(Circular) (Six-base) MAPSORT of: pFN21ASDA0352.seq Check: 9563 from: 1 to: 6865 pFN21ASDA0352 6865 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2118 4675 278 Size: 53 83 186 936 582 2557 2468 Fragments arranged by size: 2557 2468 936 582 186 83 53 AccI GT'mk_AC Cuts at: 1937 2283 2823 4208 1937 Size: 346 540 1385 4594 Fragments arranged by size: 4594 1385 540 346 Acc65I G'GTAC_C Cuts at: 2450 4186 2450 Size: 1736 5129 AclI AA'CG_TT Cuts at: 1563 4992 5365 1563 Size: 3429 373 3063 Fragments arranged by size: 3429 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2093 2274 2770 4928 6312 6580 1632 Size: 461 181 496 2158 1384 268 1917 Fragments arranged by size: 2158 1917 1384 496 461 268 181 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6668 AflIII A'CryG_T Cuts at: 1203 3231 4057 5764 6741 1203 Size: 2028 826 1707 977 1327 Fragments arranged by size: 2028 1707 1327 977 826 AgeI A'CCGG_T Cuts at: 5696 5696 Size: 6865 AhdI GACnn_n'nnGTC Cuts at: 5594 5594 Size: 6865 AleI CACnn'nnGTG Cuts at: 1166 2123 1166 Size: 957 5908 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6865 AlwNI CAG_nnn'CTG Cuts at: 2477 3474 3674 4608 6180 2477 Size: 997 200 934 1572 3162 Fragments arranged by size: 3162 1572 997 934 200 ApaI G_GGCC'C Cuts at: 3997 3997 Size: 6865 ApaLI G'TGCA_C Cuts at: 3608 4067 4922 6078 3608 Size: 459 855 1156 4395 Fragments arranged by size: 4395 1156 855 459 ApoI r'AATT_y Cuts at: 1493 3878 4174 4400 4552 6661 1493 Size: 2385 296 226 152 2109 1697 Fragments arranged by size: 2385 2109 1697 296 226 152 AseI AT'TA_AT Cuts at: 160 1032 5419 160 Size: 872 4387 1606 Fragments arranged by size: 4387 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6865 AvaI C'yCGr_G Cuts at: 1870 1943 2909 3111 3180 3988 4190 1870 Size: 73 966 202 69 808 202 4545 Fragments arranged by size: 4545 966 808 202 202 73 69 AvrII C'CTAG_G Cuts at: 2710 2710 Size: 6865 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2434 2467 2434 Size: 33 6832 BamHI G'GATC_C Cuts at: 3897 4195 4562 3897 Size: 298 367 6200 Fragments arranged by size: 6200 367 298 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2450 4186 5641 618 Size: 325 217 627 663 1736 1455 1842 Fragments arranged by size: 1842 1736 1455 663 627 325 217 BanII G_rGCy'C Cuts at: 1391 2750 2904 3084 3997 4184 1391 Size: 1359 154 180 913 187 4072 Fragments arranged by size: 4072 1359 913 187 180 154 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2658 2949 3535 3630 928 Size: 971 759 291 586 95 4163 Fragments arranged by size: 4163 971 759 586 291 95 BbvCI CC'TCA_GC Cuts at: 2952 2952 Size: 6865 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3092 3126 5056 5090 1903 Size: 34 1155 34 1930 34 3678 Fragments arranged by size: 3678 1930 1155 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4757 5967 4757 Size: 1210 5655 BclI T'GATC_A Cuts at: 1548 2310 1548 Size: 762 6103 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2156 5476 136 Size: 107 122 71 631 605 484 3320 1525 Fragments arranged by size: 3320 1525 631 605 484 122 107 71 BglII A'GATC_T Cuts at: 6860 6860 Size: 6865 BlpI GC'TnA_GC Cuts at: 2340 4282 2340 Size: 1942 4923 Bme1580I G_kGCm'C Cuts at: 1790 3612 3997 4026 4071 4926 6082 1790 Size: 1822 385 29 45 855 1156 2573 Fragments arranged by size: 2573 1822 1156 855 385 45 29 BmgBI CAC'GTC Cuts at: 1318 2679 3691 1318 Size: 1361 1012 4492 Fragments arranged by size: 4492 1361 1012 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2217 2541 2844 Size: 416 53 83 399 24 800 324 303 Cuts at: 2844 3250 4596 5554 442 Size: 406 1346 958 1753 Fragments arranged by size: 1753 1346 958 800 416 406 399 324 303 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6865 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2461 2461 Size: 6865 