Restriction map for
pFN21ASDA0352
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AccII AccIII AclI AflII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI AspEI AspI AviII BbeI
BbrPI BciVI BfrI BglII BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FauI FseI FspI
HincII HindII HindIII HinfI HpaI
KasI KspI MamI MfeI MluI
MroI MunI MvnI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PleI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SnaBI SpeI Sse8387I SwaI TfiI
Tth111I
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 91 GACGTC GACGT^C
Acc65I 427 GGTACC G^GTACC
AcsI 1855 GAATTC [GA]^AATT[CT]
ApaI 1970 GGGCCC GGGCC^C
ApoI 1855 GAATTC [GA]^AATT[CT]
Asp700I 168 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 427 GGTACC G^GTACC
AvrII 687 CCTAGG C^CTAGG
BalI 1379 TGGCCA TGG^CCA
BamHI 1874 GGATCC G^GATCC
BanI 427 GGTACC G^G[CT][GA]CC
BbvCI 928 GCTGAGG CCTCAGC(-5/-2)
BcgI 1080 GCAGCATGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 287 TGATCA T^GATCA
BglI 127 GCCCACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 687 CCTAGG C^CTAGG
BlpI 316 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 316 GCTGAGC GC^T[ACGT]AGC
Bsp1407I 2093 TGTACA T^GTACA
BspHI 2024 TCATGA T^CATGA
BsrDI 752 GCAATG GCAATG(2/0)
BsrGI 2093 TGTACA T^GTACA
BssSI 720 CACGAG CACGAG(-5/-1)
CelII 316 GCTGAGC GC^T[ACGT]AGC
EaeI 1379 TGGCCA [CT]^GGCC[GA]
EcoRI 1855 GAATTC G^AATTC
FbaI 287 TGATCA T^GATCA
HaeII 1782 GGCGCT [GA]GCGC^[CT]
KpnI 427 GGTACC GGTAC^C
MluNI 1379 TGGCCA TGG^CCA
MscI 1379 TGGCCA TGG^CCA
NaeI 68 GCCGGC GCC^GGC
NgoMIV 68 GCCGGC G^CCGGC
PaeR7I 886 CTCGAG C^TCGAG
PflMI 1590 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1456 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2024 TCATGA T^CATGA
SapI 1774 GAAGAGC GCTCTTC(1/4)
SgrAI 1867 CACCGGCG C[GA]^CCGG[CT]G
SmaI 1157 CCCGGG CCC^GGG
SmlI 886 CTCGAG C^T[CT][GA]AG
SphI 1083 GCATGC GCATG^C
SspBI 2093 TGTACA T^GTACA
SspI 1266 AATATT AAT^ATT
Tsp509I 1856 AATT ^AATT
Van91I 1590 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 200 CCAGGACCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 886 CTCGAG C^TCGAG
XmaCI 1157 CCCGGG C^CCGGG
XmaI 1157 CCCGGG C^CCGGG
XmnI 168 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 259 GTCTAC GT^[AC][GT]AC
799 GTAGAC
AcyI 91 GACGTC G[GA]^CG[CT]C
1603 GACGCC
AflIII 1208 ACATGT A^C[GA][CT]GT
2034 ACATGT
Alw44I 1585 GTGCAC G^TGCAC
2044 GTGCAC
ApaLI 1585 GTGCAC G^TGCAC
2044 GTGCAC
BsaHI 91 GACGTC G[GA]^CG[CT]C
1603 GACGCC
BspLU11I 1208 ACATGT A^CATGT
2034 ACATGT
BsrBI 114 CCGCTC CCGCTC(-3/-3)
1932 CCGCTC
BsrFI 68 GCCGGC [GA]^CCGG[CT]
1868 ACCGGC
BstXI 98 CCATTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
459 CCATCCCCTTGG
BstYI 852 AGATCC [GA]^GATC[CT]
1874 GGATCC
CfoI 1358 GCGC GCG^C
1783 GCGC
Cfr10I 68 GCCGGC [GA]^CCGG[CT]
1868 ACCGGC
EcoNI 45 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
339 CCTCCTCCAGG
HhaI 1358 GCGC GCG^C
1783 GCGC
Hin1I 91 GACGTC G[GA]^CG[CT]C
1603 GACGCC
HinP1I 1358 GCGC G^CGC
1783 GCGC
MflI 852 AGATCC [GA]^GATC[CT]
1874 GGATCC
MseI 1469 TTAA T^TAA
2018 TTAA
PvuII 152 CAGCTG CAG^CTG
1240 CAGCTG
SacI 723 GAGCTC GAGCT^C
877 GAGCTC
StuI 347 AGGCCT AGG^CCT
1439 AGGCCT
Tru9I 1469 TTAA T^TAA
2018 TTAA
XbaI 1053 TCTAGA T^CTAGA
1566 TCTAGA
XhoII 852 AGATCC [GA]^GATC[CT]
1874 GGATCC
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I TspRI
TthHB8I