1590 970 472
BsaHI Gr'CG_yC
Cuts at: 0 329 1792 4695
Size: 329 1463 2903
Fragments arranged by size:
2903 1463 329
BsaWI w'CCGG_w
Cuts at: 0 1819 3493 4695
Size: 1819 1674 1202
Fragments arranged by size:
1819 1674 1202
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1269 1299 1941 1971 2887 2917 3869 3899
Size: 1269 30 642 30 916 30 952 30
Cuts at: 3899 4695
Size: 796
Fragments arranged by size:
1269 952 916 796 642 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 136 199 316 643 793 814 1612
Size: 133 3 63 117 327 150 21 798
Cuts at: 1612 2263 2299 2326 2371 2581 2811 2911 3064
Size: 651 36 27 45 210 230 100 153
Cuts at: 3064 4639 4681 4684 4687 4695
Size: 1575 42 3 3 8
Fragments arranged by size:
1575 798 651 327 230 210 153 150
133 117 100 63 45 42 36 27
21 8 3 3 3
BseYI C'CCAG_C
Cuts at: 0 224 336 369 399 1226 2679 3132 3525
Size: 224 112 33 30 827 1453 453 393
Cuts at: 3525 3618 3962 4525 4695
Size: 93 344 563 170
Fragments arranged by size:
1453 827 563 453 393 344 224 170
112 93 33 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 2629 2663 4172 4609 4695
Size: 616 2013 34 1509 437 86
Fragments arranged by size:
2013 1509 616 437 86 34
BsiEI CG_ry'CG
Cuts at: 0 135 321 3036 3494 4695
Size: 135 186 2715 458 1201
Fragments arranged by size:
2715 1201 458 186 135
BsiHKAI G_wGCw'C
Cuts at: 0 44 53 551 986 4024 4031 4055 4111
Size: 44 9 498 435 3038 7 24 56
Cuts at: 4111 4443 4695
Size: 332 252
Fragments arranged by size:
3038 498 435 332 252 56 44 24
9 7
BsmI GAATG_Cn'
Cuts at: 0 3185 4695
Size: 3185 1510
BsmBI CGTCTCn'nnnn_
Cuts at: 0 583 670 3018 4466 4695
Size: 583 87 2348 1448 229
Fragments arranged by size:
2348 1448 583 229 87
Bsp1286I G_dGCh'C
Cuts at: 0 28 44 53 551 986 1142 1162 1201
Size: 28 16 9 498 435 156 20 39
Cuts at: 1201 1864 2255 2525 2834 3292 3490 3604 3715
Size: 663 391 270 309 458 198 114 111
Cuts at: 3715 4024 4031 4042 4055 4111 4364 4443 4695
Size: 309 7 11 13 56 253 79 252
Fragments arranged by size:
663 498 458 435 391 309 309 270
253 252 198 156 114 111 79 56
39 28 20 16 13 11 9 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 885 1432 2452 4695
Size: 885 547 1020 2243
Fragments arranged by size:
2243 1020 885 547
BsrBI CCG'CTC
Cuts at: 0 252 804 3166 4695
Size: 252 552 2362 1529
Fragments arranged by size:
2362 1529 552 252
BsrDI GCAATG_nn'
Cuts at: 0 4247 4349 4695
Size: 4247 102 346
Fragments arranged by size:
4247 346 102
BsrFI r'CCGG_y
Cuts at: 0 134 511 904 1819 3503 4695
Size: 134 377 393 915 1684 1192
Fragments arranged by size:
1684 1192 915 393 377 134
BsrGI T'GTAC_A
Cuts at: 0 3218 3885 4695
Size: 3218 667 810
Fragments arranged by size:
3218 810 667
BssHII G'CGCG_C
Cuts at: 0 190 306 374 376 378 465 467 469
Size: 190 116 68 2 2 87 2 2
Cuts at: 469 471 689 741 743 4695
Size: 2 218 52 2 3952
Fragments arranged by size:
3952 218 190 116 87 68 52 2
2 2 2 2 2
BssSI C'ACGA_G
Cuts at: 0 1512 4695
Size: 1512 3183
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1444 4695
Size: 1444 3251
BstEII G'GTnAC_C
Cuts at: 0 3466 4695
Size: 3466 1229
BstXI CCAn_nnnn'nTGG
Cuts at: 0 237 2339 2974 3078 3596 4501 4695
Size: 237 2102 635 104 518 905 194
Fragments arranged by size:
2102 905 635 518 237 194 104
BstYI r'GATC_y
Cuts at: 0 1147 2555 4195 4695
Size: 1147 1408 1640 500
Fragments arranged by size:
1640 1408 1147 500
BtgI C'CryG_G
Cuts at: 0 315 857 1143 2948 3978 4257 4458 4695
Size: 315 542 286 1805 1030 279 201 237
Fragments arranged by size:
1805 1030 542 315 286 279 237 201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2393 4695
Size: 2393 2302
BtsI GCAGTG_nn'
Cuts at: 0 230 562 2651 3434 4695
Size: 230 332 2089 783 1261
Fragments arranged by size:
2089 1261 783 332 230
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2748 2783 3312 3347 4695
Size: 2748 35 529 35 1348
Fragments arranged by size:
2748 1348 529 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1999 4695
Size: 1999 2696
EaeI y'GGCC_r
Cuts at: 0 132 318 572 645 854 1217 3299 4695
Size: 132 186 254 73 209 363 2082 1396
Fragments arranged by size:
2082 1396 363 254 209 186 132 73
EagI C'GGCC_G
Cuts at: 0 132 318 4695
Size: 132 186 4377
Fragments arranged by size:
4377 186 132
EarI CTCTTCn'nnn_
Cuts at: 0 668 1179 1556 1702 1755 3752 4695
Size: 668 511 377 146 53 1997 943
Fragments arranged by size:
1997 943 668 511 377 146 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 256 682 2895 4695
Size: 256 426 2213 1800
Fragments arranged by size:
2213 1800 426 256
EcoICRI GAG'CTC
Cuts at: 0 42 51 4441 4695
Size: 42 9 4390 254
Fragments arranged by size:
4390 254 42 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 115 136 646 772 847 937 1943
Size: 28 87 21 510 126 75 90 1006
Cuts at: 1943 1960 2486 2713 2887 3023 3067 3177 3212
Size: 17 526 227 174 136 44 110 35
Cuts at: 3212 3370 3416 3423 3778 4488 4579 4695
Size: 158 46 7 355 710 91 116
Fragments arranged by size:
1006 710 526 510 355 227 174 158
136 126 116 110 91 90 87 75
46 44 35 28 21 17 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 118 541 892 3475 3809 4310 4695
Size: 118 423 351 2583 334 501 385
Fragments arranged by size:
2583 501 423 385 351 334 118
EcoO109I rG'GnC_Cy
Cuts at: 0 25 150 885 1368 2335 2700 3171 3254
Size: 25 125 735 483 967 365 471 83
Cuts at: 3254 3288 3430 3529 3571 3711 3712 3802 4219
Size: 34 142 99 42 140 1 90 417
Cuts at: 4219 4360 4695
Size: 141 335
Fragments arranged by size:
967 735 483 471 417 365 335 142
141 140 125 99 90 83 42 34
25 1
EcoRI G'AATT_C
Cuts at: 0 1006 2794 4695
Size: 1006 1788 1901
Fragments arranged by size:
1901 1788 1006
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4128 4695
Size: 4128 567
FspI TGC'GCA
Cuts at: 0 344 4695
Size: 344 4351
HaeII r_GCGC'y
Cuts at: 0 291 370 397 423 637 709 724 2686
Size: 291 79 27 26 214 72 15 1962
Cuts at: 2686 3139 4695
Size: 453 1556
Fragments arranged by size:
1962 1556 453 291 214 79 72 27
26 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2171 2203 2210 2242 2524 2556 2557 2589
Size: 2171 32 7 32 282 32 1 32
Cuts at: 2589 2887 2919 3403 3435 3545 3577 4600 4632
Size: 298 32 484 32 110 32 1023 32
Cuts at: 4632 4695
Size: 63
Fragments arranged by size:
2171 1023 484 298 282 110 63 32
32 32 32 32 32 32 32 7
1
HincII GTy'rAC
Cuts at: 0 2232 3009 4695
Size: 2232 777 1686
Fragments arranged by size:
2232 1686 777
KpnI G_GTAC'C
Cuts at: 0 3773 4695
Size: 3773 922
MfeI C'AATT_G
Cuts at: 0 1397 4695
Size: 1397 3298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 1312 4695
Size: 710 602 3383
Fragments arranged by size:
3383 710 602
MscI TGG'CCA
Cuts at: 0 856 4695
Size: 856 3839
MslI CAynn'nnrTG
Cuts at: 0 2008 2806 4695
Size: 2008 798 1889
Fragments arranged by size:
2008 1889 798
MspA1I CmG'CkG
Cuts at: 0 176 210 317 600 738 2270 2844 2865
Size: 176 34 107 283 138 1532 574 21
Cuts at: 2865 4297 4695
Size: 1432 398
Fragments arranged by size:
1532 1432 574 398 283 176 138 107
34 21
NaeI GCC'GGC
Cuts at: 0 136 4695
Size: 136 4559
NcoI C'CATG_G
Cuts at: 0 857 3978 4695
Size: 857 3121 717
Fragments arranged by size:
3121 857 717
NdeI CA'TA_TG
Cuts at: 0 3423 4695
Size: 3423 1272
NgoMIV G'CCGG_C
Cuts at: 0 134 4695
Size: 134 4561
NotI GC'GGCC_GC
Cuts at: 0 318 4695
Size: 318 4377
NsiI A_TGCA'T
Cuts at: 0 494 4695
Size: 494 4201
NspI r_CATG'y
Cuts at: 0 999 3520 4695
Size: 999 2521 1175
Fragments arranged by size:
2521 1175 999
PasI CC'CwG_GG
Cuts at: 0 2339 3285 4695
Size: 2339 946 1410
Fragments arranged by size:
2339 1410 946
PflMI CCAn_nnn'nTGG
Cuts at: 0 1294 2717 3139 3613 3984 4602 4695
Size: 1294 1423 422 474 371 618 93
Fragments arranged by size:
1423 1294 618 474 422 371 93
PfoI T'CCnGG_A
Cuts at: 0 44 113 4465 4695
Size: 44 69 4352 230