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2351 2417 2577 2888 3743 5525 1239 Size: 1112 66 160 311 855 1782 2579 Fragments arranged by size: 2579 1782 1112 855 311 160 66 Bpu10I CC'TnA_GC Cuts at: 2952 2952 Size: 6865 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4990 5855 6153 4990 Size: 865 298 5702 Fragments arranged by size: 5702 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2096 3149 3350 5528 882 Size: 617 597 1053 201 2178 2219 Fragments arranged by size: 2219 2178 1053 617 597 201 BsaAI yAC'GTr Cuts at: 493 1204 4162 4656 493 Size: 711 2958 494 2702 Fragments arranged by size: 2958 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6859 6859 Size: 6865 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2115 3627 4672 5054 Size: 53 83 186 936 582 1512 1045 382 Cuts at: 5054 5689 275 Size: 635 1451 Fragments arranged by size: 1512 1451 1045 936 635 582 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5298 5696 5970 6117 6528 5298 Size: 398 274 147 411 5635 Fragments arranged by size: 5635 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2653 2683 3161 3191 1178 Size: 30 165 30 1250 30 478 30 4852 Fragments arranged by size: 4852 1250 478 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2354 2987 3797 1186 Size: 1168 633 810 4254 Fragments arranged by size: 4254 1168 810 633 BseYI C'CCAG_C Cuts at: 2700 3340 3751 6068 6564 2700 Size: 640 411 2317 496 3001 Fragments arranged by size: 3001 2317 640 496 411 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2193 3670 3994 1163 Size: 573 457 1477 324 4034 Fragments arranged by size: 4034 1477 573 457 324 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4315 5076 6104 1540 Size: 275 186 2314 761 1028 2301 Fragments arranged by size: 2314 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2405 2750 2868 2904 3612 4071 4926 5011 6082 Size: 345 118 36 708 459 855 85 1071 Cuts at: 6082 2405 Size: 3188 Fragments arranged by size: 3188 1071 855 708 459 345 118 85 36 BsmI GAATG_Cn' Cuts at: 4376 4469 4376 Size: 93 6772 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2405 2750 2868 2904 3084 3612 3997 Size: 399 615 345 118 36 180 528 385 Cuts at: 3997 4026 4071 4184 4926 5011 6082 1391 Size: 29 45 113 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 615 528 399 385 345 180 118 113 85 45 36 29 BspEI T'CCGG_A Cuts at: 6528 6528 Size: 6865 BspHI T'CATG_A Cuts at: 4047 4754 6484 4047 Size: 707 1730 4428 Fragments arranged by size: 4428 1730 707 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2076 2485 3135 4220 844 Size: 1232 409 650 1085 3489 Fragments arranged by size: 3489 1232 1085 650 409 BsrBI CCG'CTC Cuts at: 1394 1823 2139 3957 4752 1394 Size: 429 316 1818 795 3507 Fragments arranged by size: 3507 1818 795 429 316 BsrDI GCAATG_nn' Cuts at: 65 1248 2782 5360 5534 65 Size: 1183 1534 2578 174 1396 Fragments arranged by size: 2578 1534 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2091 3891 5509 5696 1700 Size: 110 281 1800 1618 187 2869 Fragments arranged by size: 2869 1800 1618 281 187 110 BsrGI T'GTAC_A Cuts at: 96 4116 96 Size: 4020 2845 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6865 BssSI C'ACGA_G Cuts at: 2743 4925 5937 2743 Size: 2182 1012 3671 Fragments arranged by size: 3671 2182 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2477 3150 3474 794 Size: 1683 673 324 4185 Fragments arranged by size: 4185 1683 673 324 BstBI TT'CG_AA Cuts at: 2007 5669 2007 Size: 3662 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6865 BstXI CCAn_nnnn'nTGG Cuts at: 2128 2489 2128 Size: 361 6504 BstYI r'GATC_y Cuts at: 1972 2875 3897 4195 4562 4947 4964 6416 6502 Size: 903 1022 298 367 385 17 1452 86 Cuts at: 6502 6514 6860 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1022 903 385 367 346 298 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 5356 Fragments arranged by size: 5356 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6126 Fragments arranged by size: 6126 683 56 BtsI GCAGTG_nn' Cuts at: 2403 3475 3637 4381 5175 5195 2403 Size: 1072 162 744 794 20 4073 Fragments arranged by size: 4073 1072 794 744 162 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6865 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3832 3867 529 Size: 35 3268 35 3527 Fragments arranged by size: 3527 3268 35 35 DraI TTT'AAA Cuts at: 4169 4169 Size: 6865 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5872 809 Size: 507 4556 1802 Fragments arranged by size: 4556 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3402 4312 5201 Size: 54 1425 50 275 18 1572 910 889 Cuts at: 5201 6830 8 Size: 1629 43 Fragments arranged by size: 1629 1572 1425 910 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4312 1537 Size: 275 2500 4090 Fragments arranged by size: 4090 2500 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2201 2303 2571 3638 3792 4795 1353 Size: 848 102 268 1067 154 1003 3423 Fragments arranged by size: 3423 1067 1003 848 268 154 102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3910 4154 5452 5826 5972 387 Size: 1064 138 2321 244 1298 374 146 1280 Fragments arranged by size: 2321 1298 1280 1064 374 244 146 138 EcoICRI GAG'CTC Cuts at: 2748 2902 2748 Size: 154 6711 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2274 2351 2417 2577 2770 2888 Size: 393 461 181 77 66 160 193 118 Cuts at: 2888 3743 4928 5525 6312 6580 1239 Size: 855 1185 597 787 268 1524 Fragments arranged by size: 1524 1185 855 787 597 461 393 268 193 181 160 118 77 66 EcoNI CCTnn'n_nnAGG Cuts at: 2072 2366 2072 Size: 294 6571 EcoO109I rG'GnC_Cy Cuts at: 2226 2359 2914 3993 3994 2226 Size: 133 555 1079 1 5097 Fragments arranged by size: 5097 1079 555 133 1 EcoRI G'AATT_C Cuts at: 3878 4174 4552 3878 Size: 296 378 6191 Fragments arranged by size: 6191 378 296 FspI TGC'GCA Cuts at: 5371 5371 Size: 6865 HaeII r_GCGC'y Cuts at: 1678 3809 6012 1678 Size: 2131 2203 2531 Fragments arranged by size: 2531 2203 2131 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3161 3193 5506 5538 5580 Size: 32 135 32 1589 32 2313 32 42 Cuts at: 5580 5612 1373 Size: 32 2626 Fragments arranged by size: 2626 2313 1589 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4209 4455 669 Size: 988 281 2271 246 3079 Fragments arranged by size: 3079 2271 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6865 HpaI GTT'AAC Cuts at: 4455 4455 Size: 6865 KpnI G_GTAC'C Cuts at: 2454 4190 2454 Size: 1736 5129 MfeI C'AATT_G Cuts at: 4464 4464 Size: 6865 MluI A'CGCG_T Cuts at: 6741 6741 Size: 6865 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5979 6163 1305 Size: 4674 184 2007 Fragments arranged by size: 4674 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3404 10 Size: 54 1425 343 1572 3471 Fragments arranged by size: 3471 1572 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2123 2126 3605 3922 4062 4823 Size: 648 861 96 3 1479 317 140 761 Cuts at: 4823 5182 5341 518 Size: 359 159 2042 Fragments arranged by size: 2042 1479 861 761 648 359 317 159 140 96 3 MspA1I CmG'CkG Cuts at: 1606 2177 3265 4008 4281 4958 6106 6351 1606 Size: 571 1088 743 273 677 1148 245 2120 Fragments arranged by size: 2120 1148 1088 743 677 571 273 245 NaeI GCC'GGC Cuts at: 2093 2093 Size: 6865 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5356 Fragments arranged by size: 5356 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6865 NgoMIV G'CCGG_C Cuts at: 2091 2091 Size: 6865 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6865 NotI GC'GGCC_GC Cuts at: 4312 4312 Size: 6865 NspI r_CATG'y Cuts at: 1132 1229 3110 3235 4061 4223 5768 1132 Size: 97 1881 125 826 162 1545 2229 Fragments arranged by size: 2229 1881 1545 826 