Fragments arranged by size:
4352 230 69 44
PmlI CAC'GTG
Cuts at: 0 2442 4695
Size: 2442 2253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1269 1301 1555 1587 1834 1866 4695
Size: 1269 32 254 32 247 32 2829
Fragments arranged by size:
2829 1269 254 247 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 885 1368 2700 3430 3529 4219 4695
Size: 885 483 1332 730 99 690 476
Fragments arranged by size:
1332 885 730 690 483 476 99
PshAI GACnn'nnGTC
Cuts at: 0 3473 3781 4266 4695
Size: 3473 308 485 429
Fragments arranged by size:
3473 485 429 308
PspOMI G'GGCC_C
Cuts at: 0 24 3288 3711 4360 4695
Size: 24 3264 423 649 335
Fragments arranged by size:
3264 649 423 335 24
PspXI vC'TCGA_Gb
Cuts at: 0 388 4695
Size: 388 4307
PstI C_TGCA'G
Cuts at: 0 20 287 362 413 896 3441 3636 3676
Size: 20 267 75 51 483 2545 195 40
Cuts at: 3676 3729 4695
Size: 53 966
Fragments arranged by size:
2545 966 483 267 195 75 53 51
40 20
PvuI CG_AT'CG
Cuts at: 0 3036 4695
Size: 3036 1659
PvuII CAG'CTG
Cuts at: 0 210 600 738 2844 2865 4695
Size: 210 390 138 2106 21 1830
Fragments arranged by size:
2106 1830 390 210 138 21
SacI G_AGCT'C
Cuts at: 0 44 53 4443 4695
Size: 44 9 4390 252
Fragments arranged by size:
4390 252 44 9
SacII CC_GC'GG
Cuts at: 0 318 4695
Size: 318 4377
SanDI GG'GwC_CC
Cuts at: 0 3529 4695
Size: 3529 1166
SapI GCTCTTCn'nnn_
Cuts at: 0 1755 4695
Size: 1755 2940
SbfI CC_TGCA'GG
Cuts at: 0 896 4695
Size: 896 3799
SfcI C'TryA_G
Cuts at: 0 16 283 358 409 892 1045 3437 3632
Size: 16 267 75 51 483 153 2392 195
Cuts at: 3632 3672 3725 4223 4695
Size: 40 53 498 472
Fragments arranged by size:
2392 498 483 472 267 195 153 75
53 51 40 16
SmaI CCC'GGG
Cuts at: 0 2517 3211 4695
Size: 2517 694 1484
Fragments arranged by size:
2517 1484 694
SmlI C'TyrA_G
Cuts at: 0 388 1319 4695
Size: 388 931 3376
Fragments arranged by size:
3376 931 388
StuI AGG'CCT
Cuts at: 0 428 4519 4695
Size: 428 4091 176
Fragments arranged by size:
4091 428 176
StyI C'CwwG_G
Cuts at: 0 851 857 2669 2825 3567 3978 4695
Size: 851 6 1812 156 742 411 717
Fragments arranged by size:
1812 851 742 717 411 156 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 328 3856 3976 4695
Size: 328 3528 120 719
Fragments arranged by size:
3528 719 328 120
TatI w'GTAC_w
Cuts at: 0 3218 3885 4695
Size: 3218 667 810
Fragments arranged by size:
3218 810 667
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1941 1973 3513 3545 3723 3755 4695
Size: 1941 32 1540 32 178 32 940
Fragments arranged by size:
1941 1540 940 178 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4217 4695
Size: 4217 478
XbaI T'CTAG_A
Cuts at: 0 1740 4656 4695
Size: 1740 2916 39
Fragments arranged by size:
2916 1740 39
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2974 3441 4695
Size: 2974 467 1254
Fragments arranged by size:
2974 1254 467
XhoI C'TCGA_G
Cuts at: 0 388 4695
Size: 388 4307
XmaI C'CCGG_G
Cuts at: 0 2515 3209 4695
Size: 2515 694 1486
Fragments arranged by size:
2515 1486 694
XmnI GAAnn'nnTTC
Cuts at: 0 1330 1570 1945 2876 4695
Size: 1330 240 375 931 1819
Fragments arranged by size:
1819 1330 931 375 240
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AgeI AhdI AleI
AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BanI
BanII BbsI BcgI BciVI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI
BtgZI BtsI CspCI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KpnI MfeI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NotI NsiI NspI PasI
PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII SacI SacII SanDI SapI SbfI
SfcI SmaI SmlI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AlfI AscI AseI AsiSI
BaeI BamHI BbeI BbvCI BclI BglII BmtI BsiWI
BspEI BspHI BstBI BstZ17I Bsu36I ClaI DraI DraIII
EcoRV FseI FspAI HindIII HpaI KasI MluI NarI
NheI NruI PacI PciI PmeI PsiI PsrI RsrII
SalI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI ZraI