162 125 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6865 PciI A'CATG_T Cuts at: 3231 4057 5764 3231 Size: 826 1707 4332 Fragments arranged by size: 4332 1707 826 PflMI CCAn_nnn'nTGG Cuts at: 1108 3619 1108 Size: 2511 4354 PfoI T'CCnGG_A Cuts at: 3165 3165 Size: 6865 PmeI GTTT'AAAC Cuts at: 4169 4169 Size: 6865 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4934 4966 6447 6479 4934 Size: 32 1481 32 5320 Fragments arranged by size: 5320 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2226 2359 2914 2226 Size: 133 555 6177 Fragments arranged by size: 6177 555 133 PshAI GACnn'nnGTC Cuts at: 1613 3483 1613 Size: 1870 4995 PsiI TTA'TAA Cuts at: 4435 4435 Size: 6865 PspOMI G'GGCC_C Cuts at: 3993 3993 Size: 6865 PspXI vC'TCGA_Gb Cuts at: 2909 2909 Size: 6865 PstI C_TGCA'G Cuts at: 830 3470 3503 3747 3813 4217 830 Size: 2640 33 244 66 404 3478 Fragments arranged by size: 3478 2640 404 244 66 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6865 PvuII CAG'CTG Cuts at: 2177 3265 2177 Size: 1088 5777 SacI G_AGCT'C Cuts at: 2750 2904 2750 Size: 154 6711 SalI G'TCGA_C Cuts at: 1936 4207 1936 Size: 2271 4594 SapI GCTCTTCn'nnn_ Cuts at: 3792 3792 Size: 6865 SbfI CC_TGCA'GG Cuts at: 4217 4217 Size: 6865 ScaI AGT'ACT Cuts at: 5113 6825 5113 Size: 1712 5153 SfcI C'TryA_G Cuts at: 826 1045 3466 3499 3743 3809 4027 4213 5348 Size: 219 2421 33 244 66 218 186 1135 Cuts at: 5348 6029 6220 826 Size: 681 191 1471 Fragments arranged by size: 2421 1471 1135 681 244 219 218 191 186 66 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6865 SgrAI Cr'CCGG_yG Cuts at: 1700 3891 1700 Size: 2191 4674 SmaI CCC'GGG Cuts at: 1872 3182 4192 1872 Size: 1310 1010 4545 Fragments arranged by size: 4545 1310 1010 SmlI C'TyrA_G Cuts at: 820 1017 1943 2909 4969 5870 6132 820 Size: 197 926 966 2060 901 262 1553 Fragments arranged by size: 2060 1553 966 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4162 4656 493 Size: 3669 494 2702 Fragments arranged by size: 3669 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6865 SphI G_CATG'C Cuts at: 1132 3110 4223 1132 Size: 1978 1113 3774 Fragments arranged by size: 3774 1978 1113 SspI AAT'ATT Cuts at: 5 52 3291 4789 5 Size: 47 3239 1498 2081 Fragments arranged by size: 3239 2081 1498 47 StuI AGG'CCT Cuts at: 1517 2372 3464 1517 Size: 855 1092 4918 Fragments arranged by size: 4918 1092 855 StyI C'CwwG_G Cuts at: 513 1065 2022 2488 2518 2710 4304 513 Size: 552 957 466 30 192 1594 3074 Fragments arranged by size: 3074 1594 957 552 466 192 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3186 3765 3782 4892 4909 5062 5247 Size: 1255 1606 579 17 1110 17 153 185 Cuts at: 5247 5692 325 Size: 445 1498 Fragments arranged by size: 1606 1498 1255 1110 579 445 185 153 17 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4116 5111 6823 Size: 274 80 33 51 1443 2139 995 1712 Cuts at: 6823 96 Size: 138 Fragments arranged by size: 2139 1712 1443 995 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2653 2668 2685 2700 3506 3538 4016 4048 6825 Size: 15 17 15 806 32 478 32 2777 Cuts at: 6825 6857 2653 Size: 32 2661 Fragments arranged by size: 2777 2661 806 478 32 32 32 17 15 15 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6865 XbaI T'CTAG_A Cuts at: 3076 3589 4201 3076 Size: 513 612 5740 Fragments arranged by size: 5740 612 513 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2230 2230 Size: 6865 XhoI C'TCGA_G Cuts at: 1943 2909 1943 Size: 966 5899 XmaI C'CCGG_G Cuts at: 1870 3180 4190 1870 Size: 1310 1010 4545 Fragments arranged by size: 4545 1310 1010 XmnI GAAnn'nnTTC Cuts at: 2195 4994 2195 Size: 2799 4066 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2116 4673 276 Size: 53 83 186 936 582 2557 2468 Fragments arranged by size: 2557 2468 936 582 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI